Restriction map for
pF1KB3774
(Circular) (Six-base) MAPSORT of: pF1KB3774.seq Check: 2627 from: 1 to: 4163
pF1KB3774 4163 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 431 431
Size: 4163
AccI GT'mk_AC
Cuts at: 1145 1201 1145
Size: 56 4107
Acc65I G'GTAC_C
Cuts at: 932 1179 932
Size: 247 3916
AclI AA'CG_TT
Cuts at: 1157 4003 1157
Size: 2846 1317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1988 2420 3167 3435 1988
Size: 432 747 268 2716
Fragments arranged by size:
2716 747 432 268
AflIII A'CryG_T
Cuts at: 992 1515 2619 3596 992
Size: 523 1104 977 1559
Fragments arranged by size:
1559 1104 977 523
AgeI A'CCGG_T
Cuts at: 1049 2551 1049
Size: 1502 2661
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 484 516 484
Size: 32 4131
AlwNI CAG_nnn'CTG
Cuts at: 1468 3035 1468
Size: 1567 2596
ApaI G_GGCC'C
Cuts at: 231 231
Size: 4163
ApaLI G'TGCA_C
Cuts at: 1081 2933 1081
Size: 1852 2311
ApoI r'AATT_y
Cuts at: 1167 3516 3772 1167
Size: 2349 256 1558
Fragments arranged by size:
2349 1558 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 303 401 579 1183 303
Size: 98 178 604 3283
Fragments arranged by size:
3283 604 178 98
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 258 346 651 756 932 1028 1179 1593 1841
Size: 88 305 105 176 96 151 414 248
Cuts at: 1841 1876 258
Size: 35 2545
Fragments arranged by size:
2545 414 305 248 176 151 105 96
88 35
BanII G_rGCy'C
Cuts at: 231 598 1177 2207 231
Size: 367 579 1030 2187
Fragments arranged by size:
2187 1030 579 367
BbeI G_GCGC'C
Cuts at: 655 1597 1845 655
Size: 942 248 2973
Fragments arranged by size:
2973 942 248
BbsI GAAGACnn'nnnn_
Cuts at: 1078 1078
Size: 4163
BbvCI CC'TCA_GC
Cuts at: 286 286
Size: 4163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 328 362 328
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglII A'GATC_T
Cuts at: 872 1678 3715 872
Size: 806 2037 1320
Fragments arranged by size:
2037 1320 806
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 231 263 626 761 1085 1788 1881 2937 231
Size: 32 363 135 324 703 93 1056 1457
Fragments arranged by size:
1457 1056 703 363 324 135 93 32
BmgBI CAC'GTC
Cuts at: 615 615
Size: 4163
BmrI ACTGGGnnnn_n'
Cuts at: 453 826 1047 1375 1456 1791 3935 453
Size: 373 221 328 81 335 2144 681
Fragments arranged by size:
2144 681 373 335 328 221 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 318 2530 318
Size: 2212 1951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 4163
Bpu10I CC'TnA_GC
Cuts at: 286 286
Size: 4163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 2218 2710 3008 1339
Size: 879 492 298 2494
Fragments arranged by size:
2494 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 428 652 1594 1842 2544 4040 428
Size: 224 942 248 702 1496 551
Fragments arranged by size:
1496 942 702 551 248 224
BsaWI w'CCGG_w
Cuts at: 423 1049 1067 1352 1873 2551 2825 2972 3383
Size: 626 18 285 521 678 274 147 411
Cuts at: 3383 423
Size: 1203
Fragments arranged by size:
1203 678 626 521 411 285 274 147
18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 484 514 484
Size: 30 4133
BseYI C'CCAG_C
Cuts at: 882 1588 2346 2923 3419 882
Size: 706 758 577 496 1626
Fragments arranged by size:
1626 758 706 577 496
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 118 1085 1177 1955 2145 2937 118
Size: 967 92 778 190 792 1344
Fragments arranged by size:
1344 967 792 778 190 92
Bsp1286I G_dGCh'C
Cuts at: 118 231 263 598 626 761 1085 1177 1788
Size: 113 32 335 28 135 324 92 611
Cuts at: 1788 1881 1955 2145 2207 2937 118
Size: 93 74 190 62 730 1344
Fragments arranged by size:
1344 730 611 335 324 190 135 113
93 92 74 62 32 28
BspEI T'CCGG_A
Cuts at: 1067 3383 1067
Size: 2316 1847
BspHI T'CATG_A
Cuts at: 635 3339 635
Size: 2704 1459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 596 737 1213 1427 1729 2110 596
Size: 141 476 214 302 381 2649
Fragments arranged by size:
2649 476 381 302 214 141
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 271 343 554 1049 2161 2342 2551 271
Size: 72 211 495 1112 181 209 1883
Fragments arranged by size:
1883 1112 495 211 209 181 72
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstYI r'GATC_y
Cuts at: 1 414 812 872 1188 1655 1678 2013 2259
Size: 413 398 60 316 467 23 335 246
Cuts at: 2259 3271 3357 3369 3715 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 413 398 346 335 316
246 86 60 23 12
BtgI C'CryG_G
Cuts at: 140 566 659 803 941 140
Size: 426 93 144 138 3362
Fragments arranged by size:
3362 426 144 138 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 353 2113 2294 2457 353
Size: 1760 181 163 2059
Fragments arranged by size:
2059 1760 181 163
BtsI GCAGTG_nn'
Cuts at: 1406 1406
Size: 4163
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DraIII CAC_nnn'GTG
Cuts at: 249 249
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 200 1121 1748 1922 2313 2340 3685 200
Size: 921 627 174 391 27 1345 678
Fragments arranged by size:
1345 921 678 627 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 181 2186 2396 181
Size: 2005 210 1948
Fragments arranged by size:
2005 1948 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1988 2420 3167 3435 511
Size: 1477 432 747 268 1239
Fragments arranged by size:
1477 1239 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 289 289
Size: 4163
EcoO109I rG'GnC_Cy
Cuts at: 227 228 540 768 1302 227
Size: 1 312 228 534 3088
Fragments arranged by size:
3088 534 312 228 1
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 285 561 655 797 1597 1845 2867 3846 285
Size: 276 94 142 800 248 1022 979 602
Fragments arranged by size:
1022 979 800 602 276 248 142 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 630 662 906 938 2005 2035 2037 2067 630
Size: 32 244 32 1067 30 2 30 2726
Fragments arranged by size:
2726 1067 244 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 651 1593 1841 651
Size: 942 248 2973
Fragments arranged by size:
2973 942 248
KpnI G_GTAC'C
Cuts at: 936 1183 936
Size: 247 3916
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 377 2834 3018 377
Size: 2457 184 1522
Fragments arranged by size:
2457 1522 184
MscI TGG'CCA
Cuts at: 202 1123 1924 202
Size: 921 801 2441
Fragments arranged by size:
2441 921 801
MslI CAynn'nnrTG
Cuts at: 640 946 977 1114 2152 2279 640
Size: 306 31 137 1038 127 2524
Fragments arranged by size:
2524 1038 306 137 127 31
MspA1I CmG'CkG
Cuts at: 329 1274 1588 1948 2961 3206 329
Size: 945 314 360 1013 245 1286
Fragments arranged by size:
1286 1013 945 360 314 245
NaeI GCC'GGC
Cuts at: 273 556 2344 273
Size: 283 1788 2092
Fragments arranged by size:
2092 1788 283
NarI GG'CG_CC
Cuts at: 652 1594 1842 652
Size: 942 248 2973
Fragments arranged by size:
2973 942 248
NgoMIV G'CCGG_C
Cuts at: 271 554 2342 271
Size: 283 1788 2092
Fragments arranged by size:
2092 1788 283
NspI r_CATG'y
Cuts at: 976 1119 1216 1716 2623 976
Size: 143 97 500 907 2516
Fragments arranged by size:
2516 907 500 143 97
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PflMI CCAn_nnn'nTGG
Cuts at: 198 198
Size: 4163
PfoI T'CCnGG_A
Cuts at: 536 770 536
Size: 234 3929
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 484 516 3302 3334 484
Size: 32 2786 32 1313
Fragments arranged by size:
2786 1313 32 32
PpuMI rG'GwC_Cy
Cuts at: 540 768 540
Size: 228 3935
PspOMI G'GGCC_C
Cuts at: 227 227
Size: 4163
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1036 1068 1036
Size: 32 4131
PstI C_TGCA'G
Cuts at: 281 455 1210 1895 281
Size: 174 755 685 2549
Fragments arranged by size:
2549 755 685 174
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 1588 1948 1588
Size: 360 3803
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SanDI GG'GwC_CC
Cuts at: 540 540
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 181 2186 2396 181
Size: 2005 210 1948
Fragments arranged by size:
2005 1948 210
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 277 451 1206 1891 2884 3075 33
Size: 244 174 755 685 993 191 1121
Fragments arranged by size:
1121 993 755 685 244 191 174
SfoI GGC'GCC
Cuts at: 653 1595 1843 653
Size: 942 248 2973
Fragments arranged by size:
2973 942 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 1318 2233 2725 2987 1318
Size: 915 492 262 2494
Fragments arranged by size:
2494 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StyI C'CwwG_G
Cuts at: 1124 1297 1651 1124
Size: 173 354 3636
Fragments arranged by size:
3636 354 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 709 1746 1879 2338 2547 709
Size: 1037 133 459 209 2325
Fragments arranged by size:
2325 1037 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 413 445 766 798 3680 3712 413
Size: 32 321 32 2882 32 864
Fragments arranged by size:
2882 864 321 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 486 511 948 1658 486
Size: 25 437 710 2991
Fragments arranged by size:
2991 710 437 25
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
XmnI GAAnn'nnTTC
Cuts at: 499 841 499
Size: 342 3821
ZraI GAC'GTC
Cuts at: 429 429
Size: 4163
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BfrBI BglI BmtI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI