Restriction map for
pF1KB4017
(Circular) (Six-base) MAPSORT of: pF1KB4017.seq Check: 6804 from: 1 to: 4505
pF1KB4017 4505 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1318 1318
Size: 4505
AccI GT'mk_AC
Cuts at: 1543 1543
Size: 4505
Acc65I G'GTAC_C
Cuts at: 1521 1521
Size: 4505
AclI AA'CG_TT
Cuts at: 4345 4345
Size: 4505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 1357 2330 2762 3509 3777 507
Size: 850 973 432 747 268 1235
Fragments arranged by size:
1235 973 850 747 432 268
AfeI AGC'GCT
Cuts at: 688 688
Size: 4505
AflIII A'CryG_T
Cuts at: 1193 1857 2961 3938 1193
Size: 664 1104 977 1760
Fragments arranged by size:
1760 1104 977 664
AgeI A'CCGG_T
Cuts at: 2893 2893
Size: 4505
AleI CACnn'nnGTG
Cuts at: 1186 1186
Size: 4505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 337 652 337
Size: 315 4190
AlwNI CAG_nnn'CTG
Cuts at: 297 423 1335 1810 3377 297
Size: 126 912 475 1567 1425
Fragments arranged by size:
1567 1425 912 475 126
ApaI G_GGCC'C
Cuts at: 193 193
Size: 4505
ApaLI G'TGCA_C
Cuts at: 3275 3275
Size: 4505
ApoI r'AATT_y
Cuts at: 1509 3858 4114 1509
Size: 2349 256 1900
Fragments arranged by size:
2349 1900 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
AvaI C'yCGr_G
Cuts at: 193 1235 1525 193
Size: 1042 290 3173
Fragments arranged by size:
3173 1042 290
BamHI G'GATC_C
Cuts at: 1530 1530
Size: 4505
BanI G'GyrC_C
Cuts at: 209 738 1521 1935 2183 2218 209
Size: 529 783 414 248 35 2496
Fragments arranged by size:
2496 783 529 414 248 35
BanII G_rGCy'C
Cuts at: 193 200 373 994 1382 1519 2549 193
Size: 7 173 621 388 137 1030 2149
Fragments arranged by size:
2149 1030 621 388 173 137 7
BbeI G_GCGC'C
Cuts at: 213 1939 2187 213
Size: 1726 248 2531
Fragments arranged by size:
2531 1726 248
BbsI GAAGACnn'nnnn_
Cuts at: 117 1252 117
Size: 1135 3370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 904 938 904
Size: 34 4471
BciVI GTATCCnnnnn_n'
Cuts at: 2403 3164 4505 2403
Size: 761 1341 2403
Fragments arranged by size:
2403 1341 761
BclI T'GATC_A
Cuts at: 1415 2025 1415
Size: 610 3895
BglI GCCn_nnn'nGGC
Cuts at: 302 1052 1165 1228 302
Size: 750 113 63 3579
Fragments arranged by size:
3579 750 113 63
BglII A'GATC_T
Cuts at: 428 671 2020 4057 428
Size: 243 1349 2037 876
Fragments arranged by size:
2037 1349 876 243
BlpI GC'TnA_GC
Cuts at: 797 1617 797
Size: 820 3685
Bme1580I G_kGCm'C
Cuts at: 193 743 2130 2223 3279 193
Size: 550 1387 93 1056 1419
Fragments arranged by size:
1419 1387 1056 550 93
BmgBI CAC'GTC
Cuts at: 1188 1316 1188
Size: 128 4377
BmrI ACTGGGnnnn_n'
Cuts at: 211 1717 1798 2133 4277 211
Size: 1506 81 335 2144 439
Fragments arranged by size:
2144 1506 439 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 4505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 1026 456
Size: 570 3935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1681 2560 3052 3350 1681
Size: 879 492 298 2836
Fragments arranged by size:
2836 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 145 4209 145
Size: 4064 441
BsaAI yAC'GTr
Cuts at: 1858 1858
Size: 4505
BsaBI GATnn'nnATC
Cuts at: 6 2019 4056 6
Size: 2013 2037 455
Fragments arranged by size:
2037 2013 455
BsaHI Gr'CG_yC
Cuts at: 210 1249 1936 2184 2886 4382 210
Size: 1039 687 248 702 1496 333
Fragments arranged by size:
1496 1039 702 687 333 248
BsaWI w'CCGG_w
Cuts at: 1151 1694 2215 2893 3167 3314 3725 1151
Size: 543 521 678 274 147 411 1931
Fragments arranged by size:
1931 678 543 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 403 433 466 496 1093 1123 403
Size: 30 33 30 597 30 3785
Fragments arranged by size:
3785 597 33 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 481 633 1117 1120 481
Size: 152 484 3 3866
Fragments arranged by size:
3866 484 152 3
BseYI C'CCAG_C
Cuts at: 742 823 1435 1930 2688 3265 3761 742
Size: 81 612 495 758 577 496 1486
Fragments arranged by size:
1486 758 612 577 496 495 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1095 1296 1095
Size: 201 4304
BsiEI CG_ry'CG
Cuts at: 104 312 735 2047 2093 3301 104
Size: 208 423 1312 46 1208 1308
Fragments arranged by size:
1312 1308 1208 423 208 46
BsiHKAI G_wGCw'C
Cuts at: 200 1382 1519 2297 2487 3279 200
Size: 1182 137 778 190 792 1426
Fragments arranged by size:
1426 1182 792 778 190 137
Bsp1286I G_dGCh'C
Cuts at: 193 200 373 743 994 1382 1519 2130 2223
Size: 7 173 370 251 388 137 611 93
Cuts at: 2223 2297 2487 2549 3279 193
Size: 74 190 62 730 1419
Fragments arranged by size:
1419 730 611 388 370 251 190 173
137 93 74 62 7
BspEI T'CCGG_A
Cuts at: 1151 3725 1151
Size: 2574 1931
BspHI T'CATG_A
Cuts at: 3681 3681
Size: 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 875 1318 1555 1769 2071 2452 875
Size: 443 237 214 302 381 2928
Fragments arranged by size:
2928 443 381 302 237 214
BsrBI CCG'CTC
Cuts at: 50 161 2797 2851 4335 4500 50
Size: 111 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 111 55 54
BsrDI GCAATG_nn'
Cuts at: 585 2417 585
Size: 1832 2673
BsrFI r'CCGG_y
Cuts at: 495 806 2503 2684 2893 495
Size: 311 1697 181 209 2107
Fragments arranged by size:
2107 1697 311 209 181
BssSI C'ACGA_G
Cuts at: 2776 3134 2776
Size: 358 4147
BstAPI GCAn_nnn'nTGC
Cuts at: 423 587 1882 423
Size: 164 1295 3046
Fragments arranged by size:
3046 1295 164
BstBI TT'CG_AA
Cuts at: 2866 2866
Size: 4505
BstEII G'GTnAC_C
Cuts at: 498 951 498
Size: 453 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 114 861 1171 114
Size: 747 310 3448
Fragments arranged by size:
3448 747 310
BstYI r'GATC_y
Cuts at: 1 428 671 1530 1997 2020 2355 2601 3613
Size: 427 243 859 467 23 335 246 1012
Cuts at: 3613 3699 3711 4057 1
Size: 86 12 346 449
Fragments arranged by size:
1012 859 467 449 427 346 335 246
243 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1123 1123
Size: 4505
BtgI C'CryG_G
Cuts at: 201 201
Size: 4505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2455 2636 2799 2455
Size: 181 163 4161
Fragments arranged by size:
4161 181 163
BtsI GCAGTG_nn'
Cuts at: 298 424 1748 298
Size: 126 1324 3055
Fragments arranged by size:
3055 1324 126
DraI TTT'AAA
Cuts at: 1504 1504
Size: 4505
DrdI GACnn_nn'nnGTC
Cuts at: 565 1470 2211 3069 565
Size: 905 741 858 2001
Fragments arranged by size:
2001 905 858 741
EaeI y'GGCC_r
Cuts at: 167 326 911 2090 2264 2655 2682 4027 167
Size: 159 585 1179 174 391 27 1345 645
Fragments arranged by size:
1345 1179 645 585 391 174 159 27
EagI C'GGCC_G
Cuts at: 2090 2090
Size: 4505
EarI CTCTTCn'nnn_
Cuts at: 139 772 2528 2738 139
Size: 633 1756 210 1906
Fragments arranged by size:
1906 1756 633 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2713 3023 3169 4315 2713
Size: 310 146 1146 2903
Fragments arranged by size:
2903 1146 310 146
EcoICRI GAG'CTC
Cuts at: 198 1380 1517 198
Size: 1182 137 3186
Fragments arranged by size:
3186 1182 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 507 1026 1357 2330 2762 3509 3777 456
Size: 51 519 331 973 432 747 268 1184
Fragments arranged by size:
1184 973 747 519 432 331 268 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1427 1427
Size: 4505
EcoO109I rG'GnC_Cy
Cuts at: 189 190 714 1207 1230 1432 1644 189
Size: 1 524 493 23 202 212 3050
Fragments arranged by size:
3050 524 493 212 202 23 1
EcoRI G'AATT_C
Cuts at: 1509 1509
Size: 4505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4505
FspI TGC'GCA
Cuts at: 2286 2286
Size: 4505
HaeII r_GCGC'y
Cuts at: 213 575 690 804 1939 2187 3209 4188 213
Size: 362 115 114 1135 248 1022 979 530
Fragments arranged by size:
1135 1022 979 530 362 248 115 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2347 2377 2379 2409 2347
Size: 30 2 30 4443
Fragments arranged by size:
4443 30 30 2
HincII GTy'rAC
Cuts at: 1544 1544
Size: 4505
KasI G'GCGC_C
Cuts at: 209 1935 2183 209
Size: 1726 248 2531
Fragments arranged by size:
2531 1726 248
KpnI G_GTAC'C
Cuts at: 1525 1525
Size: 4505
MfeI C'AATT_G
Cuts at: 927 927
Size: 4505
MluI A'CGCG_T
Cuts at: 3938 3938
Size: 4505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 284 3176 3360 284
Size: 2892 184 1429
Fragments arranged by size:
2892 1429 184
MscI TGG'CCA
Cuts at: 169 328 2266 169
Size: 159 1938 2408
Fragments arranged by size:
2408 1938 159
MslI CAynn'nnrTG
Cuts at: 1060 1186 2494 2621 1060
Size: 126 1308 127 2944
Fragments arranged by size:
2944 1308 127 126
MspA1I CmG'CkG
Cuts at: 375 389 827 959 1079 1091 1616 1930 2290
Size: 14 438 132 120 12 525 314 360
Cuts at: 2290 3303 3548 375
Size: 1013 245 1332
Fragments arranged by size:
1332 1013 525 438 360 314 245 132
120 14 12
NaeI GCC'GGC
Cuts at: 808 2686 808
Size: 1878 2627
NarI GG'CG_CC
Cuts at: 210 1936 2184 210
Size: 1726 248 2531
Fragments arranged by size:
2531 1726 248
NgoMIV G'CCGG_C
Cuts at: 806 2684 806
Size: 1878 2627
NspI r_CATG'y
Cuts at: 765 1368 1558 2058 2965 765
Size: 603 190 500 907 2305
Fragments arranged by size:
2305 907 603 500 190
PasI CC'CwG_GG
Cuts at: 1399 1399
Size: 4505
PciI A'CATG_T
Cuts at: 2961 2961
Size: 4505
PflMI CCAn_nnn'nTGG
Cuts at: 591 591
Size: 4505
PfoI T'CCnGG_A
Cuts at: 1322 1322
Size: 4505
PmeI GTTT'AAAC
Cuts at: 1504 1504
Size: 4505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 4473
PshAI GACnn'nnGTC
Cuts at: 1297 1297
Size: 4505
PspOMI G'GGCC_C
Cuts at: 189 189
Size: 4505
PstI C_TGCA'G
Cuts at: 188 268 419 428 1552 2237 188
Size: 80 151 9 1124 685 2456
Fragments arranged by size:
2456 1124 685 151 80 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4505
PvuII CAG'CTG
Cuts at: 827 959 1079 1930 2290 827
Size: 132 120 851 360 3042
Fragments arranged by size:
3042 851 360 132 120
RsrII CG'GwC_CG
Cuts at: 2700 2700
Size: 4505
SacI G_AGCT'C
Cuts at: 200 1382 1519 200
Size: 1182 137 3186
Fragments arranged by size:
3186 1182 137
SalI G'TCGA_C
Cuts at: 1542 1542
Size: 4505
SapI GCTCTTCn'nnn_
Cuts at: 772 2528 2738 772
Size: 1756 210 2539
Fragments arranged by size:
2539 1756 210
SbfI CC_TGCA'GG
Cuts at: 188 1552 188
Size: 1364 3141
ScaI AGT'ACT
Cuts at: 4022 4022
Size: 4505
SfcI C'TryA_G
Cuts at: 33 184 264 415 424 1548 2233 3226 3417
Size: 151 80 151 9 1124 685 993 191
Cuts at: 3417 33
Size: 1121
Fragments arranged by size:
1124 1121 993 685 191 151 151 80
9
SfiI GGCCn_nnn'nGGCC
Cuts at: 1228 1228
Size: 4505
SfoI GGC'GCC
Cuts at: 211 1937 2185 211
Size: 1726 248 2531
Fragments arranged by size:
2531 1726 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
SmaI CCC'GGG
Cuts at: 1527 1527
Size: 4505
SmlI C'TyrA_G
Cuts at: 1660 2575 3067 3329 1660
Size: 915 492 262 2836
Fragments arranged by size:
2836 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4505
SphI G_CATG'C
Cuts at: 765 1558 2058 765
Size: 793 500 3212
Fragments arranged by size:
3212 793 500
StuI AGG'CCT
Cuts at: 607 1168 607
Size: 561 3944
StyI C'CwwG_G
Cuts at: 350 602 1496 1639 1993 350
Size: 252 894 143 354 2862
Fragments arranged by size:
2862 894 354 252 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 472 890 1240 2088 2221 2680 2889 472
Size: 418 350 848 133 459 209 2088
Fragments arranged by size:
2088 848 459 418 350 209 133
TatI w'GTAC_w
Cuts at: 749 4020 749
Size: 3271 1234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 403 435 1093 1125 4022 4054 403
Size: 32 658 32 2897 32 854
Fragments arranged by size:
2897 854 658 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 226 2302 226
Size: 2076 2429
XbaI T'CTAG_A
Cuts at: 144 1536 144
Size: 1392 3113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1056 2000 1056
Size: 944 3561
XmaI C'CCGG_G
Cuts at: 1525 1525
Size: 4505
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbvCI
BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NcoI NdeI NheI NotI NruI NsiI PacI
PmlI PpuMI PsiI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI