Restriction map for
pF1KB3761
(Circular) (Six-base) MAPSORT of: pF1KB3761.seq Check: 1695 from: 1 to: 6317
pF1KB3761 6317 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 502 3355 502
Size: 2853 3464
Acc65I G'GTAC_C
Cuts at: 3333 3333
Size: 6317
AclI AA'CG_TT
Cuts at: 6157 6157
Size: 6317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 4142 4574 4927 5589 854
Size: 3288 432 353 662 1582
Fragments arranged by size:
3288 1582 662 432 353
AfeI AGC'GCT
Cuts at: 3108 3108
Size: 6317
AflIII A'CryG_T
Cuts at: 437 1568 2572 3240 3669 5469 5750 437
Size: 1131 1004 668 429 1800 281 1004
Fragments arranged by size:
1800 1131 1004 1004 668 429 281
AhdI GACnn_n'nnGTC
Cuts at: 1421 1421
Size: 6317
AlwNI CAG_nnn'CTG
Cuts at: 177 189 3622 5060 177
Size: 12 3433 1438 1434
Fragments arranged by size:
3433 1438 1434 12
ApaLI G'TGCA_C
Cuts at: 5155 5155
Size: 6317
ApoI r'AATT_y
Cuts at: 457 489 1502 2581 2902 3321 5670 5926 457
Size: 32 1013 1079 321 419 2349 256 848
Fragments arranged by size:
2349 1079 1013 848 419 321 256 32
AseI AT'TA_AT
Cuts at: 20 2380 20
Size: 2360 3957
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
AvaI C'yCGr_G
Cuts at: 3337 3337
Size: 6317
BamHI G'GATC_C
Cuts at: 3342 3342
Size: 6317
BanI G'GyrC_C
Cuts at: 3333 3747 3995 4030 3333
Size: 414 248 35 5620
Fragments arranged by size:
5620 414 248 35
BanII G_rGCy'C
Cuts at: 1748 3331 4361 1748
Size: 1583 1030 3704
Fragments arranged by size:
3704 1583 1030
BbeI G_GCGC'C
Cuts at: 3751 3999 3751
Size: 248 6069
BbsI GAAGACnn'nnnn_
Cuts at: 1557 1993 2088 1557
Size: 436 95 5786
Fragments arranged by size:
5786 436 95
BbvCI CC'TCA_GC
Cuts at: 131 131
Size: 6317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 217 251 3202 3236 217
Size: 34 2951 34 3298
Fragments arranged by size:
3298 2951 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4215 5271 6317 4215
Size: 1056 1046 4215
Fragments arranged by size:
4215 1056 1046
BclI T'GATC_A
Cuts at: 3837 3837
Size: 6317
BglI GCCn_nnn'nGGC
Cuts at: 163 163
Size: 6317
BglII A'GATC_T
Cuts at: 3832 5869 3832
Size: 2037 4280
BlpI GC'TnA_GC
Cuts at: 3429 3429
Size: 6317
Bme1580I G_kGCm'C
Cuts at: 757 3942 4035 5159 757
Size: 3185 93 1124 1915
Fragments arranged by size:
3185 1915 1124 93
BmgBI CAC'GTC
Cuts at: 2573 2573
Size: 6317
BmrI ACTGGGnnnn_n'
Cuts at: 2227 2471 3529 3610 3945 6089 2227
Size: 244 1058 81 335 2144 2455
Fragments arranged by size:
2455 2144 1058 335 244 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 988 1764 4684 988
Size: 776 2920 2621
Fragments arranged by size:
2920 2621 776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1825 2315 1825
Size: 490 5827
Bpu10I CC'TnA_GC
Cuts at: 131 2469 131
Size: 2338 3979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1734 3493 4372 5086 5384 1734
Size: 1759 879 714 298 2667
Fragments arranged by size:
2667 1759 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 6021 6021
Size: 6317
BsaAI yAC'GTr
Cuts at: 2518 2739 3670 2518
Size: 221 931 5165
Fragments arranged by size:
5165 931 221
BsaBI GATnn'nnATC
Cuts at: 6 3831 5868 6
Size: 3825 2037 455
Fragments arranged by size:
3825 2037 455
BsaHI Gr'CG_yC
Cuts at: 3748 3996 4698 6194 3748
Size: 248 702 1496 3871
Fragments arranged by size:
3871 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1222 3506 4027 4705 5116 5263 5537 1222
Size: 2284 521 678 411 147 274 2002
Fragments arranged by size:
2284 2002 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1904 1934 1904
Size: 30 6287
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 612 1943 2626 612
Size: 1331 683 4303
Fragments arranged by size:
4303 1331 683
BseYI C'CCAG_C
Cuts at: 3047 3742 4500 5165 5573 3047
Size: 695 758 665 408 3791
Fragments arranged by size:
3791 758 695 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1808 1942 1808
Size: 134 6183
BsiEI CG_ry'CG
Cuts at: 104 215 3859 3905 5135 104
Size: 111 3644 46 1230 1286
Fragments arranged by size:
3644 1286 1230 111 46
BsiHKAI G_wGCw'C
Cuts at: 914 1637 3331 4109 4299 5159 914
Size: 723 1694 778 190 860 2072
Fragments arranged by size:
2072 1694 860 778 723 190
BsmI GAATG_Cn'
Cuts at: 973 973
Size: 6317
Bsp1286I G_dGCh'C
Cuts at: 757 914 1637 1748 3331 3942 4035 4109 4299
Size: 157 723 111 1583 611 93 74 190
Cuts at: 4299 4361 5159 757
Size: 62 798 1915
Fragments arranged by size:
1915 1583 798 723 611 190 157 111
93 74 62
BspHI T'CATG_A
Cuts at: 4749 4749
Size: 6317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3367 3581 3883 4264 3367
Size: 214 302 381 5420
Fragments arranged by size:
5420 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1733 4609 4663 6147 6312 50
Size: 1683 2876 54 1484 165 55
Fragments arranged by size:
2876 1683 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 851 4229 851
Size: 3378 2939
BsrFI r'CCGG_y
Cuts at: 1292 4315 4496 1292
Size: 3023 181 3113
Fragments arranged by size:
3113 3023 181
BsrGI T'GTAC_A
Cuts at: 3116 3116
Size: 6317
BssSI C'ACGA_G
Cuts at: 1118 4588 5296 1118
Size: 3470 708 2139
Fragments arranged by size:
3470 2139 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2071 2290 3694 2071
Size: 219 1404 4694
Fragments arranged by size:
4694 1404 219
BstBI TT'CG_AA
Cuts at: 1983 2004 4678 1983
Size: 21 2674 3622
Fragments arranged by size:
3622 2674 21
BstYI r'GATC_y
Cuts at: 1 621 726 1064 1459 3342 3809 3832 4167
Size: 620 105 338 395 1883 467 23 335
Cuts at: 4167 4413 4719 4731 4817 5869 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1883 1052 620 467 449 395 338 335
306 246 105 86 23 12
BtgI C'CryG_G
Cuts at: 326 326
Size: 6317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4267 4448 4611 4267
Size: 181 163 5973
Fragments arranged by size:
5973 181 163
BtsI GCAGTG_nn'
Cuts at: 618 1016 3560 618
Size: 398 2544 3375
Fragments arranged by size:
3375 2544 398
ClaI AT'CG_AT
Cuts at: 3212 3212
Size: 6317
DraI TTT'AAA
Cuts at: 789 3316 789
Size: 2527 3790
DraIII CAC_nnn'GTG
Cuts at: 1333 1333
Size: 6317
DrdI GACnn_nn'nnGTC
Cuts at: 4023 5367 4023
Size: 1344 4973
EaeI y'GGCC_r
Cuts at: 212 2222 3902 4076 4467 4494 5839 212
Size: 2010 1680 174 391 27 1345 690
Fragments arranged by size:
2010 1680 1345 690 391 174 27
EagI C'GGCC_G
Cuts at: 212 3902 212
Size: 3690 2627
EarI CTCTTCn'nnn_
Cuts at: 830 1022 1082 1733 2001 2216 2922 4340 4550
Size: 192 60 651 268 215 706 1418 210
Cuts at: 4550 830
Size: 2597
Fragments arranged by size:
2597 1418 706 651 268 215 210 192
60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4525 5267 5413 6127 4525
Size: 742 146 714 4715
Fragments arranged by size:
4715 742 714 146
EcoICRI GAG'CTC
Cuts at: 3329 3329
Size: 6317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 1825 2315 4142 4574 4927 5589 854
Size: 971 490 1827 432 353 662 1582
Fragments arranged by size:
1827 1582 971 662 490 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 181 199 2063 181
Size: 18 1864 4435
Fragments arranged by size:
4435 1864 18
EcoO109I rG'GnC_Cy
Cuts at: 174 2097 2465 3456 174
Size: 1923 368 991 3035
Fragments arranged by size:
3035 1923 991 368
EcoRI G'AATT_C
Cuts at: 2581 3321 2581
Size: 740 5577
EcoRV GAT'ATC
Cuts at: 3156 3156
Size: 6317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4336 4336
Size: 6317
FspI TGC'GCA
Cuts at: 4098 4098
Size: 6317
HaeII r_GCGC'y
Cuts at: 169 3110 3751 3999 5229 6000 169
Size: 2941 641 248 1230 771 486
Fragments arranged by size:
2941 1230 771 641 486 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1407 1439 2970 3002 3211 3243 4159 4189 4191
Size: 32 1531 32 209 32 916 30 2
Cuts at: 4191 4221 1407
Size: 30 3503
Fragments arranged by size:
3503 1531 916 209 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1426 3356 1426
Size: 1930 4387
KasI G'GCGC_C
Cuts at: 3747 3995 3747
Size: 248 6069
KpnI G_GTAC'C
Cuts at: 3337 3337
Size: 6317
MluI A'CGCG_T
Cuts at: 3240 5750 3240
Size: 2510 3807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 312 619 5076 5260 312
Size: 307 4457 184 1369
Fragments arranged by size:
4457 1369 307 184
MscI TGG'CCA
Cuts at: 2224 4078 2224
Size: 1854 4463
MslI CAynn'nnrTG
Cuts at: 3080 4306 4433 3080
Size: 1226 127 4964
Fragments arranged by size:
4964 1226 127
MspA1I CmG'CkG
Cuts at: 3428 3742 4102 4886 5131 3428
Size: 314 360 784 245 4614
Fragments arranged by size:
4614 784 360 314 245
NaeI GCC'GGC
Cuts at: 4498 4498
Size: 6317
NarI GG'CG_CC
Cuts at: 3748 3996 3748
Size: 248 6069
NdeI CA'TA_TG
Cuts at: 558 2975 558
Size: 2417 3900
NgoMIV G'CCGG_C
Cuts at: 4496 4496
Size: 6317
NspI r_CATG'y
Cuts at: 290 308 441 1572 3370 3870 5473 290
Size: 18 133 1131 1798 500 1603 1134
Fragments arranged by size:
1798 1603 1134 1131 500 133 18
PciI A'CATG_T
Cuts at: 437 1568 5469 437
Size: 1131 3901 1285
Fragments arranged by size:
3901 1285 1131
PflMI CCAn_nnn'nTGG
Cuts at: 2275 2979 2275
Size: 704 5613
PfoI T'CCnGG_A
Cuts at: 974 974
Size: 6317
PmeI GTTT'AAAC
Cuts at: 3316 3316
Size: 6317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4763 4795 4763
Size: 32 6285
PpuMI rG'GwC_Cy
Cuts at: 2097 2465 2097
Size: 368 5949
PsiI TTA'TAA
Cuts at: 1345 1514 1709 2899 1345
Size: 169 195 1190 4763
Fragments arranged by size:
4763 1190 195 169
PstI C_TGCA'G
Cuts at: 3364 4049 3364
Size: 685 5632
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6317
PvuII CAG'CTG
Cuts at: 3742 4102 3742
Size: 360 5957
RsrII CG'GwC_CG
Cuts at: 4512 4512
Size: 6317
SacI G_AGCT'C
Cuts at: 3331 3331
Size: 6317
SalI G'TCGA_C
Cuts at: 3354 3354
Size: 6317
SapI GCTCTTCn'nnn_
Cuts at: 830 4340 4550 830
Size: 3510 210 2597
Fragments arranged by size:
3510 2597 210
SbfI CC_TGCA'GG
Cuts at: 3364 3364
Size: 6317
ScaI AGT'ACT
Cuts at: 5834 5834
Size: 6317
SexAI A'CCwGG_T
Cuts at: 2199 2199
Size: 6317
SfcI C'TryA_G
Cuts at: 33 169 252 1644 2049 3280 3360 4045 5013
Size: 136 83 1392 405 1231 80 685 968
Cuts at: 5013 5204 33
Size: 191 1146
Fragments arranged by size:
1392 1231 1146 968 685 405 191 136
83 80
SfoI GGC'GCC
Cuts at: 3749 3997 3749
Size: 248 6069
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
SmaI CCC'GGG
Cuts at: 3339 3339
Size: 6317
SmlI C'TyrA_G
Cuts at: 1749 3472 4387 5101 5363 1749
Size: 1723 915 714 262 2703
Fragments arranged by size:
2703 1723 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6317
SphI G_CATG'C
Cuts at: 308 3370 3870 308
Size: 3062 500 2755
Fragments arranged by size:
3062 2755 500
SspI AAT'ATT
Cuts at: 512 1350 512
Size: 838 5479
StuI AGG'CCT
Cuts at: 3092 3092
Size: 6317
StyI C'CwwG_G
Cuts at: 1666 3451 3805 1666
Size: 1785 354 4178
Fragments arranged by size:
4178 1785 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1294 1979 3900 4033 4492 4701 1294
Size: 685 1921 133 459 209 2910
Fragments arranged by size:
2910 1921 685 459 209 133
TatI w'GTAC_w
Cuts at: 858 3116 5832 858
Size: 2258 2716 1343
Fragments arranged by size:
2716 2258 1343
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1904 1936 5834 5866 1904
Size: 32 3898 32 2355
Fragments arranged by size:
3898 2355 32 32
Tth111I GACn'n_nGTC
Cuts at: 4114 4114
Size: 6317
XbaI T'CTAG_A
Cuts at: 3348 3348
Size: 6317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3812 3812
Size: 6317
XmaI C'CCGG_G
Cuts at: 3337 3337
Size: 6317
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmtI BsiWI BsmBI BspEI
BssHII BstEII BstXI BstZ17I Bsu36I CspCI FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI