Restriction map for
pF1KB4014
(Circular) (Six-base) MAPSORT of: pF1KB4014.seq Check: 6293 from: 1 to: 4520
pF1KB4014 4520 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 861 861
Size: 4520
AccI GT'mk_AC
Cuts at: 1558 1558
Size: 4520
Acc65I G'GTAC_C
Cuts at: 1035 1536 1035
Size: 501 4019
AclI AA'CG_TT
Cuts at: 4360 4360
Size: 4520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1115 1252 2345 2777 3130 3792 1115
Size: 137 1093 432 353 662 1843
Fragments arranged by size:
1843 1093 662 432 353 137
AflIII A'CryG_T
Cuts at: 1872 3672 3953 1872
Size: 1800 281 2439
Fragments arranged by size:
2439 1800 281
AleI CACnn'nnGTG
Cuts at: 1346 1346
Size: 4520
AlwNI CAG_nnn'CTG
Cuts at: 280 448 502 814 862 1825 3263 280
Size: 168 54 312 48 963 1438 1537
Fragments arranged by size:
1537 1438 963 312 168 54 48
ApaLI G'TGCA_C
Cuts at: 3358 3358
Size: 4520
ApoI r'AATT_y
Cuts at: 1357 1524 3873 4129 1357
Size: 167 2349 256 1748
Fragments arranged by size:
2349 1748 256 167
AseI AT'TA_AT
Cuts at: 20 20
Size: 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
AvaI C'yCGr_G
Cuts at: 1540 1540
Size: 4520
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 260 293 260
Size: 33 4487
BamHI G'GATC_C
Cuts at: 1545 1545
Size: 4520
BanI G'GyrC_C
Cuts at: 405 1035 1087 1421 1536 1950 2198 2233 405
Size: 630 52 334 115 414 248 35 2692
Fragments arranged by size:
2692 630 414 334 248 115 52 35
BanII G_rGCy'C
Cuts at: 496 1081 1534 2564 496
Size: 585 453 1030 2452
Fragments arranged by size:
2452 1030 585 453
BbeI G_GCGC'C
Cuts at: 1425 1954 2202 1425
Size: 529 248 3743
Fragments arranged by size:
3743 529 248
BciVI GTATCCnnnnn_n'
Cuts at: 2418 3474 4520 2418
Size: 1056 1046 2418
Fragments arranged by size:
2418 1056 1046
BclI T'GATC_A
Cuts at: 2040 2040
Size: 4520
BglI GCCn_nnn'nGGC
Cuts at: 1085 1085
Size: 4520
BglII A'GATC_T
Cuts at: 2035 4072 2035
Size: 2037 2483
BlpI GC'TnA_GC
Cuts at: 1632 1632
Size: 4520
Bme1580I G_kGCm'C
Cuts at: 1003 1369 2145 2238 3362 1003
Size: 366 776 93 1124 2161
Fragments arranged by size:
2161 1124 776 366 93
BmrI ACTGGGnnnn_n'
Cuts at: 490 1732 1813 2148 4292 490
Size: 1242 81 335 2144 718
Fragments arranged by size:
2144 1242 718 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 4520
Bpu10I CC'TnA_GC
Cuts at: 524 524
Size: 4520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 1696 2575 3289 3587 371
Size: 1325 879 714 298 1304
Fragments arranged by size:
1325 1304 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 806 849 4224 806
Size: 43 3375 1102
Fragments arranged by size:
3375 1102 43
BsaAI yAC'GTr
Cuts at: 1873 1873
Size: 4520
BsaBI GATnn'nnATC
Cuts at: 6 2034 4071 6
Size: 2028 2037 455
Fragments arranged by size:
2037 2028 455
BsaHI Gr'CG_yC
Cuts at: 1422 1951 2199 2901 4397 1422
Size: 529 248 702 1496 1545
Fragments arranged by size:
1545 1496 702 529 248
BsaWI w'CCGG_w
Cuts at: 1068 1709 2230 2908 3319 3466 3740 1068
Size: 641 521 678 411 147 274 1848
Fragments arranged by size:
1848 678 641 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1488 1518 1488
Size: 30 4490
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 944 1139 1407 944
Size: 195 268 4057
Fragments arranged by size:
4057 268 195
BseYI C'CCAG_C
Cuts at: 336 560 946 958 1040 1945 2703 3368 3776
Size: 224 386 12 82 905 758 665 408
Cuts at: 3776 336
Size: 1080
Fragments arranged by size:
1080 905 758 665 408 386 224 82
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 1072 737
Size: 335 4185
BsiEI CG_ry'CG
Cuts at: 104 2062 2108 3338 104
Size: 1958 46 1230 1286
Fragments arranged by size:
1958 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1534 2312 2502 3362 1534
Size: 778 190 860 2692
Fragments arranged by size:
2692 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 778 778
Size: 4520
Bsp1286I G_dGCh'C
Cuts at: 496 1003 1081 1369 1534 2145 2238 2312 2502
Size: 507 78 288 165 611 93 74 190
Cuts at: 2502 2564 3362 496
Size: 62 798 1654
Fragments arranged by size:
1654 798 611 507 288 190 165 93
78 74 62
BspHI T'CATG_A
Cuts at: 1388 2952 1388
Size: 1564 2956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 589 788 861 1570 1784 2086 2467 589
Size: 199 73 709 214 302 381 2642
Fragments arranged by size:
2642 709 381 302 214 199 73
BsrBI CCG'CTC
Cuts at: 50 2812 2866 4350 4515 50
Size: 2762 54 1484 165 55
Fragments arranged by size:
2762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 461 1103 1481 2432 461
Size: 642 378 951 2549
Fragments arranged by size:
2549 951 642 378
BsrFI r'CCGG_y
Cuts at: 885 1379 2518 2699 885
Size: 494 1139 181 2706
Fragments arranged by size:
2706 1139 494 181
BsrGI T'GTAC_A
Cuts at: 423 423
Size: 4520
BssSI C'ACGA_G
Cuts at: 1242 2791 3499 1242
Size: 1549 708 2263
Fragments arranged by size:
2263 1549 708
BstAPI GCAn_nnn'nTGC
Cuts at: 751 862 1897 751
Size: 111 1035 3374
Fragments arranged by size:
3374 1035 111
BstBI TT'CG_AA
Cuts at: 2881 2881
Size: 4520
BstYI r'GATC_y
Cuts at: 1 700 1545 2012 2035 2370 2616 2922 2934
Size: 699 845 467 23 335 246 306 12
Cuts at: 2934 3020 4072 1
Size: 86 1052 449
Fragments arranged by size:
1052 845 699 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4520
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2470 2651 2814 2470
Size: 181 163 4176
Fragments arranged by size:
4176 181 163
BtsI GCAGTG_nn'
Cuts at: 1763 1763
Size: 4520
DraI TTT'AAA
Cuts at: 1218 1519 1218
Size: 301 4219
DrdI GACnn_nn'nnGTC
Cuts at: 2226 3570 2226
Size: 1344 3176
EaeI y'GGCC_r
Cuts at: 2105 2279 2670 2697 4042 2105
Size: 174 391 27 1345 2583
Fragments arranged by size:
2583 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2105 2105
Size: 4520
EarI CTCTTCn'nnn_
Cuts at: 290 2543 2753 290
Size: 2253 210 2057
Fragments arranged by size:
2253 2057 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2728 3470 3616 4330 2728
Size: 742 146 714 2918
Fragments arranged by size:
2918 742 714 146
EcoICRI GAG'CTC
Cuts at: 1532 1532
Size: 4520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1115 1252 2345 2777 3130 3792 1115
Size: 137 1093 432 353 662 1843
Fragments arranged by size:
1843 1093 662 432 353 137
EcoO109I rG'GnC_Cy
Cuts at: 1659 1659
Size: 4520
EcoRI G'AATT_C
Cuts at: 1524 1524
Size: 4520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1230 2539 1230
Size: 1309 3211
FspI TGC'GCA
Cuts at: 2301 2301
Size: 4520
HaeII r_GCGC'y
Cuts at: 415 1022 1425 1954 2202 3432 4203 415
Size: 607 403 529 248 1230 771 732
Fragments arranged by size:
1230 771 732 607 529 403 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1161 1193 1306 1338 2362 2392 2394 2424 1161
Size: 32 113 32 1024 30 2 30 3257
Fragments arranged by size:
3257 1024 113 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1559 1559
Size: 4520
KasI G'GCGC_C
Cuts at: 1421 1950 2198 1421
Size: 529 248 3743
Fragments arranged by size:
3743 529 248
KpnI G_GTAC'C
Cuts at: 1039 1540 1039
Size: 501 4019
MluI A'CGCG_T
Cuts at: 3953 3953
Size: 4520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 208 1519 3279 3463 208
Size: 1311 1760 184 1265
Fragments arranged by size:
1760 1311 1265 184
MscI TGG'CCA
Cuts at: 2281 2281
Size: 4520
MslI CAynn'nnrTG
Cuts at: 647 1030 1346 2509 2636 647
Size: 383 316 1163 127 2531
Fragments arranged by size:
2531 1163 383 316 127
MspA1I CmG'CkG
Cuts at: 553 685 1274 1631 1945 2305 3089 3334 553
Size: 132 589 357 314 360 784 245 1739
Fragments arranged by size:
1739 784 589 360 357 314 245 132
NaeI GCC'GGC
Cuts at: 2701 2701
Size: 4520
NarI GG'CG_CC
Cuts at: 1422 1951 2199 1422
Size: 529 248 3743
Fragments arranged by size:
3743 529 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4520
NgoMIV G'CCGG_C
Cuts at: 2699 2699
Size: 4520
NspI r_CATG'y
Cuts at: 1417 1573 2073 3676 1417
Size: 156 500 1603 2261
Fragments arranged by size:
2261 1603 500 156
PciI A'CATG_T
Cuts at: 3672 3672
Size: 4520
PflMI CCAn_nnn'nTGG
Cuts at: 1201 1201
Size: 4520
PfoI T'CCnGG_A
Cuts at: 160 309 160
Size: 149 4371
PmeI GTTT'AAAC
Cuts at: 1519 1519
Size: 4520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 4488
PshAI GACnn'nnGTC
Cuts at: 937 937
Size: 4520
PstI C_TGCA'G
Cuts at: 177 226 799 858 867 1567 2252 177
Size: 49 573 59 9 700 685 2445
Fragments arranged by size:
2445 700 685 573 59 49 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4520
PvuII CAG'CTG
Cuts at: 553 1274 1945 2305 553
Size: 721 671 360 2768
Fragments arranged by size:
2768 721 671 360
RsrII CG'GwC_CG
Cuts at: 2715 2715
Size: 4520
SacI G_AGCT'C
Cuts at: 1534 1534
Size: 4520
SalI G'TCGA_C
Cuts at: 1557 1557
Size: 4520
SapI GCTCTTCn'nnn_
Cuts at: 2543 2753 2543
Size: 210 4310
SbfI CC_TGCA'GG
Cuts at: 1567 1567
Size: 4520
ScaI AGT'ACT
Cuts at: 4037 4037
Size: 4520
SfcI C'TryA_G
Cuts at: 33 173 197 222 795 854 863 1185 1275
Size: 140 24 25 573 59 9 322 90
Cuts at: 1275 1563 2248 3216 3407 33
Size: 288 685 968 191 1146
Fragments arranged by size:
1146 968 685 573 322 288 191 140
90 59 25 24 9
SfoI GGC'GCC
Cuts at: 1423 1952 2200 1423
Size: 529 248 3743
Fragments arranged by size:
3743 529 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
SmaI CCC'GGG
Cuts at: 1542 1542
Size: 4520
SmlI C'TyrA_G
Cuts at: 386 1675 2590 3304 3566 386
Size: 1289 915 714 262 1340
Fragments arranged by size:
1340 1289 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4520
SphI G_CATG'C
Cuts at: 1417 1573 2073 1417
Size: 156 500 3864
Fragments arranged by size:
3864 500 156
StyI C'CwwG_G
Cuts at: 107 1654 2008 107
Size: 1547 354 2619
Fragments arranged by size:
2619 1547 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1208 2103 2236 2695 2904 1208
Size: 895 133 459 209 2824
Fragments arranged by size:
2824 895 459 209 133
TatI w'GTAC_w
Cuts at: 423 4035 423
Size: 3612 908
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1275 1307 4037 4069 1275
Size: 32 2730 32 1726
Fragments arranged by size:
2730 1726 32 32
Tth111I GACn'n_nGTC
Cuts at: 2317 2317
Size: 4520
XbaI T'CTAG_A
Cuts at: 1551 1551
Size: 4520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1198 2015 1198
Size: 817 3703
XmaI C'CCGG_G
Cuts at: 1540 1540
Size: 4520
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI BsiWI BsmI BspEI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI