Restriction map for
pF1KB4051
(Circular) (Six-base) MAPSORT of: pF1KB4051.seq Check: 2371 from: 1 to: 4136
pF1KB4051 4136 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1174 1174
Size: 4136
Acc65I G'GTAC_C
Cuts at: 1152 1152
Size: 4136
AclI AA'CG_TT
Cuts at: 3976 3976
Size: 4136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 809 1961 2393 3140 3408 529
Size: 280 1152 432 747 268 1257
Fragments arranged by size:
1257 1152 747 432 280 268
AflII C'TTAA_G
Cuts at: 601 601
Size: 4136
AflIII A'CryG_T
Cuts at: 1488 2592 3569 1488
Size: 1104 977 2055
Fragments arranged by size:
2055 1104 977
AgeI A'CCGG_T
Cuts at: 2524 2524
Size: 4136
AlwNI CAG_nnn'CTG
Cuts at: 706 1441 3008 706
Size: 735 1567 1834
Fragments arranged by size:
1834 1567 735
ApaLI G'TGCA_C
Cuts at: 2906 2906
Size: 4136
ApoI r'AATT_y
Cuts at: 221 1140 3489 3745 221
Size: 919 2349 256 612
Fragments arranged by size:
2349 919 612 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4136
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
AvaI C'yCGr_G
Cuts at: 1156 1156
Size: 4136
BamHI G'GATC_C
Cuts at: 1161 1161
Size: 4136
BanI G'GyrC_C
Cuts at: 1152 1566 1814 1849 1152
Size: 414 248 35 3439
Fragments arranged by size:
3439 414 248 35
BanII G_rGCy'C
Cuts at: 1150 2180 1150
Size: 1030 3106
BbeI G_GCGC'C
Cuts at: 1570 1818 1570
Size: 248 3888
BciVI GTATCCnnnnn_n'
Cuts at: 633 2034 2795 4136 633
Size: 1401 761 1341 633
Fragments arranged by size:
1401 1341 761 633
BclI T'GATC_A
Cuts at: 1656 1656
Size: 4136
BfrBI ATG'CAT
Cuts at: 326 326
Size: 4136
BglII A'GATC_T
Cuts at: 597 1651 3688 597
Size: 1054 2037 1045
Fragments arranged by size:
2037 1054 1045
BlpI GC'TnA_GC
Cuts at: 550 1248 550
Size: 698 3438
Bme1580I G_kGCm'C
Cuts at: 276 561 1761 1854 2910 276
Size: 285 1200 93 1056 1502
Fragments arranged by size:
1502 1200 1056 285 93
BmrI ACTGGGnnnn_n'
Cuts at: 1348 1429 1764 3908 1348
Size: 81 335 2144 1576
Fragments arranged by size:
2144 1576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 4136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 2191 2683 2981 1312
Size: 879 492 298 2467
Fragments arranged by size:
2467 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3840 3840
Size: 4136
BsaAI yAC'GTr
Cuts at: 1489 1489
Size: 4136
BsaBI GATnn'nnATC
Cuts at: 6 844 1650 3687 6
Size: 838 806 2037 455
Fragments arranged by size:
2037 838 806 455
BsaHI Gr'CG_yC
Cuts at: 1567 1815 2517 4013 1567
Size: 248 702 1496 1690
Fragments arranged by size:
1690 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1325 1846 2524 2798 2945 3356 1325
Size: 521 678 274 147 411 2105
Fragments arranged by size:
2105 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 494 494
Size: 4136
BseYI C'CCAG_C
Cuts at: 1121 1561 2319 2896 3392 1121
Size: 440 758 577 496 1865
Fragments arranged by size:
1865 758 577 496 440
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 185
Size: 4136
BsiEI CG_ry'CG
Cuts at: 104 1678 1724 2932 104
Size: 1574 46 1208 1308
Fragments arranged by size:
1574 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1150 1928 2118 2910 1150
Size: 778 190 792 2376
Fragments arranged by size:
2376 792 778 190
BsmI GAATG_Cn'
Cuts at: 328 328
Size: 4136
Bsp1286I G_dGCh'C
Cuts at: 276 561 1150 1761 1854 1928 2118 2180 2910
Size: 285 589 611 93 74 190 62 730
Cuts at: 2910 276
Size: 1502
Fragments arranged by size:
1502 730 611 589 285 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3356 3356
Size: 4136
BspHI T'CATG_A
Cuts at: 3312 3312
Size: 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1186 1400 1702 2083 1186
Size: 214 302 381 3239
Fragments arranged by size:
3239 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2428 2482 3966 4131 50
Size: 2378 54 1484 165 55
Fragments arranged by size:
2378 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 860 2048 860
Size: 1188 2948
BsrFI r'CCGG_y
Cuts at: 2134 2315 2524 2134
Size: 181 209 3746
Fragments arranged by size:
3746 209 181
BssSI C'ACGA_G
Cuts at: 2407 2765 2407
Size: 358 3778
BstAPI GCAn_nnn'nTGC
Cuts at: 1513 1513
Size: 4136
BstBI TT'CG_AA
Cuts at: 2497 2497
Size: 4136
BstXI CCAn_nnnn'nTGG
Cuts at: 651 651
Size: 4136
BstYI r'GATC_y
Cuts at: 1 597 1161 1628 1651 1986 2232 3244 3330
Size: 596 564 467 23 335 246 1012 86
Cuts at: 3330 3342 3688 1
Size: 12 346 449
Fragments arranged by size:
1012 596 564 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1055 1055
Size: 4136
BtgI C'CryG_G
Cuts at: 107 363 107
Size: 256 3880
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2086 2267 2430 2086
Size: 181 163 3792
Fragments arranged by size:
3792 181 163
BtsI GCAGTG_nn'
Cuts at: 218 561 1379 218
Size: 343 818 2975
Fragments arranged by size:
2975 818 343
DraI TTT'AAA
Cuts at: 1110 1135 1110
Size: 25 4111
DrdI GACnn_nn'nnGTC
Cuts at: 1842 2700 1842
Size: 858 3278
EaeI y'GGCC_r
Cuts at: 1721 1895 2286 2313 3658 1721
Size: 174 391 27 1345 2199
Fragments arranged by size:
2199 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1721 1721
Size: 4136
EarI CTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2344 2654 2800 3946 2344
Size: 310 146 1146 2534
Fragments arranged by size:
2534 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1148 1148
Size: 4136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 809 1961 2393 3140 3408 529
Size: 280 1152 432 747 268 1257
Fragments arranged by size:
1257 1152 747 432 280 268
EcoNI CCTnn'n_nnAGG
Cuts at: 334 334
Size: 4136
EcoO109I rG'GnC_Cy
Cuts at: 1275 1275
Size: 4136
EcoRI G'AATT_C
Cuts at: 1140 1140
Size: 4136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 918 2155 918
Size: 1237 2899
FspI TGC'GCA
Cuts at: 1917 1917
Size: 4136
HaeII r_GCGC'y
Cuts at: 443 1570 1818 2840 3819 443
Size: 1127 248 1022 979 760
Fragments arranged by size:
1127 1022 979 760 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 282 314 1978 2008 2010 2040 282
Size: 32 1664 30 2 30 2378
Fragments arranged by size:
2378 1664 32 30 30 2
HincII GTy'rAC
Cuts at: 1175 1175
Size: 4136
KasI G'GCGC_C
Cuts at: 1566 1814 1566
Size: 248 3888
KpnI G_GTAC'C
Cuts at: 1156 1156
Size: 4136
MluI A'CGCG_T
Cuts at: 3569 3569
Size: 4136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 617 2807 2991 617
Size: 2190 184 1762
Fragments arranged by size:
2190 1762 184
MscI TGG'CCA
Cuts at: 1897 1897
Size: 4136
MslI CAynn'nnrTG
Cuts at: 302 988 2125 2252 302
Size: 686 1137 127 2186
Fragments arranged by size:
2186 1137 686 127
MspA1I CmG'CkG
Cuts at: 549 1121 1247 1561 1921 2934 3179 549
Size: 572 126 314 360 1013 245 1506
Fragments arranged by size:
1506 1013 572 360 314 245 126
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 4136
NarI GG'CG_CC
Cuts at: 1567 1815 1567
Size: 248 3888
NcoI C'CATG_G
Cuts at: 107 107
Size: 4136
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 4136
NsiI A_TGCA'T
Cuts at: 328 328
Size: 4136
NspI r_CATG'y
Cuts at: 764 1189 1689 2596 764
Size: 425 500 907 2304
Fragments arranged by size:
2304 907 500 425
PciI A'CATG_T
Cuts at: 2592 2592
Size: 4136
PfoI T'CCnGG_A
Cuts at: 505 505
Size: 4136
PmeI GTTT'AAAC
Cuts at: 1135 1135
Size: 4136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 647 679 1070 1102 3275 3307 647
Size: 32 391 32 2173 32 1476
Fragments arranged by size:
2173 1476 391 32 32 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 681 713 681
Size: 32 4104
PstI C_TGCA'G
Cuts at: 1183 1868 1183
Size: 685 3451
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4136
PvuII CAG'CTG
Cuts at: 549 1121 1561 1921 549
Size: 572 440 360 2764
Fragments arranged by size:
2764 572 440 360
RsrII CG'GwC_CG
Cuts at: 2331 2331
Size: 4136
SacI G_AGCT'C
Cuts at: 1150 1150
Size: 4136
SalI G'TCGA_C
Cuts at: 1173 1173
Size: 4136
SapI GCTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
SbfI CC_TGCA'GG
Cuts at: 1183 1183
Size: 4136
ScaI AGT'ACT
Cuts at: 3653 3653
Size: 4136
SexAI A'CCwGG_T
Cuts at: 631 631
Size: 4136
SfcI C'TryA_G
Cuts at: 33 1179 1864 2857 3048 33
Size: 1146 685 993 191 1121
Fragments arranged by size:
1146 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1568 1816 1568
Size: 248 3888
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
SmaI CCC'GGG
Cuts at: 1158 1158
Size: 4136
SmlI C'TyrA_G
Cuts at: 601 1291 2206 2698 2960 601
Size: 690 915 492 262 1777
Fragments arranged by size:
1777 915 690 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4136
SphI G_CATG'C
Cuts at: 1189 1689 1189
Size: 500 3636
StyI C'CwwG_G
Cuts at: 107 1270 1624 107
Size: 1163 354 2619
Fragments arranged by size:
2619 1163 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1719 1852 2311 2520 1719
Size: 133 459 209 3335
Fragments arranged by size:
3335 459 209 133
TatI w'GTAC_w
Cuts at: 1095 3651 1095
Size: 2556 1580
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4104
Tth111I GACn'n_nGTC
Cuts at: 1933 1933
Size: 4136
XbaI T'CTAG_A
Cuts at: 1167 1167
Size: 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1631 1631
Size: 4136
XmaI C'CCGG_G
Cuts at: 1156 1156
Size: 4136
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI