Restriction map for
pF1KB4057
(Circular) (Six-base) MAPSORT of: pF1KB4057.seq Check: 4918 from: 1 to: 5174
pF1KB4057 5174 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 1580 1580
Size: 5174
AccI GT'mk_AC
Cuts at: 269 761 1313 2212 269
Size: 492 552 899 3231
Fragments arranged by size:
3231 899 552 492
Acc65I G'GTAC_C
Cuts at: 2190 2190
Size: 5174
AclI AA'CG_TT
Cuts at: 5014 5014
Size: 5174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 739 1399 2146 2999 3431 3784 4446 267
Size: 472 660 747 853 432 353 662 995
Fragments arranged by size:
995 853 747 662 660 472 432 353
AflIII A'CryG_T
Cuts at: 2526 4326 4607 2526
Size: 1800 281 3093
Fragments arranged by size:
3093 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1335 1335
Size: 5174
AleI CACnn'nnGTG
Cuts at: 382 382
Size: 5174
AlwNI CAG_nnn'CTG
Cuts at: 1176 2479 3917 1176
Size: 1303 1438 2433
Fragments arranged by size:
2433 1438 1303
ApaI G_GGCC'C
Cuts at: 1673 1803 1673
Size: 130 5044
ApaLI G'TGCA_C
Cuts at: 1342 4012 1342
Size: 2670 2504
ApoI r'AATT_y
Cuts at: 648 2178 4527 4783 648
Size: 1530 2349 256 1039
Fragments arranged by size:
2349 1530 1039 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5174
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5174
AvaI C'yCGr_G
Cuts at: 1672 2194 1672
Size: 522 4652
BamHI G'GATC_C
Cuts at: 487 671 1319 1535 1883 2199 487
Size: 184 648 216 348 316 3462
Fragments arranged by size:
3462 648 348 316 216 184
BanI G'GyrC_C
Cuts at: 533 974 1681 2190 2604 2852 2887 533
Size: 441 707 509 414 248 35 2820
Fragments arranged by size:
2820 707 509 441 414 248 35
BanII G_rGCy'C
Cuts at: 619 1673 1803 1898 2188 3218 619
Size: 1054 130 95 290 1030 2575
Fragments arranged by size:
2575 1054 1030 290 130 95
BbeI G_GCGC'C
Cuts at: 2608 2856 2608
Size: 248 4926
BbsI GAAGACnn'nnnn_
Cuts at: 936 2014 936
Size: 1078 4096
BbvCI CC'TCA_GC
Cuts at: 457 970 1607 1922 457
Size: 513 637 315 3709
Fragments arranged by size:
3709 637 513 315
BciVI GTATCCnnnnn_n'
Cuts at: 3072 4128 5174 3072
Size: 1056 1046 3072
Fragments arranged by size:
3072 1056 1046
BclI T'GATC_A
Cuts at: 2694 2694
Size: 5174
BglI GCCn_nnn'nGGC
Cuts at: 225 955 225
Size: 730 4444
BglII A'GATC_T
Cuts at: 2689 4726 2689
Size: 2037 3137
BlpI GC'TnA_GC
Cuts at: 2286 2286
Size: 5174
Bme1580I G_kGCm'C
Cuts at: 538 1346 1641 1673 1803 2799 2892 4016 538
Size: 808 295 32 130 996 93 1124 1696
Fragments arranged by size:
1696 1124 996 808 295 130 93 32
BmgBI CAC'GTC
Cuts at: 1308 1308
Size: 5174
BmrI ACTGGGnnnn_n'
Cuts at: 1219 2386 2467 2802 4946 1219
Size: 1167 81 335 2144 1447
Fragments arranged by size:
2144 1447 1167 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1470 1734 1751 3541 1470
Size: 264 17 1790 3103
Fragments arranged by size:
3103 1790 264 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 169 484 1729 1879 163
Size: 6 315 1245 150 3458
Fragments arranged by size:
3458 1245 315 150 6
Bpu10I CC'TnA_GC
Cuts at: 457 970 1258 1607 1922 457
Size: 513 288 349 315 3709
Fragments arranged by size:
3709 513 349 315 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2350 3229 3943 4241 2350
Size: 879 714 298 3283
Fragments arranged by size:
3283 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 145 158 4878 145
Size: 13 4720 441
Fragments arranged by size:
4720 441 13
BsaAI yAC'GTr
Cuts at: 765 2527 765
Size: 1762 3412
BsaBI GATnn'nnATC
Cuts at: 6 1882 2688 4725 6
Size: 1876 806 2037 455
Fragments arranged by size:
2037 1876 806 455
BsaHI Gr'CG_yC
Cuts at: 851 1577 2605 2853 3555 5051 851
Size: 726 1028 248 702 1496 974
Fragments arranged by size:
1496 1028 974 726 702 248
BsaWI w'CCGG_w
Cuts at: 1880 2144 2363 2884 3562 3973 4120 4394 1880
Size: 264 219 521 678 411 147 274 2660
Fragments arranged by size:
2660 678 521 411 274 264 219 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 373 583 1474 1528 1624 1795 2014 130
Size: 243 210 891 54 96 171 219 3290
Fragments arranged by size:
3290 891 243 219 210 171 96 54
BseYI C'CCAG_C
Cuts at: 300 501 537 593 819 827 938 1115 2599
Size: 201 36 56 226 8 111 177 1484
Cuts at: 2599 3357 4022 4430 300
Size: 758 665 408 1044
Fragments arranged by size:
1484 1044 758 665 408 226 201 177
111 56 36 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 384 945 1950 220
Size: 164 561 1005 3444
Fragments arranged by size:
3444 1005 561 164
BsiEI CG_ry'CG
Cuts at: 104 179 732 2716 2762 3992 104
Size: 75 553 1984 46 1230 1286
Fragments arranged by size:
1984 1286 1230 553 75 46
BsiHKAI G_wGCw'C
Cuts at: 1264 1346 2188 2966 3156 4016 1264
Size: 82 842 778 190 860 2422
Fragments arranged by size:
2422 860 842 778 190 82
BsmBI CGTCTCn'nnnn_
Cuts at: 1400 1400
Size: 5174
Bsp1286I G_dGCh'C
Cuts at: 538 619 1264 1346 1641 1673 1803 1898 2188
Size: 81 645 82 295 32 130 95 290
Cuts at: 2188 2799 2892 2966 3156 3218 4016 538
Size: 611 93 74 190 62 798 1696
Fragments arranged by size:
1696 798 645 611 295 290 190 130
95 93 82 81 74 62 32
BspEI T'CCGG_A
Cuts at: 1880 2144 1880
Size: 264 4910
BspHI T'CATG_A
Cuts at: 3606 3606
Size: 5174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1154 1700 1841 2075 2224 2438 2740 3121 1154
Size: 546 141 234 149 214 302 381 3207
Fragments arranged by size:
3207 546 381 302 234 214 149 141
BsrBI CCG'CTC
Cuts at: 50 573 1413 1944 2076 3466 3520 5004 5169
Size: 523 840 531 132 1390 54 1484 165
Cuts at: 5169 50
Size: 55
Fragments arranged by size:
1484 1390 840 531 523 165 132 55
54
BsrDI GCAATG_nn'
Cuts at: 1065 3086 1065
Size: 2021 3153
BsrFI r'CCGG_y
Cuts at: 295 727 1987 3172 3353 295
Size: 432 1260 1185 181 2116
Fragments arranged by size:
2116 1260 1185 432 181
BssSI C'ACGA_G
Cuts at: 1807 3445 4153 1807
Size: 1638 708 2828
Fragments arranged by size:
2828 1638 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2551 2551
Size: 5174
BstBI TT'CG_AA
Cuts at: 3535 3535
Size: 5174
BstXI CCAn_nnnn'nTGG
Cuts at: 384 384
Size: 5174
BstYI r'GATC_y
Cuts at: 1 487 671 1073 1319 1535 1661 1877 1883
Size: 486 184 402 246 216 126 216 6
Cuts at: 1883 2018 2199 2666 2689 3024 3270 3576 3588
Size: 135 181 467 23 335 246 306 12
Cuts at: 3588 3674 4726 1
Size: 86 1052 449
Fragments arranged by size:
1052 486 467 449 402 335 306 246
246 216 216 184 181 135 126 86
23 12 6
BtgI C'CryG_G
Cuts at: 107 380 906 953 2030 107
Size: 273 526 47 1077 3251
Fragments arranged by size:
3251 1077 526 273 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3124 3305 3468 3124
Size: 181 163 4830
Fragments arranged by size:
4830 181 163
BtsI GCAGTG_nn'
Cuts at: 1110 1572 2417 1110
Size: 462 845 3867
Fragments arranged by size:
3867 845 462
DraI TTT'AAA
Cuts at: 2173 2173
Size: 5174
DrdI GACnn_nn'nnGTC
Cuts at: 1576 2880 4224 1576
Size: 1304 1344 2526
Fragments arranged by size:
2526 1344 1304
EaeI y'GGCC_r
Cuts at: 110 159 176 428 620 729 930 1502 1526
Size: 49 17 252 192 109 201 572 24
Cuts at: 1526 2759 2933 3324 3351 4696 110
Size: 1233 174 391 27 1345 588
Fragments arranged by size:
1345 1233 588 572 391 252 201 192
174 109 49 27 24 17
EagI C'GGCC_G
Cuts at: 176 729 2759 176
Size: 553 2030 2591
Fragments arranged by size:
2591 2030 553
EarI CTCTTCn'nnn_
Cuts at: 526 560 1192 1720 3197 3407 526
Size: 34 632 528 1477 210 2293
Fragments arranged by size:
2293 1477 632 528 210 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3382 4124 4270 4984 3382
Size: 742 146 714 3572
Fragments arranged by size:
3572 742 714 146
EcoICRI GAG'CTC
Cuts at: 2186 2186
Size: 5174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 169 267 484 739 1399 1729 1879 2146
Size: 6 98 217 255 660 330 150 267
Cuts at: 2146 2999 3431 3784 4446 163
Size: 853 432 353 662 891
Fragments arranged by size:
891 853 662 660 432 353 330 267
255 217 150 98 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1476 1789 1996 1476
Size: 313 207 4654
Fragments arranged by size:
4654 313 207
EcoO109I rG'GnC_Cy
Cuts at: 1173 1451 1669 1800 1848 2313 1173
Size: 278 218 131 48 465 4034
Fragments arranged by size:
4034 465 278 218 131 48
EcoRI G'AATT_C
Cuts at: 2178 2178
Size: 5174
EcoRV GAT'ATC
Cuts at: 1422 1422
Size: 5174
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3193 3193
Size: 5174
FseI GG_CCGG'CC
Cuts at: 1991 1991
Size: 5174
FspI TGC'GCA
Cuts at: 2955 2955
Size: 5174
HaeII r_GCGC'y
Cuts at: 897 2608 2856 4086 4857 897
Size: 1711 248 1230 771 1214
Fragments arranged by size:
1711 1230 1214 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 123 155 1321 1353 3016 3046 3048 3078 123
Size: 32 1166 32 1663 30 2 30 2219
Fragments arranged by size:
2219 1663 1166 32 32 30 30 2
HincII GTy'rAC
Cuts at: 705 792 2213 705
Size: 87 1421 3666
Fragments arranged by size:
3666 1421 87
KasI G'GCGC_C
Cuts at: 2604 2852 2604
Size: 248 4926
KpnI G_GTAC'C
Cuts at: 2194 2194
Size: 5174
MluI A'CGCG_T
Cuts at: 4607 4607
Size: 5174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1805 3933 4117 1805
Size: 2128 184 2862
Fragments arranged by size:
2862 2128 184
MscI TGG'CCA
Cuts at: 932 1528 2935 932
Size: 596 1407 3171
Fragments arranged by size:
3171 1407 596
MslI CAynn'nnrTG
Cuts at: 382 1558 1792 3163 3290 382
Size: 1176 234 1371 127 2266
Fragments arranged by size:
2266 1371 1176 234 127
MspA1I CmG'CkG
Cuts at: 541 1606 2285 2599 2959 3743 3988 541
Size: 1065 679 314 360 784 245 1727
Fragments arranged by size:
1727 1065 784 679 360 314 245
NaeI GCC'GGC
Cuts at: 729 1989 3355 729
Size: 1260 1366 2548
Fragments arranged by size:
2548 1366 1260
NarI GG'CG_CC
Cuts at: 2605 2853 2605
Size: 248 4926
NcoI C'CATG_G
Cuts at: 107 107
Size: 5174
NgoMIV G'CCGG_C
Cuts at: 727 1987 3353 727
Size: 1260 1366 2548
Fragments arranged by size:
2548 1366 1260
NspI r_CATG'y
Cuts at: 2227 2727 4330 2227
Size: 500 1603 3071
Fragments arranged by size:
3071 1603 500
PciI A'CATG_T
Cuts at: 4326 4326
Size: 5174
PflMI CCAn_nnn'nTGG
Cuts at: 866 2097 866
Size: 1231 3943
PfoI T'CCnGG_A
Cuts at: 865 865
Size: 5174
PmeI GTTT'AAAC
Cuts at: 2173 2173
Size: 5174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 5142
PpuMI rG'GwC_Cy
Cuts at: 1451 1848 1451
Size: 397 4777
PspOMI G'GGCC_C
Cuts at: 1669 1799 1669
Size: 130 5044
PstI C_TGCA'G
Cuts at: 416 1151 1937 2221 2906 416
Size: 735 786 284 685 2684
Fragments arranged by size:
2684 786 735 685 284
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5174
PvuII CAG'CTG
Cuts at: 2599 2959 2599
Size: 360 4814
RsrII CG'GwC_CG
Cuts at: 3369 3369
Size: 5174
SacI G_AGCT'C
Cuts at: 2188 2188
Size: 5174
SalI G'TCGA_C
Cuts at: 2211 2211
Size: 5174
SapI GCTCTTCn'nnn_
Cuts at: 526 1192 1720 3197 3407 526
Size: 666 528 1477 210 2293
Fragments arranged by size:
2293 1477 666 528 210
SbfI CC_TGCA'GG
Cuts at: 2221 2221
Size: 5174
ScaI AGT'ACT
Cuts at: 1051 4691 1051
Size: 3640 1534
SfcI C'TryA_G
Cuts at: 33 412 1147 1933 2217 2902 3870 4061 33
Size: 379 735 786 284 685 968 191 1146
Fragments arranged by size:
1146 968 786 735 685 379 284 191
SfoI GGC'GCC
Cuts at: 2606 2854 2606
Size: 248 4926
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5174
SmaI CCC'GGG
Cuts at: 2196 2196
Size: 5174
SmlI C'TyrA_G
Cuts at: 2329 3244 3958 4220 2329
Size: 915 714 262 3283
Fragments arranged by size:
3283 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5174
SphI G_CATG'C
Cuts at: 2227 2727 2227
Size: 500 4674
StuI AGG'CCT
Cuts at: 469 1471 469
Size: 1002 4172
StyI C'CwwG_G
Cuts at: 107 326 643 1887 2308 2662 107
Size: 219 317 1244 421 354 2619
Fragments arranged by size:
2619 1244 421 354 317 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 607 1107 1441 2757 2890 3349 3558 607
Size: 500 334 1316 133 459 209 2223
Fragments arranged by size:
2223 1316 500 459 334 209 133
TatI w'GTAC_w
Cuts at: 1049 4689 1049
Size: 3640 1534
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4691 4723 4691
Size: 32 5142
Tth111I GACn'n_nGTC
Cuts at: 850 2971 850
Size: 2121 3053
XbaI T'CTAG_A
Cuts at: 696 2205 696
Size: 1509 3665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 498 1330 2669 498
Size: 832 1339 3003
Fragments arranged by size:
3003 1339 832
XmaI C'CCGG_G
Cuts at: 2194 2194
Size: 5174
XmnI GAAnn'nnTTC
Cuts at: 2140 2140
Size: 5174
ZraI GAC'GTC
Cuts at: 1578 1578
Size: 5174
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI