Restriction map for
pF1KB4062
(Circular) (Six-base) MAPSORT of: pF1KB4062.seq Check: 8220 from: 1 to: 3638
pF1KB4062 3638 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 676 676
Size: 3638
Acc65I G'GTAC_C
Cuts at: 654 654
Size: 3638
AclI AA'CG_TT
Cuts at: 3478 3478
Size: 3638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1895 2642 2910 1463
Size: 432 747 268 2191
Fragments arranged by size:
2191 747 432 268
AflIII A'CryG_T
Cuts at: 990 2094 3071 990
Size: 1104 977 1557
Fragments arranged by size:
1557 1104 977
AgeI A'CCGG_T
Cuts at: 2026 2026
Size: 3638
AhdI GACnn_n'nnGTC
Cuts at: 353 353
Size: 3638
AlwNI CAG_nnn'CTG
Cuts at: 414 483 551 943 2510 414
Size: 69 68 392 1567 1542
Fragments arranged by size:
1567 1542 392 69 68
ApaLI G'TGCA_C
Cuts at: 2408 2408
Size: 3638
ApoI r'AATT_y
Cuts at: 642 2991 3247 642
Size: 2349 256 1033
Fragments arranged by size:
2349 1033 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
AvaI C'yCGr_G
Cuts at: 658 658
Size: 3638
BamHI G'GATC_C
Cuts at: 152 663 152
Size: 511 3127
BanI G'GyrC_C
Cuts at: 654 1068 1316 1351 654
Size: 414 248 35 2941
Fragments arranged by size:
2941 414 248 35
BanII G_rGCy'C
Cuts at: 605 652 1682 605
Size: 47 1030 2561
Fragments arranged by size:
2561 1030 47
BbeI G_GCGC'C
Cuts at: 1072 1320 1072
Size: 248 3390
BciVI GTATCCnnnnn_n'
Cuts at: 1536 2297 3638 1536
Size: 761 1341 1536
Fragments arranged by size:
1536 1341 761
BclI T'GATC_A
Cuts at: 1158 1158
Size: 3638
BglI GCCn_nnn'nGGC
Cuts at: 327 327
Size: 3638
BglII A'GATC_T
Cuts at: 1153 3190 1153
Size: 2037 1601
BlpI GC'TnA_GC
Cuts at: 750 750
Size: 3638
Bme1580I G_kGCm'C
Cuts at: 1263 1356 2412 1263
Size: 93 1056 2489
Fragments arranged by size:
2489 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 353 532 850 931 1266 3410 353
Size: 179 318 81 335 2144 581
Fragments arranged by size:
2144 581 335 318 179 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1693 2185 2483 814
Size: 879 492 298 1969
Fragments arranged by size:
1969 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 361 3342 361
Size: 2981 657
BsaAI yAC'GTr
Cuts at: 991 991
Size: 3638
BsaBI GATnn'nnATC
Cuts at: 6 1152 3189 6
Size: 1146 2037 455
Fragments arranged by size:
2037 1146 455
BsaHI Gr'CG_yC
Cuts at: 449 1069 1317 2019 3515 449
Size: 620 248 702 1496 572
Fragments arranged by size:
1496 702 620 572 248
BsaWI w'CCGG_w
Cuts at: 827 1348 2026 2300 2447 2858 827
Size: 521 678 274 147 411 1607
Fragments arranged by size:
1607 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 210 1063 1821 2398 2894 210
Size: 853 758 577 496 954
Fragments arranged by size:
954 853 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 580 400
Size: 180 3458
BsiEI CG_ry'CG
Cuts at: 104 179 322 384 390 1180 1226 2434 104
Size: 75 143 62 6 790 46 1208 1308
Fragments arranged by size:
1308 1208 790 143 75 62 46 6
BsiHKAI G_wGCw'C
Cuts at: 652 1430 1620 2412 652
Size: 778 190 792 1878
Fragments arranged by size:
1878 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 250 250
Size: 3638
Bsp1286I G_dGCh'C
Cuts at: 605 652 1263 1356 1430 1620 1682 2412 605
Size: 47 611 93 74 190 62 730 1831
Fragments arranged by size:
1831 730 611 190 93 74 62 47
BspEI T'CCGG_A
Cuts at: 2858 2858
Size: 3638
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 491 688 902 1204 1585 491
Size: 197 214 302 381 2544
Fragments arranged by size:
2544 381 302 214 197
BsrBI CCG'CTC
Cuts at: 50 324 1930 1984 3468 3633 50
Size: 274 1606 54 1484 165 55
Fragments arranged by size:
1606 1484 274 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 1550
Size: 3638
BsrFI r'CCGG_y
Cuts at: 178 1636 1817 2026 178
Size: 1458 181 209 1790
Fragments arranged by size:
1790 1458 209 181
BsrGI T'GTAC_A
Cuts at: 197 197
Size: 3638
BssSI C'ACGA_G
Cuts at: 505 1909 2267 505
Size: 1404 358 1876
Fragments arranged by size:
1876 1404 358
BstAPI GCAn_nnn'nTGC
Cuts at: 483 1015 483
Size: 532 3106
BstBI TT'CG_AA
Cuts at: 1999 1999
Size: 3638
BstEII G'GTnAC_C
Cuts at: 222 222
Size: 3638
BstYI r'GATC_y
Cuts at: 1 152 314 509 663 1130 1153 1488 1734
Size: 151 162 195 154 467 23 335 246
Cuts at: 1734 2746 2832 2844 3190 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 195 162
154 151 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 579 579
Size: 3638
BtgI C'CryG_G
Cuts at: 107 107
Size: 3638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1588 1769 1932 1588
Size: 181 163 3294
Fragments arranged by size:
3294 181 163
BtsI GCAGTG_nn'
Cuts at: 881 881
Size: 3638
ClaI AT'CG_AT
Cuts at: 395 395
Size: 3638
DraI TTT'AAA
Cuts at: 637 637
Size: 3638
DrdI GACnn_nn'nnGTC
Cuts at: 1344 2202 1344
Size: 858 2780
EaeI y'GGCC_r
Cuts at: 176 303 319 1223 1397 1788 1815 3160 176
Size: 127 16 904 174 391 27 1345 654
Fragments arranged by size:
1345 904 654 391 174 127 27 16
EagI C'GGCC_G
Cuts at: 176 319 1223 176
Size: 143 904 2591
Fragments arranged by size:
2591 904 143
EarI CTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1846 2156 2302 3448 1846
Size: 310 146 1146 2036
Fragments arranged by size:
2036 1146 310 146
EcoICRI GAG'CTC
Cuts at: 650 650
Size: 3638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1895 2642 2910 1463
Size: 432 747 268 2191
Fragments arranged by size:
2191 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 347 480 777 347
Size: 133 297 3208
Fragments arranged by size:
3208 297 133
EcoRI G'AATT_C
Cuts at: 642 642
Size: 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1657 1657
Size: 3638
FspI TGC'GCA
Cuts at: 1419 1419
Size: 3638
HaeII r_GCGC'y
Cuts at: 1072 1320 2342 3321 1072
Size: 248 1022 979 1389
Fragments arranged by size:
1389 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 339 371 1480 1510 1512 1542 339
Size: 32 1109 30 2 30 2435
Fragments arranged by size:
2435 1109 32 30 30 2
HincII GTy'rAC
Cuts at: 614 677 614
Size: 63 3575
HpaI GTT'AAC
Cuts at: 614 614
Size: 3638
KasI G'GCGC_C
Cuts at: 1068 1316 1068
Size: 248 3390
KpnI G_GTAC'C
Cuts at: 658 658
Size: 3638
MluI A'CGCG_T
Cuts at: 3071 3071
Size: 3638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2493 2309
Size: 184 3454
MscI TGG'CCA
Cuts at: 1399 1399
Size: 3638
MslI CAynn'nnrTG
Cuts at: 1627 1754 1627
Size: 127 3511
MspA1I CmG'CkG
Cuts at: 749 1063 1423 2436 2681 749
Size: 314 360 1013 245 1706
Fragments arranged by size:
1706 1013 360 314 245
NaeI GCC'GGC
Cuts at: 180 1819 180
Size: 1639 1999
NarI GG'CG_CC
Cuts at: 1069 1317 1069
Size: 248 3390
NcoI C'CATG_G
Cuts at: 107 107
Size: 3638
NgoMIV G'CCGG_C
Cuts at: 178 1817 178
Size: 1639 1999
NspI r_CATG'y
Cuts at: 691 1191 2098 691
Size: 500 907 2231
Fragments arranged by size:
2231 907 500
PciI A'CATG_T
Cuts at: 2094 2094
Size: 3638
PfoI T'CCnGG_A
Cuts at: 512 512
Size: 3638
PmeI GTTT'AAAC
Cuts at: 637 637
Size: 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 3606
PpuMI rG'GwC_Cy
Cuts at: 347 480 347
Size: 133 3505
PstI C_TGCA'G
Cuts at: 685 1370 685
Size: 685 2953
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3638
PvuII CAG'CTG
Cuts at: 1063 1423 1063
Size: 360 3278
RsrII CG'GwC_CG
Cuts at: 1833 1833
Size: 3638
SacI G_AGCT'C
Cuts at: 652 652
Size: 3638
SalI G'TCGA_C
Cuts at: 675 675
Size: 3638
SanDI GG'GwC_CC
Cuts at: 347 347
Size: 3638
SapI GCTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
SbfI CC_TGCA'GG
Cuts at: 685 685
Size: 3638
ScaI AGT'ACT
Cuts at: 3155 3155
Size: 3638
SfcI C'TryA_G
Cuts at: 33 681 1366 2359 2550 33
Size: 648 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 191
SfoI GGC'GCC
Cuts at: 1070 1318 1070
Size: 248 3390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
SmaI CCC'GGG
Cuts at: 660 660
Size: 3638
SmlI C'TyrA_G
Cuts at: 793 1708 2200 2462 793
Size: 915 492 262 1969
Fragments arranged by size:
1969 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3638
SphI G_CATG'C
Cuts at: 691 1191 691
Size: 500 3138
StyI C'CwwG_G
Cuts at: 107 351 772 1126 107
Size: 244 421 354 2619
Fragments arranged by size:
2619 421 354 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1221 1354 1813 2022 1221
Size: 133 459 209 2837
Fragments arranged by size:
2837 459 209 133
TatI w'GTAC_w
Cuts at: 197 3153 197
Size: 2956 682
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3606
Tth111I GACn'n_nGTC
Cuts at: 1435 1435
Size: 3638
XbaI T'CTAG_A
Cuts at: 669 669
Size: 3638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 237 1133 237
Size: 896 2742
XmaI C'CCGG_G
Cuts at: 658 658
Size: 3638
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BssHII
BstXI BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI