Restriction map for
pF1KB3987
(Circular) (Six-base) MAPSORT of: pF1KB3987.seq Check: 2075 from: 1 to: 4256
pF1KB3987 4256 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 1088 1088
Size: 4256
AccI GT'mk_AC
Cuts at: 1294 1294
Size: 4256
Acc65I G'GTAC_C
Cuts at: 1272 1272
Size: 4256
AclI AA'CG_TT
Cuts at: 4096 4096
Size: 4256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1041 1252 2081 2513 2866 3528 1041
Size: 211 829 432 353 662 1769
Fragments arranged by size:
1769 829 662 432 353 211
AfeI AGC'GCT
Cuts at: 1093 1093
Size: 4256
AflIII A'CryG_T
Cuts at: 1608 3408 3689 1608
Size: 1800 281 2175
Fragments arranged by size:
2175 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 137 1561 2999 137
Size: 1424 1438 1394
Fragments arranged by size:
1438 1424 1394
ApaI G_GGCC'C
Cuts at: 168 168
Size: 4256
ApaLI G'TGCA_C
Cuts at: 3094 3094
Size: 4256
ApoI r'AATT_y
Cuts at: 1260 3609 3865 1260
Size: 2349 256 1651
Fragments arranged by size:
2349 1651 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4256
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
AvaI C'yCGr_G
Cuts at: 1276 1276
Size: 4256
BamHI G'GATC_C
Cuts at: 1281 1281
Size: 4256
BanI G'GyrC_C
Cuts at: 272 1272 1686 1934 1969 272
Size: 1000 414 248 35 2559
Fragments arranged by size:
2559 1000 414 248 35
BanII G_rGCy'C
Cuts at: 168 421 1270 2300 168
Size: 253 849 1030 2124
Fragments arranged by size:
2124 1030 849 253
BbeI G_GCGC'C
Cuts at: 276 1690 1938 276
Size: 1414 248 2594
Fragments arranged by size:
2594 1414 248
BbsI GAAGACnn'nnnn_
Cuts at: 1030 1030
Size: 4256
BbvCI CC'TCA_GC
Cuts at: 130 130
Size: 4256
BciVI GTATCCnnnnn_n'
Cuts at: 2154 3210 4256 2154
Size: 1056 1046 2154
Fragments arranged by size:
2154 1056 1046
BclI T'GATC_A
Cuts at: 1776 1776
Size: 4256
BglI GCCn_nnn'nGGC
Cuts at: 163 163
Size: 4256
BglII A'GATC_T
Cuts at: 1771 3808 1771
Size: 2037 2219
BlpI GC'TnA_GC
Cuts at: 1368 1368
Size: 4256
Bme1580I G_kGCm'C
Cuts at: 168 1881 1974 3098 168
Size: 1713 93 1124 1326
Fragments arranged by size:
1713 1326 1124 93
BmgBI CAC'GTC
Cuts at: 845 845
Size: 4256
BmrI ACTGGGnnnn_n'
Cuts at: 1468 1549 1884 4028 1468
Size: 81 335 2144 1696
Fragments arranged by size:
2144 1696 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 600 852 1099 400
Size: 200 252 247 3557
Fragments arranged by size:
3557 252 247 200
Bpu10I CC'TnA_GC
Cuts at: 130 568 130
Size: 438 3818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 397 405 1432 2311 3025 3323 313
Size: 84 8 1027 879 714 298 1246
Fragments arranged by size:
1246 1027 879 714 298 84 8
BsaI GGTCTCn'nnnn_
Cuts at: 867 934 3960 867
Size: 67 3026 1163
Fragments arranged by size:
3026 1163 67
BsaAI yAC'GTr
Cuts at: 1609 1609
Size: 4256
BsaBI GATnn'nnATC
Cuts at: 6 340 1770 3807 6
Size: 334 1430 2037 455
Fragments arranged by size:
2037 1430 455 334
BsaHI Gr'CG_yC
Cuts at: 273 1085 1687 1935 2637 4133 273
Size: 812 602 248 702 1496 396
Fragments arranged by size:
1496 812 702 602 396 248
BsaWI w'CCGG_w
Cuts at: 668 1445 1966 2644 3055 3202 3476 668
Size: 777 521 678 411 147 274 1448
Fragments arranged by size:
1448 777 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 855 855
Size: 4256
BseYI C'CCAG_C
Cuts at: 294 630 806 1681 2439 3104 3512 294
Size: 336 176 875 758 665 408 1038
Fragments arranged by size:
1038 875 758 665 408 336 176
BsiEI CG_ry'CG
Cuts at: 104 152 354 1798 1844 3074 104
Size: 48 202 1444 46 1230 1286
Fragments arranged by size:
1444 1286 1230 202 48 46
BsiHKAI G_wGCw'C
Cuts at: 1270 2048 2238 3098 1270
Size: 778 190 860 2428
Fragments arranged by size:
2428 860 778 190
BsmI GAATG_Cn'
Cuts at: 455 455
Size: 4256
BsmBI CGTCTCn'nnnn_
Cuts at: 978 1116 978
Size: 138 4118
Bsp1286I G_dGCh'C
Cuts at: 168 421 1270 1881 1974 2048 2238 2300 3098
Size: 253 849 611 93 74 190 62 798
Cuts at: 3098 168
Size: 1326
Fragments arranged by size:
1326 849 798 611 253 190 93 74
62
BspHI T'CATG_A
Cuts at: 2688 2688
Size: 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1239 1306 1520 1822 2203 1239
Size: 67 214 302 381 3292
Fragments arranged by size:
3292 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 258 311 2548 2602 4086 4251 50
Size: 208 53 2237 54 1484 165 55
Fragments arranged by size:
2237 1484 208 165 55 54 53
BsrDI GCAATG_nn'
Cuts at: 2168 2168
Size: 4256
BsrFI r'CCGG_y
Cuts at: 147 157 537 911 2254 2435 147
Size: 10 380 374 1343 181 1968
Fragments arranged by size:
1968 1343 380 374 181 10
BssHII G'CGCG_C
Cuts at: 191 193 261 191
Size: 2 68 4186
Fragments arranged by size:
4186 68 2
BssSI C'ACGA_G
Cuts at: 440 2527 3235 440
Size: 2087 708 1461
Fragments arranged by size:
2087 1461 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1633 1633
Size: 4256
BstBI TT'CG_AA
Cuts at: 2617 2617
Size: 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 915 915
Size: 4256
BstYI r'GATC_y
Cuts at: 1 284 767 1281 1748 1771 2106 2352 2658
Size: 283 483 514 467 23 335 246 306
Cuts at: 2658 2670 2756 3808 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 514 483 467 449 335 306 283
246 86 23 12
BtgI C'CryG_G
Cuts at: 348 348
Size: 4256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2206 2387 2550 2206
Size: 181 163 3912
Fragments arranged by size:
3912 181 163
BtsI GCAGTG_nn'
Cuts at: 1499 1499
Size: 4256
DraI TTT'AAA
Cuts at: 1255 1255
Size: 4256
DrdI GACnn_nn'nnGTC
Cuts at: 1962 3306 1962
Size: 1344 2912
EaeI y'GGCC_r
Cuts at: 149 351 539 675 795 1841 2015 2406 2433
Size: 202 188 136 120 1046 174 391 27
Cuts at: 2433 3778 149
Size: 1345 627
Fragments arranged by size:
1345 1046 627 391 202 188 174 136
120 27
EagI C'GGCC_G
Cuts at: 149 351 1841 149
Size: 202 1490 2564
Fragments arranged by size:
2564 1490 202
EarI CTCTTCn'nnn_
Cuts at: 967 991 1114 1150 2279 2489 967
Size: 24 123 36 1129 210 2734
Fragments arranged by size:
2734 1129 210 123 36 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2464 3206 3352 4066 2464
Size: 742 146 714 2654
Fragments arranged by size:
2654 742 714 146
EcoICRI GAG'CTC
Cuts at: 1268 1268
Size: 4256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 600 852 1041 1099 1252 2081 2513 2866
Size: 200 252 189 58 153 829 432 353
Cuts at: 2866 3528 400
Size: 662 1128
Fragments arranged by size:
1128 829 662 432 353 252 200 189
153 58
EcoNI CCTnn'n_nnAGG
Cuts at: 892 892
Size: 4256
EcoO109I rG'GnC_Cy
Cuts at: 164 885 1395 164
Size: 721 510 3025
Fragments arranged by size:
3025 721 510
EcoRI G'AATT_C
Cuts at: 1260 1260
Size: 4256
EcoRV GAT'ATC
Cuts at: 708 708
Size: 4256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 789 981 1062 1161 2275 789
Size: 192 81 99 1114 2770
Fragments arranged by size:
2770 1114 192 99 81
FspI TGC'GCA
Cuts at: 340 2037 340
Size: 1697 2559
FspAI rTGC'GCAy
Cuts at: 340 340
Size: 4256
HaeII r_GCGC'y
Cuts at: 276 376 813 1095 1690 1938 3168 3939 276
Size: 100 437 282 595 248 1230 771 593
Fragments arranged by size:
1230 771 595 593 437 282 248 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2098 2128 2130 2160 2098
Size: 30 2 30 4194
Fragments arranged by size:
4194 30 30 2
HincII GTy'rAC
Cuts at: 951 1295 951
Size: 344 3912
HindIII A'AGCT_T
Cuts at: 448 448
Size: 4256
KasI G'GCGC_C
Cuts at: 272 1686 1934 272
Size: 1414 248 2594
Fragments arranged by size:
2594 1414 248
KpnI G_GTAC'C
Cuts at: 1276 1276
Size: 4256
MluI A'CGCG_T
Cuts at: 3689 3689
Size: 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3015 3199 3015
Size: 184 4072
MscI TGG'CCA
Cuts at: 2017 2017
Size: 4256
MslI CAynn'nnrTG
Cuts at: 2245 2372 2245
Size: 127 4129
MspA1I CmG'CkG
Cuts at: 137 188 552 630 681 801 1367 1681 2041
Size: 51 364 78 51 120 566 314 360
Cuts at: 2041 2825 3070 137
Size: 784 245 1323
Fragments arranged by size:
1323 784 566 364 360 314 245 120
78 51 51
NaeI GCC'GGC
Cuts at: 149 159 539 2437 149
Size: 10 380 1898 1968
Fragments arranged by size:
1968 1898 380 10
NarI GG'CG_CC
Cuts at: 273 1687 1935 273
Size: 1414 248 2594
Fragments arranged by size:
2594 1414 248
NgoMIV G'CCGG_C
Cuts at: 147 157 537 2435 147
Size: 10 380 1898 1968
Fragments arranged by size:
1968 1898 380 10
NspI r_CATG'y
Cuts at: 1309 1809 3412 1309
Size: 500 1603 2153
Fragments arranged by size:
2153 1603 500
PciI A'CATG_T
Cuts at: 3408 3408
Size: 4256
PfoI T'CCnGG_A
Cuts at: 653 1049 653
Size: 396 3860
PmeI GTTT'AAAC
Cuts at: 1255 1255
Size: 4256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2702 2734 2702
Size: 32 4224
PpuMI rG'GwC_Cy
Cuts at: 885 885
Size: 4256
PshAI GACnn'nnGTC
Cuts at: 946 946
Size: 4256
PspOMI G'GGCC_C
Cuts at: 164 164
Size: 4256
PstI C_TGCA'G
Cuts at: 1223 1250 1303 1988 1223
Size: 27 53 685 3491
Fragments arranged by size:
3491 685 53 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4256
PvuII CAG'CTG
Cuts at: 552 630 681 1681 2041 552
Size: 78 51 1000 360 2767
Fragments arranged by size:
2767 1000 360 78 51
RsrII CG'GwC_CG
Cuts at: 267 2451 267
Size: 2184 2072
SacI G_AGCT'C
Cuts at: 1270 1270
Size: 4256
SalI G'TCGA_C
Cuts at: 1293 1293
Size: 4256
SapI GCTCTTCn'nnn_
Cuts at: 967 2279 2489 967
Size: 1312 210 2734
Fragments arranged by size:
2734 1312 210
SbfI CC_TGCA'GG
Cuts at: 1223 1303 1223
Size: 80 4176
ScaI AGT'ACT
Cuts at: 649 3773 649
Size: 3124 1132
SexAI A'CCwGG_T
Cuts at: 881 1097 881
Size: 216 4040
SfcI C'TryA_G
Cuts at: 33 1219 1246 1299 1984 2952 3143 33
Size: 1186 27 53 685 968 191 1146
Fragments arranged by size:
1186 1146 968 685 191 53 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 163 163
Size: 4256
SfoI GGC'GCC
Cuts at: 274 1688 1936 274
Size: 1414 248 2594
Fragments arranged by size:
2594 1414 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
SmaI CCC'GGG
Cuts at: 1278 1278
Size: 4256
SmlI C'TyrA_G
Cuts at: 328 412 420 1411 2326 3040 3302 328
Size: 84 8 991 915 714 262 1282
Fragments arranged by size:
1282 991 915 714 262 84 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4256
SphI G_CATG'C
Cuts at: 1309 1809 1309
Size: 500 3756
StyI C'CwwG_G
Cuts at: 460 731 1390 1744 460
Size: 271 659 354 2972
Fragments arranged by size:
2972 659 354 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1839 1972 2431 2640 1839
Size: 133 459 209 3455
Fragments arranged by size:
3455 459 209 133
TatI w'GTAC_w
Cuts at: 647 3771 647
Size: 3124 1132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3773 3805 3773
Size: 32 4224
Tth111I GACn'n_nGTC
Cuts at: 883 2053 883
Size: 1170 3086
XbaI T'CTAG_A
Cuts at: 1287 1287
Size: 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 738 855 915 1751 114
Size: 624 117 60 836 2619
Fragments arranged by size:
2619 836 624 117 60
XmaI C'CCGG_G
Cuts at: 1276 1276
Size: 4256
XmnI GAAnn'nnTTC
Cuts at: 649 820 934 1045 649
Size: 171 114 111 3860
Fragments arranged by size:
3860 171 114 111
ZraI GAC'GTC
Cuts at: 1086 1086
Size: 4256
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsgI BsiWI
BspEI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
FseI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PsiI PspXI PsrI
SacII SanDI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI