Restriction map for
pF1KB4173
(Circular) (Six-base) MAPSORT of: pF1KB4173.seq Check: 5789 from: 1 to: 5969
pF1KB4173 5969 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2484 2484
Size: 5969
AatII G_ACGT'C
Cuts at: 359 1307 2045 359
Size: 948 738 4283
Fragments arranged by size:
4283 948 738
AccI GT'mk_AC
Cuts at: 1102 1124 3007 1102
Size: 22 1883 4064
Fragments arranged by size:
4064 1883 22
Acc65I G'GTAC_C
Cuts at: 2985 2985
Size: 5969
AclI AA'CG_TT
Cuts at: 5809 5809
Size: 5969
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 2151 3794 4226 4579 5241 829
Size: 1322 1643 432 353 662 1557
Fragments arranged by size:
1643 1557 1322 662 432 353
AflIII A'CryG_T
Cuts at: 1955 2778 3321 5121 5402 1955
Size: 823 543 1800 281 2522
Fragments arranged by size:
2522 1800 823 543 281
AhdI GACnn_n'nnGTC
Cuts at: 1020 1020
Size: 5969
AleI CACnn'nnGTG
Cuts at: 811 2368 2761 811
Size: 1557 393 4019
Fragments arranged by size:
4019 1557 393
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 517 549 1336 1368 1657 1689 517
Size: 32 787 32 289 32 4797
Fragments arranged by size:
4797 787 289 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 456 1170 1398 1533 2340 3274 4712 456
Size: 714 228 135 807 934 1438 1713
Fragments arranged by size:
1713 1438 934 807 714 228 135
ApaI G_GGCC'C
Cuts at: 302 1141 1290 1459 2593 302
Size: 839 149 169 1134 3678
Fragments arranged by size:
3678 1134 839 169 149
ApaLI G'TGCA_C
Cuts at: 772 4807 772
Size: 4035 1934
ApoI r'AATT_y
Cuts at: 2973 5322 5578 2973
Size: 2349 256 3364
Fragments arranged by size:
3364 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5969
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5969
AvaI C'yCGr_G
Cuts at: 224 302 1452 1552 1877 2666 2989 224
Size: 78 1150 100 325 789 323 3204
Fragments arranged by size:
3204 1150 789 325 323 100 78
BamHI G'GATC_C
Cuts at: 1112 1434 2994 1112
Size: 322 1560 4087
Fragments arranged by size:
4087 1560 322
BanI G'GyrC_C
Cuts at: 119 684 717 816 1635 2022 2439 2841 2985
Size: 565 33 99 819 387 417 402 144
Cuts at: 2985 3399 3647 3682 119
Size: 414 248 35 2406
Fragments arranged by size:
2406 819 565 417 414 402 387 248
144 99 35 33
BanII G_rGCy'C
Cuts at: 302 468 535 758 1084 1141 1148 1290 1459
Size: 166 67 223 326 57 7 142 169
Cuts at: 1459 1693 1966 2593 2983 4013 302
Size: 234 273 627 390 1030 2258
Fragments arranged by size:
2258 1030 627 390 326 273 234 223
169 166 142 67 57 7
BbeI G_GCGC'C
Cuts at: 820 2845 3403 3651 820
Size: 2025 558 248 3138
Fragments arranged by size:
3138 2025 558 248
BciVI GTATCCnnnnn_n'
Cuts at: 1177 3867 4923 5969 1177
Size: 2690 1056 1046 1177
Fragments arranged by size:
2690 1177 1056 1046
BclI T'GATC_A
Cuts at: 3489 3489
Size: 5969
BglI GCCn_nnn'nGGC
Cuts at: 1235 2185 2386 1235
Size: 950 201 4818
Fragments arranged by size:
4818 950 201
BglII A'GATC_T
Cuts at: 3484 5521 3484
Size: 2037 3932
BlpI GC'TnA_GC
Cuts at: 913 3081 913
Size: 2168 3801
Bme1580I G_kGCm'C
Cuts at: 194 302 344 689 720 776 1141 1290 1459
Size: 108 42 345 31 56 365 149 169
Cuts at: 1459 1640 2419 2593 3594 3687 4811 194
Size: 181 779 174 1001 93 1124 1352
Fragments arranged by size:
1352 1124 1001 779 365 345 181 174
169 149 108 93 56 42 31
BmgBI CAC'GTC
Cuts at: 618 1956 2259 618
Size: 1338 303 4328
Fragments arranged by size:
4328 1338 303
BmrI ACTGGGnnnn_n'
Cuts at: 241 1215 2362 3181 3262 3597 5741 241
Size: 974 1147 819 81 335 2144 469
Fragments arranged by size:
2144 1147 974 819 469 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4336 4336
Size: 5969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 541 1030 1116 1741 1747 1770 2314 2359
Size: 267 489 86 625 6 23 544 45
Cuts at: 2359 2731 274
Size: 372 3512
Fragments arranged by size:
3512 625 544 489 372 267 86 45
23 6
Bpu10I CC'TnA_GC
Cuts at: 529 1090 529
Size: 561 5408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3145 4024 4738 5036 3145
Size: 879 714 298 4078
Fragments arranged by size:
4078 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 688 992 1513 2263 5673 688
Size: 304 521 750 3410 984
Fragments arranged by size:
3410 984 750 521 304
BsaAI yAC'GTr
Cuts at: 1737 2779 3322 1737
Size: 1042 543 4384
Fragments arranged by size:
4384 1042 543
BsaBI GATnn'nnATC
Cuts at: 6 3483 5520 6
Size: 3477 2037 455
Fragments arranged by size:
3477 2037 455
BsaHI Gr'CG_yC
Cuts at: 356 817 1304 2042 2842 3400 3648 4350 5846
Size: 461 487 738 800 558 248 702 1496
Cuts at: 5846 356
Size: 479
Fragments arranged by size:
1496 800 738 702 558 487 479 461
248
BsaWI w'CCGG_w
Cuts at: 3158 3679 4357 4768 4915 5189 3158
Size: 521 678 411 147 274 3938
Fragments arranged by size:
3938 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 517 547 1318 1348 517
Size: 30 771 30 5138
Fragments arranged by size:
5138 771 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 231 271 525 766 1233 1974 2086 2212 231
Size: 40 254 241 467 741 112 126 3988
Fragments arranged by size:
3988 741 467 254 241 126 112 40
BseYI C'CCAG_C
Cuts at: 267 499 508 1232 2016 2093 2097 2180 2566
Size: 232 9 724 784 77 4 83 386
Cuts at: 2566 2846 3394 4152 4817 5225 267
Size: 280 548 758 665 408 1011
Fragments arranged by size:
1011 784 758 724 665 548 408 386
280 232 83 77 9 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 1180 1384 1411 1804 164
Size: 1016 204 27 393 4329
Fragments arranged by size:
4329 1016 393 204 27
BsiEI CG_ry'CG
Cuts at: 104 327 792 1413 1929 2762 3511 3557 4787
Size: 223 465 621 516 833 749 46 1230
Cuts at: 4787 104
Size: 1286
Fragments arranged by size:
1286 1230 833 749 621 516 465 223
46
BsiHKAI G_wGCw'C
Cuts at: 535 776 896 1966 2375 2983 3761 3951 4811
Size: 241 120 1070 409 608 778 190 860
Cuts at: 4811 535
Size: 1693
Fragments arranged by size:
1693 1070 860 778 608 409 241 190
120
Bsp1286I G_dGCh'C
Cuts at: 194 302 344 468 535 689 720 758 776
Size: 108 42 124 67 154 31 38 18
Cuts at: 776 896 1084 1141 1148 1290 1459 1640 1693
Size: 120 188 57 7 142 169 181 53
Cuts at: 1693 1966 2375 2419 2593 2983 3594 3687 3761
Size: 273 409 44 174 390 611 93 74
Cuts at: 3761 3951 4013 4811 194
Size: 190 62 798 1352
Fragments arranged by size:
1352 798 611 409 390 273 190 188
181 174 169 154 142 124 120 108
93 74 67 62 57 53 44 42
38 31 18 7
BspHI T'CATG_A
Cuts at: 4401 4401
Size: 5969
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1826 2021 2438 2484 2841 3019 3233 3535 3916
Size: 195 417 46 357 178 214 302 381
Cuts at: 3916 1826
Size: 3879
Fragments arranged by size:
3879 417 381 357 302 214 195 178
46
BsrBI CCG'CTC
Cuts at: 50 1907 4261 4315 5799 5964 50
Size: 1857 2354 54 1484 165 55
Fragments arranged by size:
2354 1857 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1059 3881 1059
Size: 2822 3147
BsrFI r'CCGG_y
Cuts at: 137 776 1412 1522 1936 2175 2529 3967 4148
Size: 639 636 110 414 239 354 1438 181
Cuts at: 4148 137
Size: 1958
Fragments arranged by size:
1958 1438 639 636 414 354 239 181
110
BssSI C'ACGA_G
Cuts at: 160 4240 4948 160
Size: 4080 708 1181
Fragments arranged by size:
4080 1181 708
BstAPI GCAn_nnn'nTGC
Cuts at: 178 456 1398 2439 3346 178
Size: 278 942 1041 907 2801
Fragments arranged by size:
2801 1041 942 907 278
BstBI TT'CG_AA
Cuts at: 4330 4330
Size: 5969
BstXI CCAn_nnnn'nTGG
Cuts at: 1065 1155 1593 2634 2910 1065
Size: 90 438 1041 276 4124
Fragments arranged by size:
4124 1041 438 276 90
BstYI r'GATC_y
Cuts at: 1 660 1112 1434 2994 3461 3484 3819 4065
Size: 659 452 322 1560 467 23 335 246
Cuts at: 4065 4371 4383 4469 5521 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1560 1052 659 467 452 449 335 322
306 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 423 1058 1385 1923 2273 2627 2837 107
Size: 316 635 327 538 350 354 210 3239
Fragments arranged by size:
3239 635 538 354 350 327 316 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1833 3919 4100 4263 1833
Size: 2086 181 163 3539
Fragments arranged by size:
3539 2086 181 163
BtsI GCAGTG_nn'
Cuts at: 344 1559 2521 3212 344
Size: 1215 962 691 3101
Fragments arranged by size:
3101 1215 962 691
DraI TTT'AAA
Cuts at: 2968 2968
Size: 5969
DraIII CAC_nnn'GTG
Cuts at: 350 941 1035 350
Size: 591 94 5284
Fragments arranged by size:
5284 591 94
DrdI GACnn_nn'nnGTC
Cuts at: 2905 2920 3675 5019 2905
Size: 15 755 1344 3855
Fragments arranged by size:
3855 1344 755 15
EaeI y'GGCC_r
Cuts at: 146 324 554 789 1382 1410 1524 1769 1920
Size: 178 230 235 593 28 114 245 151
Cuts at: 1920 1926 1938 2744 2834 3554 3728 4119 4146
Size: 6 12 806 90 720 174 391 27
Cuts at: 4146 5491 146
Size: 1345 624
Fragments arranged by size:
1345 806 720 624 593 391 245 235
230 178 174 151 114 90 28 27
12 6
EagI C'GGCC_G
Cuts at: 324 789 1410 1926 3554 324
Size: 465 621 516 1628 2739
Fragments arranged by size:
2739 1628 621 516 465
EarI CTCTTCn'nnn_
Cuts at: 2293 3992 4202 2293
Size: 1699 210 4060
Fragments arranged by size:
4060 1699 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4177 4919 5065 5779 4177
Size: 742 146 714 4367
Fragments arranged by size:
4367 742 714 146
EcoICRI GAG'CTC
Cuts at: 533 1964 2981 533
Size: 1431 1017 3521
Fragments arranged by size:
3521 1431 1017
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 541 829 1030 1116 1741 1747 1770 2151
Size: 267 288 201 86 625 6 23 381
Cuts at: 2151 2314 2359 2731 3794 4226 4579 5241 274
Size: 163 45 372 1063 432 353 662 1002
Fragments arranged by size:
1063 1002 662 625 432 381 372 353
288 267 201 163 86 45 23 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1857 2650 1857
Size: 793 5176
EcoO109I rG'GnC_Cy
Cuts at: 298 299 951 1209 1286 1456 1475 1757 2102
Size: 1 652 258 77 170 19 282 345
Cuts at: 2102 2199 2352 2451 2589 2590 2720 2853 2907
Size: 97 153 99 138 1 130 133 54
Cuts at: 2907 3108 298
Size: 201 3159
Fragments arranged by size:
3159 652 345 282 258 201 170 153
138 133 130 99 97 77 54 19
1 1
EcoRI G'AATT_C
Cuts at: 2973 2973
Size: 5969
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1383 3988 1383
Size: 2605 3364
FspI TGC'GCA
Cuts at: 3750 3750
Size: 5969
HaeII r_GCGC'y
Cuts at: 678 820 1009 1419 1867 2845 3403 3651 4881
Size: 142 189 410 448 978 558 248 1230
Cuts at: 4881 5652 678
Size: 771 995
Fragments arranged by size:
1230 995 978 771 558 448 410 248
189 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 645 677 1006 1038 1419 1451 3811 3841 3843
Size: 32 329 32 381 32 2360 30 2
Cuts at: 3843 3873 645
Size: 30 2741
Fragments arranged by size:
2741 2360 381 329 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1103 3008 1103
Size: 1905 4064
KasI G'GCGC_C
Cuts at: 816 2841 3399 3647 816
Size: 2025 558 248 3138
Fragments arranged by size:
3138 2025 558 248
KpnI G_GTAC'C
Cuts at: 2989 2989
Size: 5969
MluI A'CGCG_T
Cuts at: 5402 5402
Size: 5969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1700 2885 2903 4728 4912 1700
Size: 1185 18 1825 184 2757
Fragments arranged by size:
2757 1825 1185 184 18
MscI TGG'CCA
Cuts at: 1922 2746 2836 3730 1922
Size: 824 90 894 4161
Fragments arranged by size:
4161 894 824 90
MslI CAynn'nnrTG
Cuts at: 112 811 1063 2368 2761 3958 4085 112
Size: 699 252 1305 393 1197 127 1996
Fragments arranged by size:
1996 1305 1197 699 393 252 127
MspA1I CmG'CkG
Cuts at: 178 425 783 849 923 1232 1533 2097 2808
Size: 247 358 66 74 309 301 564 711
Cuts at: 2808 2957 3080 3394 3754 4538 4783 178
Size: 149 123 314 360 784 245 1364
Fragments arranged by size:
1364 784 711 564 360 358 314 309
301 247 245 149 123 74 66
NaeI GCC'GGC
Cuts at: 1414 1938 2177 2531 4150 1414
Size: 524 239 354 1619 3233
Fragments arranged by size:
3233 1619 524 354 239
NarI GG'CG_CC
Cuts at: 817 2842 3400 3648 817
Size: 2025 558 248 3138
Fragments arranged by size:
3138 2025 558 248
NcoI C'CATG_G
Cuts at: 107 1058 1385 2627 107
Size: 951 327 1242 3449
Fragments arranged by size:
3449 1242 951 327
NgoMIV G'CCGG_C
Cuts at: 1412 1936 2175 2529 4148 1412
Size: 524 239 354 1619 3233
Fragments arranged by size:
3233 1619 524 354 239
NspI r_CATG'y
Cuts at: 1653 3022 3522 5125 1653
Size: 1369 500 1603 2497
Fragments arranged by size:
2497 1603 1369 500
PciI A'CATG_T
Cuts at: 5121 5121
Size: 5969
PflMI CCAn_nnn'nTGG
Cuts at: 1269 1269
Size: 5969
PfoI T'CCnGG_A
Cuts at: 251 1115 1336 1370 2291 2909 251
Size: 864 221 34 921 618 3311
Fragments arranged by size:
3311 921 864 618 221 34
PmeI GTTT'AAAC
Cuts at: 2968 2968
Size: 5969
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 517 549 4415 4447 517
Size: 32 3866 32 2039
Fragments arranged by size:
3866 2039 32 32
PpuMI rG'GwC_Cy
Cuts at: 951 1757 2853 2907 951
Size: 806 1096 54 4013
Fragments arranged by size:
4013 1096 806 54
PshAI GACnn'nnGTC
Cuts at: 1487 1487
Size: 5969
PspOMI G'GGCC_C
Cuts at: 298 1137 1286 1455 2589 298
Size: 839 149 169 1134 3678
Fragments arranged by size:
3678 1134 839 169 149
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1717 1749 1717
Size: 32 5937
PstI C_TGCA'G
Cuts at: 461 1076 2006 2435 2481 2588 2807 2813 3016
Size: 615 930 429 46 107 219 6 203
Cuts at: 3016 3701 461
Size: 685 2729
Fragments arranged by size:
2729 930 685 615 429 219 203 107
46 6
PvuI CG_AT'CG
Cuts at: 104 2762 104
Size: 2658 3311
PvuII CAG'CTG
Cuts at: 783 849 923 2097 2808 2957 3394 3754 783
Size: 66 74 1174 711 149 437 360 2998
Fragments arranged by size:
2998 1174 711 437 360 149 74 66
RsrII CG'GwC_CG
Cuts at: 4164 4164
Size: 5969
SacI G_AGCT'C
Cuts at: 535 1966 2983 535
Size: 1431 1017 3521
Fragments arranged by size:
3521 1431 1017
SacII CC_GC'GG
Cuts at: 426 426
Size: 5969
SalI G'TCGA_C
Cuts at: 1101 3006 1101
Size: 1905 4064
SanDI GG'GwC_CC
Cuts at: 1757 1757
Size: 5969
SapI GCTCTTCn'nnn_
Cuts at: 3992 4202 3992
Size: 210 5759
SbfI CC_TGCA'GG
Cuts at: 2588 3016 2588
Size: 428 5541
ScaI AGT'ACT
Cuts at: 5486 5486
Size: 5969
SexAI A'CCwGG_T
Cuts at: 1631 1631
Size: 5969
SfcI C'TryA_G
Cuts at: 33 457 732 1072 1470 2002 2431 2477 2584
Size: 424 275 340 398 532 429 46 107
Cuts at: 2584 2803 2809 2889 3012 3697 4665 4856 33
Size: 219 6 80 123 685 968 191 1146
Fragments arranged by size:
1146 968 685 532 429 424 398 340
275 219 191 123 107 80 46 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2185 2386 2185
Size: 201 5768
SfoI GGC'GCC
Cuts at: 818 2843 3401 3649 818
Size: 2025 558 248 3138
Fragments arranged by size:
3138 2025 558 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5969
SmaI CCC'GGG
Cuts at: 304 1554 2991 304
Size: 1250 1437 3282
Fragments arranged by size:
3282 1437 1250
SmlI C'TyrA_G
Cuts at: 3124 4039 4753 5015 3124
Size: 915 714 262 4078
Fragments arranged by size:
4078 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5969
SphI G_CATG'C
Cuts at: 3022 3522 3022
Size: 500 5469
StuI AGG'CCT
Cuts at: 884 884
Size: 5969
StyI C'CwwG_G
Cuts at: 107 167 590 689 1058 1385 2303 2411 2454
Size: 60 423 99 369 327 918 108 43
Cuts at: 2454 2627 2651 2903 3103 3457 107
Size: 173 24 252 200 354 2619
Fragments arranged by size:
2619 918 423 369 354 327 252 200
173 108 99 60 43 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 281 418 513 1855 2508 3552 3685 4144 4353
Size: 137 95 1342 653 1044 133 459 209
Cuts at: 4353 281
Size: 1897
Fragments arranged by size:
1897 1342 1044 653 459 209 137 133
95
TatI w'GTAC_w
Cuts at: 5484 5484
Size: 5969
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5486 5518 5486
Size: 32 5937
Tth111I GACn'n_nGTC
Cuts at: 3766 3766
Size: 5969
XbaI T'CTAG_A
Cuts at: 3000 3000
Size: 5969
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 516 1534 1716 2563 3464 516
Size: 1018 182 847 901 3021
Fragments arranged by size:
3021 1018 901 847 182
XmaI C'CCGG_G
Cuts at: 302 1552 2989 302
Size: 1250 1437 3282
Fragments arranged by size:
3282 1437 1250
ZraI GAC'GTC
Cuts at: 357 1305 2043 357
Size: 948 738 4283
Fragments arranged by size:
4283 948 738
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AlfI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BmtI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PsiI PspXI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI