Restriction map for
pF1KB3938
(Circular) (Six-base) MAPSORT of: pF1KB3938.seq Check: 3504 from: 1 to: 4439
pF1KB3938 4439 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 318 318
Size: 4439
AccI GT'mk_AC
Cuts at: 584 1477 584
Size: 893 3546
Acc65I G'GTAC_C
Cuts at: 1455 1455
Size: 4439
AclI AA'CG_TT
Cuts at: 4279 4279
Size: 4439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 1162 2264 2696 3049 3711 833
Size: 329 1102 432 353 662 1561
Fragments arranged by size:
1561 1102 662 432 353 329
AflIII A'CryG_T
Cuts at: 1791 3591 3872 1791
Size: 1800 281 2358
Fragments arranged by size:
2358 1800 281
AleI CACnn'nnGTG
Cuts at: 424 424
Size: 4439
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 731 763 731
Size: 32 4407
AlwNI CAG_nnn'CTG
Cuts at: 1744 3182 1744
Size: 1438 3001
ApaLI G'TGCA_C
Cuts at: 3277 3277
Size: 4439
ApoI r'AATT_y
Cuts at: 1239 1443 3792 4048 1239
Size: 204 2349 256 1630
Fragments arranged by size:
2349 1630 256 204
AseI AT'TA_AT
Cuts at: 20 20
Size: 4439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
AvaI C'yCGr_G
Cuts at: 1459 1459
Size: 4439
BamHI G'GATC_C
Cuts at: 1464 1464
Size: 4439
BanI G'GyrC_C
Cuts at: 306 318 636 1319 1455 1869 2117 2152 306
Size: 12 318 683 136 414 248 35 2593
Fragments arranged by size:
2593 683 414 318 248 136 35 12
BanII G_rGCy'C
Cuts at: 1453 2483 1453
Size: 1030 3409
BbeI G_GCGC'C
Cuts at: 1873 2121 1873
Size: 248 4191
BbsI GAAGACnn'nnnn_
Cuts at: 898 898
Size: 4439
BbvCI CC'TCA_GC
Cuts at: 1129 1129
Size: 4439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4405
BciVI GTATCCnnnnn_n'
Cuts at: 2337 3393 4439 2337
Size: 1056 1046 2337
Fragments arranged by size:
2337 1056 1046
BclI T'GATC_A
Cuts at: 1044 1386 1959 1044
Size: 342 573 3524
Fragments arranged by size:
3524 573 342
BglI GCCn_nnn'nGGC
Cuts at: 292 535 292
Size: 243 4196
BglII A'GATC_T
Cuts at: 1954 3991 1954
Size: 2037 2402
BlpI GC'TnA_GC
Cuts at: 1551 1551
Size: 4439
Bme1580I G_kGCm'C
Cuts at: 2064 2157 3281 2064
Size: 93 1124 3222
Fragments arranged by size:
3222 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1651 1732 2067 4211 1651
Size: 81 335 2144 1879
Fragments arranged by size:
2144 1879 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4439
Bpu10I CC'TnA_GC
Cuts at: 1129 1129
Size: 4439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 2494 3208 3506 1615
Size: 879 714 298 2548
Fragments arranged by size:
2548 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 539 795 4143 539
Size: 256 3348 835
Fragments arranged by size:
3348 835 256
BsaAI yAC'GTr
Cuts at: 1792 1792
Size: 4439
BsaBI GATnn'nnATC
Cuts at: 6 1953 3990 6
Size: 1947 2037 455
Fragments arranged by size:
2037 1947 455
BsaHI Gr'CG_yC
Cuts at: 247 1870 2118 2820 4316 247
Size: 1623 248 702 1496 370
Fragments arranged by size:
1623 1496 702 370 248
BsaWI w'CCGG_w
Cuts at: 1628 2149 2827 3238 3385 3659 1628
Size: 521 678 411 147 274 2408
Fragments arranged by size:
2408 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 202
Size: 4439
BseYI C'CCAG_C
Cuts at: 447 1864 2622 3287 3695 447
Size: 1417 758 665 408 1191
Fragments arranged by size:
1417 1191 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 538
Size: 4439
BsiEI CG_ry'CG
Cuts at: 104 301 1981 2027 3257 104
Size: 197 1680 46 1230 1286
Fragments arranged by size:
1680 1286 1230 197 46
BsiHKAI G_wGCw'C
Cuts at: 690 1453 2231 2421 3281 690
Size: 763 778 190 860 1848
Fragments arranged by size:
1848 860 778 763 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1053 1053
Size: 4439
Bsp1286I G_dGCh'C
Cuts at: 690 1453 2064 2157 2231 2421 2483 3281 690
Size: 763 611 93 74 190 62 798 1848
Fragments arranged by size:
1848 798 763 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2871 2871
Size: 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 318 557 1489 1703 2005 2386 318
Size: 239 932 214 302 381 2371
Fragments arranged by size:
2371 932 381 302 239 214
BsrBI CCG'CTC
Cuts at: 50 2731 2785 4269 4434 50
Size: 2681 54 1484 165 55
Fragments arranged by size:
2681 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2351 2351
Size: 4439
BsrFI r'CCGG_y
Cuts at: 2437 2618 2437
Size: 181 4258
BssSI C'ACGA_G
Cuts at: 2710 3418 2710
Size: 708 3731
BstAPI GCAn_nnn'nTGC
Cuts at: 1816 1816
Size: 4439
BstBI TT'CG_AA
Cuts at: 2800 2800
Size: 4439
BstYI r'GATC_y
Cuts at: 1 176 343 1464 1931 1954 2289 2535 2841
Size: 175 167 1121 467 23 335 246 306
Cuts at: 2841 2853 2939 3991 1
Size: 12 86 1052 449
Fragments arranged by size:
1121 1052 467 449 335 306 246 175
167 86 23 12
BtgI C'CryG_G
Cuts at: 659 659
Size: 4439
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2389 2570 2733 2389
Size: 181 163 4095
Fragments arranged by size:
4095 181 163
BtsI GCAGTG_nn'
Cuts at: 971 1682 971
Size: 711 3728
DraI TTT'AAA
Cuts at: 1040 1438 1040
Size: 398 4041
DrdI GACnn_nn'nnGTC
Cuts at: 2145 3489 2145
Size: 1344 3095
EaeI y'GGCC_r
Cuts at: 607 965 1070 2024 2198 2589 2616 3961 607
Size: 358 105 954 174 391 27 1345 1085
Fragments arranged by size:
1345 1085 954 391 358 174 105 27
EagI C'GGCC_G
Cuts at: 2024 2024
Size: 4439
EarI CTCTTCn'nnn_
Cuts at: 2462 2672 2462
Size: 210 4229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 338 2647 3389 3535 4249 338
Size: 2309 742 146 714 528
Fragments arranged by size:
2309 742 714 528 146
EcoICRI GAG'CTC
Cuts at: 1451 1451
Size: 4439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 1162 2264 2696 3049 3711 833
Size: 329 1102 432 353 662 1561
Fragments arranged by size:
1561 1102 662 432 353 329
EcoO109I rG'GnC_Cy
Cuts at: 382 1578 382
Size: 1196 3243
EcoRI G'AATT_C
Cuts at: 1239 1443 1239
Size: 204 4235
EcoRV GAT'ATC
Cuts at: 706 706
Size: 4439
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1323 2458 1323
Size: 1135 3304
FspI TGC'GCA
Cuts at: 2220 2220
Size: 4439
HaeII r_GCGC'y
Cuts at: 1873 2121 3351 4122 1873
Size: 248 1230 771 2190
Fragments arranged by size:
2190 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2281 2311 2313 2343 2281
Size: 30 2 30 4377
Fragments arranged by size:
4377 30 30 2
HincII GTy'rAC
Cuts at: 1478 1478
Size: 4439
KasI G'GCGC_C
Cuts at: 1869 2117 1869
Size: 248 4191
KpnI G_GTAC'C
Cuts at: 1459 1459
Size: 4439
MfeI C'AATT_G
Cuts at: 1256 1256
Size: 4439
MluI A'CGCG_T
Cuts at: 3872 3872
Size: 4439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 305 3198 3382 305
Size: 2893 184 1362
Fragments arranged by size:
2893 1362 184
MscI TGG'CCA
Cuts at: 609 967 1072 2200 609
Size: 358 105 1128 2848
Fragments arranged by size:
2848 1128 358 105
MslI CAynn'nnrTG
Cuts at: 424 1308 1394 2428 2555 424
Size: 884 86 1034 127 2308
Fragments arranged by size:
2308 1034 884 127 86
MspA1I CmG'CkG
Cuts at: 1550 1864 2224 3008 3253 1550
Size: 314 360 784 245 2736
Fragments arranged by size:
2736 784 360 314 245
NaeI GCC'GGC
Cuts at: 2620 2620
Size: 4439
NarI GG'CG_CC
Cuts at: 1870 2118 1870
Size: 248 4191
NgoMIV G'CCGG_C
Cuts at: 2618 2618
Size: 4439
NspI r_CATG'y
Cuts at: 504 1029 1492 1992 3595 504
Size: 525 463 500 1603 1348
Fragments arranged by size:
1603 1348 525 500 463
PciI A'CATG_T
Cuts at: 3591 3591
Size: 4439
PmeI GTTT'AAAC
Cuts at: 1438 1438
Size: 4439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 4407
PpuMI rG'GwC_Cy
Cuts at: 382 382
Size: 4439
PsiI TTA'TAA
Cuts at: 1157 1157
Size: 4439
PstI C_TGCA'G
Cuts at: 1486 2171 1486
Size: 685 3754
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4439
PvuII CAG'CTG
Cuts at: 1864 2224 1864
Size: 360 4079
RsrII CG'GwC_CG
Cuts at: 2634 2634
Size: 4439
SacI G_AGCT'C
Cuts at: 1453 1453
Size: 4439
SalI G'TCGA_C
Cuts at: 1476 1476
Size: 4439
SapI GCTCTTCn'nnn_
Cuts at: 2462 2672 2462
Size: 210 4229
SbfI CC_TGCA'GG
Cuts at: 1486 1486
Size: 4439
ScaI AGT'ACT
Cuts at: 3956 3956
Size: 4439
SfcI C'TryA_G
Cuts at: 33 1482 2167 3135 3326 33
Size: 1449 685 968 191 1146
Fragments arranged by size:
1449 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1871 2119 1871
Size: 248 4191
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
SmaI CCC'GGG
Cuts at: 1461 1461
Size: 4439
SmlI C'TyrA_G
Cuts at: 1594 2509 3223 3485 1594
Size: 915 714 262 2548
Fragments arranged by size:
2548 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4439
SphI G_CATG'C
Cuts at: 504 1029 1492 1992 504
Size: 525 463 500 2951
Fragments arranged by size:
2951 525 500 463
StyI C'CwwG_G
Cuts at: 272 378 1346 1573 1927 272
Size: 106 968 227 354 2784
Fragments arranged by size:
2784 968 354 227 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 267 774 2022 2155 2614 2823 267
Size: 507 1248 133 459 209 1883
Fragments arranged by size:
1883 1248 507 459 209 133
TatI w'GTAC_w
Cuts at: 3954 3954
Size: 4439
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 656 688 3956 3988 656
Size: 32 3268 32 1107
Fragments arranged by size:
3268 1107 32 32
Tth111I GACn'n_nGTC
Cuts at: 2236 2236
Size: 4439
XbaI T'CTAG_A
Cuts at: 1470 1470
Size: 4439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 288 537 1934 288
Size: 249 1397 2793
Fragments arranged by size:
2793 1397 249
XmaI C'CCGG_G
Cuts at: 1459 1459
Size: 4439
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BpmI BsaXI BsiWI
BsmI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI