Restriction map for
pF1KB3853
(Circular) (Six-base) MAPSORT of: pF1KB3853.seq Check: 7393 from: 1 to: 3749
pF1KB3853 3749 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 311 787 311
Size: 476 3273
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 722 1574 2006 2753 3021 555
Size: 167 852 432 747 268 1283
Fragments arranged by size:
1283 852 747 432 268 167
AflIII A'CryG_T
Cuts at: 1101 2205 3182 1101
Size: 1104 977 1668
Fragments arranged by size:
1668 1104 977
AgeI A'CCGG_T
Cuts at: 306 2137 306
Size: 1831 1918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 489 489
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 409 477 1054 2621 409
Size: 68 577 1567 1537
Fragments arranged by size:
1567 1537 577 68
ApaI G_GGCC'C
Cuts at: 485 485
Size: 3749
ApaLI G'TGCA_C
Cuts at: 423 2519 423
Size: 2096 1653
ApoI r'AATT_y
Cuts at: 229 544 753 3102 3358 229
Size: 315 209 2349 256 620
Fragments arranged by size:
2349 620 315 256 209
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 769 769
Size: 3749
BamHI G'GATC_C
Cuts at: 268 774 268
Size: 506 3243
BanI G'GyrC_C
Cuts at: 765 1179 1427 1462 765
Size: 414 248 35 3052
Fragments arranged by size:
3052 414 248 35
BanII G_rGCy'C
Cuts at: 485 763 1793 485
Size: 278 1030 2441
Fragments arranged by size:
2441 1030 278
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BbsI GAAGACnn'nnnn_
Cuts at: 447 519 728 447
Size: 72 209 3468
Fragments arranged by size:
3468 209 72
BbvCI CC'TCA_GC
Cuts at: 259 259
Size: 3749
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglII A'GATC_T
Cuts at: 1264 3301 1264
Size: 2037 1712
BlpI GC'TnA_GC
Cuts at: 861 861
Size: 3749
Bme1580I G_kGCm'C
Cuts at: 427 485 1374 1467 2523 427
Size: 58 889 93 1056 1653
Fragments arranged by size:
1653 1056 889 93 58
BmrI ACTGGGnnnn_n'
Cuts at: 291 465 961 1042 1377 3521 291
Size: 174 496 81 335 2144 519
Fragments arranged by size:
2144 519 496 335 174 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 517 2116 517
Size: 1599 2150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 743 683
Size: 60 3689
Bpu10I CC'TnA_GC
Cuts at: 259 259
Size: 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 925 1804 2296 2594 185
Size: 740 879 492 298 1340
Fragments arranged by size:
1340 879 740 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3453 3453
Size: 3749
BsaAI yAC'GTr
Cuts at: 1102 1102
Size: 3749
BsaBI GATnn'nnATC
Cuts at: 6 439 1263 3300 6
Size: 433 824 2037 455
Fragments arranged by size:
2037 824 455 433
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 306 938 1459 2137 2411 2558 2969 306
Size: 632 521 678 274 147 411 1086
Fragments arranged by size:
1086 678 632 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 712 247
Size: 465 3284
BseYI C'CCAG_C
Cuts at: 584 1174 1932 2509 3005 584
Size: 590 758 577 496 1328
Fragments arranged by size:
1328 758 590 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 382
Size: 3749
BsiEI CG_ry'CG
Cuts at: 104 311 1291 1337 2545 104
Size: 207 980 46 1208 1308
Fragments arranged by size:
1308 1208 980 207 46
BsiHKAI G_wGCw'C
Cuts at: 427 763 1541 1731 2523 427
Size: 336 778 190 792 1653
Fragments arranged by size:
1653 792 778 336 190
BsmBI CGTCTCn'nnnn_
Cuts at: 631 631
Size: 3749
Bsp1286I G_dGCh'C
Cuts at: 427 485 763 1374 1467 1541 1731 1793 2523
Size: 58 278 611 93 74 190 62 730
Cuts at: 2523 427
Size: 1653
Fragments arranged by size:
1653 730 611 278 190 93 74 62
58
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 2925 2925
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 799 1013 1315 1696 799
Size: 214 302 381 2852
Fragments arranged by size:
2852 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2041 2095 3579 3744 50
Size: 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 347 1661 347
Size: 1314 2435
BsrFI r'CCGG_y
Cuts at: 306 1747 1928 2137 306
Size: 1441 181 209 1918
Fragments arranged by size:
1918 1441 209 181
BssSI C'ACGA_G
Cuts at: 2020 2378 2020
Size: 358 3391
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 1126
Size: 3749
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstYI r'GATC_y
Cuts at: 1 268 774 1241 1264 1599 1845 2857 2943
Size: 267 506 467 23 335 246 1012 86
Cuts at: 2943 2955 3301 1
Size: 12 346 449
Fragments arranged by size:
1012 506 467 449 346 335 267 246
86 23 12
BtgI C'CryG_G
Cuts at: 356 416 356
Size: 60 3689
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 426 447 1699 1880 2043 426
Size: 21 1252 181 163 2132
Fragments arranged by size:
2132 1252 181 163 21
BtsI GCAGTG_nn'
Cuts at: 370 410 992 370
Size: 40 582 3127
Fragments arranged by size:
3127 582 40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 267 302 267
Size: 35 3714
DraI TTT'AAA
Cuts at: 561 748 561
Size: 187 3562
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 236 372 387 413 1334 1508 1899 1926 3271
Size: 136 15 26 921 174 391 27 1345
Cuts at: 3271 236
Size: 714
Fragments arranged by size:
1345 921 714 391 174 136 27 26
15
EagI C'GGCC_G
Cuts at: 1334 1334
Size: 3749
EarI CTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2267 2413 3559 1957
Size: 310 146 1146 2147
Fragments arranged by size:
2147 1146 310 146
EcoICRI GAG'CTC
Cuts at: 761 761
Size: 3749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 683 722 743 1574 2006 2753 3021 555
Size: 128 39 21 831 432 747 268 1283
Fragments arranged by size:
1283 831 747 432 268 128 39 21
EcoO109I rG'GnC_Cy
Cuts at: 481 888 481
Size: 407 3342
EcoRI G'AATT_C
Cuts at: 544 753 544
Size: 209 3540
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 471 1768 471
Size: 1297 2452
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 1183 1431 2453 3432 1183
Size: 248 1022 979 1500
Fragments arranged by size:
1500 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 155 187 260 292 718 750 1591 1621 1623
Size: 32 73 32 426 32 841 30 2
Cuts at: 1623 1653 155
Size: 30 2251
Fragments arranged by size:
2251 841 426 73 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 312 687 788 312
Size: 375 101 3273
Fragments arranged by size:
3273 375 101
HindIII A'AGCT_T
Cuts at: 203 203
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2604 2420
Size: 184 3565
MscI TGG'CCA
Cuts at: 238 374 389 415 1510 238
Size: 136 15 26 1095 2477
Fragments arranged by size:
2477 1095 136 26 15
MslI CAynn'nnrTG
Cuts at: 175 610 1738 1865 175
Size: 435 1128 127 2059
Fragments arranged by size:
2059 1128 435 127
MspA1I CmG'CkG
Cuts at: 226 383 860 1174 1534 2547 2792 226
Size: 157 477 314 360 1013 245 1183
Fragments arranged by size:
1183 1013 477 360 314 245 157
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NcoI C'CATG_G
Cuts at: 356 416 356
Size: 60 3689
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NspI r_CATG'y
Cuts at: 676 802 1302 2209 676
Size: 126 500 907 2216
Fragments arranged by size:
2216 907 500 126
PciI A'CATG_T
Cuts at: 2205 2205
Size: 3749
PflMI CCAn_nnn'nTGG
Cuts at: 281 477 281
Size: 196 3553
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 3717
PspOMI G'GGCC_C
Cuts at: 481 481
Size: 3749
PstI C_TGCA'G
Cuts at: 405 796 1481 405
Size: 391 685 2673
Fragments arranged by size:
2673 685 391
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 1174 1534 1174
Size: 360 3389
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 763 763
Size: 3749
SalI G'TCGA_C
Cuts at: 310 786 310
Size: 476 3273
SapI GCTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SfcI C'TryA_G
Cuts at: 33 401 792 1477 2470 2661 33
Size: 368 391 685 993 191 1121
Fragments arranged by size:
1121 993 685 391 368 191
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 200 904 1819 2311 2573 200
Size: 704 915 492 262 1376
Fragments arranged by size:
1376 915 704 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StyI C'CwwG_G
Cuts at: 356 416 883 1237 356
Size: 60 467 354 2868
Fragments arranged by size:
2868 467 354 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1332 1465 1924 2133 1332
Size: 133 459 209 2948
Fragments arranged by size:
2948 459 209 133
TatI w'GTAC_w
Cuts at: 3264 3264
Size: 3749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 3717
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 382 1244 382
Size: 862 2887
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI