Restriction map for
pF1KB3881
(Circular) (Six-base) MAPSORT of: pF1KB3881.seq Check: 5980 from: 1 to: 4220
pF1KB3881 4220 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 758 1258 758
Size: 500 3720
Acc65I G'GTAC_C
Cuts at: 1236 1236
Size: 4220
AclI AA'CG_TT
Cuts at: 4060 4060
Size: 4220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 733 841 2045 2477 2830 3492 433
Size: 300 108 1204 432 353 662 1161
Fragments arranged by size:
1204 1161 662 432 353 300 108
AflIII A'CryG_T
Cuts at: 1572 3372 3653 1572
Size: 1800 281 2139
Fragments arranged by size:
2139 1800 281
AleI CACnn'nnGTG
Cuts at: 424 862 424
Size: 438 3782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 136 997 136
Size: 861 3359
AlwNI CAG_nnn'CTG
Cuts at: 1525 2963 1525
Size: 1438 2782
ApaLI G'TGCA_C
Cuts at: 208 565 1012 3058 208
Size: 357 447 2046 1370
Fragments arranged by size:
2046 1370 447 357
ApoI r'AATT_y
Cuts at: 1224 3573 3829 1224
Size: 2349 256 1615
Fragments arranged by size:
2349 1615 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4220
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
AvaI C'yCGr_G
Cuts at: 1240 1240
Size: 4220
BamHI G'GATC_C
Cuts at: 722 749 1245 722
Size: 27 496 3697
Fragments arranged by size:
3697 496 27
BanI G'GyrC_C
Cuts at: 1236 1650 1898 1933 1236
Size: 414 248 35 3523
Fragments arranged by size:
3523 414 248 35
BanII G_rGCy'C
Cuts at: 1090 1234 2264 1090
Size: 144 1030 3046
Fragments arranged by size:
3046 1030 144
BbeI G_GCGC'C
Cuts at: 1654 1902 1654
Size: 248 3972
BbvCI CC'TCA_GC
Cuts at: 341 341
Size: 4220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 551 585 551
Size: 34 4186
BciVI GTATCCnnnnn_n'
Cuts at: 2118 3174 4220 2118
Size: 1056 1046 2118
Fragments arranged by size:
2118 1056 1046
BclI T'GATC_A
Cuts at: 1740 1740
Size: 4220
BglII A'GATC_T
Cuts at: 1735 3772 1735
Size: 2037 2183
BlpI GC'TnA_GC
Cuts at: 1332 1332
Size: 4220
Bme1580I G_kGCm'C
Cuts at: 212 569 1016 1845 1938 3062 212
Size: 357 447 829 93 1124 1370
Fragments arranged by size:
1370 1124 829 447 357 93
BmgBI CAC'GTC
Cuts at: 999 1004 1011 999
Size: 5 7 4208
Fragments arranged by size:
4208 7 5
BmrI ACTGGGnnnn_n'
Cuts at: 689 1432 1513 1848 3992 689
Size: 743 81 335 2144 917
Fragments arranged by size:
2144 917 743 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 795 2587 795
Size: 1792 2428
Bpu10I CC'TnA_GC
Cuts at: 341 341
Size: 4220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1396 2275 2989 3287 976
Size: 420 879 714 298 1909
Fragments arranged by size:
1909 879 714 420 298
BsaI GGTCTCn'nnnn_
Cuts at: 3924 3924
Size: 4220
BsaAI yAC'GTr
Cuts at: 180 1573 180
Size: 1393 2827
BsaBI GATnn'nnATC
Cuts at: 6 1734 3771 6
Size: 1728 2037 455
Fragments arranged by size:
2037 1728 455
BsaHI Gr'CG_yC
Cuts at: 1121 1651 1899 2601 4097 1121
Size: 530 248 702 1496 1244
Fragments arranged by size:
1496 1244 702 530 248
BsaWI w'CCGG_w
Cuts at: 323 704 1409 1930 2608 3019 3166 3440 323
Size: 381 705 521 678 411 147 274 1103
Fragments arranged by size:
1103 705 678 521 411 381 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 571 601 571
Size: 30 4190
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 409 745 358
Size: 51 336 3833
Fragments arranged by size:
3833 336 51
BseYI C'CCAG_C
Cuts at: 1645 2403 3068 3476 1645
Size: 758 665 408 2389
Fragments arranged by size:
2389 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1762 1808 3038 104
Size: 1658 46 1230 1286
Fragments arranged by size:
1658 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 115 212 569 1016 1234 2012 2202 3062 115
Size: 97 357 447 218 778 190 860 1273
Fragments arranged by size:
1273 860 778 447 357 218 190 97
Bsp1286I G_dGCh'C
Cuts at: 115 212 569 1016 1090 1234 1845 1938 2012
Size: 97 357 447 74 144 611 93 74
Cuts at: 2012 2202 2264 3062 115
Size: 190 62 798 1273
Fragments arranged by size:
1273 798 611 447 357 190 144 97
93 74 74 62
BspEI T'CCGG_A
Cuts at: 704 704
Size: 4220
BspHI T'CATG_A
Cuts at: 629 2652 629
Size: 2023 2197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1270 1484 1786 2167 1270
Size: 214 302 381 3323
Fragments arranged by size:
3323 381 302 214
BsrBI CCG'CTC
Cuts at: 50 530 720 2512 2566 4050 4215 50
Size: 480 190 1792 54 1484 165 55
Fragments arranged by size:
1792 1484 480 190 165 55 54
BsrDI GCAATG_nn'
Cuts at: 637 936 2132 637
Size: 299 1196 2725
Fragments arranged by size:
2725 1196 299
BsrFI r'CCGG_y
Cuts at: 2218 2399 2218
Size: 181 4039
BsrGI T'GTAC_A
Cuts at: 314 314
Size: 4220
BssHII G'CGCG_C
Cuts at: 830 1031 830
Size: 201 4019
BssSI C'ACGA_G
Cuts at: 2491 3199 2491
Size: 708 3512
BstAPI GCAn_nnn'nTGC
Cuts at: 606 1179 1203 1597 606
Size: 573 24 394 3229
Fragments arranged by size:
3229 573 394 24
BstBI TT'CG_AA
Cuts at: 2581 2581
Size: 4220
BstYI r'GATC_y
Cuts at: 1 701 722 749 1245 1712 1735 2070 2316
Size: 700 21 27 496 467 23 335 246
Cuts at: 2316 2622 2634 2720 3772 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 700 496 467 449 335 306 246
86 27 23 21 12
BstZ17I GTA'TAC
Cuts at: 759 759
Size: 4220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 654 2170 2351 2514 654
Size: 1516 181 163 2360
Fragments arranged by size:
2360 1516 181 163
BtsI GCAGTG_nn'
Cuts at: 193 567 1035 1463 193
Size: 374 468 428 2950
Fragments arranged by size:
2950 468 428 374
DraI TTT'AAA
Cuts at: 1219 1219
Size: 4220
DraIII CAC_nnn'GTG
Cuts at: 384 467 384
Size: 83 4137
DrdI GACnn_nn'nnGTC
Cuts at: 688 1926 3270 688
Size: 1238 1344 1638
Fragments arranged by size:
1638 1344 1238
EaeI y'GGCC_r
Cuts at: 610 1805 1979 2370 2397 3742 610
Size: 1195 174 391 27 1345 1088
Fragments arranged by size:
1345 1195 1088 391 174 27
EagI C'GGCC_G
Cuts at: 1805 1805
Size: 4220
EarI CTCTTCn'nnn_
Cuts at: 304 2243 2453 304
Size: 1939 210 2071
Fragments arranged by size:
2071 1939 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2428 3170 3316 4030 2428
Size: 742 146 714 2618
Fragments arranged by size:
2618 742 714 146
EcoICRI GAG'CTC
Cuts at: 1232 1232
Size: 4220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 733 841 2045 2477 2830 3492 433
Size: 300 108 1204 432 353 662 1161
Fragments arranged by size:
1204 1161 662 432 353 300 108
EcoO109I rG'GnC_Cy
Cuts at: 1359 1359
Size: 4220
EcoRI G'AATT_C
Cuts at: 1224 1224
Size: 4220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 249 2239 249
Size: 1990 2230
FspI TGC'GCA
Cuts at: 958 2001 958
Size: 1043 3177
HaeII r_GCGC'y
Cuts at: 1654 1902 3132 3903 1654
Size: 248 1230 771 1971
Fragments arranged by size:
1971 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2062 2092 2094 2124 2062
Size: 30 2 30 4158
Fragments arranged by size:
4158 30 30 2
HincII GTy'rAC
Cuts at: 456 654 1259 456
Size: 198 605 3417
Fragments arranged by size:
3417 605 198
HindIII A'AGCT_T
Cuts at: 1135 1135
Size: 4220
KasI G'GCGC_C
Cuts at: 1650 1898 1650
Size: 248 3972
KpnI G_GTAC'C
Cuts at: 1240 1240
Size: 4220
MluI A'CGCG_T
Cuts at: 3653 3653
Size: 4220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2979 3163 2979
Size: 184 4036
MscI TGG'CCA
Cuts at: 1981 1981
Size: 4220
MslI CAynn'nnrTG
Cuts at: 247 424 862 979 2209 2336 247
Size: 177 438 117 1230 127 2131
Fragments arranged by size:
2131 1230 438 177 127 117
MspA1I CmG'CkG
Cuts at: 882 1107 1331 1645 2005 2789 3034 882
Size: 225 224 314 360 784 245 2068
Fragments arranged by size:
2068 784 360 314 245 225 224
NaeI GCC'GGC
Cuts at: 2401 2401
Size: 4220
NarI GG'CG_CC
Cuts at: 1651 1899 1651
Size: 248 3972
NdeI CA'TA_TG
Cuts at: 980 980
Size: 4220
NgoMIV G'CCGG_C
Cuts at: 2399 2399
Size: 4220
NspI r_CATG'y
Cuts at: 966 1173 1273 1773 3376 966
Size: 207 100 500 1603 1810
Fragments arranged by size:
1810 1603 500 207 100
PasI CC'CwG_GG
Cuts at: 905 905
Size: 4220
PciI A'CATG_T
Cuts at: 3372 3372
Size: 4220
PfoI T'CCnGG_A
Cuts at: 725 725
Size: 4220
PmeI GTTT'AAAC
Cuts at: 1219 1219
Size: 4220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2698 2666
Size: 32 4188
PstI C_TGCA'G
Cuts at: 1187 1267 1952 1187
Size: 80 685 3455
Fragments arranged by size:
3455 685 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4220
PvuII CAG'CTG
Cuts at: 1645 2005 1645
Size: 360 3860
RsrII CG'GwC_CG
Cuts at: 2415 2415
Size: 4220
SacI G_AGCT'C
Cuts at: 1234 1234
Size: 4220
SalI G'TCGA_C
Cuts at: 1257 1257
Size: 4220
SapI GCTCTTCn'nnn_
Cuts at: 2243 2453 2243
Size: 210 4010
SbfI CC_TGCA'GG
Cuts at: 1267 1267
Size: 4220
ScaI AGT'ACT
Cuts at: 514 3737 514
Size: 3223 997
SexAI A'CCwGG_T
Cuts at: 203 1211 203
Size: 1008 3212
SfcI C'TryA_G
Cuts at: 33 1183 1263 1948 2916 3107 33
Size: 1150 80 685 968 191 1146
Fragments arranged by size:
1150 1146 968 685 191 80
SfoI GGC'GCC
Cuts at: 1652 1900 1652
Size: 248 3972
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
SmaI CCC'GGG
Cuts at: 1242 1242
Size: 4220
SmlI C'TyrA_G
Cuts at: 991 1375 2290 3004 3266 991
Size: 384 915 714 262 1945
Fragments arranged by size:
1945 915 714 384 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4220
SphI G_CATG'C
Cuts at: 1173 1273 1773 1173
Size: 100 500 3620
Fragments arranged by size:
3620 500 100
StuI AGG'CCT
Cuts at: 119 269 119
Size: 150 4070
StyI C'CwwG_G
Cuts at: 872 1354 1708 872
Size: 482 354 3384
Fragments arranged by size:
3384 482 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1803 1936 2395 2604 1803
Size: 133 459 209 3419
Fragments arranged by size:
3419 459 209 133
TatI w'GTAC_w
Cuts at: 314 512 3735 314
Size: 198 3223 799
Fragments arranged by size:
3223 799 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 988 1020 3737 3769 988
Size: 32 2717 32 1439
Fragments arranged by size:
2717 1439 32 32
Tth111I GACn'n_nGTC
Cuts at: 451 2017 451
Size: 1566 2654
XbaI T'CTAG_A
Cuts at: 1251 1251
Size: 4220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1715 1715
Size: 4220
XmaI C'CCGG_G
Cuts at: 1240 1240
Size: 4220
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstYI BstZ17I BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmtI BpmI
BsgI BsiWI BsmI BsmBI BstEII BstXI Bsu36I BtgI
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI