Restriction map for
pF1KB4096
(Circular) (Six-base) MAPSORT of: pF1KB4096.seq Check: 6049 from: 1 to: 6032
pF1KB4096 6032 bp
With 182 enzymes: SgfI *
January 12, 2016 10:05 ..
AccI GT'mk_AC
Cuts at: 668 2175 3070 668
Size: 1507 895 3630
Fragments arranged by size:
3630 1507 895
Acc65I G'GTAC_C
Cuts at: 3048 3048
Size: 6032
AclI AA'CG_TT
Cuts at: 5872 5872
Size: 6032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1483 1807 3857 4289 5036 5304 247
Size: 1236 324 2050 432 747 268 975
Fragments arranged by size:
2050 1236 975 747 432 324 268
AflIII A'CryG_T
Cuts at: 3384 4488 5465 3384
Size: 1104 977 3951
Fragments arranged by size:
3951 1104 977
AgeI A'CCGG_T
Cuts at: 4420 4420
Size: 6032
AleI CACnn'nnGTG
Cuts at: 1873 1873
Size: 6032
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 181 181
Size: 6032
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 496 528 799 831 496
Size: 32 271 32 5697
Fragments arranged by size:
5697 271 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2903 3337 4904 2903
Size: 434 1567 4031
Fragments arranged by size:
4031 1567 434
ApaLI G'TGCA_C
Cuts at: 4802 4802
Size: 6032
ApoI r'AATT_y
Cuts at: 520 1663 1891 2262 2298 2331 2659 2913 3036
Size: 1143 228 371 36 33 328 254 123
Cuts at: 3036 5385 5641 520
Size: 2349 256 911
Fragments arranged by size:
2349 1143 911 371 328 256 254 228
123 36 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 6032
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6032
AvaI C'yCGr_G
Cuts at: 2477 3052 2477
Size: 575 5457
BamHI G'GATC_C
Cuts at: 1772 3057 1772
Size: 1285 4747
BanI G'GyrC_C
Cuts at: 1523 2071 2572 3048 3462 3710 3745 1523
Size: 548 501 476 414 248 35 3810
Fragments arranged by size:
3810 548 501 476 414 248 35
BanII G_rGCy'C
Cuts at: 194 788 797 839 892 2478 3046 4076 194
Size: 594 9 42 53 1586 568 1030 2150
Fragments arranged by size:
2150 1586 1030 594 568 53 42 9
BbeI G_GCGC'C
Cuts at: 3466 3714 3466
Size: 248 5784
BciVI GTATCCnnnnn_n'
Cuts at: 3930 4691 6032 3930
Size: 761 1341 3930
Fragments arranged by size:
3930 1341 761
BclI T'GATC_A
Cuts at: 3552 3552
Size: 6032
BfrBI ATG'CAT
Cuts at: 403 403
Size: 6032
BglII A'GATC_T
Cuts at: 275 1628 3547 5584 275
Size: 1353 1919 2037 723
Fragments arranged by size:
2037 1919 1353 723
BlpI GC'TnA_GC
Cuts at: 454 796 1016 3144 454
Size: 342 220 2128 3342
Fragments arranged by size:
3342 2128 342 220
Bme1580I G_kGCm'C
Cuts at: 327 2577 3657 3750 4806 327
Size: 2250 1080 93 1056 1553
Fragments arranged by size:
2250 1553 1080 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1095 2096 2650 2678 3244 3325 3660 5804 1095
Size: 1001 554 28 566 81 335 2144 1323
Fragments arranged by size:
2144 1323 1001 566 554 335 81 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 855 4399 855
Size: 3544 2488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 876 922 2343 376
Size: 500 46 1421 4065
Fragments arranged by size:
4065 1421 500 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 2214 2617 3208 4087 4579 4877 772
Size: 1442 403 591 879 492 298 1927
Fragments arranged by size:
1927 1442 879 591 492 403 298
BsaI GGTCTCn'nnnn_
Cuts at: 146 2156 2650 5736 146
Size: 2010 494 3086 442
Fragments arranged by size:
3086 2010 494 442
BsaAI yAC'GTr
Cuts at: 3385 3385
Size: 6032
BsaBI GATnn'nnATC
Cuts at: 6 3546 5583 6
Size: 3540 2037 455
Fragments arranged by size:
3540 2037 455
BsaHI Gr'CG_yC
Cuts at: 3463 3711 4413 5909 3463
Size: 248 702 1496 3586
Fragments arranged by size:
3586 1496 702 248
BsaWI w'CCGG_w
Cuts at: 528 3221 3742 4420 4694 4841 5252 528
Size: 2693 521 678 274 147 411 1308
Fragments arranged by size:
2693 1308 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 637 667 673 703 799 829 2630 2660 637
Size: 30 6 30 96 30 1801 30 4009
Fragments arranged by size:
4009 1801 96 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 847 2898 2922 217
Size: 630 2051 24 3327
Fragments arranged by size:
3327 2051 630 24
BseYI C'CCAG_C
Cuts at: 469 779 3457 4215 4792 5288 469
Size: 310 2678 758 577 496 1213
Fragments arranged by size:
2678 1213 758 577 496 310
BsiEI CG_ry'CG
Cuts at: 104 3574 3620 4828 104
Size: 3470 46 1208 1308
Fragments arranged by size:
3470 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 194 788 797 839 892 3046 3824 4014 4806
Size: 594 9 42 53 2154 778 190 792
Cuts at: 4806 194
Size: 1420
Fragments arranged by size:
2154 1420 792 778 594 190 53 42
9
BsmI GAATG_Cn'
Cuts at: 2863 2863
Size: 6032
BsmBI CGTCTCn'nnnn_
Cuts at: 1588 2441 1588
Size: 853 5179
Bsp1286I G_dGCh'C
Cuts at: 194 327 788 797 839 892 2478 2577 3046
Size: 133 461 9 42 53 1586 99 469
Cuts at: 3046 3657 3750 3824 4014 4076 4806 194
Size: 611 93 74 190 62 730 1420
Fragments arranged by size:
1586 1420 730 611 469 461 190 133
99 93 74 62 53 42 9
BspEI T'CCGG_A
Cuts at: 5252 5252
Size: 6032
BspHI T'CATG_A
Cuts at: 5208 5208
Size: 6032
BspMI ACCTGCnnnn'nnnn_
Cuts at: 474 878 1114 3082 3296 3598 3979 474
Size: 404 236 1968 214 302 381 2527
Fragments arranged by size:
2527 1968 404 381 302 236 214
BsrBI CCG'CTC
Cuts at: 50 4324 4378 5862 6027 50
Size: 4274 54 1484 165 55
Fragments arranged by size:
4274 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 332 403 731 1436 2220 3944 332
Size: 71 328 705 784 1724 2420
Fragments arranged by size:
2420 1724 784 705 328 71
BsrFI r'CCGG_y
Cuts at: 4030 4211 4420 4030
Size: 181 209 5642
Fragments arranged by size:
5642 209 181
BsrGI T'GTAC_A
Cuts at: 1290 2699 1290
Size: 1409 4623
BssSI C'ACGA_G
Cuts at: 4303 4661 4303
Size: 358 5674
BstAPI GCAn_nnn'nTGC
Cuts at: 2858 3409 2858
Size: 551 5481
BstBI TT'CG_AA
Cuts at: 4393 4393
Size: 6032
BstXI CCAn_nnnn'nTGG
Cuts at: 549 900 549
Size: 351 5681
BstYI r'GATC_y
Cuts at: 1 275 626 1628 1772 3057 3524 3547 3882
Size: 274 351 1002 144 1285 467 23 335
Cuts at: 3882 4128 5140 5226 5238 5584 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1285 1012 1002 467 449 351 346 335
274 246 144 86 23 12
BtgI C'CryG_G
Cuts at: 107 613 2532 107
Size: 506 1919 3607
Fragments arranged by size:
3607 1919 506
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3982 4163 4326 3982
Size: 181 163 5688
Fragments arranged by size:
5688 181 163
BtsI GCAGTG_nn'
Cuts at: 853 2472 2859 3275 853
Size: 1619 387 416 3610
Fragments arranged by size:
3610 1619 416 387
ClaI AT'CG_AT
Cuts at: 1748 1748
Size: 6032
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1295 1330 1794 1829 1295
Size: 35 464 35 5498
Fragments arranged by size:
5498 464 35 35
DraI TTT'AAA
Cuts at: 1856 3031 1856
Size: 1175 4857
DraIII CAC_nnn'GTG
Cuts at: 387 1049 387
Size: 662 5370
DrdI GACnn_nn'nnGTC
Cuts at: 1824 3738 4596 1824
Size: 1914 858 3260
Fragments arranged by size:
3260 1914 858
EaeI y'GGCC_r
Cuts at: 968 3617 3791 4182 4209 5554 968
Size: 2649 174 391 27 1345 1446
Fragments arranged by size:
2649 1446 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3617 3617
Size: 6032
EarI CTCTTCn'nnn_
Cuts at: 932 1555 1826 1837 2888 4055 4265 932
Size: 623 271 11 1051 1167 210 2699
Fragments arranged by size:
2699 1167 1051 623 271 210 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1092 4240 4550 4696 5842 1092
Size: 3148 310 146 1146 1282
Fragments arranged by size:
3148 1282 1146 310 146
EcoICRI GAG'CTC
Cuts at: 192 786 795 837 890 3044 192
Size: 594 9 42 53 2154 3180
Fragments arranged by size:
3180 2154 594 53 42 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 376 876 922 1483 1807 2343 3857 4289
Size: 129 500 46 561 324 536 1514 432
Cuts at: 4289 5036 5304 247
Size: 747 268 975
Fragments arranged by size:
1514 975 747 561 536 500 432 324
268 129 46
EcoNI CCTnn'n_nnAGG
Cuts at: 265 2812 265
Size: 2547 3485
EcoO109I rG'GnC_Cy
Cuts at: 258 486 750 1297 1618 2623 3171 258
Size: 228 264 547 321 1005 548 3119
Fragments arranged by size:
3119 1005 548 547 321 264 228
EcoRI G'AATT_C
Cuts at: 520 1891 3036 520
Size: 1371 1145 3516
Fragments arranged by size:
3516 1371 1145
EcoRV GAT'ATC
Cuts at: 1656 2016 1656
Size: 360 5672
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1212 4051 1212
Size: 2839 3193
FspI TGC'GCA
Cuts at: 3813 3813
Size: 6032
HaeII r_GCGC'y
Cuts at: 440 2843 3466 3714 4736 5715 440
Size: 2403 623 248 1022 979 757
Fragments arranged by size:
2403 1022 979 757 623 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 143 175 637 669 686 718 2220 2252 3874
Size: 32 462 32 17 32 1502 32 1622
Cuts at: 3874 3904 3906 3936 143
Size: 30 2 30 2239
Fragments arranged by size:
2239 1622 1502 462 32 32 32 32
30 30 17 2
HincII GTy'rAC
Cuts at: 135 291 3071 135
Size: 156 2780 3096
Fragments arranged by size:
3096 2780 156
HindIII A'AGCT_T
Cuts at: 1096 1096
Size: 6032
HpaI GTT'AAC
Cuts at: 291 291
Size: 6032
KasI G'GCGC_C
Cuts at: 3462 3710 3462
Size: 248 5784
KpnI G_GTAC'C
Cuts at: 3052 3052
Size: 6032
MluI A'CGCG_T
Cuts at: 5465 5465
Size: 6032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1586 4703 4887 1586
Size: 3117 184 2731
Fragments arranged by size:
3117 2731 184
MscI TGG'CCA
Cuts at: 970 3793 970
Size: 2823 3209
MslI CAynn'nnrTG
Cuts at: 496 923 1873 4021 4148 496
Size: 427 950 2148 127 2380
Fragments arranged by size:
2380 2148 950 427 127
MspA1I CmG'CkG
Cuts at: 473 632 3143 3457 3817 4830 5075 473
Size: 159 2511 314 360 1013 245 1430
Fragments arranged by size:
2511 1430 1013 360 314 245 159
NaeI GCC'GGC
Cuts at: 4213 4213
Size: 6032
NarI GG'CG_CC
Cuts at: 3463 3711 3463
Size: 248 5784
NcoI C'CATG_G
Cuts at: 107 2532 107
Size: 2425 3607
NdeI CA'TA_TG
Cuts at: 2672 2672
Size: 6032
NgoMIV G'CCGG_C
Cuts at: 4211 4211
Size: 6032
NsiI A_TGCA'T
Cuts at: 405 405
Size: 6032
NspI r_CATG'y
Cuts at: 1647 1965 3085 3585 4492 1647
Size: 318 1120 500 907 3187
Fragments arranged by size:
3187 1120 907 500 318
PciI A'CATG_T
Cuts at: 4488 4488
Size: 6032
PflMI CCAn_nnn'nTGG
Cuts at: 966 1049 966
Size: 83 5949
PmeI GTTT'AAAC
Cuts at: 3031 3031
Size: 6032
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 799 831 5171 5203 799
Size: 32 4340 32 1628
Fragments arranged by size:
4340 1628 32 32
PpuMI rG'GwC_Cy
Cuts at: 486 750 1618 2623 486
Size: 264 868 1005 3895
Fragments arranged by size:
3895 1005 868 264
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1426 1458 1705 1737 1426
Size: 32 247 32 5721
Fragments arranged by size:
5721 247 32 32
PstI C_TGCA'G
Cuts at: 201 1628 3079 3764 201
Size: 1427 1451 685 2469
Fragments arranged by size:
2469 1451 1427 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6032
PvuII CAG'CTG
Cuts at: 3457 3817 3457
Size: 360 5672
RsrII CG'GwC_CG
Cuts at: 4227 4227
Size: 6032
SacI G_AGCT'C
Cuts at: 194 788 797 839 892 3046 194
Size: 594 9 42 53 2154 3180
Fragments arranged by size:
3180 2154 594 53 42 9
SalI G'TCGA_C
Cuts at: 3069 3069
Size: 6032
SapI GCTCTTCn'nnn_
Cuts at: 932 4055 4265 932
Size: 3123 210 2699
Fragments arranged by size:
3123 2699 210
SbfI CC_TGCA'GG
Cuts at: 3079 3079
Size: 6032
ScaI AGT'ACT
Cuts at: 5549 5549
Size: 6032
SfcI C'TryA_G
Cuts at: 33 197 459 1142 1624 3075 3760 4753 4944
Size: 164 262 683 482 1451 685 993 191
Cuts at: 4944 33
Size: 1121
Fragments arranged by size:
1451 1121 993 685 683 482 262 191
164
SfoI GGC'GCC
Cuts at: 3464 3712 3464
Size: 248 5784
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6032
SmaI CCC'GGG
Cuts at: 3054 3054
Size: 6032
SmlI C'TyrA_G
Cuts at: 787 2193 2632 3187 4102 4594 4856 787
Size: 1406 439 555 915 492 262 1963
Fragments arranged by size:
1963 1406 915 555 492 439 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6032
SphI G_CATG'C
Cuts at: 1647 3085 3585 1647
Size: 1438 500 4094
Fragments arranged by size:
4094 1438 500
StuI AGG'CCT
Cuts at: 211 2785 211
Size: 2574 3458
StyI C'CwwG_G
Cuts at: 107 311 1173 1333 1501 2124 2407 2532 2560
Size: 204 862 160 168 623 283 125 28
Cuts at: 2560 2577 3166 3520 107
Size: 17 589 354 2619
Fragments arranged by size:
2619 862 623 589 354 283 204 168
160 125 28 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 385 1176 3615 3748 4207 4416 385
Size: 791 2439 133 459 209 2001
Fragments arranged by size:
2439 2001 791 459 209 133
TatI w'GTAC_w
Cuts at: 119 1290 2699 5547 119
Size: 1171 1409 2848 604
Fragments arranged by size:
2848 1409 1171 604
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 673 705 1207 1239 1764 1796 2878 2910 5549
Size: 32 502 32 525 32 1082 32 2639
Cuts at: 5549 5581 673
Size: 32 1124
Fragments arranged by size:
2639 1124 1082 525 502 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3829 3829
Size: 6032
XbaI T'CTAG_A
Cuts at: 3063 3063
Size: 6032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 498 870 978 1508 3527 498
Size: 372 108 530 2019 3003
Fragments arranged by size:
3003 2019 530 372 108
XmaI C'CCGG_G
Cuts at: 3052 3052
Size: 6032
XmnI GAAnn'nnTTC
Cuts at: 211 814 211
Size: 603 5429
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BssHII BstEII BstZ17I Bsu36I FseI FspAI
MfeI NheI NotI NruI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI