Restriction map for
pF1KB3985
(Circular) (Six-base) MAPSORT of: pF1KB3985.seq Check: 1773 from: 1 to: 4121
pF1KB3985 4121 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 662 1159 662
Size: 497 3624
Acc65I G'GTAC_C
Cuts at: 1137 1137
Size: 4121
AclI AA'CG_TT
Cuts at: 3961 3961
Size: 4121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 887 1946 2378 3125 3393 724
Size: 163 1059 432 747 268 1452
Fragments arranged by size:
1452 1059 747 432 268 163
AfeI AGC'GCT
Cuts at: 149 149
Size: 4121
AflIII A'CryG_T
Cuts at: 1473 2577 3554 1473
Size: 1104 977 2040
Fragments arranged by size:
2040 1104 977
AgeI A'CCGG_T
Cuts at: 2509 2509
Size: 4121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 652 652
Size: 4121
AlwNI CAG_nnn'CTG
Cuts at: 392 693 1426 2993 392
Size: 301 733 1567 1520
Fragments arranged by size:
1567 1520 733 301
ApaI G_GGCC'C
Cuts at: 625 625
Size: 4121
ApaLI G'TGCA_C
Cuts at: 162 2891 162
Size: 2729 1392
ApoI r'AATT_y
Cuts at: 1125 3474 3730 1125
Size: 2349 256 1516
Fragments arranged by size:
2349 1516 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4121
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
AvaI C'yCGr_G
Cuts at: 310 1141 310
Size: 831 3290
BamHI G'GATC_C
Cuts at: 560 888 1146 560
Size: 328 258 3535
Fragments arranged by size:
3535 328 258
BanI G'GyrC_C
Cuts at: 601 867 1137 1551 1799 1834 601
Size: 266 270 414 248 35 2888
Fragments arranged by size:
2888 414 270 266 248 35
BanII G_rGCy'C
Cuts at: 625 752 839 1135 2165 625
Size: 127 87 296 1030 2581
Fragments arranged by size:
2581 1030 296 127 87
BbeI G_GCGC'C
Cuts at: 1555 1803 1555
Size: 248 3873
BbsI GAAGACnn'nnnn_
Cuts at: 118 118
Size: 4121
BciVI GTATCCnnnnn_n'
Cuts at: 337 2019 2780 4121 337
Size: 1682 761 1341 337
Fragments arranged by size:
1682 1341 761 337
BclI T'GATC_A
Cuts at: 1641 1641
Size: 4121
BglI GCCn_nnn'nGGC
Cuts at: 197 197
Size: 4121
BglII A'GATC_T
Cuts at: 1636 3673 1636
Size: 2037 2084
BlpI GC'TnA_GC
Cuts at: 799 1233 799
Size: 434 3687
Bme1580I G_kGCm'C
Cuts at: 166 399 604 625 872 1032 1746 1839 2895
Size: 233 205 21 247 160 714 93 1056
Cuts at: 2895 166
Size: 1392
Fragments arranged by size:
1392 1056 714 247 233 205 160 93
21
BmgBI CAC'GTC
Cuts at: 510 510
Size: 4121
BmrI ACTGGGnnnn_n'
Cuts at: 1035 1333 1414 1749 3893 1035
Size: 298 81 335 2144 1263
Fragments arranged by size:
2144 1263 335 298 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 853
Size: 4121
Bpu10I CC'TnA_GC
Cuts at: 411 682 411
Size: 271 3850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 2176 2668 2966 1297
Size: 879 492 298 2452
Fragments arranged by size:
2452 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3825 3825
Size: 4121
BsaAI yAC'GTr
Cuts at: 1474 1474
Size: 4121
BsaBI GATnn'nnATC
Cuts at: 6 893 1635 3672 6
Size: 887 742 2037 455
Fragments arranged by size:
2037 887 742 455
BsaHI Gr'CG_yC
Cuts at: 256 482 1552 1800 2502 3998 256
Size: 226 1070 248 702 1496 379
Fragments arranged by size:
1496 1070 702 379 248 226
BsaWI w'CCGG_w
Cuts at: 1310 1831 2509 2783 2930 3341 1310
Size: 521 678 274 147 411 2090
Fragments arranged by size:
2090 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 299 625 1546 2304 2881 3377 299
Size: 326 921 758 577 496 1043
Fragments arranged by size:
1043 921 758 577 496 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 409
Size: 4121
BsiEI CG_ry'CG
Cuts at: 104 1663 1709 2917 104
Size: 1559 46 1208 1308
Fragments arranged by size:
1559 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 166 752 839 1135 1913 2103 2895 166
Size: 586 87 296 778 190 792 1392
Fragments arranged by size:
1392 792 778 586 296 190 87
BsmI GAATG_Cn'
Cuts at: 543 543
Size: 4121
Bsp1286I G_dGCh'C
Cuts at: 166 399 604 625 752 839 872 1032 1135
Size: 233 205 21 127 87 33 160 103
Cuts at: 1135 1746 1839 1913 2103 2165 2895 166
Size: 611 93 74 190 62 730 1392
Fragments arranged by size:
1392 730 611 233 205 190 160 127
103 93 87 74 62 33 21
BspEI T'CCGG_A
Cuts at: 3341 3341
Size: 4121
BspHI T'CATG_A
Cuts at: 713 3297 713
Size: 2584 1537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 590 680 1171 1385 1687 2068 590
Size: 90 491 214 302 381 2643
Fragments arranged by size:
2643 491 381 302 214 90
BsrBI CCG'CTC
Cuts at: 50 210 2413 2467 3951 4116 50
Size: 160 2203 54 1484 165 55
Fragments arranged by size:
2203 1484 165 160 55 54
BsrDI GCAATG_nn'
Cuts at: 2033 2033
Size: 4121
BsrFI r'CCGG_y
Cuts at: 265 279 2119 2300 2509 265
Size: 14 1840 181 209 1877
Fragments arranged by size:
1877 1840 209 181 14
BsrGI T'GTAC_A
Cuts at: 182 946 182
Size: 764 3357
BssSI C'ACGA_G
Cuts at: 2392 2750 2392
Size: 358 3763
BstAPI GCAn_nnn'nTGC
Cuts at: 423 1498 423
Size: 1075 3046
BstBI TT'CG_AA
Cuts at: 2482 2482
Size: 4121
BstEII G'GTnAC_C
Cuts at: 402 402
Size: 4121
BstYI r'GATC_y
Cuts at: 1 560 888 1146 1613 1636 1971 2217 3229
Size: 559 328 258 467 23 335 246 1012
Cuts at: 3229 3315 3327 3673 1
Size: 86 12 346 449
Fragments arranged by size:
1012 559 467 449 346 335 328 258
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 529 529
Size: 4121
BtgI C'CryG_G
Cuts at: 126 320 328 126
Size: 194 8 3919
Fragments arranged by size:
3919 194 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 522 2071 2252 2415 522
Size: 1549 181 163 2228
Fragments arranged by size:
2228 1549 181 163
BtsI GCAGTG_nn'
Cuts at: 143 381 1364 143
Size: 238 983 2900
Fragments arranged by size:
2900 983 238
DraI TTT'AAA
Cuts at: 1120 1120
Size: 4121
DraIII CAC_nnn'GTG
Cuts at: 161 161
Size: 4121
DrdI GACnn_nn'nnGTC
Cuts at: 1827 2685 1827
Size: 858 3263
EaeI y'GGCC_r
Cuts at: 198 260 331 1706 1880 2271 2298 3643 198
Size: 62 71 1375 174 391 27 1345 676
Fragments arranged by size:
1375 1345 676 391 174 71 62 27
EagI C'GGCC_G
Cuts at: 1706 1706
Size: 4121
EarI CTCTTCn'nnn_
Cuts at: 757 2144 2354 757
Size: 1387 210 2524
Fragments arranged by size:
2524 1387 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 301 373 2329 2639 2785 3931 301
Size: 72 1956 310 146 1146 491
Fragments arranged by size:
1956 1146 491 310 146 72
EcoICRI GAG'CTC
Cuts at: 750 837 1133 750
Size: 87 296 3738
Fragments arranged by size:
3738 296 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 853 887 1946 2378 3125 3393 724
Size: 129 34 1059 432 747 268 1452
Fragments arranged by size:
1452 1059 747 432 268 129 34
EcoNI CCTnn'n_nnAGG
Cuts at: 685 685
Size: 4121
EcoO109I rG'GnC_Cy
Cuts at: 315 621 622 1260 315
Size: 306 1 638 3176
Fragments arranged by size:
3176 638 306 1
EcoRI G'AATT_C
Cuts at: 1125 1125
Size: 4121
EcoRV GAT'ATC
Cuts at: 785 785
Size: 4121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 4121
FspI TGC'GCA
Cuts at: 1902 1902
Size: 4121
HaeII r_GCGC'y
Cuts at: 151 309 1555 1803 2825 3804 151
Size: 158 1246 248 1022 979 468
Fragments arranged by size:
1246 1022 979 468 248 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1963 1993 1995 2025 1963
Size: 30 2 30 4059
Fragments arranged by size:
4059 30 30 2
HincII GTy'rAC
Cuts at: 1160 1160
Size: 4121
KasI G'GCGC_C
Cuts at: 1551 1799 1551
Size: 248 3873
KpnI G_GTAC'C
Cuts at: 1141 1141
Size: 4121
MfeI C'AATT_G
Cuts at: 824 824
Size: 4121
MluI A'CGCG_T
Cuts at: 3554 3554
Size: 4121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 812 2792 2976 812
Size: 1980 184 1957
Fragments arranged by size:
1980 1957 184
MscI TGG'CCA
Cuts at: 1882 1882
Size: 4121
MslI CAynn'nnrTG
Cuts at: 157 472 920 2110 2237 157
Size: 315 448 1190 127 2041
Fragments arranged by size:
2041 1190 448 315 127
MspA1I CmG'CkG
Cuts at: 322 330 1232 1546 1906 2919 3164 322
Size: 8 902 314 360 1013 245 1279
Fragments arranged by size:
1279 1013 902 360 314 245 8
NaeI GCC'GGC
Cuts at: 281 2302 281
Size: 2021 2100
NarI GG'CG_CC
Cuts at: 1552 1800 1552
Size: 248 3873
NcoI C'CATG_G
Cuts at: 126 126
Size: 4121
NgoMIV G'CCGG_C
Cuts at: 279 2300 279
Size: 2021 2100
NspI r_CATG'y
Cuts at: 639 1174 1674 2581 639
Size: 535 500 907 2179
Fragments arranged by size:
2179 907 535 500
PciI A'CATG_T
Cuts at: 2577 2577
Size: 4121
PflMI CCAn_nnn'nTGG
Cuts at: 132 794 132
Size: 662 3459
PmeI GTTT'AAAC
Cuts at: 1120 1120
Size: 4121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 4089
PpuMI rG'GwC_Cy
Cuts at: 315 315
Size: 4121
PspOMI G'GGCC_C
Cuts at: 621 621
Size: 4121
PstI C_TGCA'G
Cuts at: 388 740 886 1168 1853 388
Size: 352 146 282 685 2656
Fragments arranged by size:
2656 685 352 282 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4121
PvuII CAG'CTG
Cuts at: 1546 1906 1546
Size: 360 3761
RsrII CG'GwC_CG
Cuts at: 2316 2316
Size: 4121
SacI G_AGCT'C
Cuts at: 752 839 1135 752
Size: 87 296 3738
Fragments arranged by size:
3738 296 87
SacII CC_GC'GG
Cuts at: 323 331 323
Size: 8 4113
SalI G'TCGA_C
Cuts at: 1158 1158
Size: 4121
SanDI GG'GwC_CC
Cuts at: 315 315
Size: 4121
SapI GCTCTTCn'nnn_
Cuts at: 757 2144 2354 757
Size: 1387 210 2524
Fragments arranged by size:
2524 1387 210
SbfI CC_TGCA'GG
Cuts at: 1168 1168
Size: 4121
ScaI AGT'ACT
Cuts at: 3638 3638
Size: 4121
SfcI C'TryA_G
Cuts at: 33 384 736 882 1164 1849 2842 3033 33
Size: 351 352 146 282 685 993 191 1121
Fragments arranged by size:
1121 993 685 352 351 282 191 146
SfoI GGC'GCC
Cuts at: 1553 1801 1553
Size: 248 3873
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
SmaI CCC'GGG
Cuts at: 312 1143 312
Size: 831 3290
SmlI C'TyrA_G
Cuts at: 1276 2191 2683 2945 1276
Size: 915 492 262 2452
Fragments arranged by size:
2452 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4121
SphI G_CATG'C
Cuts at: 1174 1674 1174
Size: 500 3621
SspI AAT'ATT
Cuts at: 1044 1044
Size: 4121
StuI AGG'CCT
Cuts at: 709 709
Size: 4121
StyI C'CwwG_G
Cuts at: 126 1255 1609 126
Size: 1129 354 2638
Fragments arranged by size:
2638 1129 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 313 1704 1837 2296 2505 313
Size: 1391 133 459 209 1929
Fragments arranged by size:
1929 1391 459 209 133
TatI w'GTAC_w
Cuts at: 182 946 3636 182
Size: 764 2690 667
Fragments arranged by size:
2690 764 667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4089
Tth111I GACn'n_nGTC
Cuts at: 1918 1918
Size: 4121
XbaI T'CTAG_A
Cuts at: 1152 1152
Size: 4121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1616 1616
Size: 4121
XmaI C'CCGG_G
Cuts at: 310 1141 310
Size: 831 3290
XmnI GAAnn'nnTTC
Cuts at: 709 709
Size: 4121
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BsaXI BseRI BsiWI
BsmBI BssHII BstXI BstZ17I ClaI CspCI FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI