Restriction map for
pF1KB4196
(Circular) (Six-base) MAPSORT of: pF1KB4196.seq Check: 8230 from: 1 to: 5984
pF1KB4196 5984 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 217 217
Size: 5984
AccI GT'mk_AC
Cuts at: 3022 3022
Size: 5984
Acc65I G'GTAC_C
Cuts at: 1973 3000 1973
Size: 1027 4957
AclI AA'CG_TT
Cuts at: 1476 1997 5824 1476
Size: 521 3827 1636
Fragments arranged by size:
3827 1636 521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1627 1638 2005 2545 3809 4241 4594 5256
Size: 1215 11 367 540 1264 432 353 662
Cuts at: 5256 412
Size: 1140
Fragments arranged by size:
1264 1215 1140 662 540 432 367 353
11
AflII C'TTAA_G
Cuts at: 1102 1102
Size: 5984
AflIII A'CryG_T
Cuts at: 3336 5136 5417 3336
Size: 1800 281 3903
Fragments arranged by size:
3903 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2334 2334
Size: 5984
AleI CACnn'nnGTG
Cuts at: 1627 1700 1627
Size: 73 5911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2068 2068
Size: 5984
AlwNI CAG_nnn'CTG
Cuts at: 1047 1842 2370 2715 3289 4727 1047
Size: 795 528 345 574 1438 2304
Fragments arranged by size:
2304 1438 795 574 528 345
ApaI G_GGCC'C
Cuts at: 577 577
Size: 5984
ApaLI G'TGCA_C
Cuts at: 1018 4822 1018
Size: 3804 2180
ApoI r'AATT_y
Cuts at: 949 1035 2134 2988 5337 5593 949
Size: 86 1099 854 2349 256 1340
Fragments arranged by size:
2349 1340 1099 854 256 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 5984
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5984
AvaI C'yCGr_G
Cuts at: 569 2816 3004 569
Size: 2247 188 3549
Fragments arranged by size:
3549 2247 188
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1630 1663 1965 1998 1630
Size: 33 302 33 5616
Fragments arranged by size:
5616 302 33 33
BamHI G'GATC_C
Cuts at: 686 3009 686
Size: 2323 3661
BanI G'GyrC_C
Cuts at: 313 1728 1973 2519 2553 3000 3414 3662 3697
Size: 1415 245 546 34 447 414 248 35
Cuts at: 3697 313
Size: 2600
Fragments arranged by size:
2600 1415 546 447 414 248 245 35
34
BanII G_rGCy'C
Cuts at: 577 884 1781 1865 2998 4028 577
Size: 307 897 84 1133 1030 2533
Fragments arranged by size:
2533 1133 1030 897 307 84
BbeI G_GCGC'C
Cuts at: 2523 3418 3666 2523
Size: 895 248 4841
Fragments arranged by size:
4841 895 248
BbvCI CC'TCA_GC
Cuts at: 857 2168 2515 2549 857
Size: 1311 347 34 4292
Fragments arranged by size:
4292 1311 347 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 191 225 90
Size: 34 67 34 5849
Fragments arranged by size:
5849 67 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2638 3882 4938 5984 2638
Size: 1244 1056 1046 2638
Fragments arranged by size:
2638 1244 1056 1046
BclI T'GATC_A
Cuts at: 3504 3504
Size: 5984
BglII A'GATC_T
Cuts at: 3499 5536 3499
Size: 2037 3947
BlpI GC'TnA_GC
Cuts at: 460 488 1910 3096 460
Size: 28 1422 1186 3348
Fragments arranged by size:
3348 1422 1186 28
Bme1580I G_kGCm'C
Cuts at: 577 1022 1733 1957 3609 3702 4826 577
Size: 445 711 224 1652 93 1124 1735
Fragments arranged by size:
1735 1652 1124 711 445 224 93
BmrI ACTGGGnnnn_n'
Cuts at: 1775 1830 1860 2552 2927 3196 3277 3612 5756
Size: 55 30 692 375 269 81 335 2144
Cuts at: 5756 1775
Size: 2003
Fragments arranged by size:
2144 2003 692 375 335 269 81 55
30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 423 4351 423
Size: 3928 2056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 817 1096 1864 1879 2221 2367 2488 2674
Size: 201 279 768 15 342 146 121 186
Cuts at: 2674 2822 616
Size: 148 3778
Fragments arranged by size:
3778 768 342 279 201 186 148 146
121 15
Bpu10I CC'TnA_GC
Cuts at: 283 605 857 2168 2515 2549 283
Size: 322 252 1311 347 34 3718
Fragments arranged by size:
3718 1311 347 322 252 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 3160 4039 4753 5051 242
Size: 2918 879 714 298 1175
Fragments arranged by size:
2918 1175 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5688 5688
Size: 5984
BsaAI yAC'GTr
Cuts at: 1428 3337 1428
Size: 1909 4075
BsaBI GATnn'nnATC
Cuts at: 6 3498 5535 6
Size: 3492 2037 455
Fragments arranged by size:
3492 2037 455
BsaHI Gr'CG_yC
Cuts at: 1084 2221 2520 3415 3663 4365 5861 1084
Size: 1137 299 895 248 702 1496 1207
Fragments arranged by size:
1496 1207 1137 895 702 299 248
BsaWI w'CCGG_w
Cuts at: 1976 2495 3173 3694 4372 4783 4930 5204 1976
Size: 519 678 521 678 411 147 274 2756
Fragments arranged by size:
2756 678 678 521 519 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1141 1171 1852 1882 2245 2275 1141
Size: 30 681 30 363 30 4850
Fragments arranged by size:
4850 681 363 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 245 490 649 658 1149 1152 1498 1534 2554
Size: 245 159 9 491 3 346 36 1020
Cuts at: 2554 2620 245
Size: 66 3609
Fragments arranged by size:
3609 1020 491 346 245 159 66 36
9 3
BseYI C'CCAG_C
Cuts at: 268 620 1244 1740 2702 3409 4167 4832 5240
Size: 352 624 496 962 707 758 665 408
Cuts at: 5240 268
Size: 1012
Fragments arranged by size:
1012 962 758 707 665 624 496 408
352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 2038 233
Size: 1805 4179
BsiEI CG_ry'CG
Cuts at: 104 3526 3572 4802 104
Size: 3422 46 1230 1286
Fragments arranged by size:
3422 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 920 1022 1427 1939 2998 3776 3966 4826 920
Size: 102 405 512 1059 778 190 860 2078
Fragments arranged by size:
2078 1059 860 778 512 405 190 102
BsmBI CGTCTCn'nnnn_
Cuts at: 2212 2650 2212
Size: 438 5546
Bsp1286I G_dGCh'C
Cuts at: 577 884 920 1022 1427 1733 1781 1865 1939
Size: 307 36 102 405 306 48 84 74
Cuts at: 1939 1957 2998 3609 3702 3776 3966 4028 4826
Size: 18 1041 611 93 74 190 62 798
Cuts at: 4826 577
Size: 1735
Fragments arranged by size:
1735 1041 798 611 405 307 306 190
102 93 84 74 74 62 48 36
18
BspHI T'CATG_A
Cuts at: 156 1713 4416 156
Size: 1557 2703 1724
Fragments arranged by size:
2703 1724 1557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 217 2023 2378 3034 3248 3550 3931 217
Size: 1806 355 656 214 302 381 2270
Fragments arranged by size:
2270 1806 656 381 355 302 214
BsrBI CCG'CTC
Cuts at: 50 789 2141 4276 4330 5814 5979 50
Size: 739 1352 2135 54 1484 165 55
Fragments arranged by size:
2135 1484 1352 739 165 55 54
BsrDI GCAATG_nn'
Cuts at: 830 1604 1776 1904 2607 3896 830
Size: 774 172 128 703 1289 2918
Fragments arranged by size:
2918 1289 774 703 172 128
BsrFI r'CCGG_y
Cuts at: 2223 3982 4163 2223
Size: 1759 181 4044
Fragments arranged by size:
4044 1759 181
BssSI C'ACGA_G
Cuts at: 4255 4963 4255
Size: 708 5276
BstAPI GCAn_nnn'nTGC
Cuts at: 1410 1610 2196 3361 1410
Size: 200 586 1165 4033
Fragments arranged by size:
4033 1165 586 200
BstBI TT'CG_AA
Cuts at: 4345 4345
Size: 5984
BstEII G'GTnAC_C
Cuts at: 1899 1899
Size: 5984
BstXI CCAn_nnnn'nTGG
Cuts at: 1386 1386
Size: 5984
BstYI r'GATC_y
Cuts at: 1 686 891 2468 2669 3009 3476 3499 3834
Size: 685 205 1577 201 340 467 23 335
Cuts at: 3834 4080 4386 4398 4484 5536 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1577 1052 685 467 449 340 335 306
246 205 201 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 221 221
Size: 5984
BtgI C'CryG_G
Cuts at: 1871 1871
Size: 5984
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3934 4115 4278 3934
Size: 181 163 5640
Fragments arranged by size:
5640 181 163
BtsI GCAGTG_nn'
Cuts at: 1319 1452 1957 2439 2763 3227 1319
Size: 133 505 482 324 464 4076
Fragments arranged by size:
4076 505 482 464 324 133
DraI TTT'AAA
Cuts at: 2983 2983
Size: 5984
DraIII CAC_nnn'GTG
Cuts at: 1519 1519
Size: 5984
DrdI GACnn_nn'nnGTC
Cuts at: 3690 5034 3690
Size: 1344 4640
EaeI y'GGCC_r
Cuts at: 728 1664 2242 2438 2615 3569 3743 4134 4161
Size: 936 578 196 177 954 174 391 27
Cuts at: 4161 5506 728
Size: 1345 1206
Fragments arranged by size:
1345 1206 954 936 578 391 196 177
174 27
EagI C'GGCC_G
Cuts at: 3569 3569
Size: 5984
EarI CTCTTCn'nnn_
Cuts at: 443 901 1657 4007 4217 443
Size: 458 756 2350 210 2210
Fragments arranged by size:
2350 2210 756 458 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4192 4934 5080 5794 4192
Size: 742 146 714 4382
Fragments arranged by size:
4382 742 714 146
EcoICRI GAG'CTC
Cuts at: 2996 2996
Size: 5984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 616 817 1096 1627 1638 1864 1879 2005
Size: 204 201 279 531 11 226 15 126
Cuts at: 2005 2221 2367 2488 2545 2674 2822 3809 4241
Size: 216 146 121 57 129 148 987 432
Cuts at: 4241 4594 5256 412
Size: 353 662 1140
Fragments arranged by size:
1140 987 662 531 432 353 279 226
216 204 201 148 146 129 126 121
57 15 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2171 2171
Size: 5984
EcoO109I rG'GnC_Cy
Cuts at: 573 574 1044 3123 573
Size: 1 470 2079 3434
Fragments arranged by size:
3434 2079 470 1
EcoRI G'AATT_C
Cuts at: 949 1035 2988 949
Size: 86 1953 3945
Fragments arranged by size:
3945 1953 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4003 4003
Size: 5984
FspI TGC'GCA
Cuts at: 3765 3765
Size: 5984
HaeII r_GCGC'y
Cuts at: 1695 2523 3418 3666 4896 5667 1695
Size: 828 895 248 1230 771 2012
Fragments arranged by size:
2012 1230 895 828 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 221 253 466 498 678 710 1852 1884 2245
Size: 32 213 32 180 32 1142 32 361
Cuts at: 2245 2277 2289 2320 2321 2352 2851 2883 3826
Size: 32 12 31 1 31 499 32 943
Cuts at: 3826 3856 3858 3888 221
Size: 30 2 30 2317
Fragments arranged by size:
2317 1142 943 499 361 213 180 32
32 32 32 32 32 31 31 30
30 12 2 1
HincII GTy'rAC
Cuts at: 752 1506 1854 2001 3023 752
Size: 754 348 147 1022 3713
Fragments arranged by size:
3713 1022 754 348 147
HindIII A'AGCT_T
Cuts at: 424 424
Size: 5984
KasI G'GCGC_C
Cuts at: 2519 3414 3662 2519
Size: 895 248 4841
Fragments arranged by size:
4841 895 248
KpnI G_GTAC'C
Cuts at: 1977 3004 1977
Size: 1027 4957
MfeI C'AATT_G
Cuts at: 1536 1536
Size: 5984
MluI A'CGCG_T
Cuts at: 5417 5417
Size: 5984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 334 4743 4927 334
Size: 4409 184 1391
Fragments arranged by size:
4409 1391 184
MscI TGG'CCA
Cuts at: 730 1666 2244 2440 3745 730
Size: 936 578 196 1305 2969
Fragments arranged by size:
2969 1305 936 578 196
MslI CAynn'nnrTG
Cuts at: 1324 1614 1627 1700 1726 2600 3973 4100 1324
Size: 290 13 73 26 874 1373 127 3208
Fragments arranged by size:
3208 1373 874 290 127 73 26 13
MspA1I CmG'CkG
Cuts at: 172 492 684 761 1571 1740 2548 2706 3095
Size: 320 192 77 810 169 808 158 389
Cuts at: 3095 3409 3769 4553 4798 172
Size: 314 360 784 245 1358
Fragments arranged by size:
1358 810 808 784 389 360 320 314
245 192 169 158 77
NaeI GCC'GGC
Cuts at: 2225 4165 2225
Size: 1940 4044
NarI GG'CG_CC
Cuts at: 2520 3415 3663 2520
Size: 895 248 4841
Fragments arranged by size:
4841 895 248
NcoI C'CATG_G
Cuts at: 1871 1871
Size: 5984
NgoMIV G'CCGG_C
Cuts at: 2223 4163 2223
Size: 1940 4044
NspI r_CATG'y
Cuts at: 544 1244 1613 2667 3037 3537 5140 544
Size: 700 369 1054 370 500 1603 1388
Fragments arranged by size:
1603 1388 1054 700 500 370 369
PasI CC'CwG_GG
Cuts at: 1178 1178
Size: 5984
PciI A'CATG_T
Cuts at: 5136 5136
Size: 5984
PflMI CCAn_nnn'nTGG
Cuts at: 1047 1244 1842 1872 1877 2413 1047
Size: 197 598 30 5 536 4618
Fragments arranged by size:
4618 598 536 197 30 5
PfoI T'CCnGG_A
Cuts at: 1112 1841 1112
Size: 729 5255
PmeI GTTT'AAAC
Cuts at: 2983 2983
Size: 5984
PmlI CAC'GTG
Cuts at: 1428 1428
Size: 5984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4430 4462 4430
Size: 32 5952
PspOMI G'GGCC_C
Cuts at: 573 573
Size: 5984
PstI C_TGCA'G
Cuts at: 1415 2711 2735 2769 2856 3031 3716 1415
Size: 1296 24 34 87 175 685 3683
Fragments arranged by size:
3683 1296 685 175 87 34 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5984
PvuII CAG'CTG
Cuts at: 172 492 1740 2548 2706 3409 3769 172
Size: 320 1248 808 158 703 360 2387
Fragments arranged by size:
2387 1248 808 703 360 320 158
RsrII CG'GwC_CG
Cuts at: 4179 4179
Size: 5984
SacI G_AGCT'C
Cuts at: 2998 2998
Size: 5984
SalI G'TCGA_C
Cuts at: 3021 3021
Size: 5984
SapI GCTCTTCn'nnn_
Cuts at: 4007 4217 4007
Size: 210 5774
SbfI CC_TGCA'GG
Cuts at: 3031 3031
Size: 5984
ScaI AGT'ACT
Cuts at: 5501 5501
Size: 5984
SexAI A'CCwGG_T
Cuts at: 1364 1364
Size: 5984
SfcI C'TryA_G
Cuts at: 33 499 1078 1411 1944 2211 2461 2707 2731
Size: 466 579 333 533 267 250 246 24
Cuts at: 2731 2765 2852 3027 3712 4680 4871 33
Size: 34 87 175 685 968 191 1146
Fragments arranged by size:
1146 968 685 579 533 466 333 267
250 246 191 175 87 34 24
SfoI GGC'GCC
Cuts at: 2521 3416 3664 2521
Size: 895 248 4841
Fragments arranged by size:
4841 895 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5984
SmaI CCC'GGG
Cuts at: 2818 3006 2818
Size: 188 5796
SmlI C'TyrA_G
Cuts at: 257 1102 3139 4054 4768 5030 257
Size: 845 2037 915 714 262 1211
Fragments arranged by size:
2037 1211 915 845 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5984
SphI G_CATG'C
Cuts at: 1613 2667 3037 3537 1613
Size: 1054 370 500 4060
Fragments arranged by size:
4060 1054 500 370
StuI AGG'CCT
Cuts at: 403 610 403
Size: 207 5777
StyI C'CwwG_G
Cuts at: 1698 1871 3118 3472 1698
Size: 173 1247 354 4210
Fragments arranged by size:
4210 1247 354 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 641 1212 1732 2571 2754 3567 3700 4159 4368
Size: 571 520 839 183 813 133 459 209
Cuts at: 4368 641
Size: 2257
Fragments arranged by size:
2257 839 813 571 520 459 209 183
133
TatI w'GTAC_w
Cuts at: 5499 5499
Size: 5984
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1141 1173 5501 5533 1141
Size: 32 4328 32 1592
Fragments arranged by size:
4328 1592 32 32
Tth111I GACn'n_nGTC
Cuts at: 3781 3781
Size: 5984
XbaI T'CTAG_A
Cuts at: 3015 3015
Size: 5984
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1129 1696 1878 2967 3479 114
Size: 1015 567 182 1089 512 2619
Fragments arranged by size:
2619 1089 1015 567 512 182
XmaI C'CCGG_G
Cuts at: 2816 3004 2816
Size: 188 5796
XmnI GAAnn'nnTTC
Cuts at: 1640 1990 2134 1640
Size: 350 144 5490
Fragments arranged by size:
5490 350 144
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AloI AscI AvrII BbsI BfrBI
BglI BmgBI BmtI BsiWI BsmI BspEI BsrGI BssHII
BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI