Restriction map for
pF1KB3969
(Circular) (Six-base) MAPSORT of: pF1KB3969.seq Check: 7743 from: 1 to: 3689
pF1KB3969 3689 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 233 300 727 233
Size: 67 427 3195
Fragments arranged by size:
3195 427 67
Acc65I G'GTAC_C
Cuts at: 705 705
Size: 3689
AclI AA'CG_TT
Cuts at: 683 3529 683
Size: 2846 843
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 427 524 1514 1946 2693 2961 227
Size: 200 97 990 432 747 268 955
Fragments arranged by size:
990 955 747 432 268 200 97
AflIII A'CryG_T
Cuts at: 1041 2145 3122 1041
Size: 1104 977 1608
Fragments arranged by size:
1608 1104 977
AgeI A'CCGG_T
Cuts at: 2077 2077
Size: 3689
AleI CACnn'nnGTG
Cuts at: 516 516
Size: 3689
AlwNI CAG_nnn'CTG
Cuts at: 994 2561 994
Size: 1567 2122
ApaLI G'TGCA_C
Cuts at: 2459 2459
Size: 3689
ApoI r'AATT_y
Cuts at: 693 3042 3298 693
Size: 2349 256 1084
Fragments arranged by size:
2349 1084 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3689
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
AvaI C'yCGr_G
Cuts at: 709 709
Size: 3689
BamHI G'GATC_C
Cuts at: 714 714
Size: 3689
BanI G'GyrC_C
Cuts at: 705 1119 1367 1402 705
Size: 414 248 35 2992
Fragments arranged by size:
2992 414 248 35
BanII G_rGCy'C
Cuts at: 703 1733 703
Size: 1030 2659
BbeI G_GCGC'C
Cuts at: 1123 1371 1123
Size: 248 3441
BciVI GTATCCnnnnn_n'
Cuts at: 1587 2348 3689 1587
Size: 761 1341 1587
Fragments arranged by size:
1587 1341 761
BclI T'GATC_A
Cuts at: 1209 1209
Size: 3689
BglII A'GATC_T
Cuts at: 1204 3241 1204
Size: 2037 1652
BlpI GC'TnA_GC
Cuts at: 801 801
Size: 3689
Bme1580I G_kGCm'C
Cuts at: 1314 1407 2463 1314
Size: 93 1056 2540
Fragments arranged by size:
2540 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 901 982 1317 3461 901
Size: 81 335 2144 1129
Fragments arranged by size:
2144 1129 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 3689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1744 2236 2534 865
Size: 879 492 298 2020
Fragments arranged by size:
2020 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3393 3393
Size: 3689
BsaAI yAC'GTr
Cuts at: 1042 1042
Size: 3689
BsaBI GATnn'nnATC
Cuts at: 6 144 1203 3240 6
Size: 138 1059 2037 455
Fragments arranged by size:
2037 1059 455 138
BsaHI Gr'CG_yC
Cuts at: 1120 1368 2070 3566 1120
Size: 248 702 1496 1243
Fragments arranged by size:
1496 1243 702 248
BsaWI w'CCGG_w
Cuts at: 878 1399 2077 2351 2498 2909 878
Size: 521 678 274 147 411 1658
Fragments arranged by size:
1658 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 275 275
Size: 3689
BseYI C'CCAG_C
Cuts at: 1114 1872 2449 2945 1114
Size: 758 577 496 1858
Fragments arranged by size:
1858 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1231 1277 2485 104
Size: 1127 46 1208 1308
Fragments arranged by size:
1308 1208 1127 46
BsiHKAI G_wGCw'C
Cuts at: 703 1481 1671 2463 703
Size: 778 190 792 1929
Fragments arranged by size:
1929 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 703 1314 1407 1481 1671 1733 2463 703
Size: 611 93 74 190 62 730 1929
Fragments arranged by size:
1929 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2909 2909
Size: 3689
BspHI T'CATG_A
Cuts at: 2865 2865
Size: 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 739 953 1255 1636 739
Size: 214 302 381 2792
Fragments arranged by size:
2792 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1981 2035 3519 3684 50
Size: 1931 54 1484 165 55
Fragments arranged by size:
1931 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1601 1601
Size: 3689
BsrFI r'CCGG_y
Cuts at: 1687 1868 2077 1687
Size: 181 209 3299
Fragments arranged by size:
3299 209 181
BsrGI T'GTAC_A
Cuts at: 362 362
Size: 3689
BssSI C'ACGA_G
Cuts at: 1960 2318 1960
Size: 358 3331
BstAPI GCAn_nnn'nTGC
Cuts at: 1066 1066
Size: 3689
BstBI TT'CG_AA
Cuts at: 2050 2050
Size: 3689
BstYI r'GATC_y
Cuts at: 1 714 1181 1204 1539 1785 2797 2883 2895
Size: 713 467 23 335 246 1012 86 12
Cuts at: 2895 3241 1
Size: 346 449
Fragments arranged by size:
1012 713 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 234 234
Size: 3689
BtgI C'CryG_G
Cuts at: 107 107
Size: 3689
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1639 1820 1983 1639
Size: 181 163 3345
Fragments arranged by size:
3345 181 163
BtsI GCAGTG_nn'
Cuts at: 523 932 523
Size: 409 3280
ClaI AT'CG_AT
Cuts at: 419 419
Size: 3689
DraI TTT'AAA
Cuts at: 688 688
Size: 3689
DrdI GACnn_nn'nnGTC
Cuts at: 1395 2253 1395
Size: 858 2831
EaeI y'GGCC_r
Cuts at: 1274 1448 1839 1866 3211 1274
Size: 174 391 27 1345 1752
Fragments arranged by size:
1752 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1274 1274
Size: 3689
EarI CTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1897 2207 2353 3499 1897
Size: 310 146 1146 2087
Fragments arranged by size:
2087 1146 310 146
EcoICRI GAG'CTC
Cuts at: 701 701
Size: 3689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 427 524 1514 1946 2693 2961 227
Size: 200 97 990 432 747 268 955
Fragments arranged by size:
990 955 747 432 268 200 97
EcoO109I rG'GnC_Cy
Cuts at: 828 828
Size: 3689
EcoRI G'AATT_C
Cuts at: 693 693
Size: 3689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 656 1708 656
Size: 1052 2637
FspI TGC'GCA
Cuts at: 1470 1470
Size: 3689
HaeII r_GCGC'y
Cuts at: 1123 1371 2393 3372 1123
Size: 248 1022 979 1440
Fragments arranged by size:
1440 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1531 1561 1563 1593 1531
Size: 30 2 30 3627
Fragments arranged by size:
3627 30 30 2
HincII GTy'rAC
Cuts at: 728 728
Size: 3689
HindIII A'AGCT_T
Cuts at: 633 633
Size: 3689
KasI G'GCGC_C
Cuts at: 1119 1367 1119
Size: 248 3441
KpnI G_GTAC'C
Cuts at: 709 709
Size: 3689
MfeI C'AATT_G
Cuts at: 449 449
Size: 3689
MluI A'CGCG_T
Cuts at: 3122 3122
Size: 3689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2544 2360
Size: 184 3505
MscI TGG'CCA
Cuts at: 1450 1450
Size: 3689
MslI CAynn'nnrTG
Cuts at: 284 516 1678 1805 284
Size: 232 1162 127 2168
Fragments arranged by size:
2168 1162 232 127
MspA1I CmG'CkG
Cuts at: 800 1114 1474 2487 2732 800
Size: 314 360 1013 245 1757
Fragments arranged by size:
1757 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1870 1870
Size: 3689
NarI GG'CG_CC
Cuts at: 1120 1368 1120
Size: 248 3441
NcoI C'CATG_G
Cuts at: 107 107
Size: 3689
NgoMIV G'CCGG_C
Cuts at: 1868 1868
Size: 3689
NspI r_CATG'y
Cuts at: 742 1242 2149 742
Size: 500 907 2282
Fragments arranged by size:
2282 907 500
PciI A'CATG_T
Cuts at: 2145 2145
Size: 3689
PmeI GTTT'AAAC
Cuts at: 688 688
Size: 3689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 3657
PstI C_TGCA'G
Cuts at: 736 1421 736
Size: 685 3004
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3689
PvuII CAG'CTG
Cuts at: 1114 1474 1114
Size: 360 3329
RsrII CG'GwC_CG
Cuts at: 1884 1884
Size: 3689
SacI G_AGCT'C
Cuts at: 703 703
Size: 3689
SalI G'TCGA_C
Cuts at: 726 726
Size: 3689
SapI GCTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
SbfI CC_TGCA'GG
Cuts at: 736 736
Size: 3689
ScaI AGT'ACT
Cuts at: 3206 3206
Size: 3689
SfcI C'TryA_G
Cuts at: 33 301 732 1417 2410 2601 33
Size: 268 431 685 993 191 1121
Fragments arranged by size:
1121 993 685 431 268 191
SfoI GGC'GCC
Cuts at: 1121 1369 1121
Size: 248 3441
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
SmaI CCC'GGG
Cuts at: 711 711
Size: 3689
SmlI C'TyrA_G
Cuts at: 844 1759 2251 2513 844
Size: 915 492 262 2020
Fragments arranged by size:
2020 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3689
SphI G_CATG'C
Cuts at: 742 1242 742
Size: 500 3189
StyI C'CwwG_G
Cuts at: 107 823 1177 107
Size: 716 354 2619
Fragments arranged by size:
2619 716 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 272 1272 1405 1864 2073 272
Size: 1000 133 459 209 1888
Fragments arranged by size:
1888 1000 459 209 133
TatI w'GTAC_w
Cuts at: 362 3204 362
Size: 2842 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 3657
Tth111I GACn'n_nGTC
Cuts at: 1486 1486
Size: 3689
XbaI T'CTAG_A
Cuts at: 720 720
Size: 3689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1184 1184
Size: 3689
XmaI C'CCGG_G
Cuts at: 709 709
Size: 3689
XmnI GAAnn'nnTTC
Cuts at: 386 386
Size: 3689
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstXI Bsu36I CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI