Restriction map for
pF1KB4171
(Circular) (Six-base) MAPSORT of: pF1KB4171.seq Check: 8334 from: 1 to: 6566
pF1KB4171 6566 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1662 3604 1662
Size: 1942 4624
Acc65I G'GTAC_C
Cuts at: 2316 3582 2316
Size: 1266 5300
AclI AA'CG_TT
Cuts at: 6406 6406
Size: 6566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2178 2722 3034 4391 4823 5570 5838 2178
Size: 544 312 1357 432 747 268 2906
Fragments arranged by size:
2906 1357 747 544 432 312 268
AfeI AGC'GCT
Cuts at: 1009 1009
Size: 6566
AflIII A'CryG_T
Cuts at: 306 746 1937 3918 5022 5999 306
Size: 440 1191 1981 1104 977 873
Fragments arranged by size:
1981 1191 1104 977 873 440
AgeI A'CCGG_T
Cuts at: 4954 4954
Size: 6566
AhdI GACnn_n'nnGTC
Cuts at: 1209 1209
Size: 6566
AleI CACnn'nnGTG
Cuts at: 3544 3544
Size: 6566
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2843 3478 3517 2843
Size: 635 39 5892
Fragments arranged by size:
5892 635 39
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 613 645 2050 2082 613
Size: 32 1405 32 5097
Fragments arranged by size:
5097 1405 32 32
AlwNI CAG_nnn'CTG
Cuts at: 610 2979 3127 3172 3871 5438 610
Size: 2369 148 45 699 1567 1738
Fragments arranged by size:
2369 1738 1567 699 148 45
ApaI G_GGCC'C
Cuts at: 3204 3223 3204
Size: 19 6547
ApaLI G'TGCA_C
Cuts at: 5336 5336
Size: 6566
ApoI r'AATT_y
Cuts at: 403 3570 5919 6175 403
Size: 3167 2349 256 794
Fragments arranged by size:
3167 2349 794 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6566
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6566
AvaI C'yCGr_G
Cuts at: 651 1447 2255 2474 2522 3522 3586 651
Size: 796 808 219 48 1000 64 3631
Fragments arranged by size:
3631 1000 808 796 219 64 48
AvrII C'CTAG_G
Cuts at: 808 2163 808
Size: 1355 5211
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2944 2977 2944
Size: 33 6533
BamHI G'GATC_C
Cuts at: 752 830 3591 752
Size: 78 2761 3727
Fragments arranged by size:
3727 2761 78
BanI G'GyrC_C
Cuts at: 1231 1731 1818 2316 3404 3493 3582 3996 4244
Size: 500 87 498 1088 89 89 414 248
Cuts at: 4244 4279 1231
Size: 35 3518
Fragments arranged by size:
3518 1088 500 498 414 248 89 89
87 35
BanII G_rGCy'C
Cuts at: 557 1145 1443 2513 3036 3204 3223 3288 3529
Size: 588 298 1070 523 168 19 65 241
Cuts at: 3529 3580 4610 557
Size: 51 1030 2513
Fragments arranged by size:
2513 1070 1030 588 523 298 241 168
65 51 19
BbeI G_GCGC'C
Cuts at: 4000 4248 4000
Size: 248 6318
BbsI GAAGACnn'nnnn_
Cuts at: 878 1124 2113 2138 2740 878
Size: 246 989 25 602 4704
Fragments arranged by size:
4704 989 602 246 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2098 2132 2098
Size: 34 6532
BciVI GTATCCnnnnn_n'
Cuts at: 653 4464 5225 6566 653
Size: 3811 761 1341 653
Fragments arranged by size:
3811 1341 761 653
BclI T'GATC_A
Cuts at: 4086 4086
Size: 6566
BglI GCCn_nnn'nGGC
Cuts at: 2236 2794 3439 2236
Size: 558 645 5363
Fragments arranged by size:
5363 645 558
BglII A'GATC_T
Cuts at: 1487 4081 6118 1487
Size: 2594 2037 1935
Fragments arranged by size:
2594 2037 1935
BlpI GC'TnA_GC
Cuts at: 2816 3678 2816
Size: 862 5704
Bme1580I G_kGCm'C
Cuts at: 544 3204 3223 3407 4191 4284 5340 544
Size: 2660 19 184 784 93 1056 1770
Fragments arranged by size:
2660 1770 1056 784 184 93 19
BmrI ACTGGGnnnn_n'
Cuts at: 168 729 1213 3461 3778 3859 4194 6338 168
Size: 561 484 2248 317 81 335 2144 396
Fragments arranged by size:
2248 2144 561 484 396 335 317 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1869 2313 4933 1869
Size: 444 2620 3502
Fragments arranged by size:
3502 2620 444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1466 2047 2164 2944 2977 3149 3191 3302 3491
Size: 581 117 780 33 172 42 111 189
Cuts at: 3491 1466
Size: 4541
Fragments arranged by size:
4541 780 581 189 172 117 111 42
33
Bpu10I CC'TnA_GC
Cuts at: 1832 2441 1832
Size: 609 5957
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3742 4621 5113 5411 3742
Size: 879 492 298 4897
Fragments arranged by size:
4897 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2636 2869 6270 2636
Size: 233 3401 2932
Fragments arranged by size:
3401 2932 233
BsaAI yAC'GTr
Cuts at: 749 3919 749
Size: 3170 3396
BsaBI GATnn'nnATC
Cuts at: 6 965 4080 6117 6
Size: 959 3115 2037 455
Fragments arranged by size:
3115 2037 959 455
BsaHI Gr'CG_yC
Cuts at: 3997 4245 4947 6443 3997
Size: 248 702 1496 4120
Fragments arranged by size:
4120 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3755 4276 4954 5228 5375 5786 3755
Size: 521 678 274 147 411 4535
Fragments arranged by size:
4535 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 613 643 889 919 613
Size: 30 246 30 6260
Fragments arranged by size:
6260 246 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1123 2305 2467 2560 2605 2932 3356 1123
Size: 1182 162 93 45 327 424 4333
Fragments arranged by size:
4333 1182 424 327 162 93 45
BseYI C'CCAG_C
Cuts at: 756 1387 1786 3104 3130 3290 3991 4749 5326
Size: 631 399 1318 26 160 701 758 577
Cuts at: 5326 5822 756
Size: 496 1500
Fragments arranged by size:
1500 1318 758 701 631 577 496 399
160 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 700 523
Size: 177 6389
BsiEI CG_ry'CG
Cuts at: 104 4108 4154 5362 104
Size: 4004 46 1208 1308
Fragments arranged by size:
4004 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2224 3580 4358 4548 5340 2224
Size: 1356 778 190 792 3450
Fragments arranged by size:
3450 1356 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1267 1819 1267
Size: 552 6014
Bsp1286I G_dGCh'C
Cuts at: 544 557 1145 1443 2224 2513 3036 3204 3223
Size: 13 588 298 781 289 523 168 19
Cuts at: 3223 3288 3407 3529 3580 4191 4284 4358 4548
Size: 65 119 122 51 611 93 74 190
Cuts at: 4548 4610 5340 544
Size: 62 730 1770
Fragments arranged by size:
1770 781 730 611 588 523 298 289
190 168 122 119 93 74 65 62
51 19 13
BspEI T'CCGG_A
Cuts at: 5786 5786
Size: 6566
BspHI T'CATG_A
Cuts at: 689 1598 5742 689
Size: 909 4144 1513
Fragments arranged by size:
4144 1513 909
BspMI ACCTGCnnnn'nnnn_
Cuts at: 696 1349 3616 3830 4132 4513 696
Size: 653 2267 214 302 381 2749
Fragments arranged by size:
2749 2267 653 381 302 214
BsrBI CCG'CTC
Cuts at: 50 324 1434 2445 2459 2748 4858 4912 6396
Size: 274 1110 1011 14 289 2110 54 1484
Cuts at: 6396 6561 50
Size: 165 55
Fragments arranged by size:
2110 1484 1110 1011 289 274 165 55
54 14
BsrDI GCAATG_nn'
Cuts at: 321 471 2414 4478 321
Size: 150 1943 2064 2409
Fragments arranged by size:
2409 2064 1943 150
BsrFI r'CCGG_y
Cuts at: 4564 4745 4954 4564
Size: 181 209 6176
Fragments arranged by size:
6176 209 181
BsrGI T'GTAC_A
Cuts at: 267 1940 2042 267
Size: 1673 102 4791
Fragments arranged by size:
4791 1673 102
BssSI C'ACGA_G
Cuts at: 1991 4837 5195 1991
Size: 2846 358 3362
Fragments arranged by size:
3362 2846 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1984 3943 1984
Size: 1959 4607
BstBI TT'CG_AA
Cuts at: 2346 4927 2346
Size: 2581 3985
BstEII G'GTnAC_C
Cuts at: 3537 3537
Size: 6566
BstXI CCAn_nnnn'nTGG
Cuts at: 2328 2328
Size: 6566
BstYI r'GATC_y
Cuts at: 1 752 830 1487 3591 4058 4081 4416 4662
Size: 751 78 657 2104 467 23 335 246
Cuts at: 4662 5674 5760 5772 6118 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2104 1012 751 657 467 449 346 335
246 86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 1754 1754
Size: 6566
BtgI C'CryG_G
Cuts at: 107 158 2321 3147 3333 107
Size: 51 2163 826 186 3340
Fragments arranged by size:
3340 2163 826 186 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4516 4697 4860 4516
Size: 181 163 6222
Fragments arranged by size:
6222 181 163
BtsI GCAGTG_nn'
Cuts at: 2415 2813 3201 3395 3478 3809 2415
Size: 398 388 194 83 331 5172
Fragments arranged by size:
5172 398 388 331 194 83
DraI TTT'AAA
Cuts at: 3565 3565
Size: 6566
DrdI GACnn_nn'nnGTC
Cuts at: 2650 4272 5130 2650
Size: 1622 858 4086
Fragments arranged by size:
4086 1622 858
EaeI y'GGCC_r
Cuts at: 1095 2795 2981 4151 4325 4716 4743 6088 1095
Size: 1700 186 1170 174 391 27 1345 1573
Fragments arranged by size:
1700 1573 1345 1170 391 186 174 27
EagI C'GGCC_G
Cuts at: 4151 4151
Size: 6566
EarI CTCTTCn'nnn_
Cuts at: 662 688 994 1100 1160 2306 2626 4589 4799
Size: 26 306 106 60 1146 320 1963 210
Cuts at: 4799 662
Size: 2429
Fragments arranged by size:
2429 1963 1146 320 306 210 106 60
26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1673 2398 4774 5084 5230 6376 1673
Size: 725 2376 310 146 1146 1863
Fragments arranged by size:
2376 1863 1146 725 310 146
EcoICRI GAG'CTC
Cuts at: 3578 3578
Size: 6566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1466 2047 2164 2178 2722 2944 2977 3034 3149
Size: 581 117 14 544 222 33 57 115
Cuts at: 3149 3191 3302 3491 4391 4823 5570 5838 1466
Size: 42 111 189 900 432 747 268 2194
Fragments arranged by size:
2194 900 747 581 544 432 268 222
189 117 115 111 57 42 33 14
EcoNI CCTnn'n_nnAGG
Cuts at: 1126 1345 1886 1126
Size: 219 541 5806
Fragments arranged by size:
5806 541 219
EcoO109I rG'GnC_Cy
Cuts at: 1063 1678 2167 3200 3201 3220 3705 1063
Size: 615 489 1033 1 19 485 3924
Fragments arranged by size:
3924 1033 615 489 485 19 1
EcoRI G'AATT_C
Cuts at: 3570 3570
Size: 6566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1564 4585 1564
Size: 3021 3545
FspI TGC'GCA
Cuts at: 1930 4347 1930
Size: 2417 4149
HaeII r_GCGC'y
Cuts at: 1011 4000 4248 5270 6249 1011
Size: 2989 248 1022 979 1328
Fragments arranged by size:
2989 1328 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1129 1161 1195 1227 1317 1349 1705 1737 2608
Size: 32 34 32 90 32 356 32 871
Cuts at: 2608 2640 3018 3050 4408 4438 4440 4470 1129
Size: 32 378 32 1358 30 2 30 3225
Fragments arranged by size:
3225 1358 871 378 356 90 34 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1191 1663 3251 3605 1191
Size: 472 1588 354 4152
Fragments arranged by size:
4152 1588 472 354
KasI G'GCGC_C
Cuts at: 3996 4244 3996
Size: 248 6318
KpnI G_GTAC'C
Cuts at: 2320 3586 2320
Size: 1266 5300
MluI A'CGCG_T
Cuts at: 5999 5999
Size: 6566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 817 2873 3075 5237 5421 817
Size: 2056 202 2162 184 1962
Fragments arranged by size:
2162 2056 1962 202 184
MscI TGG'CCA
Cuts at: 1097 4327 1097
Size: 3230 3336
MslI CAynn'nnrTG
Cuts at: 112 1700 1936 3544 4555 4682 112
Size: 1588 236 1608 1011 127 1996
Fragments arranged by size:
1996 1608 1588 1011 236 127
MspA1I CmG'CkG
Cuts at: 160 394 2779 3012 3280 3401 3445 3677 3991
Size: 234 2385 233 268 121 44 232 314
Cuts at: 3991 4351 5364 5609 160
Size: 360 1013 245 1117
Fragments arranged by size:
2385 1117 1013 360 314 268 245 234
233 232 121 44
NaeI GCC'GGC
Cuts at: 4747 4747
Size: 6566
NarI GG'CG_CC
Cuts at: 3997 4245 3997
Size: 248 6318
NcoI C'CATG_G
Cuts at: 107 3147 3333 107
Size: 3040 186 3340
Fragments arranged by size:
3340 3040 186
NgoMIV G'CCGG_C
Cuts at: 4745 4745
Size: 6566
NspI r_CATG'y
Cuts at: 310 1914 1941 2077 3619 4119 5026 310
Size: 1604 27 136 1542 500 907 1850
Fragments arranged by size:
1850 1604 1542 907 500 136 27
PciI A'CATG_T
Cuts at: 306 1937 5022 306
Size: 1631 3085 1850
Fragments arranged by size:
3085 1850 1631
PflMI CCAn_nnn'nTGG
Cuts at: 1707 3153 1707
Size: 1446 5120
PfoI T'CCnGG_A
Cuts at: 833 2024 3207 833
Size: 1191 1183 4192
Fragments arranged by size:
4192 1191 1183
PmeI GTTT'AAAC
Cuts at: 3565 3565
Size: 6566
PmlI CAC'GTG
Cuts at: 749 749
Size: 6566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 613 645 5705 5737 613
Size: 32 5060 32 1442
Fragments arranged by size:
5060 1442 32 32
PpuMI rG'GwC_Cy
Cuts at: 1063 1063
Size: 6566
PspOMI G'GGCC_C
Cuts at: 3200 3219 3200
Size: 19 6547
PstI C_TGCA'G
Cuts at: 1742 1980 3258 3613 4298 1742
Size: 238 1278 355 685 4010
Fragments arranged by size:
4010 1278 685 355 238
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6566
PvuII CAG'CTG
Cuts at: 394 3012 3280 3445 3991 4351 394
Size: 2618 268 165 546 360 2609
Fragments arranged by size:
2618 2609 546 360 268 165
RsrII CG'GwC_CG
Cuts at: 4761 4761
Size: 6566
SacI G_AGCT'C
Cuts at: 3580 3580
Size: 6566
SacII CC_GC'GG
Cuts at: 161 161
Size: 6566
SalI G'TCGA_C
Cuts at: 1661 3603 1661
Size: 1942 4624
SapI GCTCTTCn'nnn_
Cuts at: 4589 4799 4589
Size: 210 6356
SbfI CC_TGCA'GG
Cuts at: 3613 3613
Size: 6566
ScaI AGT'ACT
Cuts at: 148 6083 148
Size: 5935 631
SexAI A'CCwGG_T
Cuts at: 1643 1814 1643
Size: 171 6395
SfcI C'TryA_G
Cuts at: 33 1738 1976 2540 3173 3254 3609 4294 5287
Size: 1705 238 564 633 81 355 685 993
Cuts at: 5287 5478 33
Size: 191 1121
Fragments arranged by size:
1705 1121 993 685 633 564 355 238
191 81
SfoI GGC'GCC
Cuts at: 3998 4246 3998
Size: 248 6318
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6566
SmaI CCC'GGG
Cuts at: 653 3588 653
Size: 2935 3631
SmlI C'TyrA_G
Cuts at: 3721 4636 5128 5390 3721
Size: 915 492 262 4897
Fragments arranged by size:
4897 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6566
SphI G_CATG'C
Cuts at: 3619 4119 3619
Size: 500 6066
StuI AGG'CCT
Cuts at: 2158 2158
Size: 6566
StyI C'CwwG_G
Cuts at: 107 497 808 1781 2163 2327 2513 3147 3333
Size: 390 311 973 382 164 186 634 186
Cuts at: 3333 3700 4054 107
Size: 367 354 2619
Fragments arranged by size:
2619 973 634 390 382 367 354 311
186 186 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 252 1255 2386 3422 4149 4282 4741 4950 252
Size: 1003 1131 1036 727 133 459 209 1868
Fragments arranged by size:
1868 1131 1036 1003 727 459 209 133
TatI w'GTAC_w
Cuts at: 146 267 1940 2042 6081 146
Size: 121 1673 102 4039 631
Fragments arranged by size:
4039 1673 631 121 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3128 3160 6083 6115 3128
Size: 32 2923 32 3579
Fragments arranged by size:
3579 2923 32 32
Tth111I GACn'n_nGTC
Cuts at: 4363 4363
Size: 6566
XbaI T'CTAG_A
Cuts at: 660 3597 660
Size: 2937 3629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1242 2328 4061 1242
Size: 1086 1733 3747
Fragments arranged by size:
3747 1733 1086
XmaI C'CCGG_G
Cuts at: 651 3586 651
Size: 2935 3631
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AscI BbvCI BfrBI BmgBI BmtI
BsiWI BsmI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PshAI PsiI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI