Restriction map for
pF1KB3919
(Circular) (Six-base) MAPSORT of: pF1KB3919.seq Check: 875 from: 1 to: 4850
pF1KB3919 4850 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 978 978
Size: 4850
AccI GT'mk_AC
Cuts at: 1888 1888
Size: 4850
Acc65I G'GTAC_C
Cuts at: 1866 1866
Size: 4850
AclI AA'CG_TT
Cuts at: 4690 4690
Size: 4850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 878 1229 1262 1630 2675 3107 3854 4122
Size: 538 351 33 368 1045 432 747 268
Cuts at: 4122 340
Size: 1068
Fragments arranged by size:
1068 1045 747 538 432 368 351 268
33
AflIII A'CryG_T
Cuts at: 224 1111 2202 3306 4283 224
Size: 887 1091 1104 977 791
Fragments arranged by size:
1104 1091 977 887 791
AgeI A'CCGG_T
Cuts at: 3238 3238
Size: 4850
AlwNI CAG_nnn'CTG
Cuts at: 2155 3722 2155
Size: 1567 3283
ApaI G_GGCC'C
Cuts at: 258 1274 258
Size: 1016 3834
ApaLI G'TGCA_C
Cuts at: 3620 3620
Size: 4850
ApoI r'AATT_y
Cuts at: 543 922 1514 1650 1763 1772 1854 4203 4459
Size: 379 592 136 113 9 82 2349 256
Cuts at: 4459 543
Size: 934
Fragments arranged by size:
2349 934 592 379 256 136 113 82
9
AseI AT'TA_AT
Cuts at: 20 20
Size: 4850
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
AvaI C'yCGr_G
Cuts at: 611 1870 611
Size: 1259 3591
BamHI G'GATC_C
Cuts at: 664 1875 664
Size: 1211 3639
BanI G'GyrC_C
Cuts at: 751 862 1360 1866 2280 2528 2563 751
Size: 111 498 506 414 248 35 3038
Fragments arranged by size:
3038 506 498 414 248 111 35
BanII G_rGCy'C
Cuts at: 258 643 1274 1864 2894 258
Size: 385 631 590 1030 2214
Fragments arranged by size:
2214 1030 631 590 385
BbeI G_GCGC'C
Cuts at: 2284 2532 2284
Size: 248 4602
BbsI GAAGACnn'nnnn_
Cuts at: 220 220
Size: 4850
BbvCI CC'TCA_GC
Cuts at: 133 1124 133
Size: 991 3859
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 328 362 1384 1418 328
Size: 34 1022 34 3760
Fragments arranged by size:
3760 1022 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 681 2748 3509 4850 681
Size: 2067 761 1341 681
Fragments arranged by size:
2067 1341 761 681
BclI T'GATC_A
Cuts at: 2370 2370
Size: 4850
BfrBI ATG'CAT
Cuts at: 900 900
Size: 4850
BglI GCCn_nnn'nGGC
Cuts at: 262 271 262
Size: 9 4841
BglII A'GATC_T
Cuts at: 2365 4402 2365
Size: 2037 2813
BlpI GC'TnA_GC
Cuts at: 1962 1962
Size: 4850
Bme1580I G_kGCm'C
Cuts at: 258 1274 2475 2568 3624 258
Size: 1016 1201 93 1056 1484
Fragments arranged by size:
1484 1201 1056 1016 93
BmrI ACTGGGnnnn_n'
Cuts at: 777 2062 2143 2478 4622 777
Size: 1285 81 335 2144 1005
Fragments arranged by size:
2144 1285 1005 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3217 3217
Size: 4850
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 644 910 1418 1433 644
Size: 266 508 15 4061
Fragments arranged by size:
4061 508 266 15
Bpu10I CC'TnA_GC
Cuts at: 133 1124 1505 133
Size: 991 381 3478
Fragments arranged by size:
3478 991 381
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 630 896 2026 2905 3397 3695 244
Size: 386 266 1130 879 492 298 1399
Fragments arranged by size:
1399 1130 879 492 386 298 266
BsaI GGTCTCn'nnnn_
Cuts at: 662 4554 662
Size: 3892 958
BsaAI yAC'GTr
Cuts at: 2203 2203
Size: 4850
BsaBI GATnn'nnATC
Cuts at: 6 669 841 2364 4401 6
Size: 663 172 1523 2037 455
Fragments arranged by size:
2037 1523 663 455 172
BsaHI Gr'CG_yC
Cuts at: 179 975 2281 2529 3231 4727 179
Size: 796 1306 248 702 1496 302
Fragments arranged by size:
1496 1306 796 702 302 248
BsaWI w'CCGG_w
Cuts at: 2039 2560 3238 3512 3659 4070 2039
Size: 521 678 274 147 411 2819
Fragments arranged by size:
2819 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1029 1059 1029
Size: 30 4820
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 633 1141 1144 1280 633
Size: 508 3 136 4203
Fragments arranged by size:
4203 508 136 3
BseYI C'CCAG_C
Cuts at: 251 587 1344 2275 3033 3610 4106 251
Size: 336 757 931 758 577 496 995
Fragments arranged by size:
995 931 758 757 577 496 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 1682 280
Size: 1402 3448
BsiEI CG_ry'CG
Cuts at: 104 2392 2438 3646 104
Size: 2288 46 1208 1308
Fragments arranged by size:
2288 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 323 1864 2642 2832 3624 323
Size: 1541 778 190 792 1549
Fragments arranged by size:
1549 1541 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 258 323 643 1274 1864 2475 2568 2642 2832
Size: 65 320 631 590 611 93 74 190
Cuts at: 2832 2894 3624 258
Size: 62 730 1484
Fragments arranged by size:
1484 730 631 611 590 320 190 93
74 65 62
BspEI T'CCGG_A
Cuts at: 4070 4070
Size: 4850
BspHI T'CATG_A
Cuts at: 1136 4026 1136
Size: 2890 1960
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1900 2114 2416 2797 1900
Size: 214 302 381 3953
Fragments arranged by size:
3953 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3142 3196 4680 4845 50
Size: 3092 54 1484 165 55
Fragments arranged by size:
3092 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1048 1107 2762 1048
Size: 59 1655 3136
Fragments arranged by size:
3136 1655 59
BsrFI r'CCGG_y
Cuts at: 2848 3029 3238 2848
Size: 181 209 4460
Fragments arranged by size:
4460 209 181
BssSI C'ACGA_G
Cuts at: 1679 3121 3479 1679
Size: 1442 358 3050
Fragments arranged by size:
3050 1442 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1450 2227 1450
Size: 777 4073
BstBI TT'CG_AA
Cuts at: 3211 3211
Size: 4850
BstXI CCAn_nnnn'nTGG
Cuts at: 727 727
Size: 4850
BstYI r'GATC_y
Cuts at: 1 185 664 935 1875 2342 2365 2700 2946
Size: 184 479 271 940 467 23 335 246
Cuts at: 2946 3958 4044 4056 4402 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 940 479 467 449 346 335 271
246 184 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1266 1266
Size: 4850
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2800 2981 3144 2800
Size: 181 163 4506
Fragments arranged by size:
4506 181 163
BtsI GCAGTG_nn'
Cuts at: 2093 2093
Size: 4850
ClaI AT'CG_AT
Cuts at: 794 794
Size: 4850
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 705 740 705
Size: 35 4815
DraI TTT'AAA
Cuts at: 1849 1849
Size: 4850
DrdI GACnn_nn'nnGTC
Cuts at: 2556 3414 2556
Size: 858 3992
EaeI y'GGCC_r
Cuts at: 581 2435 2609 3000 3027 4372 581
Size: 1854 174 391 27 1345 1059
Fragments arranged by size:
1854 1345 1059 391 174 27
EagI C'GGCC_G
Cuts at: 2435 2435
Size: 4850
EarI CTCTTCn'nnn_
Cuts at: 926 1553 2873 3083 926
Size: 627 1320 210 2693
Fragments arranged by size:
2693 1320 627 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3058 3368 3514 4660 3058
Size: 310 146 1146 3248
Fragments arranged by size:
3248 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1862 1862
Size: 4850
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 644 878 910 1229 1262 1418 1433 1630
Size: 304 234 32 319 33 156 15 197
Cuts at: 1630 2675 3107 3854 4122 340
Size: 1045 432 747 268 1068
Fragments arranged by size:
1068 1045 747 432 319 304 268 234
197 156 33 32 15
EcoO109I rG'GnC_Cy
Cuts at: 199 255 1270 1271 1989 199
Size: 56 1015 1 718 3060
Fragments arranged by size:
3060 1015 718 56 1
EcoRI G'AATT_C
Cuts at: 1854 1854
Size: 4850
EcoRV GAT'ATC
Cuts at: 1043 1043
Size: 4850
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 4850
FspI TGC'GCA
Cuts at: 2631 2631
Size: 4850
HaeII r_GCGC'y
Cuts at: 277 2284 2532 3554 4533 277
Size: 2007 248 1022 979 594
Fragments arranged by size:
2007 1022 979 594 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 939 971 1242 1274 1317 1349 2692 2722 2724
Size: 32 271 32 43 32 1343 30 2
Cuts at: 2724 2754 939
Size: 30 3035
Fragments arranged by size:
3035 1343 271 43 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1889 1889
Size: 4850
KasI G'GCGC_C
Cuts at: 2280 2528 2280
Size: 248 4602
KpnI G_GTAC'C
Cuts at: 1870 1870
Size: 4850
MluI A'CGCG_T
Cuts at: 224 4283 224
Size: 4059 791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 708 951 3521 3705 708
Size: 243 2570 184 1853
Fragments arranged by size:
2570 1853 243 184
MscI TGG'CCA
Cuts at: 2611 2611
Size: 4850
MslI CAynn'nnrTG
Cuts at: 1736 2839 2966 1736
Size: 1103 127 3620
Fragments arranged by size:
3620 1103 127
MspA1I CmG'CkG
Cuts at: 1438 1961 2275 2635 3648 3893 1438
Size: 523 314 360 1013 245 2395
Fragments arranged by size:
2395 1013 523 360 314 245
NaeI GCC'GGC
Cuts at: 3031 3031
Size: 4850
NarI GG'CG_CC
Cuts at: 2281 2529 2281
Size: 248 4602
NgoMIV G'CCGG_C
Cuts at: 3029 3029
Size: 4850
NsiI A_TGCA'T
Cuts at: 902 902
Size: 4850
NspI r_CATG'y
Cuts at: 1115 1903 2403 3310 1115
Size: 788 500 907 2655
Fragments arranged by size:
2655 907 788 500
PciI A'CATG_T
Cuts at: 1111 3306 1111
Size: 2195 2655
PfoI T'CCnGG_A
Cuts at: 660 1203 660
Size: 543 4307
PmeI GTTT'AAAC
Cuts at: 1849 1849
Size: 4850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 439 471 1480 1512 3989 4021 439
Size: 32 1009 32 2477 32 1268
Fragments arranged by size:
2477 1268 1009 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 199 199
Size: 4850
PspOMI G'GGCC_C
Cuts at: 254 1270 254
Size: 1016 3834
PstI C_TGCA'G
Cuts at: 468 1897 2582 468
Size: 1429 685 2736
Fragments arranged by size:
2736 1429 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4850
PvuII CAG'CTG
Cuts at: 2275 2635 2275
Size: 360 4490
RsrII CG'GwC_CG
Cuts at: 3045 3045
Size: 4850
SacI G_AGCT'C
Cuts at: 1864 1864
Size: 4850
SalI G'TCGA_C
Cuts at: 1887 1887
Size: 4850
SanDI GG'GwC_CC
Cuts at: 199 199
Size: 4850
SapI GCTCTTCn'nnn_
Cuts at: 2873 3083 2873
Size: 210 4640
SbfI CC_TGCA'GG
Cuts at: 1897 1897
Size: 4850
ScaI AGT'ACT
Cuts at: 4367 4367
Size: 4850
SfcI C'TryA_G
Cuts at: 33 464 1893 2578 3571 3762 33
Size: 431 1429 685 993 191 1121
Fragments arranged by size:
1429 1121 993 685 431 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 262 262
Size: 4850
SfoI GGC'GCC
Cuts at: 2282 2530 2282
Size: 248 4602
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
SmaI CCC'GGG
Cuts at: 1872 1872
Size: 4850
SmlI C'TyrA_G
Cuts at: 259 645 911 2005 2920 3412 3674 259
Size: 386 266 1094 915 492 262 1435
Fragments arranged by size:
1435 1094 915 492 386 266 262
SpeI A'CTAG_T
Cuts at: 67 408 67
Size: 341 4509
SphI G_CATG'C
Cuts at: 1903 2403 1903
Size: 500 4350
StyI C'CwwG_G
Cuts at: 1223 1984 2338 1223
Size: 761 354 3735
Fragments arranged by size:
3735 761 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 315 733 1254 2433 2566 3025 3234 315
Size: 418 521 1179 133 459 209 1931
Fragments arranged by size:
1931 1179 521 459 418 209 133
TatI w'GTAC_w
Cuts at: 4365 4365
Size: 4850
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1029 1061 4367 4399 1029
Size: 32 3306 32 1480
Fragments arranged by size:
3306 1480 32 32
Tth111I GACn'n_nGTC
Cuts at: 783 2647 783
Size: 1864 2986
XbaI T'CTAG_A
Cuts at: 1881 1881
Size: 4850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2345 2345
Size: 4850
XmaI C'CCGG_G
Cuts at: 1870 1870
Size: 4850
ZraI GAC'GTC
Cuts at: 976 976
Size: 4850
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I BtgI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PasI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI