Restriction map for
pF1KB4028
(Circular) (Six-base) MAPSORT of: pF1KB4028.seq Check: 1648 from: 1 to: 4796
pF1KB4028 4796 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1834 1834
Size: 4796
Acc65I G'GTAC_C
Cuts at: 1812 1812
Size: 4796
AclI AA'CG_TT
Cuts at: 843 4636 843
Size: 3793 1003
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1115 1500 1517 2621 3053 3800 4068 1115
Size: 385 17 1104 432 747 268 1843
Fragments arranged by size:
1843 1104 747 432 385 268 17
AflIII A'CryG_T
Cuts at: 2148 3252 4229 2148
Size: 1104 977 2715
Fragments arranged by size:
2715 1104 977
AgeI A'CCGG_T
Cuts at: 251 3184 251
Size: 2933 1863
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1343 1375 1343
Size: 32 4764
AlwNI CAG_nnn'CTG
Cuts at: 162 1077 2101 3668 162
Size: 915 1024 1567 1290
Fragments arranged by size:
1567 1290 1024 915
ApaLI G'TGCA_C
Cuts at: 3566 3566
Size: 4796
ApoI r'AATT_y
Cuts at: 373 1418 1542 1618 1800 4149 4405 373
Size: 1045 124 76 182 2349 256 764
Fragments arranged by size:
2349 1045 764 256 182 124 76
AseI AT'TA_AT
Cuts at: 20 20
Size: 4796
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 1816 1816
Size: 4796
BamHI G'GATC_C
Cuts at: 815 1821 815
Size: 1006 3790
BanI G'GyrC_C
Cuts at: 1812 2226 2474 2509 1812
Size: 414 248 35 4099
Fragments arranged by size:
4099 414 248 35
BanII G_rGCy'C
Cuts at: 1810 2840 1810
Size: 1030 3766
BbeI G_GCGC'C
Cuts at: 2230 2478 2230
Size: 248 4548
BbsI GAAGACnn'nnnn_
Cuts at: 986 986
Size: 4796
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 274 308 274
Size: 34 4762
BciVI GTATCCnnnnn_n'
Cuts at: 217 480 959 2694 3455 4796 217
Size: 263 479 1735 761 1341 217
Fragments arranged by size:
1735 1341 761 479 263 217
BclI T'GATC_A
Cuts at: 2316 2316
Size: 4796
BfrBI ATG'CAT
Cuts at: 199 199
Size: 4796
BglII A'GATC_T
Cuts at: 2311 4348 2311
Size: 2037 2759
BlpI GC'TnA_GC
Cuts at: 1705 1908 1705
Size: 203 4593
Bme1580I G_kGCm'C
Cuts at: 2421 2514 3570 2421
Size: 93 1056 3647
Fragments arranged by size:
3647 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2008 2089 2424 4568 2008
Size: 81 335 2144 2236
Fragments arranged by size:
2236 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 4796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 940 1217 879
Size: 61 277 4458
Fragments arranged by size:
4458 277 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1972 2851 3343 3641 1972
Size: 879 492 298 3127
Fragments arranged by size:
3127 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4500 4500
Size: 4796
BsaAI yAC'GTr
Cuts at: 2149 2149
Size: 4796
BsaBI GATnn'nnATC
Cuts at: 6 1472 2310 4347 6
Size: 1466 838 2037 455
Fragments arranged by size:
2037 1466 838 455
BsaHI Gr'CG_yC
Cuts at: 2227 2475 3177 4673 2227
Size: 248 702 1496 2350
Fragments arranged by size:
2350 1496 702 248
BsaWI w'CCGG_w
Cuts at: 251 1985 2506 3184 3458 3605 4016 251
Size: 1734 521 678 274 147 411 1031
Fragments arranged by size:
1734 1031 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1282 1285 1282
Size: 3 4793
BseYI C'CCAG_C
Cuts at: 2221 2979 3556 4052 2221
Size: 758 577 496 2965
Fragments arranged by size:
2965 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 727
Size: 4796
BsiEI CG_ry'CG
Cuts at: 104 2338 2384 3592 104
Size: 2234 46 1208 1308
Fragments arranged by size:
2234 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1810 2588 2778 3570 1810
Size: 778 190 792 3036
Fragments arranged by size:
3036 792 778 190
BsmI GAATG_Cn'
Cuts at: 199 287 1067 199
Size: 88 780 3928
Fragments arranged by size:
3928 780 88
BsmBI CGTCTCn'nnnn_
Cuts at: 1450 1450
Size: 4796
Bsp1286I G_dGCh'C
Cuts at: 1810 2421 2514 2588 2778 2840 3570 1810
Size: 611 93 74 190 62 730 3036
Fragments arranged by size:
3036 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4016 4016
Size: 4796
BspHI T'CATG_A
Cuts at: 3972 3972
Size: 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1846 2060 2362 2743 1846
Size: 214 302 381 3899
Fragments arranged by size:
3899 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3088 3142 4626 4791 50
Size: 3038 54 1484 165 55
Fragments arranged by size:
3038 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 557 1601 2708 557
Size: 1044 1107 2645
Fragments arranged by size:
2645 1107 1044
BsrFI r'CCGG_y
Cuts at: 251 2794 2975 3184 251
Size: 2543 181 209 1863
Fragments arranged by size:
2543 1863 209 181
BssSI C'ACGA_G
Cuts at: 3067 3425 3067
Size: 358 4438
BstAPI GCAn_nnn'nTGC
Cuts at: 2173 2173
Size: 4796
BstBI TT'CG_AA
Cuts at: 3157 3157
Size: 4796
BstYI r'GATC_y
Cuts at: 1 815 1821 2288 2311 2646 2892 3904 3990
Size: 814 1006 467 23 335 246 1012 86
Cuts at: 3990 4002 4348 1
Size: 12 346 449
Fragments arranged by size:
1012 1006 814 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 811 811
Size: 4796
BtgI C'CryG_G
Cuts at: 701 1341 701
Size: 640 4156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2746 2927 3090 2746
Size: 181 163 4452
Fragments arranged by size:
4452 181 163
BtsI GCAGTG_nn'
Cuts at: 141 2039 141
Size: 1898 2898
ClaI AT'CG_AT
Cuts at: 1377 1377
Size: 4796
DraI TTT'AAA
Cuts at: 546 632 677 1795 546
Size: 86 45 1118 3547
Fragments arranged by size:
3547 1118 86 45
DrdI GACnn_nn'nnGTC
Cuts at: 2502 3360 2502
Size: 858 3938
EaeI y'GGCC_r
Cuts at: 2381 2555 2946 2973 4318 2381
Size: 174 391 27 1345 2859
Fragments arranged by size:
2859 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2381 2381
Size: 4796
EarI CTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3004 3314 3460 4606 3004
Size: 310 146 1146 3194
Fragments arranged by size:
3194 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1808 1808
Size: 4796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 940 1115 1217 1500 1517 2621 3053 3800
Size: 61 175 102 283 17 1104 432 747
Cuts at: 3800 4068 879
Size: 268 1607
Fragments arranged by size:
1607 1104 747 432 283 268 175 102
61 17
EcoO109I rG'GnC_Cy
Cuts at: 1935 1935
Size: 4796
EcoRI G'AATT_C
Cuts at: 1418 1542 1800 1418
Size: 124 258 4414
Fragments arranged by size:
4414 258 124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4796
FspI TGC'GCA
Cuts at: 2577 2577
Size: 4796
HaeII r_GCGC'y
Cuts at: 127 2230 2478 3500 4479 127
Size: 2103 248 1022 979 444
Fragments arranged by size:
2103 1022 979 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2638 2668 2670 2700 2638
Size: 30 2 30 4734
Fragments arranged by size:
4734 30 30 2
HincII GTy'rAC
Cuts at: 267 1835 267
Size: 1568 3228
KasI G'GCGC_C
Cuts at: 2226 2474 2226
Size: 248 4548
KpnI G_GTAC'C
Cuts at: 1816 1816
Size: 4796
MfeI C'AATT_G
Cuts at: 968 968
Size: 4796
MluI A'CGCG_T
Cuts at: 4229 4229
Size: 4796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 437 3467 3651 437
Size: 3030 184 1582
Fragments arranged by size:
3030 1582 184
MscI TGG'CCA
Cuts at: 2557 2557
Size: 4796
MslI CAynn'nnrTG
Cuts at: 419 2785 2912 419
Size: 2366 127 2303
Fragments arranged by size:
2366 2303 127
MspA1I CmG'CkG
Cuts at: 273 927 1780 1907 2221 2581 3594 3839 273
Size: 654 853 127 314 360 1013 245 1230
Fragments arranged by size:
1230 1013 853 654 360 314 245 127
NaeI GCC'GGC
Cuts at: 2977 2977
Size: 4796
NarI GG'CG_CC
Cuts at: 2227 2475 2227
Size: 248 4548
NcoI C'CATG_G
Cuts at: 1341 1341
Size: 4796
NdeI CA'TA_TG
Cuts at: 525 525
Size: 4796
NgoMIV G'CCGG_C
Cuts at: 2975 2975
Size: 4796
NsiI A_TGCA'T
Cuts at: 201 201
Size: 4796
NspI r_CATG'y
Cuts at: 1849 2349 3256 1849
Size: 500 907 3389
Fragments arranged by size:
3389 907 500
PciI A'CATG_T
Cuts at: 3252 3252
Size: 4796
PflMI CCAn_nnn'nTGG
Cuts at: 1429 1429
Size: 4796
PfoI T'CCnGG_A
Cuts at: 651 651
Size: 4796
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4764
PstI C_TGCA'G
Cuts at: 1843 2528 1843
Size: 685 4111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 927 1780 2221 2581 927
Size: 853 441 360 3142
Fragments arranged by size:
3142 853 441 360
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 1810 1810
Size: 4796
SalI G'TCGA_C
Cuts at: 1833 1833
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
SbfI CC_TGCA'GG
Cuts at: 1843 1843
Size: 4796
ScaI AGT'ACT
Cuts at: 4313 4313
Size: 4796
SfcI C'TryA_G
Cuts at: 33 1839 2524 3517 3708 33
Size: 1806 685 993 191 1121
Fragments arranged by size:
1806 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2228 2476 2228
Size: 248 4548
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SmaI CCC'GGG
Cuts at: 1818 1818
Size: 4796
SmlI C'TyrA_G
Cuts at: 1951 2866 3358 3620 1951
Size: 915 492 262 3127
Fragments arranged by size:
3127 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1849 2349 1849
Size: 500 4296
StyI C'CwwG_G
Cuts at: 1097 1341 1930 2284 1097
Size: 244 589 354 3609
Fragments arranged by size:
3609 589 354 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2379 2512 2971 3180 2379
Size: 133 459 209 3995
Fragments arranged by size:
3995 459 209 133
TatI w'GTAC_w
Cuts at: 942 4311 942
Size: 3369 1427
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4313 4345 4313
Size: 32 4764
Tth111I GACn'n_nGTC
Cuts at: 2593 2593
Size: 4796
XbaI T'CTAG_A
Cuts at: 1368 1827 1368
Size: 459 4337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2291 2291
Size: 4796
XmaI C'CCGG_G
Cuts at: 1816 1816
Size: 4796
XmnI GAAnn'nnTTC
Cuts at: 505 1067 1530 505
Size: 562 463 3771
Fragments arranged by size:
3771 562 463
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsrGI BssHII BstEII BstXI BstZ17I CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NheI
NotI NruI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI