Restriction map for
pF1KB3968
(Circular) (Six-base) MAPSORT of: pF1KB3968.seq Check: 9619 from: 1 to: 4448
pF1KB3968 4448 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 455 1292 1486 455
Size: 837 194 3417
Fragments arranged by size:
3417 837 194
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 2705 3452 3720 2273
Size: 432 747 268 3001
Fragments arranged by size:
3001 747 432 268
AflIII A'CryG_T
Cuts at: 692 1800 2904 3881 692
Size: 1108 1104 977 1259
Fragments arranged by size:
1259 1108 1104 977
AgeI A'CCGG_T
Cuts at: 605 2836 605
Size: 2231 2217
AhdI GACnn_n'nnGTC
Cuts at: 165 165
Size: 4448
AlwNI CAG_nnn'CTG
Cuts at: 247 1269 1347 1753 3320 247
Size: 1022 78 406 1567 1375
Fragments arranged by size:
1567 1375 1022 406 78
ApaI G_GGCC'C
Cuts at: 282 282
Size: 4448
ApaLI G'TGCA_C
Cuts at: 3218 3218
Size: 4448
ApoI r'AATT_y
Cuts at: 1452 3801 4057 1452
Size: 2349 256 1843
Fragments arranged by size:
2349 1843 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 154 1468 154
Size: 1314 3134
BamHI G'GATC_C
Cuts at: 1473 1473
Size: 4448
BanI G'GyrC_C
Cuts at: 1464 1878 2126 2161 1464
Size: 414 248 35 3751
Fragments arranged by size:
3751 414 248 35
BanII G_rGCy'C
Cuts at: 282 1025 1462 2492 282
Size: 743 437 1030 2238
Fragments arranged by size:
2238 1030 743 437
BbeI G_GCGC'C
Cuts at: 1882 2130 1882
Size: 248 4200
BbsI GAAGACnn'nnnn_
Cuts at: 287 886 287
Size: 599 3849
BbvCI CC'TCA_GC
Cuts at: 118 118
Size: 4448
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3107 4448 2346
Size: 761 1341 2346
Fragments arranged by size:
2346 1341 761
BclI T'GATC_A
Cuts at: 1968 1968
Size: 4448
BglI GCCn_nnn'nGGC
Cuts at: 1238 1238
Size: 4448
BglII A'GATC_T
Cuts at: 1963 4000 1963
Size: 2037 2411
BlpI GC'TnA_GC
Cuts at: 490 1560 490
Size: 1070 3378
Bme1580I G_kGCm'C
Cuts at: 282 2073 2166 3222 282
Size: 1791 93 1056 1508
Fragments arranged by size:
1791 1508 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1660 1741 2076 4220 1660
Size: 81 335 2144 1888
Fragments arranged by size:
2144 1888 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4448
Bpu10I CC'TnA_GC
Cuts at: 118 118
Size: 4448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 2503 2995 3293 1624
Size: 879 492 298 2779
Fragments arranged by size:
2779 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1400 4152 1400
Size: 2752 1696
BsaAI yAC'GTr
Cuts at: 1801 1801
Size: 4448
BsaBI GATnn'nnATC
Cuts at: 6 394 1962 3999 6
Size: 388 1568 2037 455
Fragments arranged by size:
2037 1568 455 388
BsaHI Gr'CG_yC
Cuts at: 1879 2127 2829 4325 1879
Size: 248 702 1496 2002
Fragments arranged by size:
2002 1496 702 248
BsaWI w'CCGG_w
Cuts at: 605 1097 1637 2158 2836 3110 3257 3668 605
Size: 492 540 521 678 274 147 411 1385
Fragments arranged by size:
1385 678 540 521 492 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 157 187 157
Size: 30 4418
BseYI C'CCAG_C
Cuts at: 1873 2631 3208 3704 1873
Size: 758 577 496 2617
Fragments arranged by size:
2617 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1990 2036 3244 104
Size: 1886 46 1208 1308
Fragments arranged by size:
1886 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1462 2240 2430 3222 1462
Size: 778 190 792 2688
Fragments arranged by size:
2688 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 282 1025 1462 2073 2166 2240 2430 2492 3222
Size: 743 437 611 93 74 190 62 730
Cuts at: 3222 282
Size: 1508
Fragments arranged by size:
1508 743 730 611 437 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1097 3668 1097
Size: 2571 1877
BspHI T'CATG_A
Cuts at: 3624 3624
Size: 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1498 1712 2014 2395 1498
Size: 214 302 381 3551
Fragments arranged by size:
3551 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2740 2794 4278 4443 50
Size: 2690 54 1484 165 55
Fragments arranged by size:
2690 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 715 2360 715
Size: 1645 2803
BsrFI r'CCGG_y
Cuts at: 141 605 2446 2627 2836 141
Size: 464 1841 181 209 1753
Fragments arranged by size:
1841 1753 464 209 181
BssSI C'ACGA_G
Cuts at: 2719 3077 2719
Size: 358 4090
BstAPI GCAn_nnn'nTGC
Cuts at: 1825 1825
Size: 4448
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstYI r'GATC_y
Cuts at: 1 1473 1940 1963 2298 2544 3556 3642 3654
Size: 1472 467 23 335 246 1012 86 12
Cuts at: 3654 4000 1
Size: 346 449
Fragments arranged by size:
1472 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1293 1293
Size: 4448
BtgI C'CryG_G
Cuts at: 1233 1233
Size: 4448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1110 1281 2398 2579 2742 1110
Size: 171 1117 181 163 2816
Fragments arranged by size:
2816 1117 181 171 163
BtsI GCAGTG_nn'
Cuts at: 1691 1691
Size: 4448
DraI TTT'AAA
Cuts at: 1200 1447 1200
Size: 247 4201
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3012 2154
Size: 858 3590
EaeI y'GGCC_r
Cuts at: 338 1230 1274 1357 2033 2207 2598 2625 3970
Size: 892 44 83 676 174 391 27 1345
Cuts at: 3970 338
Size: 816
Fragments arranged by size:
1345 892 816 676 391 174 83 44
27
EagI C'GGCC_G
Cuts at: 2033 2033
Size: 4448
EarI CTCTTCn'nnn_
Cuts at: 569 2471 2681 569
Size: 1902 210 2336
Fragments arranged by size:
2336 1902 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2656 2966 3112 4258 2656
Size: 310 146 1146 2846
Fragments arranged by size:
2846 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1460 1460
Size: 4448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 2705 3452 3720 2273
Size: 432 747 268 3001
Fragments arranged by size:
3001 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 480 480
Size: 4448
EcoO109I rG'GnC_Cy
Cuts at: 267 278 279 1315 1587 267
Size: 11 1 1036 272 3128
Fragments arranged by size:
3128 1036 272 11 1
EcoRI G'AATT_C
Cuts at: 1452 1452
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 2229 2229
Size: 4448
HaeII r_GCGC'y
Cuts at: 1882 2130 3152 4131 1882
Size: 248 1022 979 2199
Fragments arranged by size:
2199 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 157 183 187 189 219 953 985 2290
Size: 6 26 4 2 30 734 32 1305
Cuts at: 2290 2320 2322 2352 151
Size: 30 2 30 2247
Fragments arranged by size:
2247 1305 734 32 30 30 30 26
6 4 2 2
HincII GTy'rAC
Cuts at: 1487 1487
Size: 4448
KasI G'GCGC_C
Cuts at: 1878 2126 1878
Size: 248 4200
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 829 3119 3303 311
Size: 518 2290 184 1456
Fragments arranged by size:
2290 1456 518 184
MscI TGG'CCA
Cuts at: 340 1232 1276 2209 340
Size: 892 44 933 2579
Fragments arranged by size:
2579 933 892 44
MslI CAynn'nnrTG
Cuts at: 464 2437 2564 464
Size: 1973 127 2348
Fragments arranged by size:
2348 1973 127
MspA1I CmG'CkG
Cuts at: 494 1559 1873 2233 3246 3491 494
Size: 1065 314 360 1013 245 1451
Fragments arranged by size:
1451 1065 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2629 2629
Size: 4448
NarI GG'CG_CC
Cuts at: 1879 2127 1879
Size: 248 4200
NcoI C'CATG_G
Cuts at: 1233 1233
Size: 4448
NdeI CA'TA_TG
Cuts at: 465 465
Size: 4448
NgoMIV G'CCGG_C
Cuts at: 2627 2627
Size: 4448
NspI r_CATG'y
Cuts at: 648 696 1501 2001 2908 648
Size: 48 805 500 907 2188
Fragments arranged by size:
2188 907 805 500 48
PciI A'CATG_T
Cuts at: 692 2904 692
Size: 2212 2236
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4416
PpuMI rG'GwC_Cy
Cuts at: 267 267
Size: 4448
PspOMI G'GGCC_C
Cuts at: 278 278
Size: 4448
PstI C_TGCA'G
Cuts at: 252 686 1046 1495 2180 252
Size: 434 360 449 685 2520
Fragments arranged by size:
2520 685 449 434 360
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 494 1873 2233 494
Size: 1379 360 2709
Fragments arranged by size:
2709 1379 360
RsrII CG'GwC_CG
Cuts at: 1095 2643 1095
Size: 1548 2900
SacI G_AGCT'C
Cuts at: 1462 1462
Size: 4448
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 569 2471 2681 569
Size: 1902 210 2336
Fragments arranged by size:
2336 1902 210
SbfI CC_TGCA'GG
Cuts at: 1046 1495 1046
Size: 449 3999
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SfcI C'TryA_G
Cuts at: 33 248 682 1042 1491 2176 3169 3360 33
Size: 215 434 360 449 685 993 191 1121
Fragments arranged by size:
1121 993 685 449 434 360 215 191
SfoI GGC'GCC
Cuts at: 1880 2128 1880
Size: 248 4200
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 1470 1470
Size: 4448
SmlI C'TyrA_G
Cuts at: 1603 2518 3010 3272 1603
Size: 915 492 262 2779
Fragments arranged by size:
2779 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1501 2001 1501
Size: 500 3948
StyI C'CwwG_G
Cuts at: 1233 1582 1936 1233
Size: 349 354 3745
Fragments arranged by size:
3745 354 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 306 2031 2164 2623 2832 306
Size: 1725 133 459 209 1922
Fragments arranged by size:
1922 1725 459 209 133
TatI w'GTAC_w
Cuts at: 651 983 1049 3963 651
Size: 332 66 2914 1136
Fragments arranged by size:
2914 1136 332 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 4416
Tth111I GACn'n_nGTC
Cuts at: 2245 2245
Size: 4448
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1078 1943 1078
Size: 865 3583
XmaI C'CCGG_G
Cuts at: 1468 1468
Size: 4448
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BpmI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI