Restriction map for
pF1KB4088
(Circular) (Six-base) MAPSORT of: pF1KB4088.seq Check: 4541 from: 1 to: 5393
pF1KB4088 5393 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 729 1615 2431 729
Size: 886 816 3691
Fragments arranged by size:
3691 886 816
Acc65I G'GTAC_C
Cuts at: 1576 1807 2409 1576
Size: 231 602 4560
Fragments arranged by size:
4560 602 231
AclI AA'CG_TT
Cuts at: 5233 5233
Size: 5393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 3218 3650 4003 4665 508
Size: 2710 432 353 662 1236
Fragments arranged by size:
2710 1236 662 432 353
AfeI AGC'GCT
Cuts at: 693 693
Size: 5393
AflIII A'CryG_T
Cuts at: 1192 2202 2745 4545 4826 1192
Size: 1010 543 1800 281 1759
Fragments arranged by size:
1800 1759 1010 543 281
AleI CACnn'nnGTG
Cuts at: 853 853
Size: 5393
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 408 440 408
Size: 32 5361
AlwNI CAG_nnn'CTG
Cuts at: 793 1688 2031 2698 4136 793
Size: 895 343 667 1438 2050
Fragments arranged by size:
2050 1438 895 667 343
ApaI G_GGCC'C
Cuts at: 1329 1348 1329
Size: 19 5374
ApaLI G'TGCA_C
Cuts at: 4231 4231
Size: 5393
ApoI r'AATT_y
Cuts at: 555 631 967 1262 2397 4746 5002 555
Size: 76 336 295 1135 2349 256 946
Fragments arranged by size:
2349 1135 946 336 295 256 76
AseI AT'TA_AT
Cuts at: 20 1553 20
Size: 1533 3860
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5393
AvaI C'yCGr_G
Cuts at: 2413 2413
Size: 5393
BamHI G'GATC_C
Cuts at: 2418 2418
Size: 5393
BanI G'GyrC_C
Cuts at: 532 1492 1576 1807 2409 2823 3071 3106 532
Size: 960 84 231 602 414 248 35 2819
Fragments arranged by size:
2819 960 602 414 248 231 84 35
BanII G_rGCy'C
Cuts at: 1329 1348 2218 2407 3437 1329
Size: 19 870 189 1030 3285
Fragments arranged by size:
3285 1030 870 189 19
BbeI G_GCGC'C
Cuts at: 2827 3075 2827
Size: 248 5145
BbsI GAAGACnn'nnnn_
Cuts at: 2199 2199
Size: 5393
BciVI GTATCCnnnnn_n'
Cuts at: 1507 3291 4347 5393 1507
Size: 1784 1056 1046 1507
Fragments arranged by size:
1784 1507 1056 1046
BclI T'GATC_A
Cuts at: 657 707 2913 657
Size: 50 2206 3137
Fragments arranged by size:
3137 2206 50
BfrBI ATG'CAT
Cuts at: 1339 1339
Size: 5393
BglII A'GATC_T
Cuts at: 2908 4945 2908
Size: 2037 3356
BlpI GC'TnA_GC
Cuts at: 2505 2505
Size: 5393
Bme1580I G_kGCm'C
Cuts at: 1329 1348 3018 3111 4235 1329
Size: 19 1670 93 1124 2487
Fragments arranged by size:
2487 1670 1124 93 19
BmgBI CAC'GTC
Cuts at: 466 466
Size: 5393
BmrI ACTGGGnnnn_n'
Cuts at: 1670 1698 2605 2686 3021 5165 1670
Size: 28 907 81 335 2144 1898
Fragments arranged by size:
2144 1898 907 335 81 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3760 3760
Size: 5393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 1104 1370 1493 2266 410
Size: 694 266 123 773 3537
Fragments arranged by size:
3537 773 694 266 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2569 3448 4162 4460 2569
Size: 879 714 298 3502
Fragments arranged by size:
3502 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5097 5097
Size: 5393
BsaAI yAC'GTr
Cuts at: 2175 2746 2175
Size: 571 4822
BsaBI GATnn'nnATC
Cuts at: 6 596 2907 4944 6
Size: 590 2311 2037 455
Fragments arranged by size:
2311 2037 590 455
BsaHI Gr'CG_yC
Cuts at: 2824 3072 3774 5270 2824
Size: 248 702 1496 2947
Fragments arranged by size:
2947 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1363 1653 2582 3103 3781 4192 4339 4613 1363
Size: 290 929 521 678 411 147 274 2143
Fragments arranged by size:
2143 929 678 521 411 290 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 408 438 408
Size: 30 5363
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 451
Size: 5393
BseYI C'CCAG_C
Cuts at: 1438 1758 2818 3576 4241 4649 1438
Size: 320 1060 758 665 408 2182
Fragments arranged by size:
2182 1060 758 665 408 320
BsiEI CG_ry'CG
Cuts at: 104 2935 2981 4211 104
Size: 2831 46 1230 1286
Fragments arranged by size:
2831 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2407 3185 3375 4235 2407
Size: 778 190 860 3565
Fragments arranged by size:
3565 860 778 190
BsmI GAATG_Cn'
Cuts at: 130 2097 130
Size: 1967 3426
Bsp1286I G_dGCh'C
Cuts at: 1329 1348 2218 2407 3018 3111 3185 3375 3437
Size: 19 870 189 611 93 74 190 62
Cuts at: 3437 4235 1329
Size: 798 2487
Fragments arranged by size:
2487 870 798 611 190 189 93 74
62 19
BspHI T'CATG_A
Cuts at: 2117 2141 3825 2117
Size: 24 1684 3685
Fragments arranged by size:
3685 1684 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2443 2657 2959 3340 2443
Size: 214 302 381 4496
Fragments arranged by size:
4496 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3685 3739 5223 5388 50
Size: 3635 54 1484 165 55
Fragments arranged by size:
3635 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1248 3305 1248
Size: 2057 3336
BsrFI r'CCGG_y
Cuts at: 529 3391 3572 529
Size: 2862 181 2350
Fragments arranged by size:
2862 2350 181
BssSI C'ACGA_G
Cuts at: 569 1868 2042 3664 4372 569
Size: 1299 174 1622 708 1590
Fragments arranged by size:
1622 1590 1299 708 174
BstAPI GCAn_nnn'nTGC
Cuts at: 2770 2770
Size: 5393
BstBI TT'CG_AA
Cuts at: 3754 3754
Size: 5393
BstEII G'GTnAC_C
Cuts at: 1024 1203 1024
Size: 179 5214
BstYI r'GATC_y
Cuts at: 1 580 862 1977 2418 2885 2908 3243 3489
Size: 579 282 1115 441 467 23 335 246
Cuts at: 3489 3795 3807 3893 4945 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1115 1052 579 467 449 441 335 306
282 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1616 1616
Size: 5393
BtgI C'CryG_G
Cuts at: 1814 1814
Size: 5393
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3343 3524 3687 3343
Size: 181 163 5049
Fragments arranged by size:
5049 181 163
BtsI GCAGTG_nn'
Cuts at: 2636 2636
Size: 5393
DraI TTT'AAA
Cuts at: 636 1535 2392 636
Size: 899 857 3637
Fragments arranged by size:
3637 899 857
DrdI GACnn_nn'nnGTC
Cuts at: 2113 3099 4443 2113
Size: 986 1344 3063
Fragments arranged by size:
3063 1344 986
EaeI y'GGCC_r
Cuts at: 2978 3152 3543 3570 4915 2978
Size: 174 391 27 1345 3456
Fragments arranged by size:
3456 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2978 2978
Size: 5393
EarI CTCTTCn'nnn_
Cuts at: 484 3416 3626 484
Size: 2932 210 2251
Fragments arranged by size:
2932 2251 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3601 4343 4489 5203 3601
Size: 742 146 714 3791
Fragments arranged by size:
3791 742 714 146
EcoICRI GAG'CTC
Cuts at: 2405 2405
Size: 5393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 508 1104 1370 1493 2266 3218 3650 4003
Size: 98 596 266 123 773 952 432 353
Cuts at: 4003 4665 410
Size: 662 1138
Fragments arranged by size:
1138 952 773 662 596 432 353 266
123 98
EcoO109I rG'GnC_Cy
Cuts at: 1325 1344 1345 2532 1325
Size: 19 1 1187 4186
Fragments arranged by size:
4186 1187 19 1
EcoRI G'AATT_C
Cuts at: 2397 2397
Size: 5393
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1588 3412 1588
Size: 1824 3569
FspI TGC'GCA
Cuts at: 3174 3174
Size: 5393
HaeII r_GCGC'y
Cuts at: 695 2827 3075 4305 5076 695
Size: 2132 248 1230 771 1012
Fragments arranged by size:
2132 1230 1012 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 565 597 1153 1185 1468 1500 3235 3265 3267
Size: 32 556 32 283 32 1735 30 2
Cuts at: 3267 3297 565
Size: 30 2661
Fragments arranged by size:
2661 1735 556 283 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 981 2432 981
Size: 1451 3942
HindIII A'AGCT_T
Cuts at: 924 924
Size: 5393
HpaI GTT'AAC
Cuts at: 981 981
Size: 5393
KasI G'GCGC_C
Cuts at: 2823 3071 2823
Size: 248 5145
KpnI G_GTAC'C
Cuts at: 1580 1811 2413 1580
Size: 231 602 4560
Fragments arranged by size:
4560 602 231
MfeI C'AATT_G
Cuts at: 935 935
Size: 5393
MluI A'CGCG_T
Cuts at: 4826 4826
Size: 5393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 482 1444 2223 4152 4336 482
Size: 962 779 1929 184 1539
Fragments arranged by size:
1929 1539 962 779 184
MscI TGG'CCA
Cuts at: 3154 3154
Size: 5393
MslI CAynn'nnrTG
Cuts at: 853 2146 3382 3509 853
Size: 1293 1236 127 2737
Fragments arranged by size:
2737 1293 1236 127
MspA1I CmG'CkG
Cuts at: 1876 2504 2818 3178 3962 4207 1876
Size: 628 314 360 784 245 3062
Fragments arranged by size:
3062 784 628 360 314 245
NaeI GCC'GGC
Cuts at: 3574 3574
Size: 5393
NarI GG'CG_CC
Cuts at: 2824 3072 2824
Size: 248 5145
NcoI C'CATG_G
Cuts at: 1814 1814
Size: 5393
NdeI CA'TA_TG
Cuts at: 561 561
Size: 5393
NgoMIV G'CCGG_C
Cuts at: 3572 3572
Size: 5393
NsiI A_TGCA'T
Cuts at: 1341 1341
Size: 5393
NspI r_CATG'y
Cuts at: 1009 1196 2206 2446 2946 4549 1009
Size: 187 1010 240 500 1603 1853
Fragments arranged by size:
1853 1603 1010 500 240 187
PciI A'CATG_T
Cuts at: 1192 2202 4545 1192
Size: 1010 2343 2040
Fragments arranged by size:
2343 2040 1010
PflMI CCAn_nnn'nTGG
Cuts at: 1502 1502
Size: 5393
PmeI GTTT'AAAC
Cuts at: 2392 2392
Size: 5393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 408 440 3839 3871 408
Size: 32 3399 32 1930
Fragments arranged by size:
3399 1930 32 32
PspOMI G'GGCC_C
Cuts at: 1325 1344 1325
Size: 19 5374
PstI C_TGCA'G
Cuts at: 462 1134 1881 2440 3125 462
Size: 672 747 559 685 2730
Fragments arranged by size:
2730 747 685 672 559
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5393
PvuII CAG'CTG
Cuts at: 1876 2818 3178 1876
Size: 942 360 4091
Fragments arranged by size:
4091 942 360
RsrII CG'GwC_CG
Cuts at: 3588 3588
Size: 5393
SacI G_AGCT'C
Cuts at: 2407 2407
Size: 5393
SalI G'TCGA_C
Cuts at: 2430 2430
Size: 5393
SapI GCTCTTCn'nnn_
Cuts at: 3416 3626 3416
Size: 210 5183
SbfI CC_TGCA'GG
Cuts at: 2440 2440
Size: 5393
ScaI AGT'ACT
Cuts at: 4910 4910
Size: 5393
SexAI A'CCwGG_T
Cuts at: 740 740
Size: 5393
SfcI C'TryA_G
Cuts at: 33 230 458 866 1130 1877 2436 3121 4089
Size: 197 228 408 264 747 559 685 968
Cuts at: 4089 4280 33
Size: 191 1146
Fragments arranged by size:
1146 968 747 685 559 408 264 228
197 191
SfoI GGC'GCC
Cuts at: 2825 3073 2825
Size: 248 5145
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5393
SmaI CCC'GGG
Cuts at: 2415 2415
Size: 5393
SmlI C'TyrA_G
Cuts at: 2548 3463 4177 4439 2548
Size: 915 714 262 3502
Fragments arranged by size:
3502 915 714 262
SnaBI TAC'GTA
Cuts at: 2175 2175
Size: 5393
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5393
SphI G_CATG'C
Cuts at: 2446 2946 2446
Size: 500 4893
StyI C'CwwG_G
Cuts at: 1814 2527 2881 1814
Size: 713 354 4326
Fragments arranged by size:
4326 713 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1070 2976 3109 3568 3777 1070
Size: 1906 133 459 209 2686
Fragments arranged by size:
2686 1906 459 209 133
TatI w'GTAC_w
Cuts at: 4908 4908
Size: 5393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 854 886 1028 1053 1060 1085 1779 1811 4910
Size: 32 142 25 7 25 694 32 3099
Cuts at: 4910 4942 854
Size: 32 1305
Fragments arranged by size:
3099 1305 694 142 32 32 32 25
25 7
Tth111I GACn'n_nGTC
Cuts at: 3190 3190
Size: 5393
XbaI T'CTAG_A
Cuts at: 2424 2424
Size: 5393
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 814 2888 814
Size: 2074 3319
XmaI C'CCGG_G
Cuts at: 2413 2413
Size: 5393
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AlfI AscI AvrII
BaeI BbvCI BcgI BglI BmtI Bpu10I BsgI BsiWI
BsmBI BspEI BsrGI BssHII BstXI Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI NheI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI ZraI