Restriction map for
pF1KB4163
(Circular) (Six-base) MAPSORT of: pF1KB4163.seq Check: 7414 from: 1 to: 5930
pF1KB4163 5930 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 314 1826 2968 314
Size: 1512 1142 3276
Fragments arranged by size:
3276 1512 1142
Acc65I G'GTAC_C
Cuts at: 2946 2946
Size: 5930
AclI AA'CG_TT
Cuts at: 2924 5770 2924
Size: 2846 3084
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 322 517 1078 1775 2544 2806 2881 3755
Size: 141 195 561 697 769 262 75 874
Cuts at: 3755 4187 4934 5202 181
Size: 432 747 268 909
Fragments arranged by size:
909 874 769 747 697 561 432 268
262 195 141 75
AfeI AGC'GCT
Cuts at: 656 1276 656
Size: 620 5310
AflIII A'CryG_T
Cuts at: 2891 3282 4386 5363 2891
Size: 391 1104 977 3458
Fragments arranged by size:
3458 1104 977 391
AgeI A'CCGG_T
Cuts at: 4318 4318
Size: 5930
AleI CACnn'nnGTG
Cuts at: 379 379
Size: 5930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 552 709 2731 2824 552
Size: 157 2022 93 3658
Fragments arranged by size:
3658 2022 157 93
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 697 729 697
Size: 32 5898
AlwNI CAG_nnn'CTG
Cuts at: 532 1560 1611 2681 2701 2784 3235 4802 532
Size: 1028 51 1070 20 83 451 1567 1660
Fragments arranged by size:
1660 1567 1070 1028 451 83 51 20
ApaLI G'TGCA_C
Cuts at: 747 1873 4700 747
Size: 1126 2827 1977
Fragments arranged by size:
2827 1977 1126
ApoI r'AATT_y
Cuts at: 2934 5283 5539 2934
Size: 2349 256 3325
Fragments arranged by size:
3325 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5930
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5930
AvaI C'yCGr_G
Cuts at: 1439 1516 1902 2950 1439
Size: 77 386 1048 4419
Fragments arranged by size:
4419 1048 386 77
BamHI G'GATC_C
Cuts at: 507 2825 2955 507
Size: 2318 130 3482
Fragments arranged by size:
3482 2318 130
BanI G'GyrC_C
Cuts at: 1688 2113 2127 2215 2293 2946 3360 3608 3643
Size: 425 14 88 78 653 414 248 35
Cuts at: 3643 1688
Size: 3975
Fragments arranged by size:
3975 653 425 414 248 88 78 35
14
BanII G_rGCy'C
Cuts at: 173 420 1778 1924 2317 2491 2944 3974 173
Size: 247 1358 146 393 174 453 1030 2129
Fragments arranged by size:
2129 1358 1030 453 393 247 174 146
BbeI G_GCGC'C
Cuts at: 1692 3364 3612 1692
Size: 1672 248 4010
Fragments arranged by size:
4010 1672 248
BbsI GAAGACnn'nnnn_
Cuts at: 2544 2544
Size: 5930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 678 712 1275 1309 2103 2137 678
Size: 34 563 34 794 34 4471
Fragments arranged by size:
4471 794 563 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3828 4589 5930 3828
Size: 761 1341 3828
Fragments arranged by size:
3828 1341 761
BclI T'GATC_A
Cuts at: 3450 3450
Size: 5930
BglI GCCn_nnn'nGGC
Cuts at: 364 1150 2269 2312 364
Size: 786 1119 43 3982
Fragments arranged by size:
3982 1119 786 43
BglII A'GATC_T
Cuts at: 149 764 2285 2462 3445 5482 149
Size: 615 1521 177 983 2037 597
Fragments arranged by size:
2037 1521 983 615 597 177
BlpI GC'TnA_GC
Cuts at: 3042 3042
Size: 5930
Bme1580I G_kGCm'C
Cuts at: 255 751 1877 2116 2218 3555 3648 4704 255
Size: 496 1126 239 102 1337 93 1056 1481
Fragments arranged by size:
1481 1337 1126 1056 496 239 102 93
BmrI ACTGGGnnnn_n'
Cuts at: 1835 2020 2658 3142 3223 3558 5702 1835
Size: 185 638 484 81 335 2144 2063
Fragments arranged by size:
2144 2063 638 484 335 185 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4297 4297
Size: 5930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1135 1525 1961 2302 2407 2506 316
Size: 819 390 436 341 105 99 3740
Fragments arranged by size:
3740 819 436 390 341 105 99
Bpu10I CC'TnA_GC
Cuts at: 2273 2273
Size: 5930
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 2902 3106 3985 4477 4775 934
Size: 1968 204 879 492 298 2089
Fragments arranged by size:
2089 1968 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 1594 2902 5634 1594
Size: 1308 2732 1890
Fragments arranged by size:
2732 1890 1308
BsaAI yAC'GTr
Cuts at: 1107 2892 3283 1107
Size: 1785 391 3754
Fragments arranged by size:
3754 1785 391
BsaBI GATnn'nnATC
Cuts at: 6 769 3444 5481 6
Size: 763 2675 2037 455
Fragments arranged by size:
2675 2037 763 455
BsaHI Gr'CG_yC
Cuts at: 1689 1868 3361 3609 4311 5807 1689
Size: 179 1493 248 702 1496 1812
Fragments arranged by size:
1812 1496 1493 702 248 179
BsaWI w'CCGG_w
Cuts at: 485 3119 3640 4318 4592 4739 5150 485
Size: 2634 521 678 274 147 411 1265
Fragments arranged by size:
2634 1265 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 598 628 901 931 1714 1744 598
Size: 30 273 30 783 30 4784
Fragments arranged by size:
4784 783 273 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1993 2043 1993
Size: 50 5880
BseYI C'CCAG_C
Cuts at: 566 682 1992 2259 2842 2885 3355 4113 4690
Size: 116 1310 267 583 43 470 758 577
Cuts at: 4690 5186 566
Size: 496 1310
Fragments arranged by size:
1310 1310 758 583 577 496 470 267
116 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1636 2080 2563 2832 1057
Size: 579 444 483 269 4155
Fragments arranged by size:
4155 579 483 444 269
BsiEI CG_ry'CG
Cuts at: 104 810 3472 3518 4726 104
Size: 706 2662 46 1208 1308
Fragments arranged by size:
2662 1308 1208 706 46
BsiHKAI G_wGCw'C
Cuts at: 173 751 878 1288 1540 1877 1947 2166 2874
Size: 578 127 410 252 337 70 219 708
Cuts at: 2874 2944 3722 3912 4704 173
Size: 70 778 190 792 1399
Fragments arranged by size:
1399 792 778 708 578 410 337 252
219 190 127 70 70
BsmBI CGTCTCn'nnnn_
Cuts at: 1753 1753
Size: 5930
Bsp1286I G_dGCh'C
Cuts at: 173 255 420 751 878 1288 1540 1778 1877
Size: 82 165 331 127 410 252 238 99
Cuts at: 1877 1924 1947 2116 2166 2218 2317 2491 2874
Size: 47 23 169 50 52 99 174 383
Cuts at: 2874 2944 3555 3648 3722 3912 3974 4704 173
Size: 70 611 93 74 190 62 730 1399
Fragments arranged by size:
1399 730 611 410 383 331 252 238
190 174 169 165 127 99 99 93
82 74 70 62 52 50 47 23
BspEI T'CCGG_A
Cuts at: 485 5150 485
Size: 4665 1265
BspHI T'CATG_A
Cuts at: 1130 2360 2495 5106 1130
Size: 1230 135 2611 1954
Fragments arranged by size:
2611 1954 1230 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 701 1611 1670 1997 2339 2346 2980 3194 3496
Size: 910 59 327 342 7 634 214 302
Cuts at: 3496 3877 701
Size: 381 2754
Fragments arranged by size:
2754 910 634 381 342 327 302 214
59 7
BsrBI CCG'CTC
Cuts at: 50 834 2033 4222 4276 5760 5925 50
Size: 784 1199 2189 54 1484 165 55
Fragments arranged by size:
2189 1484 1199 784 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3842 3842
Size: 5930
BsrFI r'CCGG_y
Cuts at: 2612 3928 4109 4318 2612
Size: 1316 181 209 4224
Fragments arranged by size:
4224 1316 209 181
BssSI C'ACGA_G
Cuts at: 4201 4559 4201
Size: 358 5572
BstAPI GCAn_nnn'nTGC
Cuts at: 2701 3307 2701
Size: 606 5324
BstBI TT'CG_AA
Cuts at: 4291 4291
Size: 5930
BstEII G'GTnAC_C
Cuts at: 837 1996 837
Size: 1159 4771
BstXI CCAn_nnnn'nTGG
Cuts at: 636 648 1257 1725 2124 2211 2325 636
Size: 12 609 468 399 87 114 4241
Fragments arranged by size:
4241 609 468 399 114 87 12
BstYI r'GATC_y
Cuts at: 1 149 507 764 1052 2285 2462 2825 2955
Size: 148 358 257 288 1233 177 363 130
Cuts at: 2955 3422 3445 3780 4026 5038 5124 5136 5482
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5482 1
Size: 449
Fragments arranged by size:
1233 1012 467 449 363 358 346 335
288 257 246 177 148 130 86 23
12
BstZ17I GTA'TAC
Cuts at: 315 315
Size: 5930
Bsu36I CC'TnA_GG
Cuts at: 1433 1433
Size: 5930
BtgI C'CryG_G
Cuts at: 233 434 629 1671 1700 2324 233
Size: 201 195 1042 29 624 3839
Fragments arranged by size:
3839 1042 624 201 195 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1143 3880 4061 4224 1143
Size: 2737 181 163 2849
Fragments arranged by size:
2849 2737 181 163
BtsI GCAGTG_nn'
Cuts at: 212 431 653 943 976 2181 2575 3173 212
Size: 219 222 290 33 1205 394 598 2969
Fragments arranged by size:
2969 1205 598 394 290 222 219 33
ClaI AT'CG_AT
Cuts at: 226 2546 226
Size: 2320 3610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1181 1216 1181
Size: 35 5895
DraI TTT'AAA
Cuts at: 2929 2929
Size: 5930
DraIII CAC_nnn'GTG
Cuts at: 755 1403 1405 755
Size: 648 2 5280
Fragments arranged by size:
5280 648 2
DrdI GACnn_nn'nnGTC
Cuts at: 3636 4494 3636
Size: 858 5072
EaeI y'GGCC_r
Cuts at: 356 365 542 638 1239 1634 2045 2528 2627
Size: 9 177 96 601 395 411 483 99
Cuts at: 2627 3515 3689 4080 4107 5452 356
Size: 888 174 391 27 1345 834
Fragments arranged by size:
1345 888 834 601 483 411 395 391
177 174 99 96 27 9
EagI C'GGCC_G
Cuts at: 3515 3515
Size: 5930
EarI CTCTTCn'nnn_
Cuts at: 262 1751 3953 4163 262
Size: 1489 2202 210 2029
Fragments arranged by size:
2202 2029 1489 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1126 2830 4138 4448 4594 5740 1126
Size: 1704 1308 310 146 1146 1316
Fragments arranged by size:
1704 1316 1308 1146 310 146
EcoICRI GAG'CTC
Cuts at: 171 2942 171
Size: 2771 3159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 316 322 517 1078 1135 1525 1775 1961
Size: 135 6 195 561 57 390 250 186
Cuts at: 1961 2302 2407 2506 2544 2806 2881 3755 4187
Size: 341 105 99 38 262 75 874 432
Cuts at: 4187 4934 5202 181
Size: 747 268 909
Fragments arranged by size:
909 874 747 561 432 390 341 268
262 250 195 186 135 105 99 75
57 38 6
EcoO109I rG'GnC_Cy
Cuts at: 396 1623 3069 396
Size: 1227 1446 3257
Fragments arranged by size:
3257 1446 1227
EcoRI G'AATT_C
Cuts at: 2934 2934
Size: 5930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1095 1374 3949 1095
Size: 279 2575 3076
Fragments arranged by size:
3076 2575 279
FspI TGC'GCA
Cuts at: 520 1018 3711 520
Size: 498 2693 2739
Fragments arranged by size:
2739 2693 498
HaeII r_GCGC'y
Cuts at: 658 1278 1692 3364 3612 4634 5613 658
Size: 620 414 1672 248 1022 979 975
Fragments arranged by size:
1672 1022 979 975 620 414 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 598 630 901 933 2153 2185 2493 2525 3772
Size: 32 271 32 1220 32 308 32 1247
Cuts at: 3772 3802 3804 3834 598
Size: 30 2 30 2694
Fragments arranged by size:
2694 1247 1220 308 271 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 273 372 453 1317 2969 273
Size: 99 81 864 1652 3234
Fragments arranged by size:
3234 1652 864 99 81
KasI G'GCGC_C
Cuts at: 1688 3360 3608 1688
Size: 1672 248 4010
Fragments arranged by size:
4010 1672 248
KpnI G_GTAC'C
Cuts at: 2950 2950
Size: 5930
MluI A'CGCG_T
Cuts at: 5363 5363
Size: 5930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 641 2158 4601 4785 641
Size: 1517 2443 184 1786
Fragments arranged by size:
2443 1786 1517 184
MscI TGG'CCA
Cuts at: 358 544 640 1636 2047 2629 3691 358
Size: 186 96 996 411 582 1062 2597
Fragments arranged by size:
2597 1062 996 582 411 186 96
MslI CAynn'nnrTG
Cuts at: 379 634 910 1255 2323 2500 2806 3919 4046
Size: 255 276 345 1068 177 306 1113 127
Cuts at: 4046 379
Size: 2263
Fragments arranged by size:
2263 1113 1068 345 306 276 255 177
127
MspA1I CmG'CkG
Cuts at: 532 570 868 1244 1551 1557 2337 2846 3041
Size: 38 298 376 307 6 780 509 195
Cuts at: 3041 3355 3715 4728 4973 532
Size: 314 360 1013 245 1489
Fragments arranged by size:
1489 1013 780 509 376 360 314 307
298 245 195 38 6
NaeI GCC'GGC
Cuts at: 4111 4111
Size: 5930
NarI GG'CG_CC
Cuts at: 1689 3361 3609 1689
Size: 1672 248 4010
Fragments arranged by size:
4010 1672 248
NcoI C'CATG_G
Cuts at: 434 629 2324 434
Size: 195 1695 4040
Fragments arranged by size:
4040 1695 195
NdeI CA'TA_TG
Cuts at: 513 2232 513
Size: 1719 4211
NgoMIV G'CCGG_C
Cuts at: 4109 4109
Size: 5930
NspI r_CATG'y
Cuts at: 282 2772 2983 3483 4390 282
Size: 2490 211 500 907 1822
Fragments arranged by size:
2490 1822 907 500 211
PasI CC'CwG_GG
Cuts at: 1980 1980
Size: 5930
PciI A'CATG_T
Cuts at: 4386 4386
Size: 5930
PflMI CCAn_nnn'nTGG
Cuts at: 573 1170 1454 1611 2457 573
Size: 597 284 157 846 4046
Fragments arranged by size:
4046 846 597 284 157
PfoI T'CCnGG_A
Cuts at: 590 773 1328 590
Size: 183 555 5192
Fragments arranged by size:
5192 555 183
PmeI GTTT'AAAC
Cuts at: 2929 2929
Size: 5930
PmlI CAC'GTG
Cuts at: 2892 2892
Size: 5930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2682 5069 5101 2650
Size: 32 2387 32 3479
Fragments arranged by size:
3479 2387 32 32
PpuMI rG'GwC_Cy
Cuts at: 396 1623 396
Size: 1227 4703
PshAI GACnn'nnGTC
Cuts at: 1312 1312
Size: 5930
PspXI vC'TCGA_Gb
Cuts at: 1516 1902 1516
Size: 386 5544
PstI C_TGCA'G
Cuts at: 1004 1556 2336 2720 2977 3662 1004
Size: 552 780 384 257 685 3272
Fragments arranged by size:
3272 780 685 552 384 257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5930
PvuII CAG'CTG
Cuts at: 532 570 1551 1557 2337 2846 3355 3715 532
Size: 38 981 6 780 509 509 360 2747
Fragments arranged by size:
2747 981 780 509 509 360 38 6
RsrII CG'GwC_CG
Cuts at: 4125 4125
Size: 5930
SacI G_AGCT'C
Cuts at: 173 2944 173
Size: 2771 3159
SalI G'TCGA_C
Cuts at: 2967 2967
Size: 5930
SapI GCTCTTCn'nnn_
Cuts at: 3953 4163 3953
Size: 210 5720
SbfI CC_TGCA'GG
Cuts at: 2977 2977
Size: 5930
ScaI AGT'ACT
Cuts at: 5447 5447
Size: 5930
SfcI C'TryA_G
Cuts at: 33 1000 1552 2332 2716 2973 3658 4651 4842
Size: 967 552 780 384 257 685 993 191
Cuts at: 4842 33
Size: 1121
Fragments arranged by size:
1121 993 967 780 685 552 384 257
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 364 1150 364
Size: 786 5144
SfoI GGC'GCC
Cuts at: 1690 3362 3610 1690
Size: 1672 248 4010
Fragments arranged by size:
4010 1672 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5930
SmaI CCC'GGG
Cuts at: 1441 2952 1441
Size: 1511 4419
SmlI C'TyrA_G
Cuts at: 949 1516 1902 2917 3085 4000 4492 4754 949
Size: 567 386 1015 168 915 492 262 2125
Fragments arranged by size:
2125 1015 915 567 492 386 262 168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5930
SphI G_CATG'C
Cuts at: 282 2983 3483 282
Size: 2701 500 2729
Fragments arranged by size:
2729 2701 500
SrfI GCCC'GGGC
Cuts at: 1441 1441
Size: 5930
SspI AAT'ATT
Cuts at: 124 124
Size: 5930
StuI AGG'CCT
Cuts at: 1047 2350 2902 1047
Size: 1303 552 4075
Fragments arranged by size:
4075 1303 552
StyI C'CwwG_G
Cuts at: 434 629 800 1310 1718 2324 3064 3418 434
Size: 195 171 510 408 606 740 354 2946
Fragments arranged by size:
2946 740 606 510 408 354 195 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1984 2181 2380 2481 2796 3513 3646 4105 4314
Size: 197 199 101 315 717 133 459 209
Cuts at: 4314 1984
Size: 3600
Fragments arranged by size:
3600 717 459 315 209 199 197 133
101
TatI w'GTAC_w
Cuts at: 1088 5445 1088
Size: 4357 1573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2219 2251 2567 2599 5447 5479 2219
Size: 32 316 32 2848 32 2670
Fragments arranged by size:
2848 2670 316 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3727 3727
Size: 5930
XbaI T'CTAG_A
Cuts at: 2961 2961
Size: 5930
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 867 1707 1848 2454 2751 3425 867
Size: 840 141 606 297 674 3372
Fragments arranged by size:
3372 840 674 606 297 141
XhoI C'TCGA_G
Cuts at: 1516 1902 1516
Size: 386 5544
XmaI C'CCGG_G
Cuts at: 1439 2950 1439
Size: 1511 4419
XmnI GAAnn'nnTTC
Cuts at: 1075 1075
Size: 5930
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI ApaI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PsiI PspOMI PsrI SacII
SanDI SexAI SgrAI SnaBI SwaI ZraI