Restriction map for
pF1KB3799
(Circular) (Six-base) MAPSORT of: pF1KB3799.seq Check: 5380 from: 1 to: 4568
pF1KB3799 4568 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1268 1606 1268
Size: 338 4230
Acc65I G'GTAC_C
Cuts at: 1584 1584
Size: 4568
AclI AA'CG_TT
Cuts at: 172 4408 172
Size: 4236 332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 2393 2825 3572 3840 1312
Size: 1081 432 747 268 2040
Fragments arranged by size:
2040 1081 747 432 268
AfeI AGC'GCT
Cuts at: 254 301 888 254
Size: 47 587 3934
Fragments arranged by size:
3934 587 47
AflIII A'CryG_T
Cuts at: 155 560 1397 1920 3024 4001 155
Size: 405 837 523 1104 977 722
Fragments arranged by size:
1104 977 837 722 523 405
AgeI A'CCGG_T
Cuts at: 2956 2956
Size: 4568
AleI CACnn'nnGTG
Cuts at: 758 937 758
Size: 179 4389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 232 532 232
Size: 300 4268
AlwNI CAG_nnn'CTG
Cuts at: 219 270 291 1873 3440 219
Size: 51 21 1582 1567 1347
Fragments arranged by size:
1582 1567 1347 51 21
ApaI G_GGCC'C
Cuts at: 311 414 423 311
Size: 103 9 4456
Fragments arranged by size:
4456 103 9
ApaLI G'TGCA_C
Cuts at: 1405 1466 3338 1405
Size: 61 1872 2635
Fragments arranged by size:
2635 1872 61
ApoI r'AATT_y
Cuts at: 1572 3921 4177 1572
Size: 2349 256 1963
Fragments arranged by size:
2349 1963 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4568
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
AvaI C'yCGr_G
Cuts at: 407 1208 1588 407
Size: 801 380 3387
Fragments arranged by size:
3387 801 380
BamHI G'GATC_C
Cuts at: 1212 1593 1212
Size: 381 4187
BanI G'GyrC_C
Cuts at: 242 428 516 714 1584 1998 2246 2281 242
Size: 186 88 198 870 414 248 35 2529
Fragments arranged by size:
2529 870 414 248 198 186 88 35
BanII G_rGCy'C
Cuts at: 311 414 423 1006 1165 1321 1582 2612 311
Size: 103 9 583 159 156 261 1030 2267
Fragments arranged by size:
2267 1030 583 261 159 156 103 9
BbeI G_GCGC'C
Cuts at: 2002 2250 2002
Size: 248 4320
BbsI GAAGACnn'nnnn_
Cuts at: 1083 1083
Size: 4568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 333 367 562 596 1521 1555 333
Size: 34 195 34 925 34 3346
Fragments arranged by size:
3346 925 195 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2466 3227 4568 2466
Size: 761 1341 2466
Fragments arranged by size:
2466 1341 761
BclI T'GATC_A
Cuts at: 2088 2088
Size: 4568
BglI GCCn_nnn'nGGC
Cuts at: 418 418
Size: 4568
BglII A'GATC_T
Cuts at: 2083 4120 2083
Size: 2037 2531
BlpI GC'TnA_GC
Cuts at: 1680 1680
Size: 4568
Bme1580I G_kGCm'C
Cuts at: 245 311 414 423 431 521 1409 1470 2193
Size: 66 103 9 8 90 888 61 723
Cuts at: 2193 2286 3342 245
Size: 93 1056 1471
Fragments arranged by size:
1471 1056 888 723 103 93 90 66
61 9 8
BmgBI CAC'GTC
Cuts at: 524 563 524
Size: 39 4529
BmrI ACTGGGnnnn_n'
Cuts at: 1780 1861 2196 4340 1780
Size: 81 335 2144 2008
Fragments arranged by size:
2144 2008 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1335 2935 1335
Size: 1600 2968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 990 1500 1566 990
Size: 510 66 3992
Fragments arranged by size:
3992 510 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1744 2623 3115 3413 1744
Size: 879 492 298 2899
Fragments arranged by size:
2899 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4272 4272
Size: 4568
BsaAI yAC'GTr
Cuts at: 618 1921 618
Size: 1303 3265
BsaBI GATnn'nnATC
Cuts at: 6 2082 4119 6
Size: 2076 2037 455
Fragments arranged by size:
2076 2037 455
BsaHI Gr'CG_yC
Cuts at: 1523 1999 2247 2949 4445 1523
Size: 476 248 702 1496 1646
Fragments arranged by size:
1646 1496 702 476 248
BsaWI w'CCGG_w
Cuts at: 1757 2278 2956 3230 3377 3788 1757
Size: 521 678 274 147 411 2537
Fragments arranged by size:
2537 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 238 249 291 1993 2751 3328 3824 238
Size: 11 42 1702 758 577 496 982
Fragments arranged by size:
1702 982 758 577 496 42 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 888 888
Size: 4568
BsiEI CG_ry'CG
Cuts at: 104 2110 2156 3364 104
Size: 2006 46 1208 1308
Fragments arranged by size:
2006 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 333 1321 1409 1470 1582 2360 2550 3342 333
Size: 988 88 61 112 778 190 792 1559
Fragments arranged by size:
1559 988 792 778 190 112 88 61
BsmI GAATG_Cn'
Cuts at: 234 234
Size: 4568
Bsp1286I G_dGCh'C
Cuts at: 245 311 333 414 423 431 521 1006 1165
Size: 66 22 81 9 8 90 485 159
Cuts at: 1165 1321 1409 1470 1582 2193 2286 2360 2550
Size: 156 88 61 112 611 93 74 190
Cuts at: 2550 2612 3342 245
Size: 62 730 1471
Fragments arranged by size:
1471 730 611 485 190 159 156 112
93 90 88 81 74 66 62 61
22 9 8
BspEI T'CCGG_A
Cuts at: 3788 3788
Size: 4568
BspHI T'CATG_A
Cuts at: 968 3744 968
Size: 2776 1792
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1618 1832 2134 2515 1618
Size: 214 302 381 3671
Fragments arranged by size:
3671 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1425 2860 2914 4398 4563 50
Size: 1375 1435 54 1484 165 55
Fragments arranged by size:
1484 1435 1375 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2480 2480
Size: 4568
BsrFI r'CCGG_y
Cuts at: 203 1055 2566 2747 2956 203
Size: 852 1511 181 209 1815
Fragments arranged by size:
1815 1511 852 209 181
BssSI C'ACGA_G
Cuts at: 332 802 2839 3197 332
Size: 470 2037 358 1703
Fragments arranged by size:
2037 1703 470 358
BstAPI GCAn_nnn'nTGC
Cuts at: 960 1945 960
Size: 985 3583
BstBI TT'CG_AA
Cuts at: 2929 2929
Size: 4568
BstXI CCAn_nnnn'nTGG
Cuts at: 760 816 1143 1533 760
Size: 56 327 390 3795
Fragments arranged by size:
3795 390 327 56
BstYI r'GATC_y
Cuts at: 1 869 1212 1593 2060 2083 2418 2664 3676
Size: 868 343 381 467 23 335 246 1012
Cuts at: 3676 3762 3774 4120 1
Size: 86 12 346 449
Fragments arranged by size:
1012 868 467 449 381 346 343 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 271 271
Size: 4568
BtgI C'CryG_G
Cuts at: 107 113 423 548 759 1080 107
Size: 6 310 125 211 321 3595
Fragments arranged by size:
3595 321 310 211 125 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2518 2699 2862 2518
Size: 181 163 4224
Fragments arranged by size:
4224 181 163
BtsI GCAGTG_nn'
Cuts at: 143 333 1092 1219 1395 1811 143
Size: 190 759 127 176 416 2900
Fragments arranged by size:
2900 759 416 190 176 127
DraI TTT'AAA
Cuts at: 1567 1567
Size: 4568
DraIII CAC_nnn'GTG
Cuts at: 960 960
Size: 4568
DrdI GACnn_nn'nnGTC
Cuts at: 2274 3132 2274
Size: 858 3710
EaeI y'GGCC_r
Cuts at: 110 551 1053 1112 1430 2153 2327 2718 2745
Size: 441 502 59 318 723 174 391 27
Cuts at: 2745 4090 110
Size: 1345 588
Fragments arranged by size:
1345 723 588 502 441 391 318 174
59 27
EagI C'GGCC_G
Cuts at: 2153 2153
Size: 4568
EarI CTCTTCn'nnn_
Cuts at: 1288 2591 2801 1288
Size: 1303 210 3055
Fragments arranged by size:
3055 1303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 190 1351 2776 3086 3232 4378 190
Size: 1161 1425 310 146 1146 380
Fragments arranged by size:
1425 1161 1146 380 310 146
EcoICRI GAG'CTC
Cuts at: 1319 1580 1319
Size: 261 4307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 990 1312 1500 1566 2393 2825 3572 3840 990
Size: 322 188 66 827 432 747 268 1718
Fragments arranged by size:
1718 827 747 432 322 268 188 66
EcoO109I rG'GnC_Cy
Cuts at: 308 411 419 500 1280 1370 1707 308
Size: 103 8 81 780 90 337 3169
Fragments arranged by size:
3169 780 337 103 90 81 8
EcoRI G'AATT_C
Cuts at: 1572 1572
Size: 4568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1317 2587 1317
Size: 1270 3298
FspI TGC'GCA
Cuts at: 832 1333 2349 832
Size: 501 1016 3051
Fragments arranged by size:
3051 1016 501
FspAI rTGC'GCAy
Cuts at: 832 832
Size: 4568
HaeII r_GCGC'y
Cuts at: 256 303 890 2002 2250 3272 4251 256
Size: 47 587 1112 248 1022 979 573
Fragments arranged by size:
1112 1022 979 587 573 248 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1308 1340 2410 2440 2442 2472 1308
Size: 32 1070 30 2 30 3404
Fragments arranged by size:
3404 1070 32 30 30 2
HincII GTy'rAC
Cuts at: 921 1446 1607 921
Size: 525 161 3882
Fragments arranged by size:
3882 525 161
HindIII A'AGCT_T
Cuts at: 1299 1299
Size: 4568
KasI G'GCGC_C
Cuts at: 1998 2246 1998
Size: 248 4320
KpnI G_GTAC'C
Cuts at: 1588 1588
Size: 4568
MluI A'CGCG_T
Cuts at: 155 4001 155
Size: 3846 722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 794 3239 3423 794
Size: 2445 184 1939
Fragments arranged by size:
2445 1939 184
MscI TGG'CCA
Cuts at: 1114 2329 1114
Size: 1215 3353
MslI CAynn'nnrTG
Cuts at: 620 758 802 937 1402 2557 2684 620
Size: 138 44 135 465 1155 127 2504
Fragments arranged by size:
2504 1155 465 138 135 127 44
MspA1I CmG'CkG
Cuts at: 115 216 288 462 711 1290 1529 1679 1993
Size: 101 72 174 249 579 239 150 314
Cuts at: 1993 2353 3366 3611 115
Size: 360 1013 245 1072
Fragments arranged by size:
1072 1013 579 360 314 249 245 239
174 150 101 72
NaeI GCC'GGC
Cuts at: 205 1057 2749 205
Size: 852 1692 2024
Fragments arranged by size:
2024 1692 852
NarI GG'CG_CC
Cuts at: 1999 2247 1999
Size: 248 4320
NcoI C'CATG_G
Cuts at: 107 548 107
Size: 441 4127
NgoMIV G'CCGG_C
Cuts at: 203 1055 2747 203
Size: 852 1692 2024
Fragments arranged by size:
2024 1692 852
NspI r_CATG'y
Cuts at: 597 967 1401 1621 2121 3028 597
Size: 370 434 220 500 907 2137
Fragments arranged by size:
2137 907 500 434 370 220
PasI CC'CwG_GG
Cuts at: 414 414
Size: 4568
PciI A'CATG_T
Cuts at: 1397 3024 1397
Size: 1627 2941
PflMI CCAn_nnn'nTGG
Cuts at: 618 618
Size: 4568
PmeI GTTT'AAAC
Cuts at: 1567 1567
Size: 4568
PmlI CAC'GTG
Cuts at: 618 618
Size: 4568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4536
PpuMI rG'GwC_Cy
Cuts at: 1280 1280
Size: 4568
PspOMI G'GGCC_C
Cuts at: 307 410 419 307
Size: 103 9 4456
Fragments arranged by size:
4456 103 9
PstI C_TGCA'G
Cuts at: 467 1289 1457 1615 2300 467
Size: 822 168 158 685 2735
Fragments arranged by size:
2735 822 685 168 158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4568
PvuII CAG'CTG
Cuts at: 216 288 462 711 1290 1993 2353 216
Size: 72 174 249 579 703 360 2431
Fragments arranged by size:
2431 703 579 360 249 174 72
RsrII CG'GwC_CG
Cuts at: 2763 2763
Size: 4568
SacI G_AGCT'C
Cuts at: 1321 1582 1321
Size: 261 4307
SacII CC_GC'GG
Cuts at: 116 116
Size: 4568
SalI G'TCGA_C
Cuts at: 1605 1605
Size: 4568
SanDI GG'GwC_CC
Cuts at: 1280 1280
Size: 4568
SapI GCTCTTCn'nnn_
Cuts at: 2591 2801 2591
Size: 210 4358
SbfI CC_TGCA'GG
Cuts at: 1615 1615
Size: 4568
ScaI AGT'ACT
Cuts at: 4085 4085
Size: 4568
SfcI C'TryA_G
Cuts at: 33 339 463 1257 1285 1453 1611 2296 3289
Size: 306 124 794 28 168 158 685 993
Cuts at: 3289 3480 33
Size: 191 1121
Fragments arranged by size:
1121 993 794 685 306 191 168 158
124 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 418 418
Size: 4568
SfoI GGC'GCC
Cuts at: 2000 2248 2000
Size: 248 4320
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
SgrAI Cr'CCGG_yG
Cuts at: 203 203
Size: 4568
SmaI CCC'GGG
Cuts at: 1210 1590 1210
Size: 380 4188
SmlI C'TyrA_G
Cuts at: 1723 2638 3130 3392 1723
Size: 915 492 262 2899
Fragments arranged by size:
2899 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4568
SphI G_CATG'C
Cuts at: 1621 2121 1621
Size: 500 4068
StyI C'CwwG_G
Cuts at: 107 233 548 634 1433 1702 2056 107
Size: 126 315 86 799 269 354 2619
Fragments arranged by size:
2619 799 354 315 269 126 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 674 741 2151 2284 2743 2952 674
Size: 67 1410 133 459 209 2290
Fragments arranged by size:
2290 1410 459 209 133 67
TatI w'GTAC_w
Cuts at: 4083 4083
Size: 4568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4085 4117 4085
Size: 32 4536
Tth111I GACn'n_nGTC
Cuts at: 2365 2365
Size: 4568
XbaI T'CTAG_A
Cuts at: 1599 1599
Size: 4568
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1143 2063 1143
Size: 920 3648
XmaI C'CCGG_G
Cuts at: 1208 1588 1208
Size: 380 4188
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsaXI BseRI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV
FseI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PshAI PsiI PspXI PsrI SexAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI