Restriction map for
pF1KB3903
(Circular) (Six-base) MAPSORT of: pF1KB3903.seq Check: 9939 from: 1 to: 5411
pF1KB3903 5411 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1558 2449 1558
Size: 891 4520
Acc65I G'GTAC_C
Cuts at: 2427 2427
Size: 5411
AclI AA'CG_TT
Cuts at: 5251 5251
Size: 5411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1049 1256 1837 1987 3236 3668 4021 4683
Size: 49 207 581 150 1249 432 353 662
Cuts at: 4683 1000
Size: 1728
Fragments arranged by size:
1728 1249 662 581 432 353 207 150
49
AfeI AGC'GCT
Cuts at: 635 635
Size: 5411
AflIII A'CryG_T
Cuts at: 539 2763 4563 4844 539
Size: 2224 1800 281 1106
Fragments arranged by size:
2224 1800 1106 281
AleI CACnn'nnGTG
Cuts at: 1777 1777
Size: 5411
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1187 1187
Size: 5411
AlwNI CAG_nnn'CTG
Cuts at: 1495 2716 4154 1495
Size: 1221 1438 2752
Fragments arranged by size:
2752 1438 1221
ApaI G_GGCC'C
Cuts at: 189 223 1040 189
Size: 34 817 4560
Fragments arranged by size:
4560 817 34
ApaLI G'TGCA_C
Cuts at: 1642 4249 1642
Size: 2607 2804
ApoI r'AATT_y
Cuts at: 925 2415 4764 5020 925
Size: 1490 2349 256 1316
Fragments arranged by size:
2349 1490 1316 256
AseI AT'TA_AT
Cuts at: 20 590 1987 20
Size: 570 1397 3444
Fragments arranged by size:
3444 1397 570
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5411
AvaI C'yCGr_G
Cuts at: 216 629 2364 2431 216
Size: 413 1735 67 3196
Fragments arranged by size:
3196 1735 413 67
BamHI G'GATC_C
Cuts at: 746 1021 2436 746
Size: 275 1415 3721
Fragments arranged by size:
3721 1415 275
BanI G'GyrC_C
Cuts at: 2390 2427 2841 3089 3124 2390
Size: 37 414 248 35 4677
Fragments arranged by size:
4677 414 248 37 35
BanII G_rGCy'C
Cuts at: 189 223 239 425 1040 1502 2258 2425 3455
Size: 34 16 186 615 462 756 167 1030
Cuts at: 3455 189
Size: 2145
Fragments arranged by size:
2145 1030 756 615 462 186 167 34
16
BbeI G_GCGC'C
Cuts at: 2394 2845 3093 2394
Size: 451 248 4712
Fragments arranged by size:
4712 451 248
BbvCI CC'TCA_GC
Cuts at: 301 1002 1633 2096 301
Size: 701 631 463 3616
Fragments arranged by size:
3616 701 631 463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1535 1569 1535
Size: 34 5377
BciVI GTATCCnnnnn_n'
Cuts at: 3309 4365 5411 3309
Size: 1056 1046 3309
Fragments arranged by size:
3309 1056 1046
BclI T'GATC_A
Cuts at: 1844 2931 1844
Size: 1087 4324
BglI GCCn_nnn'nGGC
Cuts at: 325 325
Size: 5411
BglII A'GATC_T
Cuts at: 1183 1533 1905 1919 2926 4963 1183
Size: 350 372 14 1007 2037 1631
Fragments arranged by size:
2037 1631 1007 372 350 14
BlpI GC'TnA_GC
Cuts at: 2523 2523
Size: 5411
Bme1580I G_kGCm'C
Cuts at: 189 223 264 1040 1646 3036 3129 4253 189
Size: 34 41 776 606 1390 93 1124 1347
Fragments arranged by size:
1390 1347 1124 776 606 93 41 34
BmrI ACTGGGnnnn_n'
Cuts at: 239 1720 1965 2623 2704 3039 5183 239
Size: 1481 245 658 81 335 2144 467
Fragments arranged by size:
2144 1481 658 467 335 245 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2277 3778 2277
Size: 1501 3910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 366 1104 1769 1882 1957 2249 154
Size: 212 738 665 113 75 292 3316
Fragments arranged by size:
3316 738 665 292 212 113 75
Bpu10I CC'TnA_GC
Cuts at: 301 1002 1211 1633 2096 301
Size: 701 209 422 463 3616
Fragments arranged by size:
3616 701 463 422 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 2587 3466 4180 4478 1300
Size: 1287 879 714 298 2233
Fragments arranged by size:
2233 1287 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2172 5115 2172
Size: 2943 2468
BsaAI yAC'GTr
Cuts at: 540 2764 540
Size: 2224 3187
BsaBI GATnn'nnATC
Cuts at: 6 2925 4962 6
Size: 2919 2037 455
Fragments arranged by size:
2919 2037 455
BsaHI Gr'CG_yC
Cuts at: 2391 2842 3090 3792 5288 2391
Size: 451 248 702 1496 2514
Fragments arranged by size:
2514 1496 702 451 248
BsaWI w'CCGG_w
Cuts at: 338 395 2328 2600 3121 3799 4210 4357 4631
Size: 57 1933 272 521 678 411 147 274
Cuts at: 4631 338
Size: 1118
Fragments arranged by size:
1933 1118 678 521 411 274 272 147
57
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 403 639 1492 2299 186
Size: 217 236 853 807 3298
Fragments arranged by size:
3298 853 807 236 217
BseYI C'CCAG_C
Cuts at: 190 1081 1927 2836 3594 4259 4667 190
Size: 891 846 909 758 665 408 934
Fragments arranged by size:
934 909 891 846 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 828 828
Size: 5411
BsiEI CG_ry'CG
Cuts at: 104 169 390 2953 2999 4229 104
Size: 65 221 2563 46 1230 1286
Fragments arranged by size:
2563 1286 1230 221 65 46
BsiHKAI G_wGCw'C
Cuts at: 140 425 1502 1646 2425 3203 3393 4253 140
Size: 285 1077 144 779 778 190 860 1298
Fragments arranged by size:
1298 1077 860 779 778 285 190 144
BsmBI CGTCTCn'nnnn_
Cuts at: 2396 2396
Size: 5411
Bsp1286I G_dGCh'C
Cuts at: 140 189 223 239 264 425 1040 1502 1646
Size: 49 34 16 25 161 615 462 144
Cuts at: 1646 2258 2425 3036 3129 3203 3393 3455 4253
Size: 612 167 611 93 74 190 62 798
Cuts at: 4253 140
Size: 1298
Fragments arranged by size:
1298 798 615 612 611 462 190 167
161 144 93 74 62 49 34 25
16
BspEI T'CCGG_A
Cuts at: 2328 2328
Size: 5411
BspHI T'CATG_A
Cuts at: 2177 3843 2177
Size: 1666 3745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 461 852 2461 2675 2977 3358 461
Size: 391 1609 214 302 381 2514
Fragments arranged by size:
2514 1609 391 381 302 214
BsrBI CCG'CTC
Cuts at: 50 431 2376 3703 3757 5241 5406 50
Size: 381 1945 1327 54 1484 165 55
Fragments arranged by size:
1945 1484 1327 381 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1793 3323 1793
Size: 1530 3881
BsrFI r'CCGG_y
Cuts at: 3409 3590 3409
Size: 181 5230
BsrGI T'GTAC_A
Cuts at: 1595 1595
Size: 5411
BssHII G'CGCG_C
Cuts at: 328 328
Size: 5411
BssSI C'ACGA_G
Cuts at: 3682 4390 3682
Size: 708 4703
BstAPI GCAn_nnn'nTGC
Cuts at: 2788 2788
Size: 5411
BstBI TT'CG_AA
Cuts at: 714 3772 714
Size: 3058 2353
BstEII G'GTnAC_C
Cuts at: 501 501
Size: 5411
BstXI CCAn_nnnn'nTGG
Cuts at: 1779 1779
Size: 5411
BstYI r'GATC_y
Cuts at: 1 746 1021 1183 1533 1905 1919 2288 2436
Size: 745 275 162 350 372 14 369 148
Cuts at: 2436 2903 2926 3261 3507 3813 3825 3911 4963
Size: 467 23 335 246 306 12 86 1052
Cuts at: 4963 1
Size: 449
Fragments arranged by size:
1052 745 467 449 372 369 350 335
306 275 246 162 148 86 23 14
12
Bsu36I CC'TnA_GG
Cuts at: 1615 1615
Size: 5411
BtgI C'CryG_G
Cuts at: 107 163 1589 1626 2030 107
Size: 56 1426 37 404 3488
Fragments arranged by size:
3488 1426 404 56 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3361 3542 3705 3361
Size: 181 163 5067
Fragments arranged by size:
5067 181 163
BtsI GCAGTG_nn'
Cuts at: 264 1590 2654 264
Size: 1326 1064 3021
Fragments arranged by size:
3021 1326 1064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1380 1415 1380
Size: 35 5376
DraI TTT'AAA
Cuts at: 2410 2410
Size: 5411
DrdI GACnn_nn'nnGTC
Cuts at: 826 3117 4461 826
Size: 2291 1344 1776
Fragments arranged by size:
2291 1776 1344
EaeI y'GGCC_r
Cuts at: 110 166 2996 3170 3561 3588 4933 110
Size: 56 2830 174 391 27 1345 588
Fragments arranged by size:
2830 1345 588 391 174 56 27
EagI C'GGCC_G
Cuts at: 166 2996 166
Size: 2830 2581
EarI CTCTTCn'nnn_
Cuts at: 1280 1915 3434 3644 1280
Size: 635 1519 210 3047
Fragments arranged by size:
3047 1519 635 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3619 4361 4507 5221 3619
Size: 742 146 714 3809
Fragments arranged by size:
3809 742 714 146
EcoICRI GAG'CTC
Cuts at: 423 1500 2423 423
Size: 1077 923 3411
Fragments arranged by size:
3411 1077 923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 366 1000 1049 1104 1256 1769 1837 1882
Size: 212 634 49 55 152 513 68 45
Cuts at: 1882 1957 1987 2249 3236 3668 4021 4683 154
Size: 75 30 262 987 432 353 662 882
Fragments arranged by size:
987 882 662 634 513 432 353 262
212 152 75 68 55 49 45 30
EcoNI CCTnn'n_nnAGG
Cuts at: 457 457
Size: 5411
EcoO109I rG'GnC_Cy
Cuts at: 185 186 220 297 363 1037 1744 2550 185
Size: 1 34 77 66 674 707 806 3046
Fragments arranged by size:
3046 806 707 674 77 66 34 1
EcoRI G'AATT_C
Cuts at: 2415 2415
Size: 5411
EcoRV GAT'ATC
Cuts at: 798 798
Size: 5411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 348 3430 348
Size: 3082 2329
FspI TGC'GCA
Cuts at: 3192 3192
Size: 5411
HaeII r_GCGC'y
Cuts at: 637 2394 2845 3093 4323 5094 637
Size: 1757 451 248 1230 771 954
Fragments arranged by size:
1757 1230 954 771 451 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1158 1190 2056 2088 3253 3283 3285 3315 1158
Size: 32 866 32 1165 30 2 30 3254
Fragments arranged by size:
3254 1165 866 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1559 2450 1559
Size: 891 4520
KasI G'GCGC_C
Cuts at: 2390 2841 3089 2390
Size: 451 248 4712
Fragments arranged by size:
4712 451 248
KpnI G_GTAC'C
Cuts at: 2431 2431
Size: 5411
MluI A'CGCG_T
Cuts at: 4844 4844
Size: 5411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 419 1156 4170 4354 419
Size: 737 3014 184 1476
Fragments arranged by size:
3014 1476 737 184
MscI TGG'CCA
Cuts at: 112 3172 112
Size: 3060 2351
MslI CAynn'nnrTG
Cuts at: 1777 3400 3527 1777
Size: 1623 127 3661
Fragments arranged by size:
3661 1623 127
MspA1I CmG'CkG
Cuts at: 165 1006 1837 2032 2237 2359 2522 2836 3196
Size: 841 831 195 205 122 163 314 360
Cuts at: 3196 3980 4225 165
Size: 784 245 1351
Fragments arranged by size:
1351 841 831 784 360 314 245 205
195 163 122
NaeI GCC'GGC
Cuts at: 3592 3592
Size: 5411
NarI GG'CG_CC
Cuts at: 2391 2842 3090 2391
Size: 451 248 4712
Fragments arranged by size:
4712 451 248
NcoI C'CATG_G
Cuts at: 107 1589 1626 107
Size: 1482 37 3892
Fragments arranged by size:
3892 1482 37
NgoMIV G'CCGG_C
Cuts at: 3590 3590
Size: 5411
NspI r_CATG'y
Cuts at: 914 2091 2464 2964 4567 914
Size: 1177 373 500 1603 1758
Fragments arranged by size:
1758 1603 1177 500 373
PasI CC'CwG_GG
Cuts at: 459 1033 2054 459
Size: 574 1021 3816
Fragments arranged by size:
3816 1021 574
PciI A'CATG_T
Cuts at: 4563 4563
Size: 5411
PflMI CCAn_nnn'nTGG
Cuts at: 1923 1923
Size: 5411
PfoI T'CCnGG_A
Cuts at: 953 1140 1898 953
Size: 187 758 4466
Fragments arranged by size:
4466 758 187
PmeI GTTT'AAAC
Cuts at: 2410 2410
Size: 5411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 3857
Size: 32 5379
PspOMI G'GGCC_C
Cuts at: 185 219 1036 185
Size: 34 817 4560
Fragments arranged by size:
4560 817 34
PstI C_TGCA'G
Cuts at: 271 296 2218 2458 3143 271
Size: 25 1922 240 685 2539
Fragments arranged by size:
2539 1922 685 240 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5411
PvuII CAG'CTG
Cuts at: 1006 1837 2836 3196 1006
Size: 831 999 360 3221
Fragments arranged by size:
3221 999 831 360
RsrII CG'GwC_CG
Cuts at: 3606 3606
Size: 5411
SacI G_AGCT'C
Cuts at: 425 1502 2425 425
Size: 1077 923 3411
Fragments arranged by size:
3411 1077 923
SacII CC_GC'GG
Cuts at: 166 2033 166
Size: 1867 3544
SalI G'TCGA_C
Cuts at: 1557 2448 1557
Size: 891 4520
SapI GCTCTTCn'nnn_
Cuts at: 3434 3644 3434
Size: 210 5201
SbfI CC_TGCA'GG
Cuts at: 296 2458 296
Size: 2162 3249
ScaI AGT'ACT
Cuts at: 4928 4928
Size: 5411
SfcI C'TryA_G
Cuts at: 33 267 292 2214 2454 3139 4107 4298 33
Size: 234 25 1922 240 685 968 191 1146
Fragments arranged by size:
1922 1146 968 685 240 234 191 25
SfoI GGC'GCC
Cuts at: 2392 2843 3091 2392
Size: 451 248 4712
Fragments arranged by size:
4712 451 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5411
SmaI CCC'GGG
Cuts at: 2366 2433 2366
Size: 67 5344
SmlI C'TyrA_G
Cuts at: 1279 2566 3481 4195 4457 1279
Size: 1287 915 714 262 2233
Fragments arranged by size:
2233 1287 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1135 67
Size: 1068 4343
SphI G_CATG'C
Cuts at: 2464 2964 2464
Size: 500 4911
SspI AAT'ATT
Cuts at: 588 588
Size: 5411
StuI AGG'CCT
Cuts at: 2244 2244
Size: 5411
StyI C'CwwG_G
Cuts at: 107 224 1589 1626 1979 2545 2899 107
Size: 117 1365 37 353 566 354 2619
Fragments arranged by size:
2619 1365 566 354 353 117 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1785 2046 2994 3127 3586 3795 1785
Size: 261 948 133 459 209 3401
Fragments arranged by size:
3401 948 459 261 209 133
TatI w'GTAC_w
Cuts at: 1565 1595 4926 1565
Size: 30 3331 2050
Fragments arranged by size:
3331 2050 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4928 4960 4928
Size: 32 5379
Tth111I GACn'n_nGTC
Cuts at: 949 3208 949
Size: 2259 3152
XbaI T'CTAG_A
Cuts at: 2190 2442 2190
Size: 252 5159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1924 2906 1924
Size: 982 4429
XmaI C'CCGG_G
Cuts at: 2364 2431 2364
Size: 67 5344
XmnI GAAnn'nnTTC
Cuts at: 560 926 560
Size: 366 5045
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BstZ17I ClaI DraIII FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PpuMI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI