Restriction map for
pF1KB4182
(Circular) (Six-base) MAPSORT of: pF1KB4182.seq Check: 1285 from: 1 to: 4181
pF1KB4182 4181 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 770 770
Size: 4181
AccI GT'mk_AC
Cuts at: 1219 1219
Size: 4181
Acc65I G'GTAC_C
Cuts at: 1197 1197
Size: 4181
AclI AA'CG_TT
Cuts at: 4021 4021
Size: 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 2006 2438 3185 3453 727
Size: 1279 432 747 268 1455
Fragments arranged by size:
1455 1279 747 432 268
AflIII A'CryG_T
Cuts at: 1533 2637 3614 1533
Size: 1104 977 2100
Fragments arranged by size:
2100 1104 977
AgeI A'CCGG_T
Cuts at: 2569 2569
Size: 4181
AhdI GACnn_n'nnGTC
Cuts at: 294 294
Size: 4181
AlwNI CAG_nnn'CTG
Cuts at: 1486 3053 1486
Size: 1567 2614
ApaI G_GGCC'C
Cuts at: 752 1101 752
Size: 349 3832
ApaLI G'TGCA_C
Cuts at: 142 2951 142
Size: 2809 1372
ApoI r'AATT_y
Cuts at: 1185 3534 3790 1185
Size: 2349 256 1576
Fragments arranged by size:
2349 1576 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
AvaI C'yCGr_G
Cuts at: 161 802 1100 1201 161
Size: 641 298 101 3141
Fragments arranged by size:
3141 641 298 101
BamHI G'GATC_C
Cuts at: 1206 1206
Size: 4181
BanI G'GyrC_C
Cuts at: 499 838 847 1197 1611 1859 1894 499
Size: 339 9 350 414 248 35 2786
Fragments arranged by size:
2786 414 350 339 248 35 9
BanII G_rGCy'C
Cuts at: 287 421 752 1101 1195 2225 287
Size: 134 331 349 94 1030 2243
Fragments arranged by size:
2243 1030 349 331 134 94
BbeI G_GCGC'C
Cuts at: 842 1615 1863 842
Size: 773 248 3160
Fragments arranged by size:
3160 773 248
BbvCI CC'TCA_GC
Cuts at: 772 772
Size: 4181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4147
BciVI GTATCCnnnnn_n'
Cuts at: 2079 2840 4181 2079
Size: 761 1341 2079
Fragments arranged by size:
2079 1341 761
BclI T'GATC_A
Cuts at: 185 1701 185
Size: 1516 2665
BglI GCCn_nnn'nGGC
Cuts at: 880 880
Size: 4181
BglII A'GATC_T
Cuts at: 672 1696 3733 672
Size: 1024 2037 1120
Fragments arranged by size:
2037 1120 1024
BlpI GC'TnA_GC
Cuts at: 990 1293 990
Size: 303 3878
Bme1580I G_kGCm'C
Cuts at: 146 502 583 599 689 752 1101 1806 1899
Size: 356 81 16 90 63 349 705 93
Cuts at: 1899 2955 146
Size: 1056 1372
Fragments arranged by size:
1372 1056 705 356 349 93 90 81
63 16
BmrI ACTGGGnnnn_n'
Cuts at: 1393 1474 1809 3953 1393
Size: 81 335 2144 1621
Fragments arranged by size:
2144 1621 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 187 2548 187
Size: 2361 1820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1023 1081 970
Size: 53 58 4070
Fragments arranged by size:
4070 58 53
Bpu10I CC'TnA_GC
Cuts at: 772 772
Size: 4181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 1098 1357 2236 2728 3026 103
Size: 995 259 879 492 298 1258
Fragments arranged by size:
1258 995 879 492 298 259
BsaI GGTCTCn'nnnn_
Cuts at: 3885 3885
Size: 4181
BsaAI yAC'GTr
Cuts at: 698 1534 698
Size: 836 3345
BsaBI GATnn'nnATC
Cuts at: 6 1695 3732 6
Size: 1689 2037 455
Fragments arranged by size:
2037 1689 455
BsaHI Gr'CG_yC
Cuts at: 242 767 839 1612 1860 2562 4058 242
Size: 525 72 773 248 702 1496 365
Fragments arranged by size:
1496 773 702 525 365 248 72
BsaWI w'CCGG_w
Cuts at: 1370 1891 2569 2843 2990 3401 1370
Size: 521 678 274 147 411 2150
Fragments arranged by size:
2150 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 323 353 846 876 913 943 1015 1045 323
Size: 30 493 30 37 30 72 30 3459
Fragments arranged by size:
3459 493 72 37 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 601 631 805 820 1078 1165 1168 1171
Size: 15 30 174 15 258 87 3 3
Cuts at: 1171 586
Size: 3596
Fragments arranged by size:
3596 258 174 87 30 15 15 3
3
BseYI C'CCAG_C
Cuts at: 198 414 467 759 979 1606 2364 2941 3437
Size: 216 53 292 220 627 758 577 496
Cuts at: 3437 198
Size: 942
Fragments arranged by size:
942 758 627 577 496 292 220 216
53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1029 1029
Size: 4181
BsiEI CG_ry'CG
Cuts at: 104 179 1723 1769 2977 104
Size: 75 1544 46 1208 1308
Fragments arranged by size:
1544 1308 1208 75 46
BsiHKAI G_wGCw'C
Cuts at: 146 1195 1973 2163 2955 146
Size: 1049 778 190 792 1372
Fragments arranged by size:
1372 1049 792 778 190
BsiWI C'GTAC_G
Cuts at: 698 869 698
Size: 171 4010
BsmI GAATG_Cn'
Cuts at: 456 456
Size: 4181
BsmBI CGTCTCn'nnnn_
Cuts at: 233 233
Size: 4181
Bsp1286I G_dGCh'C
Cuts at: 146 287 421 502 583 599 689 752 1101
Size: 141 134 81 81 16 90 63 349
Cuts at: 1101 1195 1806 1899 1973 2163 2225 2955 146
Size: 94 611 93 74 190 62 730 1372
Fragments arranged by size:
1372 730 611 349 190 141 134 94
93 90 81 81 74 63 62 16
BspEI T'CCGG_A
Cuts at: 3401 3401
Size: 4181
BspHI T'CATG_A
Cuts at: 3357 3357
Size: 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1231 1445 1747 2128 1231
Size: 214 302 381 3284
Fragments arranged by size:
3284 381 302 214
BsrBI CCG'CTC
Cuts at: 50 166 396 2473 2527 4011 4176 50
Size: 116 230 2077 54 1484 165 55
Fragments arranged by size:
2077 1484 230 165 116 55 54
BsrDI GCAATG_nn'
Cuts at: 2093 2093
Size: 4181
BsrFI r'CCGG_y
Cuts at: 2179 2360 2569 2179
Size: 181 209 3791
Fragments arranged by size:
3791 209 181
BssSI C'ACGA_G
Cuts at: 2452 2810 2452
Size: 358 3823
BstAPI GCAn_nnn'nTGC
Cuts at: 1558 1558
Size: 4181
BstBI TT'CG_AA
Cuts at: 2542 2542
Size: 4181
BstEII G'GTnAC_C
Cuts at: 381 820 381
Size: 439 3742
BstYI r'GATC_y
Cuts at: 1 663 672 1206 1673 1696 2031 2277 3289
Size: 662 9 534 467 23 335 246 1012
Cuts at: 3289 3375 3387 3733 1
Size: 86 12 346 449
Fragments arranged by size:
1012 662 534 467 449 346 335 246
86 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 1050 1050
Size: 4181
BtgI C'CryG_G
Cuts at: 488 614 825 875 488
Size: 126 211 50 3794
Fragments arranged by size:
3794 211 126 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 581 845 2131 2312 2475 581
Size: 264 1286 181 163 2287
Fragments arranged by size:
2287 1286 264 181 163
BtsI GCAGTG_nn'
Cuts at: 599 1424 599
Size: 825 3356
DraI TTT'AAA
Cuts at: 1180 1180
Size: 4181
DrdI GACnn_nn'nnGTC
Cuts at: 1887 2745 1887
Size: 858 3323
EaeI y'GGCC_r
Cuts at: 176 228 1766 1940 2331 2358 3703 176
Size: 52 1538 174 391 27 1345 654
Fragments arranged by size:
1538 1345 654 391 174 52 27
EagI C'GGCC_G
Cuts at: 176 1766 176
Size: 1590 2591
EarI CTCTTCn'nnn_
Cuts at: 321 2204 2414 321
Size: 1883 210 2088
Fragments arranged by size:
2088 1883 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 199 2389 2699 2845 3991 199
Size: 2190 310 146 1146 389
Fragments arranged by size:
2190 1146 389 310 146
EcoICRI GAG'CTC
Cuts at: 1193 1193
Size: 4181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 970 1023 1081 2006 2438 3185 3453 727
Size: 243 53 58 925 432 747 268 1455
Fragments arranged by size:
1455 925 747 432 268 243 58 53
EcoO109I rG'GnC_Cy
Cuts at: 748 749 798 1097 1320 748
Size: 1 49 299 223 3609
Fragments arranged by size:
3609 299 223 49 1
EcoRI G'AATT_C
Cuts at: 1185 1185
Size: 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 4181
FspI TGC'GCA
Cuts at: 1962 1962
Size: 4181
HaeII r_GCGC'y
Cuts at: 156 194 842 1615 1863 2885 3864 156
Size: 38 648 773 248 1022 979 473
Fragments arranged by size:
1022 979 773 648 473 248 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 913 945 1015 1047 2023 2053 2055
Size: 32 601 32 70 32 976 30 2
Cuts at: 2055 2085 280
Size: 30 2376
Fragments arranged by size:
2376 976 601 70 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1220 1220
Size: 4181
KasI G'GCGC_C
Cuts at: 838 1611 1859 838
Size: 773 248 3160
Fragments arranged by size:
3160 773 248
KpnI G_GTAC'C
Cuts at: 1201 1201
Size: 4181
MluI A'CGCG_T
Cuts at: 3614 3614
Size: 4181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 583 2852 3036 583
Size: 2269 184 1728
Fragments arranged by size:
2269 1728 184
MscI TGG'CCA
Cuts at: 1942 1942
Size: 4181
MslI CAynn'nnrTG
Cuts at: 830 2170 2297 830
Size: 1340 127 2714
Fragments arranged by size:
2714 1340 127
MspA1I CmG'CkG
Cuts at: 198 490 616 844 973 1043 1292 1606 1966
Size: 292 126 228 129 70 249 314 360
Cuts at: 1966 2979 3224 198
Size: 1013 245 1155
Fragments arranged by size:
1155 1013 360 314 292 249 245 228
129 126 70
NaeI GCC'GGC
Cuts at: 2362 2362
Size: 4181
NarI GG'CG_CC
Cuts at: 839 1612 1860 839
Size: 773 248 3160
Fragments arranged by size:
3160 773 248
NcoI C'CATG_G
Cuts at: 825 825
Size: 4181
NgoMIV G'CCGG_C
Cuts at: 2360 2360
Size: 4181
NspI r_CATG'y
Cuts at: 414 715 1234 1734 2641 414
Size: 301 519 500 907 1954
Fragments arranged by size:
1954 907 519 500 301
PciI A'CATG_T
Cuts at: 2637 2637
Size: 4181
PflMI CCAn_nnn'nTGG
Cuts at: 948 948
Size: 4181
PfoI T'CCnGG_A
Cuts at: 346 1058 346
Size: 712 3469
PmeI GTTT'AAAC
Cuts at: 1180 1180
Size: 4181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 4149
PpuMI rG'GwC_Cy
Cuts at: 798 798
Size: 4181
PspOMI G'GGCC_C
Cuts at: 748 1097 748
Size: 349 3832
PstI C_TGCA'G
Cuts at: 1228 1913 1228
Size: 685 3496
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4181
PvuII CAG'CTG
Cuts at: 1043 1606 1966 1043
Size: 563 360 3258
Fragments arranged by size:
3258 563 360
RsrII CG'GwC_CG
Cuts at: 2376 2376
Size: 4181
SacI G_AGCT'C
Cuts at: 1195 1195
Size: 4181
SacII CC_GC'GG
Cuts at: 491 617 491
Size: 126 4055
SalI G'TCGA_C
Cuts at: 1218 1218
Size: 4181
SanDI GG'GwC_CC
Cuts at: 798 798
Size: 4181
SapI GCTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
SbfI CC_TGCA'GG
Cuts at: 1228 1228
Size: 4181
ScaI AGT'ACT
Cuts at: 3698 3698
Size: 4181
SfcI C'TryA_G
Cuts at: 33 1224 1909 2902 3093 33
Size: 1191 685 993 191 1121
Fragments arranged by size:
1191 1121 993 685 191
SfoI GGC'GCC
Cuts at: 840 1613 1861 840
Size: 773 248 3160
Fragments arranged by size:
3160 773 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
SmaI CCC'GGG
Cuts at: 1102 1203 1102
Size: 101 4080
SmlI C'TyrA_G
Cuts at: 118 1113 1336 2251 2743 3005 118
Size: 995 223 915 492 262 1294
Fragments arranged by size:
1294 995 915 492 262 223
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4181
SphI G_CATG'C
Cuts at: 715 1234 1734 715
Size: 519 500 3162
Fragments arranged by size:
3162 519 500
SrfI GCCC'GGGC
Cuts at: 1102 1102
Size: 4181
StuI AGG'CCT
Cuts at: 655 655
Size: 4181
StyI C'CwwG_G
Cuts at: 299 689 825 1315 1669 299
Size: 390 136 490 354 2811
Fragments arranged by size:
2811 490 390 354 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 751 871 1764 1897 2356 2565 751
Size: 120 893 133 459 209 2367
Fragments arranged by size:
2367 893 459 209 133 120
TatI w'GTAC_w
Cuts at: 3696 3696
Size: 4181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 846 878 946 978 3698 3730 846
Size: 32 68 32 2720 32 1297
Fragments arranged by size:
2720 1297 68 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1978 1978
Size: 4181
XbaI T'CTAG_A
Cuts at: 1212 1212
Size: 4181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1676 1676
Size: 4181
XmaI C'CCGG_G
Cuts at: 1100 1201 1100
Size: 101 4080
ZraI GAC'GTC
Cuts at: 768 768
Size: 4181
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsrGI BssHII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SspI SwaI XhoI XmnI