Restriction map for
pF1KB3911
(Circular) (Six-base) MAPSORT of: pF1KB3911.seq Check: 2749 from: 1 to: 4475
pF1KB3911 4475 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1513 1513
Size: 4475
Acc65I G'GTAC_C
Cuts at: 1491 1491
Size: 4475
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 2300 2732 3085 3747 569
Size: 1731 432 353 662 1297
Fragments arranged by size:
1731 1297 662 432 353
AflIII A'CryG_T
Cuts at: 1827 3627 3908 1827
Size: 1800 281 2394
Fragments arranged by size:
2394 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1780 3218 1780
Size: 1438 3037
ApaLI G'TGCA_C
Cuts at: 171 3313 171
Size: 3142 1333
ApoI r'AATT_y
Cuts at: 308 718 821 1147 1479 3828 4084 308
Size: 410 103 326 332 2349 256 699
Fragments arranged by size:
2349 699 410 332 326 256 103
AseI AT'TA_AT
Cuts at: 20 20
Size: 4475
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 812 1112 1495 812
Size: 300 383 3792
Fragments arranged by size:
3792 383 300
BamHI G'GATC_C
Cuts at: 333 1500 333
Size: 1167 3308
BanI G'GyrC_C
Cuts at: 1491 1905 2153 2188 1491
Size: 414 248 35 3778
Fragments arranged by size:
3778 414 248 35
BanII G_rGCy'C
Cuts at: 1489 2519 1489
Size: 1030 3445
BbeI G_GCGC'C
Cuts at: 1909 2157 1909
Size: 248 4227
BbsI GAAGACnn'nnnn_
Cuts at: 1324 1324
Size: 4475
BciVI GTATCCnnnnn_n'
Cuts at: 644 1324 2373 3429 4475 644
Size: 680 1049 1056 1046 644
Fragments arranged by size:
1056 1049 1046 680 644
BclI T'GATC_A
Cuts at: 903 1995 903
Size: 1092 3383
BglII A'GATC_T
Cuts at: 1990 4027 1990
Size: 2037 2438
BlpI GC'TnA_GC
Cuts at: 1587 1587
Size: 4475
Bme1580I G_kGCm'C
Cuts at: 175 2100 2193 3317 175
Size: 1925 93 1124 1333
Fragments arranged by size:
1925 1333 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1687 1768 2103 4247 1687
Size: 81 335 2144 1915
Fragments arranged by size:
2144 1915 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 841 2842 841
Size: 2001 2474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 849 849
Size: 4475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 1651 2530 3244 3542 515
Size: 1136 879 714 298 1448
Fragments arranged by size:
1448 1136 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4179 4179
Size: 4475
BsaAI yAC'GTr
Cuts at: 1828 1828
Size: 4475
BsaBI GATnn'nnATC
Cuts at: 6 1989 4026 6
Size: 1983 2037 455
Fragments arranged by size:
2037 1983 455
BsaHI Gr'CG_yC
Cuts at: 1906 2154 2856 4352 1906
Size: 248 702 1496 2029
Fragments arranged by size:
2029 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1664 2185 2863 3274 3421 3695 1664
Size: 521 678 411 147 274 2444
Fragments arranged by size:
2444 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 142
Size: 4475
BseYI C'CCAG_C
Cuts at: 1412 1900 2658 3323 3731 1412
Size: 488 758 665 408 2156
Fragments arranged by size:
2156 758 665 488 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 1358 596
Size: 762 3713
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3293 104
Size: 1913 46 1230 1286
Fragments arranged by size:
1913 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 175 1489 2267 2457 3317 175
Size: 1314 778 190 860 1333
Fragments arranged by size:
1333 1314 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 175 1489 2100 2193 2267 2457 2519 3317 175
Size: 1314 611 93 74 190 62 798 1333
Fragments arranged by size:
1333 1314 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 303 2907 303
Size: 2604 1871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 462 1525 1739 2041 2422 462
Size: 1063 214 302 381 2515
Fragments arranged by size:
2515 1063 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2767 2821 4305 4470 50
Size: 2717 54 1484 165 55
Fragments arranged by size:
2717 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 2387
Size: 4475
BsrFI r'CCGG_y
Cuts at: 2473 2654 2473
Size: 181 4294
BsrGI T'GTAC_A
Cuts at: 381 393 1295 381
Size: 12 902 3561
Fragments arranged by size:
3561 902 12
BssSI C'ACGA_G
Cuts at: 2746 3454 2746
Size: 708 3767
BstAPI GCAn_nnn'nTGC
Cuts at: 1083 1852 1083
Size: 769 3706
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstYI r'GATC_y
Cuts at: 1 333 1500 1967 1990 2325 2571 2877 2889
Size: 332 1167 467 23 335 246 306 12
Cuts at: 2889 2975 4027 1
Size: 86 1052 449
Fragments arranged by size:
1167 1052 467 449 335 332 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 1401 1401
Size: 4475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 1718 1718
Size: 4475
DraI TTT'AAA
Cuts at: 260 1474 260
Size: 1214 3261
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3525 2181
Size: 1344 3131
EaeI y'GGCC_r
Cuts at: 1059 2060 2234 2625 2652 3997 1059
Size: 1001 174 391 27 1345 1537
Fragments arranged by size:
1537 1345 1001 391 174 27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 2498 2708 2498
Size: 210 4265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2683 3425 3571 4285 2683
Size: 742 146 714 2873
Fragments arranged by size:
2873 742 714 146
EcoICRI GAG'CTC
Cuts at: 1487 1487
Size: 4475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 849 2300 2732 3085 3747 569
Size: 280 1451 432 353 662 1297
Fragments arranged by size:
1451 1297 662 432 353 280
EcoO109I rG'GnC_Cy
Cuts at: 1614 1614
Size: 4475
EcoRI G'AATT_C
Cuts at: 718 1479 718
Size: 761 3714
EcoRV GAT'ATC
Cuts at: 324 324
Size: 4475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 532 661 2494 532
Size: 129 1833 2513
Fragments arranged by size:
2513 1833 129
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 1006 1909 2157 3387 4158 1006
Size: 903 248 1230 771 1323
Fragments arranged by size:
1323 1230 903 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1015 1047 2317 2347 2349 2379 1015
Size: 32 1270 30 2 30 3111
Fragments arranged by size:
3111 1270 32 30 30 2
HincII GTy'rAC
Cuts at: 1514 1514
Size: 4475
KasI G'GCGC_C
Cuts at: 1905 2153 1905
Size: 248 4227
KpnI G_GTAC'C
Cuts at: 1495 1495
Size: 4475
MfeI C'AATT_G
Cuts at: 899 899
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1092 3234 3418 1092
Size: 2142 184 2149
Fragments arranged by size:
2149 2142 184
MscI TGG'CCA
Cuts at: 1061 2236 1061
Size: 1175 3300
MslI CAynn'nnrTG
Cuts at: 1182 2464 2591 1182
Size: 1282 127 3066
Fragments arranged by size:
3066 1282 127
MspA1I CmG'CkG
Cuts at: 211 330 804 1342 1586 1900 2260 3044 3289
Size: 119 474 538 244 314 360 784 245
Cuts at: 3289 211
Size: 1397
Fragments arranged by size:
1397 784 538 474 360 314 245 244
119
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 4475
NarI GG'CG_CC
Cuts at: 1906 2154 1906
Size: 248 4227
NcoI C'CATG_G
Cuts at: 1401 1401
Size: 4475
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 4475
NspI r_CATG'y
Cuts at: 1528 2028 3631 1528
Size: 500 1603 2372
Fragments arranged by size:
2372 1603 500
PciI A'CATG_T
Cuts at: 3627 3627
Size: 4475
PflMI CCAn_nnn'nTGG
Cuts at: 671 671
Size: 4475
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 4443
PsiI TTA'TAA
Cuts at: 967 967
Size: 4475
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 266 298 266
Size: 32 4443
PstI C_TGCA'G
Cuts at: 1522 2207 1522
Size: 685 3790
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 211 330 1900 2260 211
Size: 119 1570 360 2426
Fragments arranged by size:
2426 1570 360 119
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 1489 1489
Size: 4475
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 2498 2708 2498
Size: 210 4265
SbfI CC_TGCA'GG
Cuts at: 1522 1522
Size: 4475
ScaI AGT'ACT
Cuts at: 3992 3992
Size: 4475
SfcI C'TryA_G
Cuts at: 33 283 624 1518 2203 3171 3362 33
Size: 250 341 894 685 968 191 1146
Fragments arranged by size:
1146 968 894 685 341 250 191
SfoI GGC'GCC
Cuts at: 1907 2155 1907
Size: 248 4227
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 1497 1497
Size: 4475
SmlI C'TyrA_G
Cuts at: 530 1112 1630 2545 3259 3521 530
Size: 582 518 915 714 262 1484
Fragments arranged by size:
1484 915 714 582 518 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1528 2028 1528
Size: 500 3975
StyI C'CwwG_G
Cuts at: 357 411 645 1401 1609 1963 357
Size: 54 234 756 208 354 2869
Fragments arranged by size:
2869 756 354 234 208 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 687 745 2058 2191 2650 2859 687
Size: 58 1313 133 459 209 2303
Fragments arranged by size:
2303 1313 459 209 133 58
TatI w'GTAC_w
Cuts at: 381 393 993 1295 3990 381
Size: 12 600 302 2695 866
Fragments arranged by size:
2695 866 600 302 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3992 4024 3992
Size: 32 4443
Tth111I GACn'n_nGTC
Cuts at: 685 2272 685
Size: 1587 2888
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1970 1970
Size: 4475
XhoI C'TCGA_G
Cuts at: 1112 1112
Size: 4475
XmaI C'CCGG_G
Cuts at: 1495 1495
Size: 4475
XmnI GAAnn'nnTTC
Cuts at: 718 718
Size: 4475
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI
BspEI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI