Restriction map for
pF1KB3815
(Circular) (Six-base) MAPSORT of: pF1KB3815.seq Check: 8586 from: 1 to: 4322
pF1KB3815 4322 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1224 1224
Size: 4322
AccI GT'mk_AC
Cuts at: 310 1048 1360 310
Size: 738 312 3272
Fragments arranged by size:
3272 738 312
Acc65I G'GTAC_C
Cuts at: 1338 1338
Size: 4322
AclI AA'CG_TT
Cuts at: 4162 4162
Size: 4322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 2579 3326 3594 2147
Size: 432 747 268 2875
Fragments arranged by size:
2875 747 432 268
AflIII A'CryG_T
Cuts at: 1674 2778 3755 1674
Size: 1104 977 2241
Fragments arranged by size:
2241 1104 977
AgeI A'CCGG_T
Cuts at: 2710 2710
Size: 4322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 266 266
Size: 4322
AlwNI CAG_nnn'CTG
Cuts at: 319 1627 3194 319
Size: 1308 1567 1447
Fragments arranged by size:
1567 1447 1308
ApaLI G'TGCA_C
Cuts at: 3092 3092
Size: 4322
ApoI r'AATT_y
Cuts at: 1303 1326 3675 3931 1303
Size: 23 2349 256 1694
Fragments arranged by size:
2349 1694 256 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4322
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
AvaI C'yCGr_G
Cuts at: 1342 1342
Size: 4322
AvrII C'CTAG_G
Cuts at: 669 669
Size: 4322
BamHI G'GATC_C
Cuts at: 813 1347 813
Size: 534 3788
BanI G'GyrC_C
Cuts at: 469 502 570 801 881 1338 1752 2000 2035
Size: 33 68 231 80 457 414 248 35
Cuts at: 2035 469
Size: 2756
Fragments arranged by size:
2756 457 414 248 231 80 68 35
33
BanII G_rGCy'C
Cuts at: 149 277 1336 2366 149
Size: 128 1059 1030 2105
Fragments arranged by size:
2105 1059 1030 128
BbeI G_GCGC'C
Cuts at: 885 1756 2004 885
Size: 871 248 3203
Fragments arranged by size:
3203 871 248
BbsI GAAGACnn'nnnn_
Cuts at: 315 315
Size: 4322
BbvCI CC'TCA_GC
Cuts at: 740 740
Size: 4322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 186 220 186
Size: 34 4288
BciVI GTATCCnnnnn_n'
Cuts at: 2220 2981 4322 2220
Size: 761 1341 2220
Fragments arranged by size:
2220 1341 761
BclI T'GATC_A
Cuts at: 1842 1842
Size: 4322
BglI GCCn_nnn'nGGC
Cuts at: 1145 1264 1145
Size: 119 4203
BglII A'GATC_T
Cuts at: 602 1837 3874 602
Size: 1235 2037 1050
Fragments arranged by size:
2037 1235 1050
BlpI GC'TnA_GC
Cuts at: 148 1292 1434 148
Size: 1144 142 3036
Fragments arranged by size:
3036 1144 142
Bme1580I G_kGCm'C
Cuts at: 255 507 927 1150 1947 2040 3096 255
Size: 252 420 223 797 93 1056 1481
Fragments arranged by size:
1481 1056 797 420 252 223 93
BmrI ACTGGGnnnn_n'
Cuts at: 1005 1534 1615 1950 4094 1005
Size: 529 81 335 2144 1233
Fragments arranged by size:
2144 1233 529 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 4322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 384 392 640 739 151
Size: 233 8 248 99 3734
Fragments arranged by size:
3734 248 233 99 8
Bpu10I CC'TnA_GC
Cuts at: 740 740
Size: 4322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 750 1171 1498 2377 2869 3167 750
Size: 421 327 879 492 298 1905
Fragments arranged by size:
1905 879 492 421 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 999 4026 999
Size: 3027 1295
BsaAI yAC'GTr
Cuts at: 1675 1675
Size: 4322
BsaBI GATnn'nnATC
Cuts at: 6 262 1836 3873 6
Size: 256 1574 2037 455
Fragments arranged by size:
2037 1574 455 256
BsaHI Gr'CG_yC
Cuts at: 882 1063 1753 2001 2703 4199 882
Size: 181 690 248 702 1496 1005
Fragments arranged by size:
1496 1005 702 690 248 181
BsaWI w'CCGG_w
Cuts at: 990 1511 2032 2710 2984 3131 3542 990
Size: 521 521 678 274 147 411 1770
Fragments arranged by size:
1770 678 521 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 972 1002 972
Size: 30 4292
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 151 148
Size: 3 4319
BseYI C'CCAG_C
Cuts at: 735 793 958 1066 1143 1747 2505 3082 3578
Size: 58 165 108 77 604 758 577 496
Cuts at: 3578 735
Size: 1479
Fragments arranged by size:
1479 758 604 577 496 165 108 77
58
BsiEI CG_ry'CG
Cuts at: 104 1864 1910 3118 104
Size: 1760 46 1208 1308
Fragments arranged by size:
1760 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 149 1336 2114 2304 3096 149
Size: 1187 778 190 792 1375
Fragments arranged by size:
1375 1187 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1030 1030
Size: 4322
Bsp1286I G_dGCh'C
Cuts at: 149 255 277 507 927 1150 1336 1947 2040
Size: 106 22 230 420 223 186 611 93
Cuts at: 2040 2114 2304 2366 3096 149
Size: 74 190 62 730 1375
Fragments arranged by size:
1375 730 611 420 230 223 190 186
106 93 74 62 22
BspEI T'CCGG_A
Cuts at: 3542 3542
Size: 4322
BspHI T'CATG_A
Cuts at: 3498 3498
Size: 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 536 1094 1224 1372 1586 1888 2269 536
Size: 558 130 148 214 302 381 2589
Fragments arranged by size:
2589 558 381 302 214 148 130
BsrBI CCG'CTC
Cuts at: 50 113 966 1296 2614 2668 4152 4317 50
Size: 63 853 330 1318 54 1484 165 55
Fragments arranged by size:
1484 1318 853 330 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 2234 2234
Size: 4322
BsrFI r'CCGG_y
Cuts at: 2320 2501 2710 2320
Size: 181 209 3932
Fragments arranged by size:
3932 209 181
BssSI C'ACGA_G
Cuts at: 2593 2951 2593
Size: 358 3964
BstAPI GCAn_nnn'nTGC
Cuts at: 1699 1699
Size: 4322
BstBI TT'CG_AA
Cuts at: 2683 2683
Size: 4322
BstEII G'GTnAC_C
Cuts at: 744 744
Size: 4322
BstXI CCAn_nnnn'nTGG
Cuts at: 1005 1005
Size: 4322
BstYI r'GATC_y
Cuts at: 1 485 602 813 905 1347 1814 1837 2172
Size: 484 117 211 92 442 467 23 335
Cuts at: 2172 2418 3430 3516 3528 3874 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 484 467 449 442 346 335 246
211 117 92 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 984 2272 2453 2616 984
Size: 1288 181 163 2690
Fragments arranged by size:
2690 1288 181 163
BtsI GCAGTG_nn'
Cuts at: 255 1022 1253 1565 255
Size: 767 231 312 3012
Fragments arranged by size:
3012 767 312 231
DraI TTT'AAA
Cuts at: 1321 1321
Size: 4322
DrdI GACnn_nn'nnGTC
Cuts at: 1044 2028 2886 1044
Size: 984 858 2480
Fragments arranged by size:
2480 984 858
EaeI y'GGCC_r
Cuts at: 1040 1907 2081 2472 2499 3844 1040
Size: 867 174 391 27 1345 1518
Fragments arranged by size:
1518 1345 867 391 174 27
EagI C'GGCC_G
Cuts at: 1907 1907
Size: 4322
EarI CTCTTCn'nnn_
Cuts at: 2345 2555 2345
Size: 210 4112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2530 2840 2986 4132 2530
Size: 310 146 1146 2720
Fragments arranged by size:
2720 1146 310 146
EcoICRI GAG'CTC
Cuts at: 147 1334 147
Size: 1187 3135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 384 392 640 739 2147 2579 3326 3594
Size: 233 8 248 99 1408 432 747 268
Cuts at: 3594 151
Size: 879
Fragments arranged by size:
1408 879 747 432 268 248 233 99
8
EcoO109I rG'GnC_Cy
Cuts at: 363 525 1461 363
Size: 162 936 3224
Fragments arranged by size:
3224 936 162
EcoRI G'AATT_C
Cuts at: 1326 1326
Size: 4322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 4322
FspI TGC'GCA
Cuts at: 2103 2103
Size: 4322
HaeII r_GCGC'y
Cuts at: 885 959 1756 2004 3026 4005 885
Size: 74 797 248 1022 979 1202
Fragments arranged by size:
1202 1022 979 797 248 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 629 661 2164 2194 2196 2226 629
Size: 32 1503 30 2 30 2725
Fragments arranged by size:
2725 1503 32 30 30 2
HincII GTy'rAC
Cuts at: 1361 1361
Size: 4322
KasI G'GCGC_C
Cuts at: 881 1752 2000 881
Size: 871 248 3203
Fragments arranged by size:
3203 871 248
KpnI G_GTAC'C
Cuts at: 1342 1342
Size: 4322
MluI A'CGCG_T
Cuts at: 3755 3755
Size: 4322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1196 2993 3177 1196
Size: 1797 184 2341
Fragments arranged by size:
2341 1797 184
MscI TGG'CCA
Cuts at: 2083 2083
Size: 4322
MslI CAynn'nnrTG
Cuts at: 2311 2438 2311
Size: 127 4195
MspA1I CmG'CkG
Cuts at: 319 739 1433 1747 2107 3120 3365 319
Size: 420 694 314 360 1013 245 1276
Fragments arranged by size:
1276 1013 694 420 360 314 245
NaeI GCC'GGC
Cuts at: 2503 2503
Size: 4322
NarI GG'CG_CC
Cuts at: 882 1753 2001 882
Size: 871 248 3203
Fragments arranged by size:
3203 871 248
NgoMIV G'CCGG_C
Cuts at: 2501 2501
Size: 4322
NspI r_CATG'y
Cuts at: 973 1375 1875 2782 973
Size: 402 500 907 2513
Fragments arranged by size:
2513 907 500 402
PasI CC'CwG_GG
Cuts at: 1225 1225
Size: 4322
PciI A'CATG_T
Cuts at: 2778 2778
Size: 4322
PfoI T'CCnGG_A
Cuts at: 236 617 236
Size: 381 3941
PmeI GTTT'AAAC
Cuts at: 1321 1321
Size: 4322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 3461
Size: 32 4290
PpuMI rG'GwC_Cy
Cuts at: 363 525 363
Size: 162 4160
PstI C_TGCA'G
Cuts at: 1369 2054 1369
Size: 685 3637
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4322
PvuII CAG'CTG
Cuts at: 319 739 1747 2107 319
Size: 420 1008 360 2534
Fragments arranged by size:
2534 1008 420 360
RsrII CG'GwC_CG
Cuts at: 2517 2517
Size: 4322
SacI G_AGCT'C
Cuts at: 149 1336 149
Size: 1187 3135
SalI G'TCGA_C
Cuts at: 1359 1359
Size: 4322
SapI GCTCTTCn'nnn_
Cuts at: 2345 2555 2345
Size: 210 4112
SbfI CC_TGCA'GG
Cuts at: 1369 1369
Size: 4322
ScaI AGT'ACT
Cuts at: 3839 3839
Size: 4322
SfcI C'TryA_G
Cuts at: 33 311 1365 2050 3043 3234 33
Size: 278 1054 685 993 191 1121
Fragments arranged by size:
1121 1054 993 685 278 191
SfoI GGC'GCC
Cuts at: 883 1754 2002 883
Size: 871 248 3203
Fragments arranged by size:
3203 871 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
SmaI CCC'GGG
Cuts at: 1344 1344
Size: 4322
SmlI C'TyrA_G
Cuts at: 765 1186 1477 2392 2884 3146 765
Size: 421 291 915 492 262 1941
Fragments arranged by size:
1941 915 492 421 291 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4322
SphI G_CATG'C
Cuts at: 1375 1875 1375
Size: 500 3822
StuI AGG'CCT
Cuts at: 405 405
Size: 4322
StyI C'CwwG_G
Cuts at: 669 1456 1810 669
Size: 787 354 3181
Fragments arranged by size:
3181 787 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 645 807 1905 2038 2497 2706 645
Size: 162 1098 133 459 209 2261
Fragments arranged by size:
2261 1098 459 209 162 133
TatI w'GTAC_w
Cuts at: 3837 3837
Size: 4322
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 972 1004 3839 3871 972
Size: 32 2835 32 1423
Fragments arranged by size:
2835 1423 32 32
Tth111I GACn'n_nGTC
Cuts at: 2119 2119
Size: 4322
XbaI T'CTAG_A
Cuts at: 1032 1353 1032
Size: 321 4001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 718 795 1200 1817 718
Size: 77 405 617 3223
Fragments arranged by size:
3223 617 405 77
XmaI C'CCGG_G
Cuts at: 1342 1342
Size: 4322
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
BaeI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsrGI
BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI