Restriction map for
pF1KB3878
(Circular) (Six-base) MAPSORT of: pF1KB3878.seq Check: 334 from: 1 to: 4259
pF1KB3878 4259 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1169 1169
Size: 4259
AccI GT'mk_AC
Cuts at: 1297 1297
Size: 4259
Acc65I G'GTAC_C
Cuts at: 1275 1275
Size: 4259
AclI AA'CG_TT
Cuts at: 4099 4099
Size: 4259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 663 2084 2516 3263 3531 663
Size: 1421 432 747 268 1391
Fragments arranged by size:
1421 1391 747 432 268
AflIII A'CryG_T
Cuts at: 1611 2715 3692 1611
Size: 1104 977 2178
Fragments arranged by size:
2178 1104 977
AgeI A'CCGG_T
Cuts at: 2647 2647
Size: 4259
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 640 672 640
Size: 32 4227
AlwNI CAG_nnn'CTG
Cuts at: 1564 3131 1564
Size: 1567 2692
ApaI G_GGCC'C
Cuts at: 203 203
Size: 4259
ApaLI G'TGCA_C
Cuts at: 3029 3029
Size: 4259
ApoI r'AATT_y
Cuts at: 1263 3612 3868 1263
Size: 2349 256 1654
Fragments arranged by size:
2349 1654 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 190 202 1279 190
Size: 12 1077 3170
Fragments arranged by size:
3170 1077 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1039 1072 1039
Size: 33 4226
BamHI G'GATC_C
Cuts at: 1284 1284
Size: 4259
BanI G'GyrC_C
Cuts at: 1275 1689 1937 1972 1275
Size: 414 248 35 3562
Fragments arranged by size:
3562 414 248 35
BanII G_rGCy'C
Cuts at: 203 458 1273 2303 203
Size: 255 815 1030 2159
Fragments arranged by size:
2159 1030 815 255
BbeI G_GCGC'C
Cuts at: 1693 1941 1693
Size: 248 4011
BbsI GAAGACnn'nnnn_
Cuts at: 669 732 669
Size: 63 4196
BbvCI CC'TCA_GC
Cuts at: 248 634 248
Size: 386 3873
BciVI GTATCCnnnnn_n'
Cuts at: 880 2157 2918 4259 880
Size: 1277 761 1341 880
Fragments arranged by size:
1341 1277 880 761
BclI T'GATC_A
Cuts at: 1779 1779
Size: 4259
BglI GCCn_nnn'nGGC
Cuts at: 157 157
Size: 4259
BglII A'GATC_T
Cuts at: 1774 3811 1774
Size: 2037 2222
BlpI GC'TnA_GC
Cuts at: 640 727 1371 640
Size: 87 644 3528
Fragments arranged by size:
3528 644 87
Bme1580I G_kGCm'C
Cuts at: 203 1028 1884 1977 3033 203
Size: 825 856 93 1056 1429
Fragments arranged by size:
1429 1056 856 825 93
BmrI ACTGGGnnnn_n'
Cuts at: 206 1471 1552 1887 4031 206
Size: 1265 81 335 2144 434
Fragments arranged by size:
2144 1265 434 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 621 2626 621
Size: 2005 2254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 418 565 642 646 697 709 1165 281
Size: 137 147 77 4 51 12 456 3375
Fragments arranged by size:
3375 456 147 137 77 51 12 4
Bpu10I CC'TnA_GC
Cuts at: 248 634 248
Size: 386 3873
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1435 2314 2806 3104 751
Size: 684 879 492 298 1906
Fragments arranged by size:
1906 879 684 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 865 3963 865
Size: 3098 1161
BsaAI yAC'GTr
Cuts at: 1612 1612
Size: 4259
BsaBI GATnn'nnATC
Cuts at: 6 1773 3810 6
Size: 1767 2037 455
Fragments arranged by size:
2037 1767 455
BsaHI Gr'CG_yC
Cuts at: 737 1690 1938 2640 4136 737
Size: 953 248 702 1496 860
Fragments arranged by size:
1496 953 860 702 248
BsaWI w'CCGG_w
Cuts at: 1448 1969 2647 2921 3068 3479 1448
Size: 521 678 274 147 411 2228
Fragments arranged by size:
2228 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 640 670 640
Size: 30 4229
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 121 275 1125 121
Size: 154 850 3255
Fragments arranged by size:
3255 850 154
BseYI C'CCAG_C
Cuts at: 238 1684 2442 3019 3515 238
Size: 1446 758 577 496 982
Fragments arranged by size:
1446 982 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1801 1847 3055 104
Size: 1697 46 1208 1308
Fragments arranged by size:
1697 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 458 995 1273 2051 2241 3033 458
Size: 537 278 778 190 792 1684
Fragments arranged by size:
1684 792 778 537 278 190
BsmI GAATG_Cn'
Cuts at: 340 483 340
Size: 143 4116
Bsp1286I G_dGCh'C
Cuts at: 203 458 995 1028 1273 1884 1977 2051 2241
Size: 255 537 33 245 611 93 74 190
Cuts at: 2241 2303 3033 203
Size: 62 730 1429
Fragments arranged by size:
1429 730 611 537 255 245 190 93
74 62 33
BspEI T'CCGG_A
Cuts at: 3479 3479
Size: 4259
BspHI T'CATG_A
Cuts at: 1217 3435 1217
Size: 2218 2041
BspMI ACCTGCnnnn'nnnn_
Cuts at: 287 395 704 992 1169 1178 1309 1523 1825
Size: 108 309 288 177 9 131 214 302
Cuts at: 1825 2206 287
Size: 381 2340
Fragments arranged by size:
2340 381 309 302 288 214 177 131
108 9
BsrBI CCG'CTC
Cuts at: 50 2551 2605 4089 4254 50
Size: 2501 54 1484 165 55
Fragments arranged by size:
2501 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2171 2171
Size: 4259
BsrFI r'CCGG_y
Cuts at: 2257 2438 2647 2257
Size: 181 209 3869
Fragments arranged by size:
3869 209 181
BssSI C'ACGA_G
Cuts at: 2530 2888 2530
Size: 358 3901
BstAPI GCAn_nnn'nTGC
Cuts at: 1636 1636
Size: 4259
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstEII G'GTnAC_C
Cuts at: 113 113
Size: 4259
BstXI CCAn_nnnn'nTGG
Cuts at: 1209 1209
Size: 4259
BstYI r'GATC_y
Cuts at: 1 708 1284 1751 1774 2109 2355 3367 3453
Size: 707 576 467 23 335 246 1012 86
Cuts at: 3453 3465 3811 1
Size: 12 346 449
Fragments arranged by size:
1012 707 576 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 759 1244 759
Size: 485 3774
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 507 2209 2390 2553 507
Size: 1702 181 163 2213
Fragments arranged by size:
2213 1702 181 163
BtsI GCAGTG_nn'
Cuts at: 1026 1502 1026
Size: 476 3783
DraI TTT'AAA
Cuts at: 1258 1258
Size: 4259
DrdI GACnn_nn'nnGTC
Cuts at: 1965 2823 1965
Size: 858 3401
EaeI y'GGCC_r
Cuts at: 1844 2018 2409 2436 3781 1844
Size: 174 391 27 1345 2322
Fragments arranged by size:
2322 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1844 1844
Size: 4259
EarI CTCTTCn'nnn_
Cuts at: 590 2282 2492 590
Size: 1692 210 2357
Fragments arranged by size:
2357 1692 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1219 2467 2777 2923 4069 1219
Size: 1248 310 146 1146 1409
Fragments arranged by size:
1409 1248 1146 310 146
EcoICRI GAG'CTC
Cuts at: 456 1271 456
Size: 815 3444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 418 565 642 646 663 697 709 1165
Size: 137 147 77 4 17 34 12 456
Cuts at: 1165 2084 2516 3263 3531 281
Size: 919 432 747 268 1009
Fragments arranged by size:
1009 919 747 456 432 268 147 137
77 34 17 12 4
EcoNI CCTnn'n_nnAGG
Cuts at: 391 391
Size: 4259
EcoO109I rG'GnC_Cy
Cuts at: 301 396 600 825 1398 301
Size: 95 204 225 573 3162
Fragments arranged by size:
3162 573 225 204 95
EcoRI G'AATT_C
Cuts at: 1263 1263
Size: 4259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 984 2278 984
Size: 1294 2965
FspI TGC'GCA
Cuts at: 2040 2040
Size: 4259
HaeII r_GCGC'y
Cuts at: 1693 1941 2963 3942 1693
Size: 248 1022 979 2010
Fragments arranged by size:
2010 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 343 375 1120 1152 2101 2131 2133 2163 343
Size: 32 745 32 949 30 2 30 2439
Fragments arranged by size:
2439 949 745 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1298 1298
Size: 4259
KasI G'GCGC_C
Cuts at: 1689 1937 1689
Size: 248 4011
KpnI G_GTAC'C
Cuts at: 1279 1279
Size: 4259
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 572 1004 2930 3114 572
Size: 432 1926 184 1717
Fragments arranged by size:
1926 1717 432 184
MscI TGG'CCA
Cuts at: 2020 2020
Size: 4259
MslI CAynn'nnrTG
Cuts at: 2248 2375 2248
Size: 127 4132
MspA1I CmG'CkG
Cuts at: 633 1209 1370 1684 2044 3057 3302 633
Size: 576 161 314 360 1013 245 1590
Fragments arranged by size:
1590 1013 576 360 314 245 161
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 4259
NarI GG'CG_CC
Cuts at: 1690 1938 1690
Size: 248 4011
NcoI C'CATG_G
Cuts at: 759 759
Size: 4259
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 4259
NspI r_CATG'y
Cuts at: 1312 1812 2719 1312
Size: 500 907 2852
Fragments arranged by size:
2852 907 500
PasI CC'CwG_GG
Cuts at: 821 821
Size: 4259
PciI A'CATG_T
Cuts at: 2715 2715
Size: 4259
PfoI T'CCnGG_A
Cuts at: 704 704
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 640 672 3398 3430 640
Size: 32 2726 32 1469
Fragments arranged by size:
2726 1469 32 32
PpuMI rG'GwC_Cy
Cuts at: 396 600 825 396
Size: 204 225 3830
Fragments arranged by size:
3830 225 204
PspOMI G'GGCC_C
Cuts at: 199 199
Size: 4259
PstI C_TGCA'G
Cuts at: 1241 1306 1991 1241
Size: 65 685 3509
Fragments arranged by size:
3509 685 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4259
PvuII CAG'CTG
Cuts at: 633 1684 2044 633
Size: 1051 360 2848
Fragments arranged by size:
2848 1051 360
RsrII CG'GwC_CG
Cuts at: 2454 2454
Size: 4259
SacI G_AGCT'C
Cuts at: 458 1273 458
Size: 815 3444
SalI G'TCGA_C
Cuts at: 1296 1296
Size: 4259
SanDI GG'GwC_CC
Cuts at: 825 825
Size: 4259
SapI GCTCTTCn'nnn_
Cuts at: 2282 2492 2282
Size: 210 4049
SbfI CC_TGCA'GG
Cuts at: 1306 1306
Size: 4259
ScaI AGT'ACT
Cuts at: 3776 3776
Size: 4259
SfcI C'TryA_G
Cuts at: 33 1237 1302 1987 2980 3171 33
Size: 1204 65 685 993 191 1121
Fragments arranged by size:
1204 1121 993 685 191 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 157 157
Size: 4259
SfoI GGC'GCC
Cuts at: 1691 1939 1691
Size: 248 4011
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SmaI CCC'GGG
Cuts at: 192 204 1281 192
Size: 12 1077 3170
Fragments arranged by size:
3170 1077 12
SmlI C'TyrA_G
Cuts at: 766 1414 2329 2821 3083 766
Size: 648 915 492 262 1942
Fragments arranged by size:
1942 915 648 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 1312 1812 1312
Size: 500 3759
SrfI GCCC'GGGC
Cuts at: 204 204
Size: 4259
StuI AGG'CCT
Cuts at: 488 488
Size: 4259
StyI C'CwwG_G
Cuts at: 162 759 1393 1747 162
Size: 597 634 354 2674
Fragments arranged by size:
2674 634 597 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 252 1842 1975 2434 2643 252
Size: 1590 133 459 209 1868
Fragments arranged by size:
1868 1590 459 209 133
TatI w'GTAC_w
Cuts at: 3774 3774
Size: 4259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1005 1037 3776 3808 1005
Size: 32 2739 32 1456
Fragments arranged by size:
2739 1456 32 32
Tth111I GACn'n_nGTC
Cuts at: 736 2056 736
Size: 1320 2939
XbaI T'CTAG_A
Cuts at: 1290 1290
Size: 4259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1113 1754 1113
Size: 641 3618
XmaI C'CCGG_G
Cuts at: 190 202 1279 190
Size: 12 1077 3170
Fragments arranged by size:
3170 1077 12
XmnI GAAnn'nnTTC
Cuts at: 442 442
Size: 4259
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SgrAI SnaBI SspI SwaI XhoI ZraI