Restriction map for
pF1KB3902
(Circular) (Six-base) MAPSORT of: pF1KB3902.seq Check: 5044 from: 1 to: 4949
pF1KB3902 4949 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 1935 1935
Size: 4949
AccI GT'mk_AC
Cuts at: 1010 1479 1987 1010
Size: 469 508 3972
Fragments arranged by size:
3972 508 469
Acc65I G'GTAC_C
Cuts at: 1965 1965
Size: 4949
AclI AA'CG_TT
Cuts at: 4789 4789
Size: 4949
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2774 3206 3559 4221 2774
Size: 432 353 662 3502
Fragments arranged by size:
3502 662 432 353
AflIII A'CryG_T
Cuts at: 1023 1688 2301 4101 4382 1023
Size: 665 613 1800 281 1590
Fragments arranged by size:
1800 1590 665 613 281
AhdI GACnn_n'nnGTC
Cuts at: 557 557
Size: 4949
AleI CACnn'nnGTG
Cuts at: 534 534
Size: 4949
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1775 1807 1775
Size: 32 4917
AlwNI CAG_nnn'CTG
Cuts at: 555 1131 2254 3692 555
Size: 576 1123 1438 1812
Fragments arranged by size:
1812 1438 1123 576
ApaI G_GGCC'C
Cuts at: 1601 1601
Size: 4949
ApaLI G'TGCA_C
Cuts at: 3787 3787
Size: 4949
ApoI r'AATT_y
Cuts at: 1489 1953 4302 4558 1489
Size: 464 2349 256 1880
Fragments arranged by size:
2349 1880 464 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4949
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
AvaI C'yCGr_G
Cuts at: 1969 1969
Size: 4949
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 300 333 300
Size: 33 4916
BamHI G'GATC_C
Cuts at: 1974 1974
Size: 4949
BanI G'GyrC_C
Cuts at: 1965 2379 2627 2662 1965
Size: 414 248 35 4252
Fragments arranged by size:
4252 414 248 35
BanII G_rGCy'C
Cuts at: 190 410 1304 1601 1963 2993 190
Size: 220 894 297 362 1030 2146
Fragments arranged by size:
2146 1030 894 362 297 220
BbeI G_GCGC'C
Cuts at: 2383 2631 2383
Size: 248 4701
BbsI GAAGACnn'nnnn_
Cuts at: 370 553 1394 370
Size: 183 841 3925
Fragments arranged by size:
3925 841 183
BciVI GTATCCnnnnn_n'
Cuts at: 1413 2847 3903 4949 1413
Size: 1434 1056 1046 1413
Fragments arranged by size:
1434 1413 1056 1046
BclI T'GATC_A
Cuts at: 1463 2469 1463
Size: 1006 3943
BglII A'GATC_T
Cuts at: 2464 4501 2464
Size: 2037 2912
BlpI GC'TnA_GC
Cuts at: 2061 2061
Size: 4949
Bme1580I G_kGCm'C
Cuts at: 1264 1601 2574 2667 3791 1264
Size: 337 973 93 1124 2422
Fragments arranged by size:
2422 1124 973 337 93
BmgBI CAC'GTC
Cuts at: 756 956 756
Size: 200 4749
BmrI ACTGGGnnnn_n'
Cuts at: 854 1267 1644 2161 2242 2577 4721 854
Size: 413 377 517 81 335 2144 1082
Fragments arranged by size:
2144 1082 517 413 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1551 3316 1551
Size: 1765 3184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1078 1057
Size: 21 4928
Bpu10I CC'TnA_GC
Cuts at: 1046 1046
Size: 4949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 1279 2125 3004 3718 4016 321
Size: 958 846 879 714 298 1254
Fragments arranged by size:
1254 958 879 846 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4653 4653
Size: 4949
BsaAI yAC'GTr
Cuts at: 1024 1691 2302 1024
Size: 667 611 3671
Fragments arranged by size:
3671 667 611
BsaBI GATnn'nnATC
Cuts at: 6 2463 4500 6
Size: 2457 2037 455
Fragments arranged by size:
2457 2037 455
BsaHI Gr'CG_yC
Cuts at: 1932 2380 2628 3330 4826 1932
Size: 448 248 702 1496 2055
Fragments arranged by size:
2055 1496 702 448 248
BsaWI w'CCGG_w
Cuts at: 415 2138 2659 3337 3748 3895 4169 415
Size: 1723 521 678 411 147 274 1195
Fragments arranged by size:
1723 1195 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1054 1543 1054
Size: 489 4460
BseYI C'CCAG_C
Cuts at: 126 159 692 2374 3132 3797 4205 126
Size: 33 533 1682 758 665 408 870
Fragments arranged by size:
1682 870 758 665 533 408 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1090 1099 1534 1090
Size: 9 435 4505
Fragments arranged by size:
4505 435 9
BsiEI CG_ry'CG
Cuts at: 104 250 2491 2537 3767 104
Size: 146 2241 46 1230 1286
Fragments arranged by size:
2241 1286 1230 146 46
BsiHKAI G_wGCw'C
Cuts at: 410 1064 1318 1963 2741 2931 3791 410
Size: 654 254 645 778 190 860 1568
Fragments arranged by size:
1568 860 778 654 645 254 190
BsiWI C'GTAC_G
Cuts at: 768 768
Size: 4949
BsmI GAATG_Cn'
Cuts at: 458 458
Size: 4949
Bsp1286I G_dGCh'C
Cuts at: 190 410 1064 1264 1304 1318 1601 1963 2574
Size: 220 654 200 40 14 283 362 611
Cuts at: 2574 2667 2741 2931 2993 3791 190
Size: 93 74 190 62 798 1348
Fragments arranged by size:
1348 798 654 611 362 283 220 200
190 93 74 62 40 14
BspHI T'CATG_A
Cuts at: 1460 3381 1460
Size: 1921 3028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1999 2213 2515 2896 1999
Size: 214 302 381 4052
Fragments arranged by size:
4052 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3241 3295 4779 4944 50
Size: 3191 54 1484 165 55
Fragments arranged by size:
3191 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 309 807 959 993 1241 2861 309
Size: 498 152 34 248 1620 2397
Fragments arranged by size:
2397 1620 498 248 152 34
BsrFI r'CCGG_y
Cuts at: 242 518 2947 3128 242
Size: 276 2429 181 2063
Fragments arranged by size:
2429 2063 276 181
BssSI C'ACGA_G
Cuts at: 534 3220 3928 534
Size: 2686 708 1555
Fragments arranged by size:
2686 1555 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1113 1131 2326 1113
Size: 18 1195 3736
Fragments arranged by size:
3736 1195 18
BstBI TT'CG_AA
Cuts at: 3310 3310
Size: 4949
BstYI r'GATC_y
Cuts at: 1 1974 2441 2464 2799 3045 3351 3363 3449
Size: 1973 467 23 335 246 306 12 86
Cuts at: 3449 4501 1
Size: 1052 449
Fragments arranged by size:
1973 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1787 1787
Size: 4949
BtgI C'CryG_G
Cuts at: 107 395 1508 1563 1887 107
Size: 288 1113 55 324 3169
Fragments arranged by size:
3169 1113 324 288 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2899 3080 3243 2899
Size: 181 163 4605
Fragments arranged by size:
4605 181 163
BtsI GCAGTG_nn'
Cuts at: 1062 1546 2192 1062
Size: 484 646 3819
Fragments arranged by size:
3819 646 484
DraI TTT'AAA
Cuts at: 1948 1948
Size: 4949
DrdI GACnn_nn'nnGTC
Cuts at: 838 1609 1693 2655 3999 838
Size: 771 84 962 1344 1788
Fragments arranged by size:
1788 1344 962 771 84
EaeI y'GGCC_r
Cuts at: 247 1284 2534 2708 3099 3126 4471 247
Size: 1037 1250 174 391 27 1345 725
Fragments arranged by size:
1345 1250 1037 725 391 174 27
EagI C'GGCC_G
Cuts at: 247 2534 247
Size: 2287 2662
EarI CTCTTCn'nnn_
Cuts at: 271 550 709 1204 1459 2972 3182 271
Size: 279 159 495 255 1513 210 2038
Fragments arranged by size:
2038 1513 495 279 255 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 162 3157 3899 4045 4759 162
Size: 2995 742 146 714 352
Fragments arranged by size:
2995 742 714 352 146
EcoICRI GAG'CTC
Cuts at: 408 1961 408
Size: 1553 3396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1078 2774 3206 3559 4221 1057
Size: 21 1696 432 353 662 1785
Fragments arranged by size:
1785 1696 662 432 353 21
EcoO109I rG'GnC_Cy
Cuts at: 1504 2088 1504
Size: 584 4365
EcoRI G'AATT_C
Cuts at: 1953 1953
Size: 4949
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1320 2968 1320
Size: 1648 3301
FspI TGC'GCA
Cuts at: 2730 2730
Size: 4949
HaeII r_GCGC'y
Cuts at: 2383 2631 3861 4632 2383
Size: 248 1230 771 2700
Fragments arranged by size:
2700 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 543 575 2791 2821 2823 2853 543
Size: 32 2216 30 2 30 2639
Fragments arranged by size:
2639 2216 32 30 30 2
HincII GTy'rAC
Cuts at: 759 1480 1988 759
Size: 721 508 3720
Fragments arranged by size:
3720 721 508
KasI G'GCGC_C
Cuts at: 2379 2627 2379
Size: 248 4701
KpnI G_GTAC'C
Cuts at: 1969 1969
Size: 4949
MfeI C'AATT_G
Cuts at: 237 237
Size: 4949
MluI A'CGCG_T
Cuts at: 4382 4382
Size: 4949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 557 1395 3708 3892 557
Size: 838 2313 184 1614
Fragments arranged by size:
2313 1614 838 184
MscI TGG'CCA
Cuts at: 1286 2710 1286
Size: 1424 3525
MslI CAynn'nnrTG
Cuts at: 534 1153 1618 2938 3065 534
Size: 619 465 1320 127 2418
Fragments arranged by size:
2418 1320 619 465 127
MspA1I CmG'CkG
Cuts at: 602 614 2060 2374 2734 3518 3763 602
Size: 12 1446 314 360 784 245 1788
Fragments arranged by size:
1788 1446 784 360 314 245 12
NaeI GCC'GGC
Cuts at: 244 3130 244
Size: 2886 2063
NarI GG'CG_CC
Cuts at: 2380 2628 2380
Size: 248 4701
NcoI C'CATG_G
Cuts at: 107 1887 107
Size: 1780 3169
NgoMIV G'CCGG_C
Cuts at: 242 3128 242
Size: 2886 2063
NspI r_CATG'y
Cuts at: 2002 2502 4105 2002
Size: 500 1603 2846
Fragments arranged by size:
2846 1603 500
PciI A'CATG_T
Cuts at: 4101 4101
Size: 4949
PfoI T'CCnGG_A
Cuts at: 275 1863 275
Size: 1588 3361
PmeI GTTT'AAAC
Cuts at: 1948 1948
Size: 4949
PmlI CAC'GTG
Cuts at: 1024 1691 1024
Size: 667 4282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 4917
PpuMI rG'GwC_Cy
Cuts at: 1504 1504
Size: 4949
PspOMI G'GGCC_C
Cuts at: 1597 1597
Size: 4949
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1636 1668 1636
Size: 32 4917
PstI C_TGCA'G
Cuts at: 223 601 854 1996 2681 223
Size: 378 253 1142 685 2491
Fragments arranged by size:
2491 1142 685 378 253
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4949
PvuII CAG'CTG
Cuts at: 602 2374 2734 602
Size: 1772 360 2817
Fragments arranged by size:
2817 1772 360
RsrII CG'GwC_CG
Cuts at: 3144 3144
Size: 4949
SacI G_AGCT'C
Cuts at: 410 1963 410
Size: 1553 3396
SalI G'TCGA_C
Cuts at: 1478 1986 1478
Size: 508 4441
SanDI GG'GwC_CC
Cuts at: 1504 1504
Size: 4949
SapI GCTCTTCn'nnn_
Cuts at: 550 1204 2972 3182 550
Size: 654 1768 210 2317
Fragments arranged by size:
2317 1768 654 210
SbfI CC_TGCA'GG
Cuts at: 1996 1996
Size: 4949
ScaI AGT'ACT
Cuts at: 1654 4466 1654
Size: 2812 2137
SfcI C'TryA_G
Cuts at: 33 219 597 850 1992 2677 3645 3836 33
Size: 186 378 253 1142 685 968 191 1146
Fragments arranged by size:
1146 1142 968 685 378 253 191 186
SfoI GGC'GCC
Cuts at: 2381 2629 2381
Size: 248 4701
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
SmaI CCC'GGG
Cuts at: 1971 1971
Size: 4949
SmlI C'TyrA_G
Cuts at: 336 1294 2104 3019 3733 3995 336
Size: 958 810 915 714 262 1290
Fragments arranged by size:
1290 958 915 810 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4949
SphI G_CATG'C
Cuts at: 2002 2502 2002
Size: 500 4449
StyI C'CwwG_G
Cuts at: 107 1816 1887 2083 2437 107
Size: 1709 71 196 354 2619
Fragments arranged by size:
2619 1709 354 196 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1228 2532 2665 3124 3333 1228
Size: 1304 133 459 209 2844
Fragments arranged by size:
2844 1304 459 209 133
TatI w'GTAC_w
Cuts at: 1652 4464 1652
Size: 2812 2137
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4466 4498 4466
Size: 32 4917
Tth111I GACn'n_nGTC
Cuts at: 2746 2746
Size: 4949
XbaI T'CTAG_A
Cuts at: 1980 1980
Size: 4949
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1106 1222 1254 1279 2444 1106
Size: 116 32 25 1165 3611
Fragments arranged by size:
3611 1165 116 32 25
XmaI C'CCGG_G
Cuts at: 1969 1969
Size: 4949
XmnI GAAnn'nnTTC
Cuts at: 265 589 265
Size: 324 4625
ZraI GAC'GTC
Cuts at: 1933 1933
Size: 4949
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AlfI AscI AvrII BbvCI
BcgI BfrBI BglI BmtI BsaXI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PshAI PsiI PspXI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI