Restriction map for
pF1KB3783
(Circular) (Six-base) MAPSORT of: pF1KB3783.seq Check: 97 from: 1 to: 5387
pF1KB3783 5387 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 1718 2126 1718
Size: 408 4979
AccI GT'mk_AC
Cuts at: 2425 2425
Size: 5387
Acc65I G'GTAC_C
Cuts at: 2403 2403
Size: 5387
AclI AA'CG_TT
Cuts at: 5227 5227
Size: 5387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 757 1801 1951 3212 3644 3997 4659 388
Size: 369 1044 150 1261 432 353 662 1116
Fragments arranged by size:
1261 1116 1044 662 432 369 353 150
AflII C'TTAA_G
Cuts at: 460 460
Size: 5387
AflIII A'CryG_T
Cuts at: 2739 4539 4820 2739
Size: 1800 281 3306
Fragments arranged by size:
3306 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 909 2127 909
Size: 1218 4169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 652 684 652
Size: 32 5355
AlwNI CAG_nnn'CTG
Cuts at: 126 2163 2692 4130 126
Size: 2037 529 1438 1383
Fragments arranged by size:
2037 1438 1383 529
ApaI G_GGCC'C
Cuts at: 206 206
Size: 5387
ApaLI G'TGCA_C
Cuts at: 1722 2245 4225 1722
Size: 523 1980 2884
Fragments arranged by size:
2884 1980 523
ApoI r'AATT_y
Cuts at: 2391 4740 4996 2391
Size: 2349 256 2782
Fragments arranged by size:
2782 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5387
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5387
AvaI C'yCGr_G
Cuts at: 865 1231 1275 2407 865
Size: 366 44 1132 3845
Fragments arranged by size:
3845 1132 366 44
BamHI G'GATC_C
Cuts at: 271 385 1976 2412 271
Size: 114 1591 436 3246
Fragments arranged by size:
3246 1591 436 114
BanI G'GyrC_C
Cuts at: 861 2403 2817 3065 3100 861
Size: 1542 414 248 35 3148
Fragments arranged by size:
3148 1542 414 248 35
BanII G_rGCy'C
Cuts at: 206 1059 1343 1456 1664 1822 2321 2401 3431
Size: 853 284 113 208 158 499 80 1030
Cuts at: 3431 206
Size: 2162
Fragments arranged by size:
2162 1030 853 499 284 208 158 113
80
BbeI G_GCGC'C
Cuts at: 865 2821 3069 865
Size: 1956 248 3183
Fragments arranged by size:
3183 1956 248
BbsI GAAGACnn'nnnn_
Cuts at: 221 221
Size: 5387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2160 2194 90
Size: 34 2036 34 3283
Fragments arranged by size:
3283 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3285 4341 5387 3285
Size: 1056 1046 3285
Fragments arranged by size:
3285 1056 1046
BclI T'GATC_A
Cuts at: 2907 2907
Size: 5387
BglII A'GATC_T
Cuts at: 732 1353 2902 4939 732
Size: 621 1549 2037 1180
Fragments arranged by size:
2037 1549 1180 621
BlpI GC'TnA_GC
Cuts at: 680 2230 2342 2499 680
Size: 1550 112 157 3568
Fragments arranged by size:
3568 1550 157 112
Bme1580I G_kGCm'C
Cuts at: 206 773 1726 2249 3012 3105 4229 206
Size: 567 953 523 763 93 1124 1364
Fragments arranged by size:
1364 1124 953 763 567 523 93
BmgBI CAC'GTC
Cuts at: 2244 2369 2244
Size: 125 5262
BmrI ACTGGGnnnn_n'
Cuts at: 348 1363 2103 2599 2680 3015 5159 348
Size: 1015 740 496 81 335 2144 576
Fragments arranged by size:
2144 1015 740 576 496 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 634 3754 634
Size: 3120 2267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1986 2335 1986
Size: 349 5038
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1308 1582 2563 3442 4156 4454 1308
Size: 274 981 879 714 298 2241
Fragments arranged by size:
2241 981 879 714 298 274
BsaI GGTCTCn'nnnn_
Cuts at: 163 973 1529 1769 5091 163
Size: 810 556 240 3322 459
Fragments arranged by size:
3322 810 556 459 240
BsaAI yAC'GTr
Cuts at: 2145 2740 2145
Size: 595 4792
BsaBI GATnn'nnATC
Cuts at: 6 307 1555 2901 4938 6
Size: 301 1248 1346 2037 455
Fragments arranged by size:
2037 1346 1248 455 301
BsaHI Gr'CG_yC
Cuts at: 290 862 1249 1268 1715 2123 2818 3066 3768
Size: 572 387 19 447 408 695 248 702
Cuts at: 3768 5264 290
Size: 1496 413
Fragments arranged by size:
1496 702 695 572 447 413 408 387
248 19
BsaWI w'CCGG_w
Cuts at: 1163 2576 3097 3775 4186 4333 4607 1163
Size: 1413 521 678 411 147 274 1943
Fragments arranged by size:
1943 1413 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1245 1275 1245
Size: 30 5357
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 670 2056 193
Size: 477 1386 3524
Fragments arranged by size:
3524 1386 477
BseYI C'CCAG_C
Cuts at: 433 1008 1086 2812 3570 4235 4643 433
Size: 575 78 1726 758 665 408 1177
Fragments arranged by size:
1726 1177 758 665 575 408 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 1405 1342
Size: 63 5324
BsiEI CG_ry'CG
Cuts at: 104 851 2929 2975 4205 104
Size: 747 2078 46 1230 1286
Fragments arranged by size:
2078 1286 1230 747 46
BsiHKAI G_wGCw'C
Cuts at: 926 1059 1664 1726 2183 2249 2401 3179 3369
Size: 133 605 62 457 66 152 778 190
Cuts at: 3369 4229 926
Size: 860 2084
Fragments arranged by size:
2084 860 778 605 457 190 152 133
66 62
Bsp1286I G_dGCh'C
Cuts at: 206 773 926 1059 1343 1456 1664 1726 1822
Size: 567 153 133 284 113 208 62 96
Cuts at: 1822 2183 2249 2321 2401 3012 3105 3179 3369
Size: 361 66 72 80 611 93 74 190
Cuts at: 3369 3431 4229 206
Size: 62 798 1364
Fragments arranged by size:
1364 798 611 567 361 284 208 190
153 133 113 96 93 80 74 72
66 62 62
BspHI T'CATG_A
Cuts at: 1886 3819 1886
Size: 1933 3454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2437 2651 2953 3334 2437
Size: 214 302 381 4490
Fragments arranged by size:
4490 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3679 3733 5217 5382 50
Size: 3629 54 1484 165 55
Fragments arranged by size:
3629 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3299 3299
Size: 5387
BsrFI r'CCGG_y
Cuts at: 878 1082 3385 3566 878
Size: 204 2303 181 2699
Fragments arranged by size:
2699 2303 204 181
BsrGI T'GTAC_A
Cuts at: 1799 1985 1799
Size: 186 5201
BssHII G'CGCG_C
Cuts at: 1866 1866
Size: 5387
BssSI C'ACGA_G
Cuts at: 3658 4366 3658
Size: 708 4679
BstAPI GCAn_nnn'nTGC
Cuts at: 1356 2764 1356
Size: 1408 3979
BstBI TT'CG_AA
Cuts at: 3748 3748
Size: 5387
BstXI CCAn_nnnn'nTGG
Cuts at: 1740 1914 2379 1740
Size: 174 465 4748
Fragments arranged by size:
4748 465 174
BstYI r'GATC_y
Cuts at: 1 183 271 345 385 732 1353 1556 1976
Size: 182 88 74 40 347 621 203 420
Cuts at: 1976 2412 2879 2902 3237 3483 3789 3801 3887
Size: 436 467 23 335 246 306 12 86
Cuts at: 3887 4939 1
Size: 1052 449
Fragments arranged by size:
1052 621 467 449 436 420 347 335
306 246 203 182 88 86 74 40
23 12
Bsu36I CC'TnA_GG
Cuts at: 1208 2039 2300 1208
Size: 831 261 4295
Fragments arranged by size:
4295 831 261
BtgI C'CryG_G
Cuts at: 983 1490 1739 2191 2308 983
Size: 507 249 452 117 4062
Fragments arranged by size:
4062 507 452 249 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1488 2185 2278 3337 3518 3681 1488
Size: 697 93 1059 181 163 3194
Fragments arranged by size:
3194 1059 697 181 163 93
BtsI GCAGTG_nn'
Cuts at: 218 1202 2070 2630 218
Size: 984 868 560 2975
Fragments arranged by size:
2975 984 868 560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2262 2297 2262
Size: 35 5352
DraI TTT'AAA
Cuts at: 2386 2386
Size: 5387
DraIII CAC_nnn'GTG
Cuts at: 2253 2253
Size: 5387
DrdI GACnn_nn'nnGTC
Cuts at: 3093 4437 3093
Size: 1344 4043
EaeI y'GGCC_r
Cuts at: 1572 1904 2267 2972 3146 3537 3564 4909 1572
Size: 332 363 705 174 391 27 1345 2050
Fragments arranged by size:
2050 1345 705 391 363 332 174 27
EagI C'GGCC_G
Cuts at: 2972 2972
Size: 5387
EarI CTCTTCn'nnn_
Cuts at: 625 758 1066 1541 2353 3410 3620 625
Size: 133 308 475 812 1057 210 2392
Fragments arranged by size:
2392 1057 812 475 308 210 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3595 4337 4483 5197 3595
Size: 742 146 714 3785
Fragments arranged by size:
3785 742 714 146
EcoICRI GAG'CTC
Cuts at: 1057 1662 2399 1057
Size: 605 737 4045
Fragments arranged by size:
4045 737 605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 757 1801 1951 1986 2335 3212 3644 3997
Size: 369 1044 150 35 349 877 432 353
Cuts at: 3997 4659 388
Size: 662 1116
Fragments arranged by size:
1116 1044 877 662 432 369 353 349
150 35
EcoNI CCTnn'n_nnAGG
Cuts at: 799 1333 2254 799
Size: 534 921 3932
Fragments arranged by size:
3932 921 534
EcoO109I rG'GnC_Cy
Cuts at: 202 456 615 947 1926 2304 2526 202
Size: 254 159 332 979 378 222 3063
Fragments arranged by size:
3063 979 378 332 254 222 159
EcoRI G'AATT_C
Cuts at: 2391 2391
Size: 5387
EcoRV GAT'ATC
Cuts at: 2105 2105
Size: 5387
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 348 3406 348
Size: 3058 2329
FspI TGC'GCA
Cuts at: 3168 3168
Size: 5387
HaeII r_GCGC'y
Cuts at: 865 1125 2163 2821 3069 4299 5070 865
Size: 260 1038 658 248 1230 771 1182
Fragments arranged by size:
1230 1182 1038 771 658 260 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 895 927 972 1004 2089 2113 2121 2145 2200
Size: 32 45 32 1085 24 8 24 55
Cuts at: 2200 2232 3229 3259 3261 3291 895
Size: 32 997 30 2 30 2991
Fragments arranged by size:
2991 1085 997 55 45 32 32 32
30 30 24 24 8 2
HincII GTy'rAC
Cuts at: 2202 2426 2202
Size: 224 5163
HindIII A'AGCT_T
Cuts at: 550 550
Size: 5387
KasI G'GCGC_C
Cuts at: 861 2817 3065 861
Size: 1956 248 3183
Fragments arranged by size:
3183 1956 248
KpnI G_GTAC'C
Cuts at: 2407 2407
Size: 5387
MluI A'CGCG_T
Cuts at: 4820 4820
Size: 5387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 452 2357 4146 4330 452
Size: 1905 1789 184 1509
Fragments arranged by size:
1905 1789 1509 184
MscI TGG'CCA
Cuts at: 3148 3148
Size: 5387
MslI CAynn'nnrTG
Cuts at: 1447 3376 3503 1447
Size: 1929 127 3331
Fragments arranged by size:
3331 1929 127
MspA1I CmG'CkG
Cuts at: 247 1863 2310 2498 2812 3172 3956 4201 247
Size: 1616 447 188 314 360 784 245 1433
Fragments arranged by size:
1616 1433 784 447 360 314 245 188
NaeI GCC'GGC
Cuts at: 3568 3568
Size: 5387
NarI GG'CG_CC
Cuts at: 862 2818 3066 862
Size: 1956 248 3183
Fragments arranged by size:
3183 1956 248
NcoI C'CATG_G
Cuts at: 983 1739 983
Size: 756 4631
NgoMIV G'CCGG_C
Cuts at: 3566 3566
Size: 5387
NspI r_CATG'y
Cuts at: 2440 2940 4543 2440
Size: 500 1603 3284
Fragments arranged by size:
3284 1603 500
PasI CC'CwG_GG
Cuts at: 1758 1758
Size: 5387
PciI A'CATG_T
Cuts at: 4539 4539
Size: 5387
PflMI CCAn_nnn'nTGG
Cuts at: 714 1148 714
Size: 434 4953
PfoI T'CCnGG_A
Cuts at: 593 755 1334 1664 593
Size: 162 579 330 4316
Fragments arranged by size:
4316 579 330 162
PmeI GTTT'AAAC
Cuts at: 2386 2386
Size: 5387
PmlI CAC'GTG
Cuts at: 2145 2145
Size: 5387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 631 663 3833 3865 631
Size: 32 3170 32 2153
Fragments arranged by size:
3170 2153 32 32
PpuMI rG'GwC_Cy
Cuts at: 456 615 947 1926 456
Size: 159 332 979 3917
Fragments arranged by size:
3917 979 332 159
PshAI GACnn'nnGTC
Cuts at: 2367 2367
Size: 5387
PspOMI G'GGCC_C
Cuts at: 202 202
Size: 5387
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1144 1176 1144
Size: 32 5355
PstI C_TGCA'G
Cuts at: 225 2434 3119 225
Size: 2209 685 2493
Fragments arranged by size:
2493 2209 685
PvuI CG_AT'CG
Cuts at: 104 851 104
Size: 747 4640
PvuII CAG'CTG
Cuts at: 247 1863 2812 3172 247
Size: 1616 949 360 2462
Fragments arranged by size:
2462 1616 949 360
RsrII CG'GwC_CG
Cuts at: 3582 3582
Size: 5387
SacI G_AGCT'C
Cuts at: 1059 1664 2401 1059
Size: 605 737 4045
Fragments arranged by size:
4045 737 605
SacII CC_GC'GG
Cuts at: 2311 2311
Size: 5387
SalI G'TCGA_C
Cuts at: 2424 2424
Size: 5387
SanDI GG'GwC_CC
Cuts at: 947 947
Size: 5387
SapI GCTCTTCn'nnn_
Cuts at: 3410 3620 3410
Size: 210 5177
SbfI CC_TGCA'GG
Cuts at: 2434 2434
Size: 5387
ScaI AGT'ACT
Cuts at: 4904 4904
Size: 5387
SexAI A'CCwGG_T
Cuts at: 1631 2249 1631
Size: 618 4769
SfcI C'TryA_G
Cuts at: 33 221 726 2430 3115 4083 4274 33
Size: 188 505 1704 685 968 191 1146
Fragments arranged by size:
1704 1146 968 685 505 191 188
SfoI GGC'GCC
Cuts at: 863 2819 3067 863
Size: 1956 248 3183
Fragments arranged by size:
3183 1956 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5387
SmaI CCC'GGG
Cuts at: 2409 2409
Size: 5387
SmlI C'TyrA_G
Cuts at: 460 1323 1597 2542 3457 4171 4433 460
Size: 863 274 945 915 714 262 1414
Fragments arranged by size:
1414 945 915 863 714 274 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5387
SphI G_CATG'C
Cuts at: 2440 2940 2440
Size: 500 4887
StuI AGG'CCT
Cuts at: 1206 1206
Size: 5387
StyI C'CwwG_G
Cuts at: 689 983 1739 2521 2875 689
Size: 294 756 782 354 3201
Fragments arranged by size:
3201 782 756 354 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 503 1078 1391 2184 2970 3103 3562 3771 503
Size: 575 313 793 786 133 459 209 2119
Fragments arranged by size:
2119 793 786 575 459 313 209 133
TatI w'GTAC_w
Cuts at: 854 1799 1985 4902 854
Size: 945 186 2917 1339
Fragments arranged by size:
2917 1339 945 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1245 1277 2049 2081 4904 4936 1245
Size: 32 772 32 2823 32 1696
Fragments arranged by size:
2823 1696 772 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3184 3184
Size: 5387
XbaI T'CTAG_A
Cuts at: 2418 2418
Size: 5387
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1417 2882 1417
Size: 1465 3922
XmaI C'CCGG_G
Cuts at: 2407 2407
Size: 5387
ZraI GAC'GTC
Cuts at: 1716 2124 1716
Size: 408 4979
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AleI AlfI AscI AvrII BaeI
BbvCI BfrBI BglI BmtI Bpu10I BsiWI BsmI BsmBI
BspEI BstEII BstZ17I ClaI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PsiI PspXI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI