Restriction map for
pF1KB3765
(Circular) (Six-base) MAPSORT of: pF1KB3765.seq Check: 3957 from: 1 to: 4514
pF1KB3765 4514 bp
With 182 enzymes: SgfI *
April 12, 2013 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 279 279
Size: 4514
AccI GT'mk_AC
Cuts at: 1552 1552
Size: 4514
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 677 4354 677
Size: 3677 837
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2771 3518 3786 2339
Size: 432 747 268 3067
Fragments arranged by size:
3067 747 432 268
AflIII A'CryG_T
Cuts at: 1866 2970 3947 1866
Size: 1104 977 2433
Fragments arranged by size:
2433 1104 977
AgeI A'CCGG_T
Cuts at: 2902 2902
Size: 4514
AlwNI CAG_nnn'CTG
Cuts at: 1819 3386 1819
Size: 1567 2947
ApaLI G'TGCA_C
Cuts at: 3284 3284
Size: 4514
ApoI r'AATT_y
Cuts at: 717 1041 1518 3867 4123 717
Size: 324 477 2349 256 1108
Fragments arranged by size:
2349 1108 477 324 256
AseI AT'TA_AT
Cuts at: 20 1351 20
Size: 1331 3183
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 1534 1534
Size: 4514
BamHI G'GATC_C
Cuts at: 1539 1539
Size: 4514
BanI G'GyrC_C
Cuts at: 340 1530 1944 2192 2227 340
Size: 1190 414 248 35 2627
Fragments arranged by size:
2627 1190 414 248 35
BanII G_rGCy'C
Cuts at: 495 1528 2558 495
Size: 1033 1030 2451
Fragments arranged by size:
2451 1033 1030
BbeI G_GCGC'C
Cuts at: 344 1948 2196 344
Size: 1604 248 2662
Fragments arranged by size:
2662 1604 248
BbsI GAAGACnn'nnnn_
Cuts at: 1229 1229
Size: 4514
BbvCI CC'TCA_GC
Cuts at: 1296 1296
Size: 4514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1194 1228 1194
Size: 34 4480
BciVI GTATCCnnnnn_n'
Cuts at: 1044 1219 2412 3173 4514 1044
Size: 175 1193 761 1341 1044
Fragments arranged by size:
1341 1193 1044 761 175
BclI T'GATC_A
Cuts at: 353 524 2034 353
Size: 171 1510 2833
Fragments arranged by size:
2833 1510 171
BglI GCCn_nnn'nGGC
Cuts at: 805 805
Size: 4514
BglII A'GATC_T
Cuts at: 2029 4066 2029
Size: 2037 2477
BlpI GC'TnA_GC
Cuts at: 1626 1626
Size: 4514
Bme1580I G_kGCm'C
Cuts at: 801 2139 2232 3288 801
Size: 1338 93 1056 2027
Fragments arranged by size:
2027 1338 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1726 1807 2142 4286 1726
Size: 81 335 2144 1954
Fragments arranged by size:
2144 1954 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 4514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 754
Size: 4514
Bpu10I CC'TnA_GC
Cuts at: 172 248 932 1296 172
Size: 76 684 364 3390
Fragments arranged by size:
3390 684 364 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 2569 3061 3359 1690
Size: 879 492 298 2845
Fragments arranged by size:
2845 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1004 4218 1004
Size: 3214 1300
BsaAI yAC'GTr
Cuts at: 187 1867 187
Size: 1680 2834
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 341 1945 2193 2895 4391 341
Size: 1604 248 702 1496 464
Fragments arranged by size:
1604 1496 702 464 248
BsaWI w'CCGG_w
Cuts at: 1703 2224 2902 3176 3323 3734 1703
Size: 521 678 274 147 411 2483
Fragments arranged by size:
2483 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 752 782 752
Size: 30 4484
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 963 334
Size: 629 3885
BseYI C'CCAG_C
Cuts at: 1939 2697 3274 3770 1939
Size: 758 577 496 2683
Fragments arranged by size:
2683 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 481 107
Size: 374 4140
BsiEI CG_ry'CG
Cuts at: 104 2056 2102 3310 104
Size: 1952 46 1208 1308
Fragments arranged by size:
1952 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 221 1528 2306 2496 3288 221
Size: 1307 778 190 792 1447
Fragments arranged by size:
1447 1307 792 778 190
BsmI GAATG_Cn'
Cuts at: 150 516 1489 150
Size: 366 973 3175
Fragments arranged by size:
3175 973 366
Bsp1286I G_dGCh'C
Cuts at: 221 495 801 1528 2139 2232 2306 2496 2558
Size: 274 306 727 611 93 74 190 62
Cuts at: 2558 3288 221
Size: 730 1447
Fragments arranged by size:
1447 730 727 611 306 274 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3734 3734
Size: 4514
BspHI T'CATG_A
Cuts at: 521 542 3690 521
Size: 21 3148 1345
Fragments arranged by size:
3148 1345 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 129 279 1564 1778 2080 2461 129
Size: 150 1285 214 302 381 2182
Fragments arranged by size:
2182 1285 381 302 214 150
BsrBI CCG'CTC
Cuts at: 50 2806 2860 4344 4509 50
Size: 2756 54 1484 165 55
Fragments arranged by size:
2756 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1399 2426 1399
Size: 1027 3487
BsrFI r'CCGG_y
Cuts at: 2512 2693 2902 2512
Size: 181 209 4124
Fragments arranged by size:
4124 209 181
BsrGI T'GTAC_A
Cuts at: 1464 1464
Size: 4514
BssSI C'ACGA_G
Cuts at: 1178 2785 3143 1178
Size: 1607 358 2549
Fragments arranged by size:
2549 1607 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1891 1891
Size: 4514
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstYI r'GATC_y
Cuts at: 1 1539 2006 2029 2364 2610 3622 3708 3720
Size: 1538 467 23 335 246 1012 86 12
Cuts at: 3720 4066 1
Size: 346 449
Fragments arranged by size:
1538 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1144 2464 2645 2808 1144
Size: 1320 181 163 2850
Fragments arranged by size:
2850 1320 181 163
BtsI GCAGTG_nn'
Cuts at: 1757 1757
Size: 4514
DraI TTT'AAA
Cuts at: 666 1513 666
Size: 847 3667
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3078 2220
Size: 858 3656
EaeI y'GGCC_r
Cuts at: 240 2099 2273 2664 2691 4036 240
Size: 1859 174 391 27 1345 718
Fragments arranged by size:
1859 1345 718 391 174 27
EagI C'GGCC_G
Cuts at: 2099 2099
Size: 4514
EarI CTCTTCn'nnn_
Cuts at: 1415 2537 2747 1415
Size: 1122 210 3182
Fragments arranged by size:
3182 1122 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2722 3032 3178 4324 2722
Size: 310 146 1146 2912
Fragments arranged by size:
2912 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1526 1526
Size: 4514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 2339 2771 3518 3786 754
Size: 1585 432 747 268 1482
Fragments arranged by size:
1585 1482 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1153 1653 1153
Size: 500 4014
EcoRI G'AATT_C
Cuts at: 1518 1518
Size: 4514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 778 925 2533 778
Size: 147 1608 2759
Fragments arranged by size:
2759 1608 147
FspI TGC'GCA
Cuts at: 2295 2295
Size: 4514
HaeII r_GCGC'y
Cuts at: 344 1948 2196 3218 4197 344
Size: 1604 248 1022 979 661
Fragments arranged by size:
1604 1022 979 661 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 1128 1160 1172 1204 2356 2386 2388
Size: 32 855 32 12 32 1152 30 2
Cuts at: 2388 2418 241
Size: 30 2337
Fragments arranged by size:
2337 1152 855 32 32 32 30 30
12 2
HincII GTy'rAC
Cuts at: 1553 1553
Size: 4514
KasI G'GCGC_C
Cuts at: 340 1944 2192 340
Size: 1604 248 2662
Fragments arranged by size:
2662 1604 248
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MluI A'CGCG_T
Cuts at: 3947 3947
Size: 4514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3185 3369 3185
Size: 184 4330
MscI TGG'CCA
Cuts at: 242 2275 242
Size: 2033 2481
MslI CAynn'nnrTG
Cuts at: 388 2503 2630 388
Size: 2115 127 2272
Fragments arranged by size:
2272 2115 127
MspA1I CmG'CkG
Cuts at: 1625 1939 2299 3312 3557 1625
Size: 314 360 1013 245 2582
Fragments arranged by size:
2582 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2695 2695
Size: 4514
NarI GG'CG_CC
Cuts at: 341 1945 2193 341
Size: 1604 248 2662
Fragments arranged by size:
2662 1604 248
NgoMIV G'CCGG_C
Cuts at: 2693 2693
Size: 4514
NspI r_CATG'y
Cuts at: 352 1567 2067 2974 352
Size: 1215 500 907 1892
Fragments arranged by size:
1892 1215 907 500
PasI CC'CwG_GG
Cuts at: 273 273
Size: 4514
PciI A'CATG_T
Cuts at: 2970 2970
Size: 4514
PflMI CCAn_nnn'nTGG
Cuts at: 749 749
Size: 4514
PfoI T'CCnGG_A
Cuts at: 1190 1190
Size: 4514
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4482
PstI C_TGCA'G
Cuts at: 1561 2246 1561
Size: 685 3829
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 1939 2299 1939
Size: 360 4154
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 1528 1528
Size: 4514
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 1415 2537 2747 1415
Size: 1122 210 3182
Fragments arranged by size:
3182 1122 210
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 4031 4031
Size: 4514
SfcI C'TryA_G
Cuts at: 33 1272 1557 2242 3235 3426 33
Size: 1239 285 685 993 191 1121
Fragments arranged by size:
1239 1121 993 685 285 191
SfoI GGC'GCC
Cuts at: 342 1946 2194 342
Size: 1604 248 2662
Fragments arranged by size:
2662 1604 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 1536 1536
Size: 4514
SmlI C'TyrA_G
Cuts at: 1669 2584 3076 3338 1669
Size: 915 492 262 2845
Fragments arranged by size:
2845 915 492 262
SnaBI TAC'GTA
Cuts at: 187 187
Size: 4514
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 1567 2067 1567
Size: 500 4014
StyI C'CwwG_G
Cuts at: 237 1648 2002 237
Size: 1411 354 2749
Fragments arranged by size:
2749 1411 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2097 2230 2689 2898 2097
Size: 133 459 209 3713
Fragments arranged by size:
3713 459 209 133
TatI w'GTAC_w
Cuts at: 1464 4029 1464
Size: 2565 1949
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 708 740 752 784 4031 4063 708
Size: 32 12 32 3247 32 1159
Fragments arranged by size:
3247 1159 32 32 32 12
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2009 2009
Size: 4514
XmaI C'CCGG_G
Cuts at: 1534 1534
Size: 4514
XmnI GAAnn'nnTTC
Cuts at: 738 738
Size: 4514
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
ZraI