Restriction map for
pF1KB3906
(Circular) (Six-base) MAPSORT of: pF1KB3906.seq Check: 5862 from: 1 to: 4367
pF1KB3906 4367 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 2192 2624 3371 3639 189
Size: 2003 432 747 268 917
Fragments arranged by size:
2003 917 747 432 268
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 480 877 480
Size: 397 3970
AlwNI CAG_nnn'CTG
Cuts at: 1672 3239 1672
Size: 1567 2800
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 652 1371 3720 3976 652
Size: 719 2349 256 1043
Fragments arranged by size:
2349 1043 719 256
AseI AT'TA_AT
Cuts at: 20 1193 20
Size: 1173 3194
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 161 1387 161
Size: 1226 3141
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 119 157 184 1383 1797 2045 2080 119
Size: 38 27 1199 414 248 35 2406
Fragments arranged by size:
2406 1199 414 248 38 35 27
BanII G_rGCy'C
Cuts at: 292 582 1381 2411 292
Size: 290 799 1030 2248
Fragments arranged by size:
2248 1030 799 290
BbeI G_GCGC'C
Cuts at: 123 188 1801 2049 123
Size: 65 1613 248 2441
Fragments arranged by size:
2441 1613 248 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4333
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4367
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 506 1479 506
Size: 973 3394
Bme1580I G_kGCm'C
Cuts at: 162 308 1992 2085 3141 162
Size: 146 1684 93 1056 1388
Fragments arranged by size:
1684 1388 1056 146 93
BmrI ACTGGGnnnn_n'
Cuts at: 240 285 1579 1660 1995 4139 240
Size: 45 1294 81 335 2144 468
Fragments arranged by size:
2144 1294 468 335 81 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 2422 2914 3212 1543
Size: 879 492 298 2698
Fragments arranged by size:
2698 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 738 1720 738
Size: 982 3385
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 120 185 1798 2046 2748 4244 120
Size: 65 1613 248 702 1496 243
Fragments arranged by size:
1613 1496 702 248 243 65
BsaWI w'CCGG_w
Cuts at: 1556 2077 2755 3029 3176 3587 1556
Size: 521 678 274 147 411 2336
Fragments arranged by size:
2336 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1792 2550 3127 3623 1792
Size: 758 577 496 2536
Fragments arranged by size:
2536 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 394 802 1045 256
Size: 138 408 243 3578
Fragments arranged by size:
3578 408 243 138
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1015 1381 2159 2349 3141 1015
Size: 366 778 190 792 2241
Fragments arranged by size:
2241 792 778 366 190
BsiWI C'GTAC_G
Cuts at: 323 323
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 162 292 308 582 1015 1381 1992 2085 2159
Size: 130 16 274 433 366 611 93 74
Cuts at: 2159 2349 2411 3141 162
Size: 190 62 730 1388
Fragments arranged by size:
1388 730 611 433 366 274 190 130
93 74 62 16
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 3543 3543
Size: 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 1417 1631 1933 2314 278
Size: 1139 214 302 381 2331
Fragments arranged by size:
2331 1139 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 752 2279 752
Size: 1527 2840
BsrFI r'CCGG_y
Cuts at: 2365 2546 2755 2365
Size: 181 209 3977
Fragments arranged by size:
3977 209 181
BsrGI T'GTAC_A
Cuts at: 548 548
Size: 4367
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstYI r'GATC_y
Cuts at: 1 1392 1859 1882 2217 2463 3475 3561 3573
Size: 1391 467 23 335 246 1012 86 12
Cuts at: 3573 3919 1
Size: 346 449
Fragments arranged by size:
1391 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 115 335 115
Size: 220 4147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 375 393 1297 1610 375
Size: 18 904 313 3132
Fragments arranged by size:
3132 904 313 18
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DraIII CAC_nnn'GTG
Cuts at: 234 234
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 431 2073 2931 431
Size: 1642 858 1867
Fragments arranged by size:
1867 1642 858
EaeI y'GGCC_r
Cuts at: 1952 2126 2517 2544 3889 1952
Size: 174 391 27 1345 2430
Fragments arranged by size:
2430 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 280 837 2390 2600 280
Size: 557 1553 210 2047
Fragments arranged by size:
2047 1553 557 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 2192 2624 3371 3639 189
Size: 2003 432 747 268 917
Fragments arranged by size:
2003 917 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 200 331 571 1506 200
Size: 131 240 935 3061
Fragments arranged by size:
3061 935 240 131
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
EcoRV GAT'ATC
Cuts at: 252 252
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 123 188 1801 2049 3071 4050 123
Size: 65 1613 248 1022 979 440
Fragments arranged by size:
1613 1022 979 440 248 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1208 1240 2209 2239 2241 2271 1208
Size: 32 969 30 2 30 3304
Fragments arranged by size:
3304 969 32 30 30 2
HincII GTy'rAC
Cuts at: 1406 1406
Size: 4367
KasI G'GCGC_C
Cuts at: 119 184 1797 2045 119
Size: 65 1613 248 2441
Fragments arranged by size:
2441 1613 248 65
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 1016 3038 3222 197
Size: 819 2022 184 1342
Fragments arranged by size:
2022 1342 819 184
MscI TGG'CCA
Cuts at: 2128 2128
Size: 4367
MslI CAynn'nnrTG
Cuts at: 859 2356 2483 859
Size: 1497 127 2743
Fragments arranged by size:
2743 1497 127
MspA1I CmG'CkG
Cuts at: 117 396 1309 1478 1792 2152 3165 3410 117
Size: 279 913 169 314 360 1013 245 1074
Fragments arranged by size:
1074 1013 913 360 314 279 245 169
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 120 185 1798 2046 120
Size: 65 1613 248 2441
Fragments arranged by size:
2441 1613 248 65
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4367
NspI r_CATG'y
Cuts at: 1306 1420 1920 2827 1306
Size: 114 500 907 2846
Fragments arranged by size:
2846 907 500 114
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PpuMI rG'GwC_Cy
Cuts at: 200 571 200
Size: 371 3996
PsiI TTA'TAA
Cuts at: 1265 1285 1265
Size: 20 4347
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 701 733 701
Size: 32 4335
PstI C_TGCA'G
Cuts at: 1414 2099 1414
Size: 685 3682
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 396 1309 1792 2152 396
Size: 913 483 360 2611
Fragments arranged by size:
2611 913 483 360
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SacII CC_GC'GG
Cuts at: 118 118
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 280 2390 2600 280
Size: 2110 210 2047
Fragments arranged by size:
2110 2047 210
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 1410 2095 3088 3279 33
Size: 1377 685 993 191 1121
Fragments arranged by size:
1377 1121 993 685 191
SfoI GGC'GCC
Cuts at: 121 186 1799 2047 121
Size: 65 1613 248 2441
Fragments arranged by size:
2441 1613 248 65
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 163 1389 163
Size: 1226 3141
SmlI C'TyrA_G
Cuts at: 1522 2437 2929 3191 1522
Size: 915 492 262 2698
Fragments arranged by size:
2698 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1306 1420 1920 1306
Size: 114 500 3753
Fragments arranged by size:
3753 500 114
SspI AAT'ATT
Cuts at: 543 543
Size: 4367
StyI C'CwwG_G
Cuts at: 667 1170 1501 1855 667
Size: 503 331 354 3179
Fragments arranged by size:
3179 503 354 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 222 1950 2083 2542 2751 222
Size: 1728 133 459 209 1838
Fragments arranged by size:
1838 1728 459 209 133
TatI w'GTAC_w
Cuts at: 548 601 1292 3882 548
Size: 53 691 2590 1033
Fragments arranged by size:
2590 1033 691 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4335
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 161 1387 161
Size: 1226 3141
XmnI GAAnn'nnTTC
Cuts at: 713 1184 713
Size: 471 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PmeI PpiI PpuMI PsiI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PshAI PspOMI PspXI
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI