Restriction map for
pF1KB4080
(Circular) (Six-base) MAPSORT of: pF1KB4080.seq Check: 4796 from: 1 to: 4127
pF1KB4080 4127 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 783 1165 783
Size: 382 3745
Acc65I G'GTAC_C
Cuts at: 721 1143 721
Size: 422 3705
AclI AA'CG_TT
Cuts at: 3967 3967
Size: 4127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1952 2384 3131 3399 1952
Size: 432 747 268 2680
Fragments arranged by size:
2680 747 432 268
AfeI AGC'GCT
Cuts at: 412 412
Size: 4127
AflIII A'CryG_T
Cuts at: 191 245 1479 2583 3560 191
Size: 54 1234 1104 977 758
Fragments arranged by size:
1234 1104 977 758 54
AgeI A'CCGG_T
Cuts at: 2515 2515
Size: 4127
AlwNI CAG_nnn'CTG
Cuts at: 1432 2999 1432
Size: 1567 2560
ApaLI G'TGCA_C
Cuts at: 2897 2897
Size: 4127
ApoI r'AATT_y
Cuts at: 349 611 620 733 884 1023 1131 3480 3736
Size: 262 9 113 151 139 108 2349 256
Cuts at: 3736 349
Size: 740
Fragments arranged by size:
2349 740 262 256 151 139 113 108
9
AseI AT'TA_AT
Cuts at: 20 698 20
Size: 678 3449
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
AvaI C'yCGr_G
Cuts at: 947 1147 947
Size: 200 3927
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 497 530 497
Size: 33 4094
BamHI G'GATC_C
Cuts at: 1152 1152
Size: 4127
BanI G'GyrC_C
Cuts at: 721 1143 1557 1805 1840 721
Size: 422 414 248 35 3008
Fragments arranged by size:
3008 422 414 248 35
BanII G_rGCy'C
Cuts at: 429 1141 2171 429
Size: 712 1030 2385
Fragments arranged by size:
2385 1030 712
BbeI G_GCGC'C
Cuts at: 1561 1809 1561
Size: 248 3879
BbsI GAAGACnn'nnnn_
Cuts at: 589 589
Size: 4127
BciVI GTATCCnnnnn_n'
Cuts at: 252 2025 2786 4127 252
Size: 1773 761 1341 252
Fragments arranged by size:
1773 1341 761 252
BclI T'GATC_A
Cuts at: 1647 1647
Size: 4127
BglII A'GATC_T
Cuts at: 1064 1642 3679 1064
Size: 578 2037 1512
Fragments arranged by size:
2037 1512 578
BlpI GC'TnA_GC
Cuts at: 665 1239 665
Size: 574 3553
Bme1580I G_kGCm'C
Cuts at: 1752 1845 2901 1752
Size: 93 1056 2978
Fragments arranged by size:
2978 1056 93
BmgBI CAC'GTC
Cuts at: 194 194
Size: 4127
BmrI ACTGGGnnnn_n'
Cuts at: 198 501 1339 1420 1755 3899 198
Size: 303 838 81 335 2144 426
Fragments arranged by size:
2144 838 426 335 303 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 655 1303 2182 2674 2972 494
Size: 161 648 879 492 298 1649
Fragments arranged by size:
1649 879 648 492 298 161
BsaI GGTCTCn'nnnn_
Cuts at: 3831 3831
Size: 4127
BsaAI yAC'GTr
Cuts at: 1480 1480
Size: 4127
BsaBI GATnn'nnATC
Cuts at: 6 1641 3678 6
Size: 1635 2037 455
Fragments arranged by size:
2037 1635 455
BsaHI Gr'CG_yC
Cuts at: 1558 1806 2508 4004 1558
Size: 248 702 1496 1681
Fragments arranged by size:
1681 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1316 1837 2515 2789 2936 3347 1316
Size: 521 678 274 147 411 2096
Fragments arranged by size:
2096 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 964 994 964
Size: 30 4097
BseYI C'CCAG_C
Cuts at: 1552 2310 2887 3383 1552
Size: 758 577 496 2296
Fragments arranged by size:
2296 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 520
Size: 4127
BsiEI CG_ry'CG
Cuts at: 104 1669 1715 2923 104
Size: 1565 46 1208 1308
Fragments arranged by size:
1565 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1141 1919 2109 2901 1141
Size: 778 190 792 2367
Fragments arranged by size:
2367 792 778 190
BsmI GAATG_Cn'
Cuts at: 752 752
Size: 4127
Bsp1286I G_dGCh'C
Cuts at: 429 1141 1752 1845 1919 2109 2171 2901 429
Size: 712 611 93 74 190 62 730 1655
Fragments arranged by size:
1655 730 712 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3347 3347
Size: 4127
BspHI T'CATG_A
Cuts at: 3303 3303
Size: 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1177 1391 1693 2074 1177
Size: 214 302 381 3230
Fragments arranged by size:
3230 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2419 2473 3957 4122 50
Size: 2369 54 1484 165 55
Fragments arranged by size:
2369 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2039 2039
Size: 4127
BsrFI r'CCGG_y
Cuts at: 2125 2306 2515 2125
Size: 181 209 3737
Fragments arranged by size:
3737 209 181
BssSI C'ACGA_G
Cuts at: 2398 2756 2398
Size: 358 3769
BstAPI GCAn_nnn'nTGC
Cuts at: 1504 1504
Size: 4127
BstBI TT'CG_AA
Cuts at: 2488 2488
Size: 4127
BstXI CCAn_nnnn'nTGG
Cuts at: 491 889 491
Size: 398 3729
BstYI r'GATC_y
Cuts at: 1 1064 1152 1619 1642 1977 2223 3235 3321
Size: 1063 88 467 23 335 246 1012 86
Cuts at: 3321 3333 3679 1
Size: 12 346 449
Fragments arranged by size:
1063 1012 467 449 346 335 246 88
86 23 12
BstZ17I GTA'TAC
Cuts at: 784 784
Size: 4127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2077 2258 2421 2077
Size: 181 163 3783
Fragments arranged by size:
3783 181 163
BtsI GCAGTG_nn'
Cuts at: 291 735 1370 291
Size: 444 635 3048
Fragments arranged by size:
3048 635 444
DraI TTT'AAA
Cuts at: 1126 1126
Size: 4127
DrdI GACnn_nn'nnGTC
Cuts at: 1833 2691 1833
Size: 858 3269
EaeI y'GGCC_r
Cuts at: 1712 1886 2277 2304 3649 1712
Size: 174 391 27 1345 2190
Fragments arranged by size:
2190 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1712 1712
Size: 4127
EarI CTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3917
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2335 2645 2791 3937 2335
Size: 310 146 1146 2525
Fragments arranged by size:
2525 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1139 1139
Size: 4127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1952 2384 3131 3399 1952
Size: 432 747 268 2680
Fragments arranged by size:
2680 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1266 1266
Size: 4127
EcoRI G'AATT_C
Cuts at: 611 620 1131 611
Size: 9 511 3607
Fragments arranged by size:
3607 511 9
EcoRV GAT'ATC
Cuts at: 294 294
Size: 4127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 384 429 2146 384
Size: 45 1717 2365
Fragments arranged by size:
2365 1717 45
FspI TGC'GCA
Cuts at: 1908 1908
Size: 4127
HaeII r_GCGC'y
Cuts at: 414 1561 1809 2831 3810 414
Size: 1147 248 1022 979 731
Fragments arranged by size:
1147 1022 979 731 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 576 608 1969 1999 2001 2031 576
Size: 32 1361 30 2 30 2672
Fragments arranged by size:
2672 1361 32 30 30 2
HincII GTy'rAC
Cuts at: 1166 1166
Size: 4127
HindIII A'AGCT_T
Cuts at: 303 303
Size: 4127
KasI G'GCGC_C
Cuts at: 1557 1805 1557
Size: 248 3879
KpnI G_GTAC'C
Cuts at: 725 1147 725
Size: 422 3705
MluI A'CGCG_T
Cuts at: 3560 3560
Size: 4127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2798 2982 2798
Size: 184 3943
MscI TGG'CCA
Cuts at: 1888 1888
Size: 4127
MslI CAynn'nnrTG
Cuts at: 478 2116 2243 478
Size: 1638 127 2362
Fragments arranged by size:
2362 1638 127
MspA1I CmG'CkG
Cuts at: 909 1037 1238 1552 1912 2925 3170 909
Size: 128 201 314 360 1013 245 1866
Fragments arranged by size:
1866 1013 360 314 245 201 128
NaeI GCC'GGC
Cuts at: 2308 2308
Size: 4127
NarI GG'CG_CC
Cuts at: 1558 1806 1558
Size: 248 3879
NgoMIV G'CCGG_C
Cuts at: 2306 2306
Size: 4127
NspI r_CATG'y
Cuts at: 249 993 1180 1680 2587 249
Size: 744 187 500 907 1789
Fragments arranged by size:
1789 907 744 500 187
PciI A'CATG_T
Cuts at: 245 2583 245
Size: 2338 1789
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 4095
PspXI vC'TCGA_Gb
Cuts at: 947 947
Size: 4127
PstI C_TGCA'G
Cuts at: 1174 1859 1174
Size: 685 3442
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4127
PvuII CAG'CTG
Cuts at: 1037 1552 1912 1037
Size: 515 360 3252
Fragments arranged by size:
3252 515 360
RsrII CG'GwC_CG
Cuts at: 2322 2322
Size: 4127
SacI G_AGCT'C
Cuts at: 1141 1141
Size: 4127
SalI G'TCGA_C
Cuts at: 1164 1164
Size: 4127
SapI GCTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3917
SbfI CC_TGCA'GG
Cuts at: 1174 1174
Size: 4127
ScaI AGT'ACT
Cuts at: 1105 3644 1105
Size: 2539 1588
SfcI C'TryA_G
Cuts at: 33 1170 1855 2848 3039 33
Size: 1137 685 993 191 1121
Fragments arranged by size:
1137 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1559 1807 1559
Size: 248 3879
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
SmaI CCC'GGG
Cuts at: 1149 1149
Size: 4127
SmlI C'TyrA_G
Cuts at: 509 670 947 1282 2197 2689 2951 509
Size: 161 277 335 915 492 262 1685
Fragments arranged by size:
1685 915 492 335 277 262 161
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4127
SphI G_CATG'C
Cuts at: 1180 1680 1180
Size: 500 3627
StyI C'CwwG_G
Cuts at: 1261 1615 1261
Size: 354 3773
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1710 1843 2302 2511 1710
Size: 133 459 209 3326
Fragments arranged by size:
3326 459 209 133
TatI w'GTAC_w
Cuts at: 1103 3642 1103
Size: 2539 1588
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 4095
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4127
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1622 1622
Size: 4127
XhoI C'TCGA_G
Cuts at: 947 947
Size: 4127
XmaI C'CCGG_G
Cuts at: 1147 1147
Size: 4127
XmnI GAAnn'nnTTC
Cuts at: 877 877
Size: 4127
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmtI BpmI
Bpu10I BseRI BsiWI BsmBI BsrGI BssHII BstEII Bsu36I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI