Restriction map for
pF1KB4244
(Circular) (Six-base) MAPSORT of: pF1KB4244.seq Check: 1865 from: 1 to: 3932
pF1KB4244 3932 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 227 227
Size: 3932
AccI GT'mk_AC
Cuts at: 970 970
Size: 3932
Acc65I G'GTAC_C
Cuts at: 948 948
Size: 3932
AclI AA'CG_TT
Cuts at: 3772 3772
Size: 3932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 203 404 539 1757 2189 2936 3204 133
Size: 70 201 135 1218 432 747 268 861
Fragments arranged by size:
1218 861 747 432 268 201 135 70
AflIII A'CryG_T
Cuts at: 1284 2388 3365 1284
Size: 1104 977 1851
Fragments arranged by size:
1851 1104 977
AgeI A'CCGG_T
Cuts at: 2320 2320
Size: 3932
AlwNI CAG_nnn'CTG
Cuts at: 1237 2804 1237
Size: 1567 2365
ApaLI G'TGCA_C
Cuts at: 2702 2702
Size: 3932
ApoI r'AATT_y
Cuts at: 523 533 936 3285 3541 523
Size: 10 403 2349 256 914
Fragments arranged by size:
2349 914 403 256 10
AseI AT'TA_AT
Cuts at: 20 866 20
Size: 846 3086
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
AvaI C'yCGr_G
Cuts at: 283 952 283
Size: 669 3263
BamHI G'GATC_C
Cuts at: 957 957
Size: 3932
BanI G'GyrC_C
Cuts at: 948 1362 1610 1645 948
Size: 414 248 35 3235
Fragments arranged by size:
3235 414 248 35
BanII G_rGCy'C
Cuts at: 496 946 1976 496
Size: 450 1030 2452
Fragments arranged by size:
2452 1030 450
BbeI G_GCGC'C
Cuts at: 1366 1614 1366
Size: 248 3684
BbsI GAAGACnn'nnnn_
Cuts at: 121 121
Size: 3932
BciVI GTATCCnnnnn_n'
Cuts at: 1830 2591 3932 1830
Size: 761 1341 1830
Fragments arranged by size:
1830 1341 761
BclI T'GATC_A
Cuts at: 1452 1452
Size: 3932
BglII A'GATC_T
Cuts at: 1447 3484 1447
Size: 2037 1895
BlpI GC'TnA_GC
Cuts at: 1044 1044
Size: 3932
Bme1580I G_kGCm'C
Cuts at: 1557 1650 2706 1557
Size: 93 1056 2783
Fragments arranged by size:
2783 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 759 1144 1225 1560 3704 759
Size: 385 81 335 2144 987
Fragments arranged by size:
2144 987 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 3932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 752 723
Size: 29 3903
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 1108 1987 2479 2777 411
Size: 697 879 492 298 1566
Fragments arranged by size:
1566 879 697 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3636 3636
Size: 3932
BsaAI yAC'GTr
Cuts at: 1285 1285
Size: 3932
BsaBI GATnn'nnATC
Cuts at: 6 1446 3483 6
Size: 1440 2037 455
Fragments arranged by size:
2037 1440 455
BsaHI Gr'CG_yC
Cuts at: 224 1363 1611 2313 3809 224
Size: 1139 248 702 1496 347
Fragments arranged by size:
1496 1139 702 347 248
BsaWI w'CCGG_w
Cuts at: 1121 1642 2320 2594 2741 3152 1121
Size: 521 678 274 147 411 1901
Fragments arranged by size:
1901 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1357 2115 2692 3188 1357
Size: 758 577 496 2101
Fragments arranged by size:
2101 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1474 1520 2728 104
Size: 1370 46 1208 1308
Fragments arranged by size:
1370 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 946 1724 1914 2706 946
Size: 778 190 792 2172
Fragments arranged by size:
2172 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 496 946 1557 1650 1724 1914 1976 2706 496
Size: 450 611 93 74 190 62 730 1722
Fragments arranged by size:
1722 730 611 450 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3152 3152
Size: 3932
BspHI T'CATG_A
Cuts at: 3108 3108
Size: 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 982 1196 1498 1879 982
Size: 214 302 381 3035
Fragments arranged by size:
3035 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2224 2278 3762 3927 50
Size: 2174 54 1484 165 55
Fragments arranged by size:
2174 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 763 1844 763
Size: 1081 2851
BsrFI r'CCGG_y
Cuts at: 1930 2111 2320 1930
Size: 181 209 3542
Fragments arranged by size:
3542 209 181
BssSI C'ACGA_G
Cuts at: 2203 2561 2203
Size: 358 3574
BstAPI GCAn_nnn'nTGC
Cuts at: 1309 1309
Size: 3932
BstBI TT'CG_AA
Cuts at: 2293 2293
Size: 3932
BstYI r'GATC_y
Cuts at: 1 188 471 544 957 1424 1447 1782 2028
Size: 187 283 73 413 467 23 335 246
Cuts at: 2028 3040 3126 3138 3484 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 413 346 335 283 246
187 86 73 23 12
Bsu36I CC'TnA_GG
Cuts at: 685 685
Size: 3932
BtgI C'CryG_G
Cuts at: 758 758
Size: 3932
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1882 2063 2226 1882
Size: 181 163 3588
Fragments arranged by size:
3588 181 163
BtsI GCAGTG_nn'
Cuts at: 1175 1175
Size: 3932
DraI TTT'AAA
Cuts at: 931 931
Size: 3932
DrdI GACnn_nn'nnGTC
Cuts at: 833 1638 2496 833
Size: 805 858 2269
Fragments arranged by size:
2269 858 805
EaeI y'GGCC_r
Cuts at: 1517 1691 2082 2109 3454 1517
Size: 174 391 27 1345 1995
Fragments arranged by size:
1995 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1517 1517
Size: 3932
EarI CTCTTCn'nnn_
Cuts at: 817 866 1955 2165 817
Size: 49 1089 210 2584
Fragments arranged by size:
2584 1089 210 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2140 2450 2596 3742 2140
Size: 310 146 1146 2330
Fragments arranged by size:
2330 1146 310 146
EcoICRI GAG'CTC
Cuts at: 944 944
Size: 3932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 203 404 539 723 752 1757 2189 2936
Size: 70 201 135 184 29 1005 432 747
Cuts at: 2936 3204 133
Size: 268 861
Fragments arranged by size:
1005 861 747 432 268 201 184 135
70 29
EcoO109I rG'GnC_Cy
Cuts at: 1071 1071
Size: 3932
EcoRI G'AATT_C
Cuts at: 523 936 523
Size: 413 3519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1951 1951
Size: 3932
FspI TGC'GCA
Cuts at: 1713 1713
Size: 3932
HaeII r_GCGC'y
Cuts at: 1366 1614 2636 3615 1366
Size: 248 1022 979 1683
Fragments arranged by size:
1683 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 626 658 1774 1804 1806 1836 626
Size: 32 1116 30 2 30 2722
Fragments arranged by size:
2722 1116 32 30 30 2
HincII GTy'rAC
Cuts at: 971 971
Size: 3932
KasI G'GCGC_C
Cuts at: 1362 1610 1362
Size: 248 3684
KpnI G_GTAC'C
Cuts at: 952 952
Size: 3932
MfeI C'AATT_G
Cuts at: 334 334
Size: 3932
MluI A'CGCG_T
Cuts at: 3365 3365
Size: 3932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 292 2603 2787 292
Size: 2311 184 1437
Fragments arranged by size:
2311 1437 184
MscI TGG'CCA
Cuts at: 1693 1693
Size: 3932
MslI CAynn'nnrTG
Cuts at: 1921 2048 1921
Size: 127 3805
MspA1I CmG'CkG
Cuts at: 153 1043 1357 1717 2730 2975 153
Size: 890 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 890 360 314 245
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 3932
NarI GG'CG_CC
Cuts at: 1363 1611 1363
Size: 248 3684
NcoI C'CATG_G
Cuts at: 758 758
Size: 3932
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 3932
NspI r_CATG'y
Cuts at: 985 1485 2392 985
Size: 500 907 2525
Fragments arranged by size:
2525 907 500
PciI A'CATG_T
Cuts at: 2388 2388
Size: 3932
PmeI GTTT'AAAC
Cuts at: 931 931
Size: 3932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 491 523 3071 3103 491
Size: 32 2548 32 1320
Fragments arranged by size:
2548 1320 32 32
PsiI TTA'TAA
Cuts at: 787 787
Size: 3932
PstI C_TGCA'G
Cuts at: 196 979 1664 196
Size: 783 685 2464
Fragments arranged by size:
2464 783 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3932
PvuII CAG'CTG
Cuts at: 153 1357 1717 153
Size: 1204 360 2368
Fragments arranged by size:
2368 1204 360
RsrII CG'GwC_CG
Cuts at: 2127 2127
Size: 3932
SacI G_AGCT'C
Cuts at: 946 946
Size: 3932
SalI G'TCGA_C
Cuts at: 969 969
Size: 3932
SapI GCTCTTCn'nnn_
Cuts at: 1955 2165 1955
Size: 210 3722
SbfI CC_TGCA'GG
Cuts at: 979 979
Size: 3932
ScaI AGT'ACT
Cuts at: 3449 3449
Size: 3932
SfcI C'TryA_G
Cuts at: 33 192 975 1660 2653 2844 33
Size: 159 783 685 993 191 1121
Fragments arranged by size:
1121 993 783 685 191 159
SfoI GGC'GCC
Cuts at: 1364 1612 1364
Size: 248 3684
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
SmaI CCC'GGG
Cuts at: 285 954 285
Size: 669 3263
SmlI C'TyrA_G
Cuts at: 390 1087 2002 2494 2756 390
Size: 697 915 492 262 1566
Fragments arranged by size:
1566 915 697 492 262
SpeI A'CTAG_T
Cuts at: 67 586 67
Size: 519 3413
SphI G_CATG'C
Cuts at: 985 1485 985
Size: 500 3432
SspI AAT'ATT
Cuts at: 378 378
Size: 3932
StyI C'CwwG_G
Cuts at: 758 1066 1420 758
Size: 308 354 3270
Fragments arranged by size:
3270 354 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1515 1648 2107 2316 1515
Size: 133 459 209 3131
Fragments arranged by size:
3131 459 209 133
TatI w'GTAC_w
Cuts at: 330 3447 330
Size: 3117 815
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 3900
Tth111I GACn'n_nGTC
Cuts at: 1729 1729
Size: 3932
XbaI T'CTAG_A
Cuts at: 963 963
Size: 3932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 308 1427 308
Size: 1119 2813
XmaI C'CCGG_G
Cuts at: 283 952 283
Size: 669 3263
ZraI GAC'GTC
Cuts at: 225 225
Size: 3932
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI