Restriction map for
pF1KB4223
(Circular) (Six-base) MAPSORT of: pF1KB4223.seq Check: 1796 from: 1 to: 4361
pF1KB4223 4361 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 1151 1151
Size: 4361
AccI GT'mk_AC
Cuts at: 1399 1399
Size: 4361
Acc65I G'GTAC_C
Cuts at: 1377 1377
Size: 4361
AclI AA'CG_TT
Cuts at: 4201 4201
Size: 4361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 949 2186 2618 3365 3633 919
Size: 30 1237 432 747 268 1647
Fragments arranged by size:
1647 1237 747 432 268 30
AflIII A'CryG_T
Cuts at: 517 716 1713 2817 3794 517
Size: 199 997 1104 977 1084
Fragments arranged by size:
1104 1084 997 977 199
AgeI A'CCGG_T
Cuts at: 2749 2749
Size: 4361
AleI CACnn'nnGTG
Cuts at: 136 136
Size: 4361
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 898 930 898
Size: 32 4329
AlwNI CAG_nnn'CTG
Cuts at: 657 855 1666 3233 657
Size: 198 811 1567 1785
Fragments arranged by size:
1785 1567 811 198
ApaLI G'TGCA_C
Cuts at: 3131 3131
Size: 4361
ApoI r'AATT_y
Cuts at: 462 1129 1365 3714 3970 462
Size: 667 236 2349 256 853
Fragments arranged by size:
2349 853 667 256 236
AseI AT'TA_AT
Cuts at: 20 20
Size: 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
AvaI C'yCGr_G
Cuts at: 1381 1381
Size: 4361
BamHI G'GATC_C
Cuts at: 1386 1386
Size: 4361
BanI G'GyrC_C
Cuts at: 147 1377 1791 2039 2074 147
Size: 1230 414 248 35 2434
Fragments arranged by size:
2434 1230 414 248 35
BanII G_rGCy'C
Cuts at: 1375 2405 1375
Size: 1030 3331
BbeI G_GCGC'C
Cuts at: 151 1795 2043 151
Size: 1644 248 2469
Fragments arranged by size:
2469 1644 248
BbvCI CC'TCA_GC
Cuts at: 235 1099 235
Size: 864 3497
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3020 4361 2259
Size: 761 1341 2259
Fragments arranged by size:
2259 1341 761
BclI T'GATC_A
Cuts at: 447 1881 447
Size: 1434 2927
BglII A'GATC_T
Cuts at: 1289 1876 3913 1289
Size: 587 2037 1737
Fragments arranged by size:
2037 1737 587
BlpI GC'TnA_GC
Cuts at: 154 277 1473 154
Size: 123 1196 3042
Fragments arranged by size:
3042 1196 123
Bme1580I G_kGCm'C
Cuts at: 1986 2079 3135 1986
Size: 93 1056 3212
Fragments arranged by size:
3212 1056 93
BmgBI CAC'GTC
Cuts at: 576 969 576
Size: 393 3968
BmrI ACTGGGnnnn_n'
Cuts at: 1573 1654 1989 4133 1573
Size: 81 335 2144 1801
Fragments arranged by size:
2144 1801 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1332 2728 1332
Size: 1396 2965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 910
Size: 4361
Bpu10I CC'TnA_GC
Cuts at: 235 1099 1307 235
Size: 864 208 3289
Fragments arranged by size:
3289 864 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1537 2416 2908 3206 742
Size: 795 879 492 298 1897
Fragments arranged by size:
1897 879 795 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4065 4065
Size: 4361
BsaAI yAC'GTr
Cuts at: 1714 1714
Size: 4361
BsaBI GATnn'nnATC
Cuts at: 6 1875 3912 6
Size: 1869 2037 455
Fragments arranged by size:
2037 1869 455
BsaHI Gr'CG_yC
Cuts at: 148 1148 1792 2040 2742 4238 148
Size: 1000 644 248 702 1496 271
Fragments arranged by size:
1496 1000 702 644 271 248
BsaWI w'CCGG_w
Cuts at: 1077 1550 2071 2749 3023 3170 3581 1077
Size: 473 521 678 274 147 411 1857
Fragments arranged by size:
1857 678 521 473 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 883 1003 1018 1036 1087 883
Size: 120 15 18 51 4157
Fragments arranged by size:
4157 120 51 18 15
BseYI C'CCAG_C
Cuts at: 376 1786 2544 3121 3617 376
Size: 1410 758 577 496 1120
Fragments arranged by size:
1410 1120 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 1232 105
Size: 1127 3234
BsiEI CG_ry'CG
Cuts at: 104 540 1903 1949 3157 104
Size: 436 1363 46 1208 1308
Fragments arranged by size:
1363 1308 1208 436 46
BsiHKAI G_wGCw'C
Cuts at: 1375 2153 2343 3135 1375
Size: 778 190 792 2601
Fragments arranged by size:
2601 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1375 1986 2079 2153 2343 2405 3135 1375
Size: 611 93 74 190 62 730 2601
Fragments arranged by size:
2601 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1077 3581 1077
Size: 2504 1857
BspHI T'CATG_A
Cuts at: 3537 3537
Size: 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 380 1411 1625 1927 2308 380
Size: 1031 214 302 381 2433
Fragments arranged by size:
2433 1031 381 302 214
BsrBI CCG'CTC
Cuts at: 50 153 1008 2653 2707 4191 4356 50
Size: 103 855 1645 54 1484 165 55
Fragments arranged by size:
1645 1484 855 165 103 55 54
BsrDI GCAATG_nn'
Cuts at: 288 2273 288
Size: 1985 2376
BsrFI r'CCGG_y
Cuts at: 539 2359 2540 2749 539
Size: 1820 181 209 2151
Fragments arranged by size:
2151 1820 209 181
BssSI C'ACGA_G
Cuts at: 2632 2990 2632
Size: 358 4003
BstAPI GCAn_nnn'nTGC
Cuts at: 855 1255 1738 855
Size: 400 483 3478
Fragments arranged by size:
3478 483 400
BstBI TT'CG_AA
Cuts at: 2722 2722
Size: 4361
BstEII G'GTnAC_C
Cuts at: 705 705
Size: 4361
BstXI CCAn_nnnn'nTGG
Cuts at: 138 138
Size: 4361
BstYI r'GATC_y
Cuts at: 1 1289 1386 1853 1876 2211 2457 3469 3555
Size: 1288 97 467 23 335 246 1012 86
Cuts at: 3555 3567 3913 1
Size: 12 346 449
Fragments arranged by size:
1288 1012 467 449 346 335 246 97
86 23 12
BtgI C'CryG_G
Cuts at: 131 407 677 131
Size: 276 270 3815
Fragments arranged by size:
3815 276 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 727 2311 2492 2655 727
Size: 1584 181 163 2433
Fragments arranged by size:
2433 1584 181 163
BtsI GCAGTG_nn'
Cuts at: 1604 1604
Size: 4361
DraI TTT'AAA
Cuts at: 1360 1360
Size: 4361
DraIII CAC_nnn'GTG
Cuts at: 969 969
Size: 4361
DrdI GACnn_nn'nnGTC
Cuts at: 2067 2925 2067
Size: 858 3503
EaeI y'GGCC_r
Cuts at: 1202 1946 2120 2511 2538 3883 1202
Size: 744 174 391 27 1345 1680
Fragments arranged by size:
1680 1345 744 391 174 27
EagI C'GGCC_G
Cuts at: 1946 1946
Size: 4361
EarI CTCTTCn'nnn_
Cuts at: 361 2384 2594 361
Size: 2023 210 2128
Fragments arranged by size:
2128 2023 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 595 2569 2879 3025 4171 595
Size: 1974 310 146 1146 785
Fragments arranged by size:
1974 1146 785 310 146
EcoICRI GAG'CTC
Cuts at: 1373 1373
Size: 4361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 919 949 2186 2618 3365 3633 910
Size: 9 30 1237 432 747 268 1638
Fragments arranged by size:
1638 1237 747 432 268 30 9
EcoO109I rG'GnC_Cy
Cuts at: 1500 1500
Size: 4361
EcoRI G'AATT_C
Cuts at: 462 1129 1365 462
Size: 667 236 3458
Fragments arranged by size:
3458 667 236
EcoRV GAT'ATC
Cuts at: 1215 1215
Size: 4361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4361
FspI TGC'GCA
Cuts at: 2142 2142
Size: 4361
HaeII r_GCGC'y
Cuts at: 151 161 482 546 1795 2043 3065 4044 151
Size: 10 321 64 1249 248 1022 979 468
Fragments arranged by size:
1249 1022 979 468 321 248 64 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1324 1356 2203 2233 2235 2265 1324
Size: 32 847 30 2 30 3420
Fragments arranged by size:
3420 847 32 30 30 2
HincII GTy'rAC
Cuts at: 1146 1400 1146
Size: 254 4107
KasI G'GCGC_C
Cuts at: 147 1791 2039 147
Size: 1644 248 2469
Fragments arranged by size:
2469 1644 248
KpnI G_GTAC'C
Cuts at: 1381 1381
Size: 4361
MluI A'CGCG_T
Cuts at: 517 3794 517
Size: 3277 1084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 1367 3032 3216 140
Size: 1227 1665 184 1285
Fragments arranged by size:
1665 1285 1227 184
MscI TGG'CCA
Cuts at: 1204 2122 1204
Size: 918 3443
MslI CAynn'nnrTG
Cuts at: 136 304 2350 2477 136
Size: 168 2046 127 2020
Fragments arranged by size:
2046 2020 168 127
MspA1I CmG'CkG
Cuts at: 927 1472 1786 2146 3159 3404 927
Size: 545 314 360 1013 245 1884
Fragments arranged by size:
1884 1013 545 360 314 245
NaeI GCC'GGC
Cuts at: 2542 2542
Size: 4361
NarI GG'CG_CC
Cuts at: 148 1792 2040 148
Size: 1644 248 2469
Fragments arranged by size:
2469 1644 248
NcoI C'CATG_G
Cuts at: 407 407
Size: 4361
NgoMIV G'CCGG_C
Cuts at: 2540 2540
Size: 4361
NspI r_CATG'y
Cuts at: 666 862 1255 1414 1914 2821 666
Size: 196 393 159 500 907 2206
Fragments arranged by size:
2206 907 500 393 196 159
PciI A'CATG_T
Cuts at: 2817 2817
Size: 4361
PflMI CCAn_nnn'nTGG
Cuts at: 969 969
Size: 4361
PfoI T'CCnGG_A
Cuts at: 887 887
Size: 4361
PmeI GTTT'AAAC
Cuts at: 1360 1360
Size: 4361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 4329
PstI C_TGCA'G
Cuts at: 1408 2093 1408
Size: 685 3676
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4361
PvuII CAG'CTG
Cuts at: 927 1786 2146 927
Size: 859 360 3142
Fragments arranged by size:
3142 859 360
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 4361
SacI G_AGCT'C
Cuts at: 1375 1375
Size: 4361
SalI G'TCGA_C
Cuts at: 1398 1398
Size: 4361
SapI GCTCTTCn'nnn_
Cuts at: 2384 2594 2384
Size: 210 4151
SbfI CC_TGCA'GG
Cuts at: 1408 1408
Size: 4361
ScaI AGT'ACT
Cuts at: 1315 3878 1315
Size: 2563 1798
SfcI C'TryA_G
Cuts at: 33 327 942 1404 2089 3082 3273 33
Size: 294 615 462 685 993 191 1121
Fragments arranged by size:
1121 993 685 615 462 294 191
SfoI GGC'GCC
Cuts at: 149 1793 2041 149
Size: 1644 248 2469
Fragments arranged by size:
2469 1644 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
SmaI CCC'GGG
Cuts at: 1383 1383
Size: 4361
SmlI C'TyrA_G
Cuts at: 757 1516 2431 2923 3185 757
Size: 759 915 492 262 1933
Fragments arranged by size:
1933 915 759 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4361
SphI G_CATG'C
Cuts at: 1414 1914 1414
Size: 500 3861
StyI C'CwwG_G
Cuts at: 407 803 1495 1849 407
Size: 396 692 354 2919
Fragments arranged by size:
2919 692 396 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1033 1944 2077 2536 2745 1033
Size: 911 133 459 209 2649
Fragments arranged by size:
2649 911 459 209 133
TatI w'GTAC_w
Cuts at: 1313 3876 1313
Size: 2563 1798
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1009 1041 3878 3910 1009
Size: 32 2837 32 1460
Fragments arranged by size:
2837 1460 32 32
Tth111I GACn'n_nGTC
Cuts at: 561 2158 561
Size: 1597 2764
XbaI T'CTAG_A
Cuts at: 1392 1392
Size: 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 508 573 1856 508
Size: 65 1283 3013
Fragments arranged by size:
3013 1283 65
XmaI C'CCGG_G
Cuts at: 1381 1381
Size: 4361
XmnI GAAnn'nnTTC
Cuts at: 187 211 718 187
Size: 24 507 3830
Fragments arranged by size:
3830 507 24
ZraI GAC'GTC
Cuts at: 1149 1149
Size: 4361
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI