Restriction map for
pF1KB3769
(Circular) (Six-base) MAPSORT of: pF1KB3769.seq Check: 6885 from: 1 to: 5030
pF1KB3769 5030 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1442 2068 1442
Size: 626 4404
Acc65I G'GTAC_C
Cuts at: 2046 2046
Size: 5030
AclI AA'CG_TT
Cuts at: 1577 4870 1577
Size: 3293 1737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3287 3640 4302 2855
Size: 432 353 662 3583
Fragments arranged by size:
3583 662 432 353
AflIII A'CryG_T
Cuts at: 734 2382 4182 4463 734
Size: 1648 1800 281 1301
Fragments arranged by size:
1800 1648 1301 281
AgeI A'CCGG_T
Cuts at: 1600 1600
Size: 5030
AlwNI CAG_nnn'CTG
Cuts at: 1161 1287 2335 3773 1161
Size: 126 1048 1438 2418
Fragments arranged by size:
2418 1438 1048 126
ApaI G_GGCC'C
Cuts at: 881 881
Size: 5030
ApaLI G'TGCA_C
Cuts at: 526 3868 526
Size: 3342 1688
ApoI r'AATT_y
Cuts at: 340 2034 4383 4639 340
Size: 1694 2349 256 731
Fragments arranged by size:
2349 1694 731 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5030
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
AvaI C'yCGr_G
Cuts at: 2050 2050
Size: 5030
BamHI G'GATC_C
Cuts at: 2055 2055
Size: 5030
BanI G'GyrC_C
Cuts at: 145 595 1231 2046 2460 2708 2743 145
Size: 450 636 815 414 248 35 2432
Fragments arranged by size:
2432 815 636 450 414 248 35
BanII G_rGCy'C
Cuts at: 611 638 881 943 1031 1913 2044 3074 611
Size: 27 243 62 88 882 131 1030 2567
Fragments arranged by size:
2567 1030 882 243 131 88 62 27
BbeI G_GCGC'C
Cuts at: 1235 2464 2712 1235
Size: 1229 248 3553
Fragments arranged by size:
3553 1229 248
BbsI GAAGACnn'nnnn_
Cuts at: 162 1784 162
Size: 1622 3408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 948 982 948
Size: 34 4996
BciVI GTATCCnnnnn_n'
Cuts at: 1153 2928 3984 5030 1153
Size: 1775 1056 1046 1153
Fragments arranged by size:
1775 1153 1056 1046
BclI T'GATC_A
Cuts at: 1847 2550 1847
Size: 703 4327
BglI GCCn_nnn'nGGC
Cuts at: 263 263
Size: 5030
BglII A'GATC_T
Cuts at: 2545 4582 2545
Size: 2037 2993
BlpI GC'TnA_GC
Cuts at: 2142 2142
Size: 5030
Bme1580I G_kGCm'C
Cuts at: 148 530 881 2655 2748 3872 148
Size: 382 351 1774 93 1124 1306
Fragments arranged by size:
1774 1306 1124 382 351 93
BmgBI CAC'GTC
Cuts at: 735 1302 735
Size: 567 4463
BmrI ACTGGGnnnn_n'
Cuts at: 2242 2323 2658 4802 2242
Size: 81 335 2144 2470
Fragments arranged by size:
2470 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 486 987 3397 486
Size: 501 2410 2119
Fragments arranged by size:
2410 2119 501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1762 1078
Size: 684 4346
Bpu10I CC'TnA_GC
Cuts at: 937 937
Size: 5030
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2206 3085 3799 4097 2206
Size: 879 714 298 3139
Fragments arranged by size:
3139 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 433 4734 433
Size: 4301 729
BsaAI yAC'GTr
Cuts at: 234 2383 234
Size: 2149 2881
BsaBI GATnn'nnATC
Cuts at: 6 2544 4581 6
Size: 2538 2037 455
Fragments arranged by size:
2538 2037 455
BsaHI Gr'CG_yC
Cuts at: 269 317 1085 1232 1559 2461 2709 3411 4907
Size: 48 768 147 327 902 248 702 1496
Cuts at: 4907 269
Size: 392
Fragments arranged by size:
1496 902 768 702 392 327 248 147
48
BsaWI w'CCGG_w
Cuts at: 1600 1664 2219 2740 3418 3829 3976 4250 1600
Size: 64 555 521 678 411 147 274 2380
Fragments arranged by size:
2380 678 555 521 411 274 147 64
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 475 859 1039 475
Size: 384 180 4466
Fragments arranged by size:
4466 384 180
BseYI C'CCAG_C
Cuts at: 226 1317 1482 2455 3213 3878 4286 226
Size: 1091 165 973 758 665 408 970
Fragments arranged by size:
1091 973 970 758 665 408 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 1261 1300 1456 1693 379
Size: 882 39 156 237 3716
Fragments arranged by size:
3716 882 237 156 39
BsiEI CG_ry'CG
Cuts at: 104 785 1719 1863 2572 2618 3848 104
Size: 681 934 144 709 46 1230 1286
Fragments arranged by size:
1286 1230 934 709 681 144 46
BsiHKAI G_wGCw'C
Cuts at: 530 704 943 1031 1600 1913 2044 2822 3012
Size: 174 239 88 569 313 131 778 190
Cuts at: 3012 3872 530
Size: 860 1688
Fragments arranged by size:
1688 860 778 569 313 239 190 174
131 88
Bsp1286I G_dGCh'C
Cuts at: 148 530 611 638 704 881 943 1031 1600
Size: 382 81 27 66 177 62 88 569
Cuts at: 1600 1913 2044 2655 2748 2822 3012 3074 3872
Size: 313 131 611 93 74 190 62 798
Cuts at: 3872 148
Size: 1306
Fragments arranged by size:
1306 798 611 569 382 313 190 177
131 93 88 81 74 66 62 62
27
BspHI T'CATG_A
Cuts at: 1343 3462 1343
Size: 2119 2911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1295 1428 1487 2080 2294 2596 2977 1295
Size: 133 59 593 214 302 381 3348
Fragments arranged by size:
3348 593 381 302 214 133 59
BsrBI CCG'CTC
Cuts at: 50 1686 3322 3376 4860 5025 50
Size: 1636 1636 54 1484 165 55
Fragments arranged by size:
1636 1636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2942 2942
Size: 5030
BsrFI r'CCGG_y
Cuts at: 784 1062 1600 3028 3209 784
Size: 278 538 1428 181 2605
Fragments arranged by size:
2605 1428 538 278 181
BssHII G'CGCG_C
Cuts at: 888 921 1011 888
Size: 33 90 4907
Fragments arranged by size:
4907 90 33
BssSI C'ACGA_G
Cuts at: 3301 4009 3301
Size: 708 4322
BstAPI GCAn_nnn'nTGC
Cuts at: 914 1287 2407 914
Size: 373 1120 3537
Fragments arranged by size:
3537 1120 373
BstBI TT'CG_AA
Cuts at: 3391 3391
Size: 5030
BstEII G'GTnAC_C
Cuts at: 1542 1542
Size: 5030
BstXI CCAn_nnnn'nTGG
Cuts at: 234 234
Size: 5030
BstYI r'GATC_y
Cuts at: 1 1106 1151 1823 2055 2522 2545 2880 3126
Size: 1105 45 672 232 467 23 335 246
Cuts at: 3126 3432 3444 3530 4582 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1105 1052 672 467 449 335 306 246
232 86 45 23 12
BstZ17I GTA'TAC
Cuts at: 1443 1443
Size: 5030
BtgI C'CryG_G
Cuts at: 117 473 638 779 1235 117
Size: 356 165 141 456 3912
Fragments arranged by size:
3912 456 356 165 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 180 636 2980 3161 3324 180
Size: 456 2344 181 163 1886
Fragments arranged by size:
2344 1886 456 181 163
BtsI GCAGTG_nn'
Cuts at: 2273 2273
Size: 5030
DraI TTT'AAA
Cuts at: 2029 2029
Size: 5030
DrdI GACnn_nn'nnGTC
Cuts at: 1311 2736 4080 1311
Size: 1425 1344 2261
Fragments arranged by size:
2261 1425 1344
EaeI y'GGCC_r
Cuts at: 287 379 647 782 1247 1727 2615 2789 3180
Size: 92 268 135 465 480 888 174 391
Cuts at: 3180 3207 4552 287
Size: 27 1345 765
Fragments arranged by size:
1345 888 765 480 465 391 268 174
135 92 27
EagI C'GGCC_G
Cuts at: 782 2615 782
Size: 1833 3197
EarI CTCTTCn'nnn_
Cuts at: 3053 3263 3053
Size: 210 4820
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 856 1696 3238 3980 4126 4840 856
Size: 840 1542 742 146 714 1046
Fragments arranged by size:
1542 1046 840 742 714 146
EcoICRI GAG'CTC
Cuts at: 941 1029 1911 2042 941
Size: 88 882 131 3929
Fragments arranged by size:
3929 882 131 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1762 2855 3287 3640 4302 1078
Size: 684 1093 432 353 662 1806
Fragments arranged by size:
1806 1093 684 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 841 841
Size: 5030
EcoO109I rG'GnC_Cy
Cuts at: 1052 1284 1527 2002 2169 1052
Size: 232 243 475 167 3913
Fragments arranged by size:
3913 475 243 232 167
EcoRI G'AATT_C
Cuts at: 2034 2034
Size: 5030
EcoRV GAT'ATC
Cuts at: 467 467
Size: 5030
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 468 3049 468
Size: 2581 2449
FspI TGC'GCA
Cuts at: 622 907 2811 622
Size: 285 1904 2841
Fragments arranged by size:
2841 1904 285
HaeII r_GCGC'y
Cuts at: 258 576 1078 1235 2464 2712 3942 4713 258
Size: 318 502 157 1229 248 1230 771 575
Fragments arranged by size:
1230 1229 771 575 502 318 248 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 760 792 1994 2026 2872 2902 2904
Size: 32 277 32 1202 32 846 30 2
Cuts at: 2904 2934 451
Size: 30 2547
Fragments arranged by size:
2547 1202 846 277 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2069 2069
Size: 5030
KasI G'GCGC_C
Cuts at: 1231 2460 2708 1231
Size: 1229 248 3553
Fragments arranged by size:
3553 1229 248
KpnI G_GTAC'C
Cuts at: 2050 2050
Size: 5030
MluI A'CGCG_T
Cuts at: 4463 4463
Size: 5030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 198 344 3789 3973 198
Size: 146 3445 184 1255
Fragments arranged by size:
3445 1255 184 146
MscI TGG'CCA
Cuts at: 289 2791 289
Size: 2502 2528
MslI CAynn'nnrTG
Cuts at: 487 3019 3146 487
Size: 2532 127 2371
Fragments arranged by size:
2532 2371 127
MspA1I CmG'CkG
Cuts at: 1237 1333 1973 2141 2455 2815 3599 3844 1237
Size: 96 640 168 314 360 784 245 2423
Fragments arranged by size:
2423 784 640 360 314 245 168 96
NaeI GCC'GGC
Cuts at: 786 3211 786
Size: 2425 2605
NarI GG'CG_CC
Cuts at: 1232 2461 2709 1232
Size: 1229 248 3553
Fragments arranged by size:
3553 1229 248
NcoI C'CATG_G
Cuts at: 473 473
Size: 5030
NgoMIV G'CCGG_C
Cuts at: 784 3209 784
Size: 2425 2605
NruI TCG'CGA
Cuts at: 1715 1715
Size: 5030
NspI r_CATG'y
Cuts at: 2083 2583 4186 2083
Size: 500 1603 2927
Fragments arranged by size:
2927 1603 500
PciI A'CATG_T
Cuts at: 4182 4182
Size: 5030
PfoI T'CCnGG_A
Cuts at: 1109 1109
Size: 5030
PmeI GTTT'AAAC
Cuts at: 2029 2029
Size: 5030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 3476
Size: 32 4998
PpuMI rG'GwC_Cy
Cuts at: 1052 1284 1527 2002 1052
Size: 232 243 475 4080
Fragments arranged by size:
4080 475 243 232
PshAI GACnn'nnGTC
Cuts at: 1637 1835 1637
Size: 198 4832
PspOMI G'GGCC_C
Cuts at: 877 877
Size: 5030
PstI C_TGCA'G
Cuts at: 1169 2077 2762 1169
Size: 908 685 3437
Fragments arranged by size:
3437 908 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5030
PvuII CAG'CTG
Cuts at: 1333 2455 2815 1333
Size: 1122 360 3548
Fragments arranged by size:
3548 1122 360
RsrII CG'GwC_CG
Cuts at: 3225 3225
Size: 5030
SacI G_AGCT'C
Cuts at: 943 1031 1913 2044 943
Size: 88 882 131 3929
Fragments arranged by size:
3929 882 131 88
SacII CC_GC'GG
Cuts at: 1238 1238
Size: 5030
SalI G'TCGA_C
Cuts at: 2067 2067
Size: 5030
SanDI GG'GwC_CC
Cuts at: 1052 1527 2002 1052
Size: 475 475 4080
Fragments arranged by size:
4080 475 475
SapI GCTCTTCn'nnn_
Cuts at: 3053 3263 3053
Size: 210 4820
SbfI CC_TGCA'GG
Cuts at: 2077 2077
Size: 5030
ScaI AGT'ACT
Cuts at: 4547 4547
Size: 5030
SexAI A'CCwGG_T
Cuts at: 281 281
Size: 5030
SfcI C'TryA_G
Cuts at: 33 1165 2073 2758 3726 3917 33
Size: 1132 908 685 968 191 1146
Fragments arranged by size:
1146 1132 968 908 685 191
SfoI GGC'GCC
Cuts at: 1233 2462 2710 1233
Size: 1229 248 3553
Fragments arranged by size:
3553 1229 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
SmaI CCC'GGG
Cuts at: 2052 2052
Size: 5030
SmlI C'TyrA_G
Cuts at: 2185 3100 3814 4076 2185
Size: 915 714 262 3139
Fragments arranged by size:
3139 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5030
SphI G_CATG'C
Cuts at: 2083 2583 2083
Size: 500 4530
StyI C'CwwG_G
Cuts at: 473 584 1466 1950 2164 2518 473
Size: 111 882 484 214 354 2985
Fragments arranged by size:
2985 882 484 354 214 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 808 1733 1877 2613 2746 3205 3414 808
Size: 925 144 736 133 459 209 2424
Fragments arranged by size:
2424 925 736 459 209 144 133
TatI w'GTAC_w
Cuts at: 428 4545 428
Size: 4117 913
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4547 4579 4547
Size: 32 4998
Tth111I GACn'n_nGTC
Cuts at: 1720 2827 1720
Size: 1107 3923
XbaI T'CTAG_A
Cuts at: 2061 2061
Size: 5030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 282 1737 1957 2016 2525 282
Size: 1455 220 59 509 2787
Fragments arranged by size:
2787 1455 509 220 59
XmaI C'CCGG_G
Cuts at: 2050 2050
Size: 5030
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BmtI BsaXI BsiWI
BsmI BsmBI BspEI BsrGI Bsu36I ClaI CspCI DraIII
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PflMI PmlI PsiI PspXI PsrI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI