Restriction map for
pF1KB3754
(Circular) (Six-base) MAPSORT of: pF1KB3754.seq Check: 7924 from: 1 to: 5162
pF1KB3754 5162 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 999 999
Size: 5162
AccI GT'mk_AC
Cuts at: 2200 2200
Size: 5162
Acc65I G'GTAC_C
Cuts at: 2178 2178
Size: 5162
AclI AA'CG_TT
Cuts at: 5002 5002
Size: 5162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2987 3419 3772 4434 2063
Size: 924 432 353 662 2791
Fragments arranged by size:
2791 924 662 432 353
AflII C'TTAA_G
Cuts at: 1764 1764
Size: 5162
AflIII A'CryG_T
Cuts at: 338 974 2514 4314 4595 338
Size: 636 1540 1800 281 905
Fragments arranged by size:
1800 1540 905 636 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1316 1370 1964 1316
Size: 54 594 4514
Fragments arranged by size:
4514 594 54
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1469 1501 1469
Size: 32 5130
AlwNI CAG_nnn'CTG
Cuts at: 695 771 2121 2467 3905 695
Size: 76 1350 346 1438 1952
Fragments arranged by size:
1952 1438 1350 346 76
ApaLI G'TGCA_C
Cuts at: 4000 4000
Size: 5162
ApoI r'AATT_y
Cuts at: 485 1833 1876 2166 4515 4771 485
Size: 1348 43 290 2349 256 876
Fragments arranged by size:
2349 1348 876 290 256 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 5162
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
AvaI C'yCGr_G
Cuts at: 2182 2182
Size: 5162
BamHI G'GATC_C
Cuts at: 2187 2187
Size: 5162
BanI G'GyrC_C
Cuts at: 2178 2592 2840 2875 2178
Size: 414 248 35 4465
Fragments arranged by size:
4465 414 248 35
BanII G_rGCy'C
Cuts at: 180 2093 2176 3206 180
Size: 1913 83 1030 2136
Fragments arranged by size:
2136 1913 1030 83
BbeI G_GCGC'C
Cuts at: 2596 2844 2596
Size: 248 4914
BbsI GAAGACnn'nnnn_
Cuts at: 159 810 159
Size: 651 4511
BbvCI CC'TCA_GC
Cuts at: 1289 1289
Size: 5162
BciVI GTATCCnnnnn_n'
Cuts at: 3060 4116 5162 3060
Size: 1056 1046 3060
Fragments arranged by size:
3060 1056 1046
BclI T'GATC_A
Cuts at: 2682 2682
Size: 5162
BfrBI ATG'CAT
Cuts at: 1012 1012
Size: 5162
BglII A'GATC_T
Cuts at: 183 2677 4714 183
Size: 2494 2037 631
Fragments arranged by size:
2494 2037 631
BlpI GC'TnA_GC
Cuts at: 1358 2274 1358
Size: 916 4246
Bme1580I G_kGCm'C
Cuts at: 2787 2880 4004 2787
Size: 93 1124 3945
Fragments arranged by size:
3945 1124 93
BmgBI CAC'GTC
Cuts at: 977 977
Size: 5162
BmrI ACTGGGnnnn_n'
Cuts at: 2374 2455 2790 4934 2374
Size: 81 335 2144 2602
Fragments arranged by size:
2602 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1319 3529 1319
Size: 2210 2952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 942 1516 2077 2101 564
Size: 378 574 561 24 3625
Fragments arranged by size:
3625 574 561 378 24
Bpu10I CC'TnA_GC
Cuts at: 229 1027 1289 229
Size: 798 262 4102
Fragments arranged by size:
4102 798 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 1219 2338 3217 3931 4229 164
Size: 1055 1119 879 714 298 1097
Fragments arranged by size:
1119 1097 1055 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1576 4866 1576
Size: 3290 1872
BsaAI yAC'GTr
Cuts at: 2515 2515
Size: 5162
BsaBI GATnn'nnATC
Cuts at: 6 188 2024 2676 4713 6
Size: 182 1836 652 2037 455
Fragments arranged by size:
2037 1836 652 455 182
BsaHI Gr'CG_yC
Cuts at: 2593 2841 3543 5039 2593
Size: 248 702 1496 2716
Fragments arranged by size:
2716 1496 702 248
BsaWI w'CCGG_w
Cuts at: 468 825 1658 2351 2872 3550 3961 4108 4382
Size: 357 833 693 521 678 411 147 274
Cuts at: 4382 468
Size: 1248
Fragments arranged by size:
1248 833 693 678 521 411 357 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1469 1499 1469
Size: 30 5132
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 1155 1477 1605 427
Size: 728 322 128 3984
Fragments arranged by size:
3984 728 322 128
BseYI C'CCAG_C
Cuts at: 1621 2587 3345 4010 4418 1621
Size: 966 758 665 408 2365
Fragments arranged by size:
2365 966 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2056 2056
Size: 5162
BsiEI CG_ry'CG
Cuts at: 104 2704 2750 3980 104
Size: 2600 46 1230 1286
Fragments arranged by size:
2600 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 362 1305 2093 2176 2954 3144 4004 362
Size: 943 788 83 778 190 860 1520
Fragments arranged by size:
1520 943 860 788 778 190 83
BsmBI CGTCTCn'nnnn_
Cuts at: 1283 1283
Size: 5162
Bsp1286I G_dGCh'C
Cuts at: 180 362 1305 2093 2176 2787 2880 2954 3144
Size: 182 943 788 83 611 93 74 190
Cuts at: 3144 3206 4004 180
Size: 62 798 1338
Fragments arranged by size:
1338 943 798 788 611 190 182 93
83 74 62
BspEI T'CCGG_A
Cuts at: 468 468
Size: 5162
BspHI T'CATG_A
Cuts at: 1574 3594 1574
Size: 2020 3142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 999 2212 2426 2728 3109 999
Size: 1213 214 302 381 3052
Fragments arranged by size:
3052 1213 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1613 3454 3508 4992 5157 50
Size: 1563 1841 54 1484 165 55
Fragments arranged by size:
1841 1563 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 289 3074 289
Size: 2785 2377
BsrFI r'CCGG_y
Cuts at: 934 1211 3160 3341 934
Size: 277 1949 181 2755
Fragments arranged by size:
2755 1949 277 181
BsrGI T'GTAC_A
Cuts at: 1728 1728
Size: 5162
BssSI C'ACGA_G
Cuts at: 3433 4141 3433
Size: 708 4454
BstAPI GCAn_nnn'nTGC
Cuts at: 2539 2539
Size: 5162
BstBI TT'CG_AA
Cuts at: 3523 3523
Size: 5162
BstXI CCAn_nnnn'nTGG
Cuts at: 1470 1470
Size: 5162
BstYI r'GATC_y
Cuts at: 1 183 691 753 1394 1899 2025 2187 2654
Size: 182 508 62 641 505 126 162 467
Cuts at: 2654 2677 3012 3258 3564 3576 3662 4714 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 641 508 505 467 449 335 306
246 182 162 126 86 62 23 12
Bsu36I CC'TnA_GG
Cuts at: 1167 1167
Size: 5162
BtgI C'CryG_G
Cuts at: 140 477 641 140
Size: 337 164 4661
Fragments arranged by size:
4661 337 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3112 3293 3456 3112
Size: 181 163 4818
Fragments arranged by size:
4818 181 163
BtsI GCAGTG_nn'
Cuts at: 772 2405 772
Size: 1633 3529
ClaI AT'CG_AT
Cuts at: 1325 1325
Size: 5162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1076 1111 1076
Size: 35 5127
DraI TTT'AAA
Cuts at: 2161 2161
Size: 5162
DraIII CAC_nnn'GTG
Cuts at: 264 524 1107 264
Size: 260 583 4319
Fragments arranged by size:
4319 583 260
DrdI GACnn_nn'nnGTC
Cuts at: 2868 4212 2868
Size: 1344 3818
EaeI y'GGCC_r
Cuts at: 2747 2921 3312 3339 4684 2747
Size: 174 391 27 1345 3225
Fragments arranged by size:
3225 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2747 2747
Size: 5162
EarI CTCTTCn'nnn_
Cuts at: 791 1278 3185 3395 791
Size: 487 1907 210 2558
Fragments arranged by size:
2558 1907 487 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3370 4112 4258 4972 3370
Size: 742 146 714 3560
Fragments arranged by size:
3560 742 714 146
EcoICRI GAG'CTC
Cuts at: 2091 2174 2091
Size: 83 5079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 942 1516 2063 2077 2101 2987 3419 3772
Size: 378 574 547 14 24 886 432 353
Cuts at: 3772 4434 564
Size: 662 1292
Fragments arranged by size:
1292 886 662 574 547 432 378 353
24 14
EcoO109I rG'GnC_Cy
Cuts at: 658 2301 658
Size: 1643 3519
EcoRI G'AATT_C
Cuts at: 1833 2166 1833
Size: 333 4829
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 5162
FspI TGC'GCA
Cuts at: 2943 2943
Size: 5162
HaeII r_GCGC'y
Cuts at: 2596 2844 4074 4845 2596
Size: 248 1230 771 2913
Fragments arranged by size:
2913 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 162 194 521 553 961 993 3004 3034 3036
Size: 32 327 32 408 32 2011 30 2
Cuts at: 3036 3066 162
Size: 30 2258
Fragments arranged by size:
2258 2011 408 327 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2201 2201
Size: 5162
HindIII A'AGCT_T
Cuts at: 226 1424 2132 226
Size: 1198 708 3256
Fragments arranged by size:
3256 1198 708
KasI G'GCGC_C
Cuts at: 2592 2840 2592
Size: 248 4914
KpnI G_GTAC'C
Cuts at: 2182 2182
Size: 5162
MluI A'CGCG_T
Cuts at: 4595 4595
Size: 5162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 222 1544 3921 4105 222
Size: 1322 2377 184 1279
Fragments arranged by size:
2377 1322 1279 184
MscI TGG'CCA
Cuts at: 2923 2923
Size: 5162
MslI CAynn'nnrTG
Cuts at: 745 935 3151 3278 745
Size: 190 2216 127 2629
Fragments arranged by size:
2629 2216 190 127
MspA1I CmG'CkG
Cuts at: 159 1350 2079 2273 2587 2947 3731 3976 159
Size: 1191 729 194 314 360 784 245 1345
Fragments arranged by size:
1345 1191 784 729 360 314 245 194
NaeI GCC'GGC
Cuts at: 3343 3343
Size: 5162
NarI GG'CG_CC
Cuts at: 2593 2841 2593
Size: 248 4914
NcoI C'CATG_G
Cuts at: 140 477 140
Size: 337 4825
NdeI CA'TA_TG
Cuts at: 271 271
Size: 5162
NgoMIV G'CCGG_C
Cuts at: 3341 3341
Size: 5162
NsiI A_TGCA'T
Cuts at: 1014 1014
Size: 5162
NspI r_CATG'y
Cuts at: 224 934 1197 2215 2715 4318 224
Size: 710 263 1018 500 1603 1068
Fragments arranged by size:
1603 1068 1018 710 500 263
PciI A'CATG_T
Cuts at: 4314 4314
Size: 5162
PfoI T'CCnGG_A
Cuts at: 1532 1532
Size: 5162
PmeI GTTT'AAAC
Cuts at: 2161 2161
Size: 5162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1469 1501 3608 3640 1469
Size: 32 2107 32 2991
Fragments arranged by size:
2991 2107 32 32
PpuMI rG'GwC_Cy
Cuts at: 658 658
Size: 5162
PstI C_TGCA'G
Cuts at: 1791 2209 2894 1791
Size: 418 685 4059
Fragments arranged by size:
4059 685 418
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5162
PvuII CAG'CTG
Cuts at: 159 1350 2079 2587 2947 159
Size: 1191 729 508 360 2374
Fragments arranged by size:
2374 1191 729 508 360
RsrII CG'GwC_CG
Cuts at: 3357 3357
Size: 5162
SacI G_AGCT'C
Cuts at: 2093 2176 2093
Size: 83 5079
SalI G'TCGA_C
Cuts at: 2199 2199
Size: 5162
SapI GCTCTTCn'nnn_
Cuts at: 3185 3395 3185
Size: 210 4952
SbfI CC_TGCA'GG
Cuts at: 2209 2209
Size: 5162
ScaI AGT'ACT
Cuts at: 4679 4679
Size: 5162
SexAI A'CCwGG_T
Cuts at: 260 260
Size: 5162
SfcI C'TryA_G
Cuts at: 33 210 1787 2205 2890 3858 4049 33
Size: 177 1577 418 685 968 191 1146
Fragments arranged by size:
1577 1146 968 685 418 191 177
SfoI GGC'GCC
Cuts at: 2594 2842 2594
Size: 248 4914
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
SmaI CCC'GGG
Cuts at: 2184 2184
Size: 5162
SmlI C'TyrA_G
Cuts at: 179 1198 1764 2317 3232 3946 4208 179
Size: 1019 566 553 915 714 262 1133
Fragments arranged by size:
1133 1019 915 714 566 553 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5162
SphI G_CATG'C
Cuts at: 224 1197 2215 2715 224
Size: 973 1018 500 2671
Fragments arranged by size:
2671 1018 973 500
StyI C'CwwG_G
Cuts at: 140 477 2296 2650 140
Size: 337 1819 354 2652
Fragments arranged by size:
2652 1819 354 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2745 2878 3337 3546 2745
Size: 133 459 209 4361
Fragments arranged by size:
4361 459 209 133
TatI w'GTAC_w
Cuts at: 971 1728 4677 971
Size: 757 2949 1456
Fragments arranged by size:
2949 1456 757
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4679 4711 4679
Size: 32 5130
Tth111I GACn'n_nGTC
Cuts at: 2068 2959 2068
Size: 891 4271
XbaI T'CTAG_A
Cuts at: 2193 2193
Size: 5162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 588 2657 588
Size: 2069 3093
XmaI C'CCGG_G
Cuts at: 2182 2182
Size: 5162
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI ApaI AscI AvrII
BaeI BcgI BglI BmtI BsiWI BsmI BssHII BstEII
BstZ17I EcoNI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI