Restriction map for
pF1KB4031
(Circular) (Six-base) MAPSORT of: pF1KB4031.seq Check: 4739 from: 1 to: 5483
pF1KB4031 5483 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1337 2521 1337
Size: 1184 4299
Acc65I G'GTAC_C
Cuts at: 2499 2499
Size: 5483
AclI AA'CG_TT
Cuts at: 5323 5323
Size: 5483
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 957 959 1644 1779 1967 2086 2176 3308
Size: 748 2 685 135 188 119 90 1132
Cuts at: 3308 3740 4093 4755 209
Size: 432 353 662 937
Fragments arranged by size:
1132 937 748 685 662 432 353 188
135 119 90 2
AflII C'TTAA_G
Cuts at: 312 312
Size: 5483
AflIII A'CryG_T
Cuts at: 1860 2058 2835 4635 4916 1860
Size: 198 777 1800 281 2427
Fragments arranged by size:
2427 1800 777 281 198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2480 2480
Size: 5483
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 836 868 836
Size: 32 5451
AlwNI CAG_nnn'CTG
Cuts at: 1758 2788 4226 1758
Size: 1030 1438 3015
Fragments arranged by size:
3015 1438 1030
ApaI G_GGCC'C
Cuts at: 810 810
Size: 5483
ApaLI G'TGCA_C
Cuts at: 4321 4321
Size: 5483
ApoI r'AATT_y
Cuts at: 415 558 2024 2487 4836 5092 415
Size: 143 1466 463 2349 256 806
Fragments arranged by size:
2349 1466 806 463 256 143
AseI AT'TA_AT
Cuts at: 20 1349 20
Size: 1329 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5483
AvaI C'yCGr_G
Cuts at: 1000 2503 1000
Size: 1503 3980
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 722 755 722
Size: 33 5450
BamHI G'GATC_C
Cuts at: 2508 2508
Size: 5483
BanI G'GyrC_C
Cuts at: 2499 2913 3161 3196 2499
Size: 414 248 35 4786
Fragments arranged by size:
4786 414 248 35
BanII G_rGCy'C
Cuts at: 810 2497 3527 810
Size: 1687 1030 2766
Fragments arranged by size:
2766 1687 1030
BbeI G_GCGC'C
Cuts at: 2917 3165 2917
Size: 248 5235
BbsI GAAGACnn'nnnn_
Cuts at: 1102 1815 1102
Size: 713 4770
BciVI GTATCCnnnnn_n'
Cuts at: 3381 4437 5483 3381
Size: 1056 1046 3381
Fragments arranged by size:
3381 1056 1046
BclI T'GATC_A
Cuts at: 3003 3003
Size: 5483
BfrBI ATG'CAT
Cuts at: 622 622
Size: 5483
BglI GCCn_nnn'nGGC
Cuts at: 2473 2473
Size: 5483
BglII A'GATC_T
Cuts at: 308 2354 2998 5035 308
Size: 2046 644 2037 756
Fragments arranged by size:
2046 2037 756 644
BlpI GC'TnA_GC
Cuts at: 2595 2595
Size: 5483
Bme1580I G_kGCm'C
Cuts at: 810 2469 3108 3201 4325 810
Size: 1659 639 93 1124 1968
Fragments arranged by size:
1968 1659 1124 639 93
BmrI ACTGGGnnnn_n'
Cuts at: 2695 2776 3111 5255 2695
Size: 81 335 2144 2923
Fragments arranged by size:
2923 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3850 3850
Size: 5483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1908 2422 1908
Size: 514 4969
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 2659 3538 4252 4550 2191
Size: 468 879 714 298 3124
Fragments arranged by size:
3124 879 714 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 5187 5187
Size: 5483
BsaAI yAC'GTr
Cuts at: 2836 2836
Size: 5483
BsaBI GATnn'nnATC
Cuts at: 6 2997 5034 6
Size: 2991 2037 455
Fragments arranged by size:
2991 2037 455
BsaHI Gr'CG_yC
Cuts at: 723 2914 3162 3864 5360 723
Size: 2191 248 702 1496 846
Fragments arranged by size:
2191 1496 846 702 248
BsaWI w'CCGG_w
Cuts at: 2672 3193 3871 4282 4429 4703 2672
Size: 521 678 411 147 274 3452
Fragments arranged by size:
3452 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 404 434 912 942 404
Size: 30 478 30 4945
Fragments arranged by size:
4945 478 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 920 1222 920
Size: 302 5181
BseYI C'CCAG_C
Cuts at: 1394 2908 3666 4331 4739 1394
Size: 1514 758 665 408 2138
Fragments arranged by size:
2138 1514 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1091 1091
Size: 5483
BsiEI CG_ry'CG
Cuts at: 104 3025 3071 4301 104
Size: 2921 46 1230 1286
Fragments arranged by size:
2921 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1803 2279 2497 3275 3465 4325 1803
Size: 476 218 778 190 860 2961
Fragments arranged by size:
2961 860 778 476 218 190
BsmI GAATG_Cn'
Cuts at: 393 1664 393
Size: 1271 4212
Bsp1286I G_dGCh'C
Cuts at: 810 1803 2279 2469 2497 3108 3201 3275 3465
Size: 993 476 190 28 611 93 74 190
Cuts at: 3465 3527 4325 810
Size: 62 798 1968
Fragments arranged by size:
1968 993 798 611 476 190 190 93
74 62 28
BspHI T'CATG_A
Cuts at: 1815 2261 3915 1815
Size: 446 1654 3383
Fragments arranged by size:
3383 1654 446
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1561 2533 2747 3049 3430 1561
Size: 972 214 302 381 3614
Fragments arranged by size:
3614 972 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3775 3829 5313 5478 50
Size: 3725 54 1484 165 55
Fragments arranged by size:
3725 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3395 3395
Size: 5483
BsrFI r'CCGG_y
Cuts at: 3481 3662 3481
Size: 181 5302
BsrGI T'GTAC_A
Cuts at: 325 1220 1749 325
Size: 895 529 4059
Fragments arranged by size:
4059 895 529
BssSI C'ACGA_G
Cuts at: 1994 3754 4462 1994
Size: 1760 708 3015
Fragments arranged by size:
3015 1760 708
BstAPI GCAn_nnn'nTGC
Cuts at: 909 2860 909
Size: 1951 3532
BstBI TT'CG_AA
Cuts at: 3844 3844
Size: 5483
BstEII G'GTnAC_C
Cuts at: 2394 2394
Size: 5483
BstYI r'GATC_y
Cuts at: 1 308 1139 1425 1973 2069 2354 2508 2975
Size: 307 831 286 548 96 285 154 467
Cuts at: 2975 2998 3333 3579 3885 3897 3983 5035 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 831 548 467 449 335 307 306
286 285 246 154 96 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 593 593
Size: 5483
BtgI C'CryG_G
Cuts at: 2320 2320
Size: 5483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3433 3614 3777 3433
Size: 181 163 5139
Fragments arranged by size:
5139 181 163
BtsI GCAGTG_nn'
Cuts at: 895 1062 2154 2417 2726 895
Size: 167 1092 263 309 3652
Fragments arranged by size:
3652 1092 309 263 167
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 390 425 390
Size: 35 5448
DraI TTT'AAA
Cuts at: 1632 2482 1632
Size: 850 4633
DrdI GACnn_nn'nnGTC
Cuts at: 3189 4533 3189
Size: 1344 4139
EaeI y'GGCC_r
Cuts at: 2444 3068 3242 3633 3660 5005 2444
Size: 624 174 391 27 1345 2922
Fragments arranged by size:
2922 1345 624 391 174 27
EagI C'GGCC_G
Cuts at: 3068 3068
Size: 5483
EarI CTCTTCn'nnn_
Cuts at: 779 1046 1595 1986 2419 3506 3716 779
Size: 267 549 391 433 1087 210 2546
Fragments arranged by size:
2546 1087 549 433 391 267 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3691 4433 4579 5293 3691
Size: 742 146 714 3881
Fragments arranged by size:
3881 742 714 146
EcoICRI GAG'CTC
Cuts at: 2495 2495
Size: 5483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 957 959 1644 1779 1908 1967 2086 2176
Size: 748 2 685 135 129 59 119 90
Cuts at: 2176 2422 3308 3740 4093 4755 209
Size: 246 886 432 353 662 937
Fragments arranged by size:
937 886 748 685 662 432 353 246
135 129 119 90 59 2
EcoO109I rG'GnC_Cy
Cuts at: 1006 2622 1006
Size: 1616 3867
EcoRI G'AATT_C
Cuts at: 2487 2487
Size: 5483
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 304 1075 1115 1161 2119 3502 304
Size: 771 40 46 958 1383 2285
Fragments arranged by size:
2285 1383 958 771 46 40
FspI TGC'GCA
Cuts at: 3264 3264
Size: 5483
HaeII r_GCGC'y
Cuts at: 2917 3165 4395 5166 2917
Size: 248 1230 771 3234
Fragments arranged by size:
3234 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1881 1913 3325 3355 3357 3387 1881
Size: 32 1412 30 2 30 3977
Fragments arranged by size:
3977 1412 32 30 30 2
HincII GTy'rAC
Cuts at: 439 2522 439
Size: 2083 3400
KasI G'GCGC_C
Cuts at: 2913 3161 2913
Size: 248 5235
KpnI G_GTAC'C
Cuts at: 2503 2503
Size: 5483
MluI A'CGCG_T
Cuts at: 4916 4916
Size: 5483
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 357 627 1369 4242 4426 357
Size: 270 742 2873 184 1414
Fragments arranged by size:
2873 1414 742 270 184
MscI TGG'CCA
Cuts at: 3244 3244
Size: 5483
MslI CAynn'nnrTG
Cuts at: 1211 1213 3472 3599 1211
Size: 2 2259 127 3095
Fragments arranged by size:
3095 2259 127 2
MspA1I CmG'CkG
Cuts at: 2253 2594 2908 3268 4052 4297 2253
Size: 341 314 360 784 245 3439
Fragments arranged by size:
3439 784 360 341 314 245
NaeI GCC'GGC
Cuts at: 3664 3664
Size: 5483
NarI GG'CG_CC
Cuts at: 2914 3162 2914
Size: 248 5235
NcoI C'CATG_G
Cuts at: 2320 2320
Size: 5483
NgoMIV G'CCGG_C
Cuts at: 3662 3662
Size: 5483
NsiI A_TGCA'T
Cuts at: 624 624
Size: 5483
NspI r_CATG'y
Cuts at: 1308 1864 2062 2536 3036 4639 1308
Size: 556 198 474 500 1603 2152
Fragments arranged by size:
2152 1603 556 500 474 198
PasI CC'CwG_GG
Cuts at: 641 641
Size: 5483
PciI A'CATG_T
Cuts at: 1860 2058 4635 1860
Size: 198 2577 2708
Fragments arranged by size:
2708 2577 198
PfoI T'CCnGG_A
Cuts at: 1593 1593
Size: 5483
PmeI GTTT'AAAC
Cuts at: 2482 2482
Size: 5483
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 3929
Size: 32 5451
PpuMI rG'GwC_Cy
Cuts at: 1006 1006
Size: 5483
PsiI TTA'TAA
Cuts at: 470 470
Size: 5483
PspOMI G'GGCC_C
Cuts at: 806 806
Size: 5483
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1445 1477 1445
Size: 32 5451
PstI C_TGCA'G
Cuts at: 902 2351 2474 2530 3215 902
Size: 1449 123 56 685 3170
Fragments arranged by size:
3170 1449 685 123 56
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5483
PvuII CAG'CTG
Cuts at: 2908 3268 2908
Size: 360 5123
RsrII CG'GwC_CG
Cuts at: 3678 3678
Size: 5483
SacI G_AGCT'C
Cuts at: 2497 2497
Size: 5483
SalI G'TCGA_C
Cuts at: 2520 2520
Size: 5483
SapI GCTCTTCn'nnn_
Cuts at: 3506 3716 3506
Size: 210 5273
SbfI CC_TGCA'GG
Cuts at: 2474 2530 2474
Size: 56 5427
ScaI AGT'ACT
Cuts at: 5000 5000
Size: 5483
SfcI C'TryA_G
Cuts at: 33 898 2347 2362 2470 2526 3211 4179 4370
Size: 865 1449 15 108 56 685 968 191
Cuts at: 4370 33
Size: 1146
Fragments arranged by size:
1449 1146 968 865 685 191 108 56
15
SfoI GGC'GCC
Cuts at: 2915 3163 2915
Size: 248 5235
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5483
SmaI CCC'GGG
Cuts at: 2505 2505
Size: 5483
SmlI C'TyrA_G
Cuts at: 312 2170 2638 3553 4267 4529 312
Size: 1858 468 915 714 262 1266
Fragments arranged by size:
1858 1266 915 714 468 262
SpeI A'CTAG_T
Cuts at: 67 1917 67
Size: 1850 3633
SphI G_CATG'C
Cuts at: 2536 3036 2536
Size: 500 4983
StyI C'CwwG_G
Cuts at: 961 2320 2617 2971 961
Size: 1359 297 354 3473
Fragments arranged by size:
3473 1359 354 297
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2287 3066 3199 3658 3867 2287
Size: 779 133 459 209 3903
Fragments arranged by size:
3903 779 459 209 133
TatI w'GTAC_w
Cuts at: 325 1220 1749 1914 2387 4998 325
Size: 895 529 165 473 2611 810
Fragments arranged by size:
2611 895 810 529 473 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 981 1013 1086 1118 5000 5032 981
Size: 32 73 32 3882 32 1432
Fragments arranged by size:
3882 1432 73 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3280 3280
Size: 5483
XbaI T'CTAG_A
Cuts at: 1464 2514 1464
Size: 1050 4433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2978 2978
Size: 5483
XmaI C'CCGG_G
Cuts at: 2503 2503
Size: 5483
XmnI GAAnn'nnTTC
Cuts at: 2189 2233 2189
Size: 44 5439
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PfoI PmeI PpiI PpuMI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AscI AvrII
BbvCI BcgI BmgBI BmtI Bpu10I BsiWI BsmBI BspEI
BssHII BstXI BstZ17I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PflMI PmlI PshAI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI