Restriction map for
pF1KB3821
(Circular) (Six-base) MAPSORT of: pF1KB3821.seq Check: 4969 from: 1 to: 4382
pF1KB3821 4382 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 2639 3386 3654 2207
Size: 432 747 268 2935
Fragments arranged by size:
2935 747 432 268
AflIII A'CryG_T
Cuts at: 1734 2838 3815 1734
Size: 1104 977 2301
Fragments arranged by size:
2301 1104 977
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 188 1374 1687 3254 188
Size: 1186 313 1567 1316
Fragments arranged by size:
1567 1316 1186 313
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 1386 3735 3991 1386
Size: 2349 256 1777
Fragments arranged by size:
2349 1777 256
AseI AT'TA_AT
Cuts at: 20 322 20
Size: 302 4080
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 1402 1402
Size: 4382
BamHI G'GATC_C
Cuts at: 493 1407 493
Size: 914 3468
BanI G'GyrC_C
Cuts at: 429 1398 1812 2060 2095 429
Size: 969 414 248 35 2716
Fragments arranged by size:
2716 969 414 248 35
BanII G_rGCy'C
Cuts at: 1396 2426 1396
Size: 1030 3352
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4348
BciVI GTATCCnnnnn_n'
Cuts at: 689 2280 3041 4382 689
Size: 1591 761 1341 689
Fragments arranged by size:
1591 1341 761 689
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 2007 2100 3156 2007
Size: 93 1056 3233
Fragments arranged by size:
3233 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1594 1675 2010 4154 1594
Size: 81 335 2144 1822
Fragments arranged by size:
2144 1822 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 1000 210
Size: 790 3592
Bpu10I CC'TnA_GC
Cuts at: 1309 1309
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 877 1151 1558 2437 2929 3227 266
Size: 611 274 407 879 492 298 1421
Fragments arranged by size:
1421 879 611 492 407 298 274
BsaI GGTCTCn'nnnn_
Cuts at: 389 4086 389
Size: 3697 685
BsaAI yAC'GTr
Cuts at: 1735 1735
Size: 4382
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 155 1813 2061 2763 4259 155
Size: 1658 248 702 1496 278
Fragments arranged by size:
1658 1496 702 278 248
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 183 213 679 709 785 815 183
Size: 30 466 30 76 30 3750
Fragments arranged by size:
3750 466 76 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 793 793
Size: 4382
BseYI C'CCAG_C
Cuts at: 257 1807 2565 3142 3638 257
Size: 1550 758 577 496 1001
Fragments arranged by size:
1550 1001 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 245
Size: 4382
BsiEI CG_ry'CG
Cuts at: 104 239 1924 1970 3178 104
Size: 135 1685 46 1208 1308
Fragments arranged by size:
1685 1308 1208 135 46
BsiHKAI G_wGCw'C
Cuts at: 775 1396 2174 2364 3156 775
Size: 621 778 190 792 2001
Fragments arranged by size:
2001 792 778 621 190
BsmI GAATG_Cn'
Cuts at: 1263 1263
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 775 1396 2007 2100 2174 2364 2426 3156 775
Size: 621 611 93 74 190 62 730 2001
Fragments arranged by size:
2001 730 621 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 401 3558 401
Size: 3157 1225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1432 1646 1948 2329 1432
Size: 214 302 381 3485
Fragments arranged by size:
3485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1320 2674 2728 4212 4377 50
Size: 1270 1354 54 1484 165 55
Fragments arranged by size:
1484 1354 1270 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2294 2294
Size: 4382
BsrFI r'CCGG_y
Cuts at: 2380 2561 2770 2380
Size: 181 209 3992
Fragments arranged by size:
3992 209 181
BssHII G'CGCG_C
Cuts at: 144 144
Size: 4382
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 212 212
Size: 4382
BstYI r'GATC_y
Cuts at: 1 493 1407 1874 1897 2232 2478 3490 3576
Size: 492 914 467 23 335 246 1012 86
Cuts at: 3576 3588 3934 1
Size: 12 346 449
Fragments arranged by size:
1012 914 492 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 938 938
Size: 4382
BtgI C'CryG_G
Cuts at: 149 205 149
Size: 56 4326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 423 2332 2513 2676 423
Size: 1909 181 163 2129
Fragments arranged by size:
2129 1909 181 163
BtsI GCAGTG_nn'
Cuts at: 898 1625 898
Size: 727 3655
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1167 1202 1167
Size: 35 4347
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 236 1250 1967 2141 2532 2559 3904 236
Size: 1014 717 174 391 27 1345 714
Fragments arranged by size:
1345 1014 717 714 391 174 27
EagI C'GGCC_G
Cuts at: 236 1967 236
Size: 1731 2651
EarI CTCTTCn'nnn_
Cuts at: 292 388 626 856 1200 2405 2615 292
Size: 96 238 230 344 1205 210 2059
Fragments arranged by size:
2059 1205 344 238 230 210 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 506 2590 2900 3046 4192 506
Size: 2084 310 146 1146 696
Fragments arranged by size:
2084 1146 696 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 1000 2207 2639 3386 3654 210
Size: 790 1207 432 747 268 938
Fragments arranged by size:
1207 938 790 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 340 1042 1245 1521 340
Size: 702 203 276 3201
Fragments arranged by size:
3201 702 276 203
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 953 1816 2064 3086 4065 953
Size: 863 248 1022 979 1270
Fragments arranged by size:
1270 1022 979 863 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 183 215 485 517 541 573 1336 1368 2224
Size: 32 270 32 24 32 763 32 856
Cuts at: 2224 2254 2256 2286 183
Size: 30 2 30 2279
Fragments arranged by size:
2279 856 763 270 32 32 32 32
30 30 24 2
HincII GTy'rAC
Cuts at: 918 1421 918
Size: 503 3879
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MfeI C'AATT_G
Cuts at: 626 626
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3237 3053
Size: 184 4198
MscI TGG'CCA
Cuts at: 2143 2143
Size: 4382
MslI CAynn'nnrTG
Cuts at: 210 2371 2498 210
Size: 2161 127 2094
Fragments arranged by size:
2161 2094 127
MspA1I CmG'CkG
Cuts at: 151 174 188 229 235 692 1302 1374 1493
Size: 23 14 41 6 457 610 72 119
Cuts at: 1493 1807 2167 3180 3425 151
Size: 314 360 1013 245 1108
Fragments arranged by size:
1108 1013 610 457 360 314 245 119
72 41 23 14 6
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 205 205
Size: 4382
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NspI r_CATG'y
Cuts at: 265 1435 1935 2842 265
Size: 1170 500 907 1805
Fragments arranged by size:
1805 1170 907 500
PasI CC'CwG_GG
Cuts at: 267 267
Size: 4382
PciI A'CATG_T
Cuts at: 2838 2838
Size: 4382
PflMI CCAn_nnn'nTGG
Cuts at: 188 1374 188
Size: 1186 3196
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 4350
PpuMI rG'GwC_Cy
Cuts at: 1042 1042
Size: 4382
PstI C_TGCA'G
Cuts at: 234 1342 1429 2114 234
Size: 1108 87 685 2502
Fragments arranged by size:
2502 1108 685 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 188 229 692 1302 1374 1807 2167 188
Size: 41 463 610 72 433 360 2403
Fragments arranged by size:
2403 610 463 433 360 72 41
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SacII CC_GC'GG
Cuts at: 152 152
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 1200 2405 2615 1200
Size: 1205 210 2967
Fragments arranged by size:
2967 1205 210
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 985 1162 3899 985
Size: 177 2737 1468
Fragments arranged by size:
2737 1468 177
SfcI C'TryA_G
Cuts at: 33 230 1338 1425 2110 3103 3294 33
Size: 197 1108 87 685 993 191 1121
Fragments arranged by size:
1121 1108 993 685 197 191 87
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 281 856 1166 1537 2452 2944 3206 281
Size: 575 310 371 915 492 262 1457
Fragments arranged by size:
1457 915 575 492 371 310 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 265 1435 1935 265
Size: 1170 500 2712
Fragments arranged by size:
2712 1170 500
SspI AAT'ATT
Cuts at: 320 320
Size: 4382
StyI C'CwwG_G
Cuts at: 205 1016 1178 1516 1870 205
Size: 811 162 338 354 2717
Fragments arranged by size:
2717 811 354 338 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 407 1965 2098 2557 2766 407
Size: 1558 133 459 209 2023
Fragments arranged by size:
2023 1558 459 209 133
TatI w'GTAC_w
Cuts at: 686 983 1160 3897 686
Size: 297 177 2737 1171
Fragments arranged by size:
2737 1171 297 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 207 239 663 695 785 817 3899 3931 207
Size: 32 424 32 90 32 3082 32 658
Fragments arranged by size:
3082 658 424 90 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1371 1877 1371
Size: 506 3876
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BsiWI BsmBI BsrGI BstEII BstZ17I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI