Restriction map for
pF1KB3845
(Circular) (Six-base) MAPSORT of: pF1KB3845.seq Check: 54 from: 1 to: 4163
pF1KB3845 4163 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1988 2420 2773 3435 589
Size: 1399 432 353 662 1317
Fragments arranged by size:
1399 1317 662 432 353
AflIII A'CryG_T
Cuts at: 1515 3315 3596 1515
Size: 1800 281 2082
Fragments arranged by size:
2082 1800 281
AgeI A'CCGG_T
Cuts at: 280 280
Size: 4163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 664 696 664
Size: 32 4131
AlwNI CAG_nnn'CTG
Cuts at: 1468 2906 1468
Size: 1438 2725
ApaLI G'TGCA_C
Cuts at: 3001 3001
Size: 4163
ApoI r'AATT_y
Cuts at: 763 808 826 1167 3516 3772 763
Size: 45 18 341 2349 256 1154
Fragments arranged by size:
2349 1154 341 256 45 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1183 1183
Size: 4163
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 159 186 1179 1593 1841 1876 159
Size: 27 993 414 248 35 2446
Fragments arranged by size:
2446 993 414 248 35 27
BanII G_rGCy'C
Cuts at: 122 170 530 1177 2207 122
Size: 48 360 647 1030 2078
Fragments arranged by size:
2078 1030 647 360 48
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BbsI GAAGACnn'nnnn_
Cuts at: 274 283 274
Size: 9 4154
BbvCI CC'TCA_GC
Cuts at: 694 694
Size: 4163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 760 794 760
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 3117 4163 2061
Size: 1056 1046 2061
Fragments arranged by size:
2061 1056 1046
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 191 1788 1881 3005 191
Size: 1597 93 1124 1349
Fragments arranged by size:
1597 1349 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 331 1150 1375 1456 1791 3935 331
Size: 819 225 81 335 2144 559
Fragments arranged by size:
2144 819 559 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 769 1018 363
Size: 406 249 3508
Fragments arranged by size:
3508 406 249
Bpu10I CC'TnA_GC
Cuts at: 164 176 694 728 164
Size: 12 518 34 3599
Fragments arranged by size:
3599 518 34 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1027 1339 2218 2932 3230 697
Size: 330 312 879 714 298 1630
Fragments arranged by size:
1630 879 714 330 312 298
BsaI GGTCTCn'nnnn_
Cuts at: 121 491 3867 121
Size: 370 3376 417
Fragments arranged by size:
3376 417 370
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 1594 1842 2544 4040 1594
Size: 248 702 1496 1717
Fragments arranged by size:
1717 1496 702 248
BsaWI w'CCGG_w
Cuts at: 280 1116 1352 1873 2551 2962 3109 3383 280
Size: 836 236 521 678 411 147 274 1060
Fragments arranged by size:
1060 836 678 521 411 274 236 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 742 772 742
Size: 30 4133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 409 589 766 409
Size: 180 177 3806
Fragments arranged by size:
3806 180 177
BseYI C'CCAG_C
Cuts at: 222 521 1588 2346 3011 3419 222
Size: 299 1067 758 665 408 966
Fragments arranged by size:
1067 966 758 665 408 299
BsiEI CG_ry'CG
Cuts at: 104 945 1705 1751 2981 104
Size: 841 760 46 1230 1286
Fragments arranged by size:
1286 1230 841 760 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 3005 1177
Size: 778 190 860 2335
Fragments arranged by size:
2335 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 122 170 191 530 1177 1788 1881 1955 2145
Size: 48 21 339 647 611 93 74 190
Cuts at: 2145 2207 3005 122
Size: 62 798 1280
Fragments arranged by size:
1280 798 647 611 339 190 93 74
62 48 21
BspHI T'CATG_A
Cuts at: 683 2595 683
Size: 1912 2251
BspMI ACCTGCnnnn'nnnn_
Cuts at: 227 1213 1427 1729 2110 227
Size: 986 214 302 381 2280
Fragments arranged by size:
2280 986 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 722 2075 722
Size: 1353 2810
BsrFI r'CCGG_y
Cuts at: 280 2161 2342 280
Size: 1881 181 2101
Fragments arranged by size:
2101 1881 181
BssSI C'ACGA_G
Cuts at: 614 2434 3142 614
Size: 1820 708 1635
Fragments arranged by size:
1820 1635 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstXI CCAn_nnnn'nTGG
Cuts at: 222 414 747 925 222
Size: 192 333 178 3460
Fragments arranged by size:
3460 333 192 178
BstYI r'GATC_y
Cuts at: 1 401 672 1188 1655 1678 2013 2259 2565
Size: 400 271 516 467 23 335 246 306
Cuts at: 2565 2577 2663 3715 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 516 467 449 400 335 306 271
246 86 23 12
BtgI C'CryG_G
Cuts at: 955 955
Size: 4163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 570 2113 2294 2457 570
Size: 1543 181 163 2276
Fragments arranged by size:
2276 1543 181 163
BtsI GCAGTG_nn'
Cuts at: 621 1406 621
Size: 785 3378
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 616 1869 3213 616
Size: 1253 1344 1566
Fragments arranged by size:
1566 1344 1253
EaeI y'GGCC_r
Cuts at: 309 942 1748 1922 2313 2340 3685 309
Size: 633 806 174 391 27 1345 787
Fragments arranged by size:
1345 806 787 633 391 174 27
EagI C'GGCC_G
Cuts at: 942 1748 942
Size: 806 3357
EarI CTCTTCn'nnn_
Cuts at: 355 2186 2396 355
Size: 1831 210 2122
Fragments arranged by size:
2122 1831 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 3113 3259 3973 2371
Size: 742 146 714 2561
Fragments arranged by size:
2561 742 714 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 589 769 1018 1988 2420 2773 3435 363
Size: 226 180 249 970 432 353 662 1091
Fragments arranged by size:
1091 970 662 432 353 249 226 180
EcoNI CCTnn'n_nnAGG
Cuts at: 571 697 571
Size: 126 4037
EcoO109I rG'GnC_Cy
Cuts at: 936 1025 1038 1302 936
Size: 89 13 264 3797
Fragments arranged by size:
3797 264 89 13
EcoRI G'AATT_C
Cuts at: 763 808 1167 763
Size: 45 359 3759
Fragments arranged by size:
3759 359 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 262 1944 262
Size: 1682 2481
HaeII r_GCGC'y
Cuts at: 1597 1845 3075 3846 1597
Size: 248 1230 771 1914
Fragments arranged by size:
1914 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 488 520 742 774 1001 1033 2005 2035 2037
Size: 32 222 32 227 32 972 30 2
Cuts at: 2037 2067 488
Size: 30 2584
Fragments arranged by size:
2584 972 227 222 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 342 582 1202 342
Size: 240 620 3303
Fragments arranged by size:
3303 620 240
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 239 2922 3106 239
Size: 2683 184 1296
Fragments arranged by size:
2683 1296 184
MscI TGG'CCA
Cuts at: 311 1924 311
Size: 1613 2550
MslI CAynn'nnrTG
Cuts at: 970 2152 2279 970
Size: 1182 127 2854
Fragments arranged by size:
2854 1182 127
MspA1I CmG'CkG
Cuts at: 184 383 1274 1588 1948 2732 2977 184
Size: 199 891 314 360 784 245 1370
Fragments arranged by size:
1370 891 784 360 314 245 199
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 955 955
Size: 4163
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 439 885 1216 1716 3319 439
Size: 446 331 500 1603 1283
Fragments arranged by size:
1603 1283 500 446 331
PciI A'CATG_T
Cuts at: 3315 3315
Size: 4163
PflMI CCAn_nnn'nTGG
Cuts at: 493 493
Size: 4163
PfoI T'CCnGG_A
Cuts at: 1034 1034
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 4131
PpuMI rG'GwC_Cy
Cuts at: 936 1025 1038 936
Size: 89 13 4061
Fragments arranged by size:
4061 89 13
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 547 579 547
Size: 32 4131
PstI C_TGCA'G
Cuts at: 1210 1895 1210
Size: 685 3478
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 383 1588 1948 383
Size: 1205 360 2598
Fragments arranged by size:
2598 1205 360
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 355 2186 2396 355
Size: 1831 210 2122
Fragments arranged by size:
2122 1831 210
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 1206 1891 2859 3050 33
Size: 1173 685 968 191 1146
Fragments arranged by size:
1173 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 676 1042 1318 2233 2947 3209 676
Size: 366 276 915 714 262 1630
Fragments arranged by size:
1630 915 714 366 276 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 885 1216 1716 885
Size: 331 500 3332
Fragments arranged by size:
3332 500 331
StuI AGG'CCT
Cuts at: 174 376 1109 174
Size: 202 733 3228
Fragments arranged by size:
3228 733 202
StyI C'CwwG_G
Cuts at: 955 1297 1651 955
Size: 342 354 3467
Fragments arranged by size:
3467 354 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 1879 2338 2547 1746
Size: 133 459 209 3362
Fragments arranged by size:
3362 459 209 133
TatI w'GTAC_w
Cuts at: 539 3678 539
Size: 3139 1024
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1046 1078 3680 3712 1046
Size: 32 2602 32 1497
Fragments arranged by size:
2602 1497 32 32
Tth111I GACn'n_nGTC
Cuts at: 1015 1960 1015
Size: 945 3218
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 153 1658 153
Size: 1505 2658
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI