Restriction map for
pF1KB4066
(Circular) (Six-base) MAPSORT of: pF1KB4066.seq Check: 5937 from: 1 to: 4385
pF1KB4066 4385 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1228 1401 1228
Size: 173 4212
AclI AA'CG_TT
Cuts at: 152 4225 152
Size: 4073 312
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 2210 2642 3389 3657 337
Size: 1873 432 747 268 1065
Fragments arranged by size:
1873 1065 747 432 268
AflII C'TTAA_G
Cuts at: 1056 1056
Size: 4385
AflIII A'CryG_T
Cuts at: 1737 2841 3818 1737
Size: 1104 977 2304
Fragments arranged by size:
2304 1104 977
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AlwNI CAG_nnn'CTG
Cuts at: 270 1690 3257 270
Size: 1420 1567 1398
Fragments arranged by size:
1567 1420 1398
ApaLI G'TGCA_C
Cuts at: 3155 3155
Size: 4385
ApoI r'AATT_y
Cuts at: 798 841 1096 1181 1389 3738 3994 798
Size: 43 255 85 208 2349 256 1189
Fragments arranged by size:
2349 1189 256 255 208 85 43
AseI AT'TA_AT
Cuts at: 20 1271 20
Size: 1251 3134
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 1405 1405
Size: 4385
BamHI G'GATC_C
Cuts at: 765 1410 765
Size: 645 3740
BanI G'GyrC_C
Cuts at: 1228 1401 1815 2063 2098 1228
Size: 173 414 248 35 3515
Fragments arranged by size:
3515 414 248 173 35
BanII G_rGCy'C
Cuts at: 245 301 1399 2429 245
Size: 56 1098 1030 2201
Fragments arranged by size:
2201 1098 1030 56
BbeI G_GCGC'C
Cuts at: 1819 2067 1819
Size: 248 4137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4351
BciVI GTATCCnnnnn_n'
Cuts at: 2283 3044 4385 2283
Size: 761 1341 2283
Fragments arranged by size:
2283 1341 761
BclI T'GATC_A
Cuts at: 1905 1905
Size: 4385
BglI GCCn_nnn'nGGC
Cuts at: 749 749
Size: 4385
BglII A'GATC_T
Cuts at: 1008 1900 3937 1008
Size: 892 2037 1456
Fragments arranged by size:
2037 1456 892
BlpI GC'TnA_GC
Cuts at: 862 1497 862
Size: 635 3750
Bme1580I G_kGCm'C
Cuts at: 2010 2103 3159 2010
Size: 93 1056 3236
Fragments arranged by size:
3236 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1364 1597 1678 2013 4157 1364
Size: 233 81 335 2144 1592
Fragments arranged by size:
2144 1592 335 233 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 250 982 109
Size: 141 732 3512
Fragments arranged by size:
3512 732 141
Bpu10I CC'TnA_GC
Cuts at: 362 452 362
Size: 90 4295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 1561 2440 2932 3230 563
Size: 998 879 492 298 1718
Fragments arranged by size:
1718 998 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 225 471 949 4089 225
Size: 246 478 3140 521
Fragments arranged by size:
3140 521 478 246
BsaAI yAC'GTr
Cuts at: 170 1738 170
Size: 1568 2817
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 1816 2064 2766 4262 1816
Size: 248 702 1496 1939
Fragments arranged by size:
1939 1496 702 248
BsaWI w'CCGG_w
Cuts at: 408 1574 2095 2773 3047 3194 3605 408
Size: 1166 521 678 274 147 411 1188
Fragments arranged by size:
1188 1166 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 253
Size: 4385
BseYI C'CCAG_C
Cuts at: 965 1810 2568 3145 3641 965
Size: 845 758 577 496 1709
Fragments arranged by size:
1709 845 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 532
Size: 4385
BsiEI CG_ry'CG
Cuts at: 104 1927 1973 3181 104
Size: 1823 46 1208 1308
Fragments arranged by size:
1823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1399 2177 2367 3159 1399
Size: 778 190 792 2625
Fragments arranged by size:
2625 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 245 301 1399 2010 2103 2177 2367 2429 3159
Size: 56 1098 611 93 74 190 62 730
Cuts at: 3159 245
Size: 1471
Fragments arranged by size:
1471 1098 730 611 190 93 74 62
56
BspEI T'CCGG_A
Cuts at: 408 3605 408
Size: 3197 1188
BspHI T'CATG_A
Cuts at: 3561 3561
Size: 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1435 1649 1951 2332 1435
Size: 214 302 381 3488
Fragments arranged by size:
3488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2677 2731 4215 4380 50
Size: 2627 54 1484 165 55
Fragments arranged by size:
2627 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1292 2297 1292
Size: 1005 3380
BsrFI r'CCGG_y
Cuts at: 2383 2564 2773 2383
Size: 181 209 3995
Fragments arranged by size:
3995 209 181
BsrGI T'GTAC_A
Cuts at: 654 927 654
Size: 273 4112
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 858 1346 1762 858
Size: 488 416 3481
Fragments arranged by size:
3481 488 416
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstYI r'GATC_y
Cuts at: 1 765 1008 1410 1877 1900 2235 2481 3493
Size: 764 243 402 467 23 335 246 1012
Cuts at: 3493 3579 3591 3937 1
Size: 86 12 346 449
Fragments arranged by size:
1012 764 467 449 402 346 335 246
243 86 23 12
BtgI C'CryG_G
Cuts at: 304 702 304
Size: 398 3987
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2335 2516 2679 2335
Size: 181 163 4041
Fragments arranged by size:
4041 181 163
BtsI GCAGTG_nn'
Cuts at: 1357 1628 1357
Size: 271 4114
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 2091 2949 2091
Size: 858 3527
EaeI y'GGCC_r
Cuts at: 1970 2144 2535 2562 3907 1970
Size: 174 391 27 1345 2448
Fragments arranged by size:
2448 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 313 2408 2618 313
Size: 2095 210 2080
Fragments arranged by size:
2095 2080 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 213 2593 2903 3049 4195 213
Size: 2380 310 146 1146 403
Fragments arranged by size:
2380 1146 403 310 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 250 337 982 2210 2642 3389 3657 109
Size: 141 87 645 1228 432 747 268 837
Fragments arranged by size:
1228 837 747 645 432 268 141 87
EcoO109I rG'GnC_Cy
Cuts at: 1524 1524
Size: 4385
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4385
FspI TGC'GCA
Cuts at: 944 2166 944
Size: 1222 3163
HaeII r_GCGC'y
Cuts at: 1819 2067 3089 4068 1819
Size: 248 1022 979 2136
Fragments arranged by size:
2136 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 474 506 2227 2257 2259 2289 474
Size: 32 1721 30 2 30 2570
Fragments arranged by size:
2570 1721 32 30 30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
KasI G'GCGC_C
Cuts at: 1815 2063 1815
Size: 248 4137
KpnI G_GTAC'C
Cuts at: 1232 1405 1232
Size: 173 4212
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1143 3056 3240 1143
Size: 1913 184 2288
Fragments arranged by size:
2288 1913 184
MscI TGG'CCA
Cuts at: 2146 2146
Size: 4385
MslI CAynn'nnrTG
Cuts at: 554 2374 2501 554
Size: 1820 127 2438
Fragments arranged by size:
2438 1820 127
MspA1I CmG'CkG
Cuts at: 599 869 969 1496 1810 2170 3183 3428 599
Size: 270 100 527 314 360 1013 245 1556
Fragments arranged by size:
1556 1013 527 360 314 270 245 100
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 1816 2064 1816
Size: 248 4137
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NspI r_CATG'y
Cuts at: 928 949 1438 1938 2845 928
Size: 21 489 500 907 2468
Fragments arranged by size:
2468 907 500 489 21
PciI A'CATG_T
Cuts at: 2841 2841
Size: 4385
PflMI CCAn_nnn'nTGG
Cuts at: 627 1309 627
Size: 682 3703
PfoI T'CCnGG_A
Cuts at: 125 125
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PmlI CAC'GTG
Cuts at: 170 170
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PstI C_TGCA'G
Cuts at: 854 1432 2117 854
Size: 578 685 3122
Fragments arranged by size:
3122 685 578
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 869 969 1810 2170 869
Size: 100 841 360 3084
Fragments arranged by size:
3084 841 360 100
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 313 2408 2618 313
Size: 2095 210 2080
Fragments arranged by size:
2095 2080 210
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 850 984 1264 1428 2113 3106 3297 33
Size: 817 134 280 164 685 993 191 1121
Fragments arranged by size:
1121 993 817 685 280 191 164 134
SfoI GGC'GCC
Cuts at: 1817 2065 1817
Size: 248 4137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 578 1056 1540 2455 2947 3209 578
Size: 478 484 915 492 262 1754
Fragments arranged by size:
1754 915 492 484 478 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 1438 1938 1438
Size: 500 3885
StuI AGG'CCT
Cuts at: 360 752 360
Size: 392 3993
StyI C'CwwG_G
Cuts at: 1519 1873 1519
Size: 354 4031
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 812 979 1968 2101 2560 2769 812
Size: 167 989 133 459 209 2428
Fragments arranged by size:
2428 989 459 209 167 133
TatI w'GTAC_w
Cuts at: 654 927 3900 654
Size: 273 2973 1139
Fragments arranged by size:
2973 1139 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 4353
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1050 1416 1050
Size: 366 4019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 392 1880 392
Size: 1488 2897
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
XmnI GAAnn'nnTTC
Cuts at: 432 1253 432
Size: 821 3564
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI