Restriction map for
pF1KB3867
(Circular) (Six-base) MAPSORT of: pF1KB3867.seq Check: 398 from: 1 to: 4175
pF1KB3867 4175 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 713 764 713
Size: 51 4124
AccI GT'mk_AC
Cuts at: 326 1213 326
Size: 887 3288
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 681 2000 2432 3179 3447 681
Size: 1319 432 747 268 1409
Fragments arranged by size:
1409 1319 747 432 268
AflIII A'CryG_T
Cuts at: 1527 2631 3608 1527
Size: 1104 977 2094
Fragments arranged by size:
2094 1104 977
AgeI A'CCGG_T
Cuts at: 533 2563 533
Size: 2030 2145
AleI CACnn'nnGTG
Cuts at: 253 253
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 348 1480 3047 348
Size: 1132 1567 1476
Fragments arranged by size:
1567 1476 1132
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 1179 3528 3784 1179
Size: 2349 256 1570
Fragments arranged by size:
2349 1570 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 145 223 539 1195 145
Size: 78 316 656 3125
Fragments arranged by size:
3125 656 316 78
BamHI G'GATC_C
Cuts at: 743 1200 743
Size: 457 3718
BanI G'GyrC_C
Cuts at: 1191 1605 1853 1888 1191
Size: 414 248 35 3478
Fragments arranged by size:
3478 414 248 35
BanII G_rGCy'C
Cuts at: 464 1189 2219 464
Size: 725 1030 2420
Fragments arranged by size:
2420 1030 725
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BbsI GAAGACnn'nnnn_
Cuts at: 305 458 824 1007 305
Size: 153 366 183 3473
Fragments arranged by size:
3473 366 183 153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 819 853 819
Size: 34 4141
BciVI GTATCCnnnnn_n'
Cuts at: 2073 2834 4175 2073
Size: 761 1341 2073
Fragments arranged by size:
2073 1341 761
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BglII A'GATC_T
Cuts at: 924 1690 3727 924
Size: 766 2037 1372
Fragments arranged by size:
2037 1372 766
BlpI GC'TnA_GC
Cuts at: 790 1287 790
Size: 497 3678
Bme1580I G_kGCm'C
Cuts at: 1800 1893 2949 1800
Size: 93 1056 3026
Fragments arranged by size:
3026 1056 93
BmgBI CAC'GTC
Cuts at: 207 303 207
Size: 96 4079
BmrI ACTGGGnnnn_n'
Cuts at: 1387 1468 1803 3947 1387
Size: 81 335 2144 1615
Fragments arranged by size:
2144 1615 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 160 483 2542 160
Size: 323 2059 1793
Fragments arranged by size:
2059 1793 323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 251
Size: 4175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 2230 2722 3020 1351
Size: 879 492 298 2506
Fragments arranged by size:
2506 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3879 3879
Size: 4175
BsaAI yAC'GTr
Cuts at: 1528 1528
Size: 4175
BsaBI GATnn'nnATC
Cuts at: 6 268 1689 3726 6
Size: 262 1421 2037 455
Fragments arranged by size:
2037 1421 455 262
BsaHI Gr'CG_yC
Cuts at: 416 710 761 1118 1606 1854 2556 4052 416
Size: 294 51 357 488 248 702 1496 539
Fragments arranged by size:
1496 702 539 488 357 294 248 51
BsaWI w'CCGG_w
Cuts at: 292 533 1364 1885 2563 2837 2984 3395 292
Size: 241 831 521 678 274 147 411 1072
Fragments arranged by size:
1072 831 678 521 411 274 241 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 287 317 287
Size: 30 4145
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 145
Size: 4175
BseYI C'CCAG_C
Cuts at: 1600 2358 2935 3431 1600
Size: 758 577 496 2344
Fragments arranged by size:
2344 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1717 1763 2971 104
Size: 1613 46 1208 1308
Fragments arranged by size:
1613 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 812 1189 1967 2157 2949 812
Size: 377 778 190 792 2038
Fragments arranged by size:
2038 792 778 377 190
Bsp1286I G_dGCh'C
Cuts at: 464 812 1189 1800 1893 1967 2157 2219 2949
Size: 348 377 611 93 74 190 62 730
Cuts at: 2949 464
Size: 1690
Fragments arranged by size:
1690 730 611 377 348 190 93 74
62
BspEI T'CCGG_A
Cuts at: 292 3395 292
Size: 3103 1072
BspHI T'CATG_A
Cuts at: 3351 3351
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1225 1439 1741 2122 1225
Size: 214 302 381 3278
Fragments arranged by size:
3278 381 302 214
BsrBI CCG'CTC
Cuts at: 50 150 1046 2467 2521 4005 4170 50
Size: 100 896 1421 54 1484 165 55
Fragments arranged by size:
1484 1421 896 165 100 55 54
BsrDI GCAATG_nn'
Cuts at: 747 1136 2087 747
Size: 389 951 2835
Fragments arranged by size:
2835 951 389
BsrFI r'CCGG_y
Cuts at: 533 2173 2354 2563 533
Size: 1640 181 209 2145
Fragments arranged by size:
2145 1640 209 181
BssSI C'ACGA_G
Cuts at: 841 2446 2804 841
Size: 1605 358 2212
Fragments arranged by size:
2212 1605 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1552 1552
Size: 4175
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstXI CCAn_nnnn'nTGG
Cuts at: 283 283
Size: 4175
BstYI r'GATC_y
Cuts at: 1 743 924 1200 1667 1690 2025 2271 3283
Size: 742 181 276 467 23 335 246 1012
Cuts at: 3283 3369 3381 3727 1
Size: 86 12 346 449
Fragments arranged by size:
1012 742 467 449 346 335 276 246
181 86 23 12
BtgI C'CryG_G
Cuts at: 107 368 107
Size: 261 3914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2125 2306 2469 2125
Size: 181 163 3831
Fragments arranged by size:
3831 181 163
BtsI GCAGTG_nn'
Cuts at: 1418 1418
Size: 4175
ClaI AT'CG_AT
Cuts at: 890 890
Size: 4175
DraI TTT'AAA
Cuts at: 1174 1174
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 767 1760 1934 2325 2352 3697 767
Size: 993 174 391 27 1345 1245
Fragments arranged by size:
1345 1245 993 391 174 27
EagI C'GGCC_G
Cuts at: 1760 1760
Size: 4175
EarI CTCTTCn'nnn_
Cuts at: 862 913 2198 2408 862
Size: 51 1285 210 2629
Fragments arranged by size:
2629 1285 210 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2383 2693 2839 3985 2383
Size: 310 146 1146 2573
Fragments arranged by size:
2573 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 681 2000 2432 3179 3447 251
Size: 430 1319 432 747 268 979
Fragments arranged by size:
1319 979 747 432 430 268
EcoNI CCTnn'n_nnAGG
Cuts at: 178 178
Size: 4175
EcoO109I rG'GnC_Cy
Cuts at: 985 1314 985
Size: 329 3846
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 4175
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 197 1609 1857 2879 3858 197
Size: 1412 248 1022 979 514
Fragments arranged by size:
1412 1022 979 514 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 2017 2047 2049 2079 274
Size: 32 1711 30 2 30 2370
Fragments arranged by size:
2370 1711 32 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 424 693 2846 3030 424
Size: 269 2153 184 1569
Fragments arranged by size:
2153 1569 269 184
MscI TGG'CCA
Cuts at: 1936 1936
Size: 4175
MslI CAynn'nnrTG
Cuts at: 253 281 1105 2164 2291 253
Size: 28 824 1059 127 2137
Fragments arranged by size:
2137 1059 824 127 28
MspA1I CmG'CkG
Cuts at: 794 1286 1600 1960 2973 3218 794
Size: 492 314 360 1013 245 1751
Fragments arranged by size:
1751 1013 492 360 314 245
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 4175
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NcoI C'CATG_G
Cuts at: 107 368 107
Size: 261 3914
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 4175
NspI r_CATG'y
Cuts at: 840 1228 1728 2635 840
Size: 388 500 907 2380
Fragments arranged by size:
2380 907 500 388
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PflMI CCAn_nnn'nTGG
Cuts at: 348 348
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4143
PspXI vC'TCGA_Gb
Cuts at: 145 145
Size: 4175
PstI C_TGCA'G
Cuts at: 474 1222 1907 474
Size: 748 685 2742
Fragments arranged by size:
2742 748 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 1600 1960 1600
Size: 360 3815
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 862 2198 2408 862
Size: 1336 210 2629
Fragments arranged by size:
2629 1336 210
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SexAI A'CCwGG_T
Cuts at: 803 803
Size: 4175
SfcI C'TryA_G
Cuts at: 33 327 470 1218 1903 2896 3087 33
Size: 294 143 748 685 993 191 1121
Fragments arranged by size:
1121 993 748 685 294 191 143
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SmaI CCC'GGG
Cuts at: 541 1197 541
Size: 656 3519
SmlI C'TyrA_G
Cuts at: 145 1330 2245 2737 2999 145
Size: 1185 915 492 262 1321
Fragments arranged by size:
1321 1185 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 840 1228 1728 840
Size: 388 500 3287
Fragments arranged by size:
3287 500 388
StyI C'CwwG_G
Cuts at: 107 368 1309 1663 107
Size: 261 941 354 2619
Fragments arranged by size:
2619 941 354 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1065 1758 1891 2350 2559 1065
Size: 693 133 459 209 2681
Fragments arranged by size:
2681 693 459 209 133
TatI w'GTAC_w
Cuts at: 115 311 3690 115
Size: 196 3379 600
Fragments arranged by size:
3379 600 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 287 319 3692 3724 287
Size: 32 3373 32 738
Fragments arranged by size:
3373 738 32 32
Tth111I GACn'n_nGTC
Cuts at: 1120 1972 1120
Size: 852 3323
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 375 573 1670 375
Size: 198 1097 2880
Fragments arranged by size:
2880 1097 198
XhoI C'TCGA_G
Cuts at: 145 145
Size: 4175
XmaI C'CCGG_G
Cuts at: 539 1195 539
Size: 656 3519
ZraI GAC'GTC
Cuts at: 711 762 711
Size: 51 4124
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI