Restriction map for
pF1KB4329
(Circular) (Six-base) MAPSORT of: pF1KB4329.seq Check: 8209 from: 1 to: 5924
pF1KB4329 5924 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1903 2163 1903
Size: 260 5664
AccI GT'mk_AC
Cuts at: 452 2897 2962 452
Size: 2445 65 3414
Fragments arranged by size:
3414 2445 65
Acc65I G'GTAC_C
Cuts at: 2940 2940
Size: 5924
AclI AA'CG_TT
Cuts at: 2918 5764 2918
Size: 2846 3078
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 2538 2641 2800 2875 2891 3749 4181 4534
Size: 1568 103 159 75 16 858 432 353
Cuts at: 4534 5196 970
Size: 662 1698
Fragments arranged by size:
1698 1568 858 662 432 353 159 103
75 16
AfeI AGC'GCT
Cuts at: 1276 1276
Size: 5924
AflIII A'CryG_T
Cuts at: 3276 5076 5357 3276
Size: 1800 281 3843
Fragments arranged by size:
3843 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 783 783
Size: 5924
AleI CACnn'nnGTG
Cuts at: 1543 2856 1543
Size: 1313 4611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 552 1561 552
Size: 1009 4915
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 5892
AlwNI CAG_nnn'CTG
Cuts at: 1170 3229 4667 1170
Size: 2059 1438 2427
Fragments arranged by size:
2427 2059 1438
ApaI G_GGCC'C
Cuts at: 2485 2485
Size: 5924
ApaLI G'TGCA_C
Cuts at: 4762 4762
Size: 5924
ApoI r'AATT_y
Cuts at: 1868 2928 5277 5533 1868
Size: 1060 2349 256 2259
Fragments arranged by size:
2349 2259 1060 256
AseI AT'TA_AT
Cuts at: 20 719 20
Size: 699 5225
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5924
AvaI C'yCGr_G
Cuts at: 1439 2944 1439
Size: 1505 4419
AvrII C'CTAG_G
Cuts at: 2073 2073
Size: 5924
BamHI G'GATC_C
Cuts at: 507 2949 507
Size: 2442 3482
BanI G'GyrC_C
Cuts at: 1688 2214 2940 3354 3602 3637 1688
Size: 526 726 414 248 35 3975
Fragments arranged by size:
3975 726 526 414 248 35
BanII G_rGCy'C
Cuts at: 2485 2868 2938 3968 2485
Size: 383 70 1030 4441
Fragments arranged by size:
4441 1030 383 70
BbeI G_GCGC'C
Cuts at: 1692 3358 3606 1692
Size: 1666 248 4010
Fragments arranged by size:
4010 1666 248
BbsI GAAGACnn'nnnn_
Cuts at: 1068 2538 2607 1068
Size: 1470 69 4385
Fragments arranged by size:
4385 1470 69
BciVI GTATCCnnnnn_n'
Cuts at: 2830 3822 4878 5924 2830
Size: 992 1056 1046 2830
Fragments arranged by size:
2830 1056 1046 992
BclI T'GATC_A
Cuts at: 3444 3444
Size: 5924
BglI GCCn_nnn'nGGC
Cuts at: 364 652 1928 364
Size: 288 1276 4360
Fragments arranged by size:
4360 1276 288
BglII A'GATC_T
Cuts at: 3439 5476 3439
Size: 2037 3887
BlpI GC'TnA_GC
Cuts at: 3036 3036
Size: 5924
Bme1580I G_kGCm'C
Cuts at: 2312 2485 3549 3642 4766 2312
Size: 173 1064 93 1124 3470
Fragments arranged by size:
3470 1124 1064 173 93
BmrI ACTGGGnnnn_n'
Cuts at: 2017 2479 3136 3217 3552 5696 2017
Size: 462 657 81 335 2144 2245
Fragments arranged by size:
2245 2144 657 462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4291 4291
Size: 5924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1537 2500 1537
Size: 963 4961
Bpu10I CC'TnA_GC
Cuts at: 1163 1163
Size: 5924
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 1570 1647 1961 3100 3979 4693 4991 1133
Size: 437 77 314 1139 879 714 298 2066
Fragments arranged by size:
2066 1139 879 714 437 314 298 77
BsaI GGTCTCn'nnnn_
Cuts at: 1661 5628 1661
Size: 3967 1957
BsaAI yAC'GTr
Cuts at: 2818 3277 2818
Size: 459 5465
BsaBI GATnn'nnATC
Cuts at: 6 401 506 623 769 3438 5475 6
Size: 395 105 117 146 2669 2037 455
Fragments arranged by size:
2669 2037 455 395 146 117 105
BsaHI Gr'CG_yC
Cuts at: 1689 3355 3603 4305 5801 1689
Size: 1666 248 702 1496 1812
Fragments arranged by size:
1812 1666 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2284 3113 3634 4312 4723 4870 5144 2284
Size: 829 521 678 411 147 274 3064
Fragments arranged by size:
3064 829 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2124 2154 2124
Size: 30 5894
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1771 2093 2402 1771
Size: 322 309 5293
Fragments arranged by size:
5293 322 309
BseYI C'CCAG_C
Cuts at: 2404 3349 4107 4772 5180 2404
Size: 945 758 665 408 3148
Fragments arranged by size:
3148 945 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1567 1021
Size: 546 5378
BsiEI CG_ry'CG
Cuts at: 104 3466 3512 4742 104
Size: 3362 46 1230 1286
Fragments arranged by size:
3362 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1906 2868 2938 3716 3906 4766 1906
Size: 962 70 778 190 860 3064
Fragments arranged by size:
3064 962 860 778 190 70
BsmI GAATG_Cn'
Cuts at: 751 751
Size: 5924
BsmBI CGTCTCn'nnnn_
Cuts at: 1753 1753
Size: 5924
Bsp1286I G_dGCh'C
Cuts at: 1906 2312 2485 2868 2938 3549 3642 3716 3906
Size: 406 173 383 70 611 93 74 190
Cuts at: 3906 3968 4766 1906
Size: 62 798 3064
Fragments arranged by size:
3064 798 611 406 383 190 173 93
74 70 62
BspEI T'CCGG_A
Cuts at: 2284 2284
Size: 5924
BspHI T'CATG_A
Cuts at: 1130 2489 4356 1130
Size: 1359 1867 2698
Fragments arranged by size:
2698 1867 1359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1611 1903 2163 2262 2974 3188 3490 3871 1611
Size: 292 260 99 712 214 302 381 3664
Fragments arranged by size:
3664 712 381 302 292 260 214 99
BsrBI CCG'CTC
Cuts at: 50 483 834 4216 4270 5754 5919 50
Size: 433 351 3382 54 1484 165 55
Fragments arranged by size:
3382 1484 433 351 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1157 1288 3836 1157
Size: 131 2548 3245
Fragments arranged by size:
3245 2548 131
BsrFI r'CCGG_y
Cuts at: 3922 4103 3922
Size: 181 5743
BssSI C'ACGA_G
Cuts at: 4195 4903 4195
Size: 708 5216
BstAPI GCAn_nnn'nTGC
Cuts at: 2137 2763 3301 2137
Size: 626 538 4760
Fragments arranged by size:
4760 626 538
BstBI TT'CG_AA
Cuts at: 1218 4285 1218
Size: 3067 2857
BstXI CCAn_nnnn'nTGG
Cuts at: 1257 2055 1257
Size: 798 5126
BstYI r'GATC_y
Cuts at: 1 396 507 606 1995 2067 2949 3416 3439
Size: 395 111 99 1389 72 882 467 23
Cuts at: 3439 3774 4020 4326 4338 4424 5476 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1389 1052 882 467 449 395 335 306
246 111 99 86 72 23 12
Bsu36I CC'TnA_GG
Cuts at: 920 1015 1961 2237 920
Size: 95 946 276 4607
Fragments arranged by size:
4607 946 276 95
BtgI C'CryG_G
Cuts at: 233 434 233
Size: 201 5723
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1377 3874 4055 4218 1377
Size: 2497 181 163 3083
Fragments arranged by size:
3083 2497 181 163
BtsI GCAGTG_nn'
Cuts at: 875 1937 2069 2175 3167 875
Size: 1062 132 106 992 3632
Fragments arranged by size:
3632 1062 992 132 106
ClaI AT'CG_AT
Cuts at: 2540 2540
Size: 5924
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 617 652 617
Size: 35 5889
DraI TTT'AAA
Cuts at: 2003 2684 2923 2003
Size: 681 239 5004
Fragments arranged by size:
5004 681 239
DraIII CAC_nnn'GTG
Cuts at: 1403 2221 1403
Size: 818 5106
DrdI GACnn_nn'nnGTC
Cuts at: 2090 3630 4974 2090
Size: 1540 1344 3040
Fragments arranged by size:
3040 1540 1344
EaeI y'GGCC_r
Cuts at: 356 2417 2807 3509 3683 4074 4101 5446 356
Size: 2061 390 702 174 391 27 1345 834
Fragments arranged by size:
2061 1345 834 702 391 390 174 27
EagI C'GGCC_G
Cuts at: 3509 3509
Size: 5924
EarI CTCTTCn'nnn_
Cuts at: 3947 4157 3947
Size: 210 5714
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4132 4874 5020 5734 4132
Size: 742 146 714 4322
Fragments arranged by size:
4322 742 714 146
EcoICRI GAG'CTC
Cuts at: 2866 2936 2866
Size: 70 5854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1537 2500 2538 2641 2800 2875 2891 3749
Size: 567 963 38 103 159 75 16 858
Cuts at: 3749 4181 4534 5196 970
Size: 432 353 662 1698
Fragments arranged by size:
1698 963 858 662 567 432 353 159
103 75 38 16
EcoO109I rG'GnC_Cy
Cuts at: 3063 3063
Size: 5924
EcoRI G'AATT_C
Cuts at: 1868 2928 1868
Size: 1060 4864
EcoRV GAT'ATC
Cuts at: 558 558
Size: 5924
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2002 3943 2002
Size: 1941 3983
FspI TGC'GCA
Cuts at: 3705 3705
Size: 5924
HaeII r_GCGC'y
Cuts at: 1278 1692 2610 3358 3606 4836 5607 1278
Size: 414 918 748 248 1230 771 1595
Fragments arranged by size:
1595 1230 918 771 748 414 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 276 308 769 801 2487 2519 3766 3796 3798
Size: 32 461 32 1686 32 1247 30 2
Cuts at: 3798 3828 276
Size: 30 2372
Fragments arranged by size:
2372 1686 1247 461 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1209 2963 1209
Size: 1754 4170
HindIII A'AGCT_T
Cuts at: 307 1509 307
Size: 1202 4722
KasI G'GCGC_C
Cuts at: 1688 3354 3602 1688
Size: 1666 248 4010
Fragments arranged by size:
4010 1666 248
KpnI G_GTAC'C
Cuts at: 2944 2944
Size: 5924
MfeI C'AATT_G
Cuts at: 1337 1337
Size: 5924
MluI A'CGCG_T
Cuts at: 5357 5357
Size: 5924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1103 4683 4867 1103
Size: 3580 184 2160
Fragments arranged by size:
3580 2160 184
MscI TGG'CCA
Cuts at: 358 2419 3685 358
Size: 2061 1266 2597
Fragments arranged by size:
2597 2061 1266
MslI CAynn'nnrTG
Cuts at: 1255 1543 2053 2317 2494 2684 2856 3913 4040
Size: 288 510 264 177 190 172 1057 127
Cuts at: 4040 1255
Size: 3139
Fragments arranged by size:
3139 1057 510 288 264 190 177 172
127
MspA1I CmG'CkG
Cuts at: 883 2558 3035 3349 3709 4493 4738 883
Size: 1675 477 314 360 784 245 2069
Fragments arranged by size:
2069 1675 784 477 360 314 245
NaeI GCC'GGC
Cuts at: 4105 4105
Size: 5924
NarI GG'CG_CC
Cuts at: 1689 3355 3603 1689
Size: 1666 248 4010
Fragments arranged by size:
4010 1666 248
NcoI C'CATG_G
Cuts at: 434 434
Size: 5924
NgoMIV G'CCGG_C
Cuts at: 4103 4103
Size: 5924
NspI r_CATG'y
Cuts at: 2766 2977 3477 5080 2766
Size: 211 500 1603 3610
Fragments arranged by size:
3610 1603 500 211
PciI A'CATG_T
Cuts at: 5076 5076
Size: 5924
PflMI CCAn_nnn'nTGG
Cuts at: 573 573
Size: 5924
PfoI T'CCnGG_A
Cuts at: 773 1514 773
Size: 741 5183
PmeI GTTT'AAAC
Cuts at: 2923 2923
Size: 5924
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1402 1434 2280 2312 4370 4402 1402
Size: 32 846 32 2058 32 2924
Fragments arranged by size:
2924 2058 846 32 32 32
PshAI GACnn'nnGTC
Cuts at: 2015 2015
Size: 5924
PspOMI G'GGCC_C
Cuts at: 2481 2481
Size: 5924
PspXI vC'TCGA_Gb
Cuts at: 1439 1439
Size: 5924
PstI C_TGCA'G
Cuts at: 1272 2259 2351 2971 3656 1272
Size: 987 92 620 685 3540
Fragments arranged by size:
3540 987 685 620 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5924
PvuII CAG'CTG
Cuts at: 2558 3349 3709 2558
Size: 791 360 4773
Fragments arranged by size:
4773 791 360
RsrII CG'GwC_CG
Cuts at: 4119 4119
Size: 5924
SacI G_AGCT'C
Cuts at: 2868 2938 2868
Size: 70 5854
SalI G'TCGA_C
Cuts at: 2961 2961
Size: 5924
SapI GCTCTTCn'nnn_
Cuts at: 3947 4157 3947
Size: 210 5714
SbfI CC_TGCA'GG
Cuts at: 2971 2971
Size: 5924
ScaI AGT'ACT
Cuts at: 5441 5441
Size: 5924
SexAI A'CCwGG_T
Cuts at: 2217 2217
Size: 5924
SfcI C'TryA_G
Cuts at: 33 1193 1268 2255 2347 2967 3652 4620 4811
Size: 1160 75 987 92 620 685 968 191
Cuts at: 4811 33
Size: 1146
Fragments arranged by size:
1160 1146 987 968 685 620 191 92
75
SfoI GGC'GCC
Cuts at: 1690 3356 3604 1690
Size: 1666 248 4010
Fragments arranged by size:
4010 1666 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5924
SmaI CCC'GGG
Cuts at: 2946 2946
Size: 5924
SmlI C'TyrA_G
Cuts at: 1148 1439 1585 1626 1976 3079 3994 4708 4970
Size: 291 146 41 350 1103 915 714 262
Cuts at: 4970 1148
Size: 2102
Fragments arranged by size:
2102 1103 915 714 350 291 262 146
41
SnaBI TAC'GTA
Cuts at: 2818 2818
Size: 5924
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5924
SphI G_CATG'C
Cuts at: 2977 3477 2977
Size: 500 5424
SspI AAT'ATT
Cuts at: 152 152
Size: 5924
StuI AGG'CCT
Cuts at: 1047 1047
Size: 5924
StyI C'CwwG_G
Cuts at: 419 434 2073 2304 2477 3058 3412 419
Size: 15 1639 231 173 581 354 2931
Fragments arranged by size:
2931 1639 581 354 231 173 15
SwaI ATTT'AAAT
Cuts at: 2684 2684
Size: 5924
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 695 1555 2005 2112 3507 3640 4099 4308 695
Size: 860 450 107 1395 133 459 209 2311
Fragments arranged by size:
2311 1395 860 459 450 209 133 107
TatI w'GTAC_w
Cuts at: 1088 5439 1088
Size: 4351 1573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5441 5473 5441
Size: 32 5892
Tth111I GACn'n_nGTC
Cuts at: 3721 3721
Size: 5924
XbaI T'CTAG_A
Cuts at: 906 2955 906
Size: 2049 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 867 1848 2412 2745 3419 867
Size: 981 564 333 674 3372
Fragments arranged by size:
3372 981 674 564 333
XhoI C'TCGA_G
Cuts at: 1439 1439
Size: 5924
XmaI C'CCGG_G
Cuts at: 2944 2944
Size: 5924
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AgeI AscI BaeI BbvCI BcgI BfrBI
BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I EcoNI
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PsiI PsrI SacII SanDI
SfiI SgrAI SrfI XmnI ZraI