Restriction map for
pF1KB3746
(Circular) (Six-base) MAPSORT of: pF1KB3746.seq Check: 8519 from: 1 to: 4139
pF1KB3746 4139 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1177 1177
Size: 4139
Acc65I G'GTAC_C
Cuts at: 1155 1155
Size: 4139
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 968 1964 2396 3143 3411 653
Size: 315 996 432 747 268 1381
Fragments arranged by size:
1381 996 747 432 315 268
AflIII A'CryG_T
Cuts at: 1491 2595 3572 1491
Size: 1104 977 2058
Fragments arranged by size:
2058 1104 977
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AlwNI CAG_nnn'CTG
Cuts at: 527 1053 1444 3011 527
Size: 526 391 1567 1655
Fragments arranged by size:
1655 1567 526 391
ApaI G_GGCC'C
Cuts at: 393 393
Size: 4139
ApaLI G'TGCA_C
Cuts at: 2909 2909
Size: 4139
ApoI r'AATT_y
Cuts at: 1143 3492 3748 1143
Size: 2349 256 1534
Fragments arranged by size:
2349 1534 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 236 1060 1159 236
Size: 824 99 3216
Fragments arranged by size:
3216 824 99
BamHI G'GATC_C
Cuts at: 1164 1164
Size: 4139
BanI G'GyrC_C
Cuts at: 1155 1569 1817 1852 1155
Size: 414 248 35 3442
Fragments arranged by size:
3442 414 248 35
BanII G_rGCy'C
Cuts at: 369 393 1153 2183 369
Size: 24 760 1030 2325
Fragments arranged by size:
2325 1030 760 24
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BbsI GAAGACnn'nnnn_
Cuts at: 224 435 995 224
Size: 211 560 3368
Fragments arranged by size:
3368 560 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 271 305 315 349 271
Size: 34 10 34 4061
Fragments arranged by size:
4061 34 34 10
BciVI GTATCCnnnnn_n'
Cuts at: 554 2037 2798 4139 554
Size: 1483 761 1341 554
Fragments arranged by size:
1483 1341 761 554
BclI T'GATC_A
Cuts at: 1659 1659
Size: 4139
BglII A'GATC_T
Cuts at: 1654 3691 1654
Size: 2037 2102
BlpI GC'TnA_GC
Cuts at: 1251 1251
Size: 4139
Bme1580I G_kGCm'C
Cuts at: 393 937 1764 1857 2913 393
Size: 544 827 93 1056 1619
Fragments arranged by size:
1619 1056 827 544 93
BmrI ACTGGGnnnn_n'
Cuts at: 1351 1432 1767 3911 1351
Size: 81 335 2144 1579
Fragments arranged by size:
2144 1579 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 727
Size: 4139
Bpu10I CC'TnA_GC
Cuts at: 185 322 185
Size: 137 4002
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 2194 2686 2984 1315
Size: 879 492 298 2470
Fragments arranged by size:
2470 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3843 3843
Size: 4139
BsaAI yAC'GTr
Cuts at: 1492 1492
Size: 4139
BsaBI GATnn'nnATC
Cuts at: 6 736 1653 3690 6
Size: 730 917 2037 455
Fragments arranged by size:
2037 917 730 455
BsaHI Gr'CG_yC
Cuts at: 1570 1818 2520 4016 1570
Size: 248 702 1496 1693
Fragments arranged by size:
1693 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1328 1849 2527 2801 2948 3359 1328
Size: 521 678 274 147 411 2108
Fragments arranged by size:
2108 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 391
Size: 4139
BseYI C'CCAG_C
Cuts at: 940 1564 2322 2899 3395 940
Size: 624 758 577 496 1684
Fragments arranged by size:
1684 758 624 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 4139
BsiEI CG_ry'CG
Cuts at: 104 387 1681 1727 2935 104
Size: 283 1294 46 1208 1308
Fragments arranged by size:
1308 1294 1208 283 46
BsiHKAI G_wGCw'C
Cuts at: 208 328 1153 1931 2121 2913 208
Size: 120 825 778 190 792 1434
Fragments arranged by size:
1434 825 792 778 190 120
Bsp1286I G_dGCh'C
Cuts at: 208 328 369 393 937 1153 1764 1857 1931
Size: 120 41 24 544 216 611 93 74
Cuts at: 1931 2121 2183 2913 208
Size: 190 62 730 1434
Fragments arranged by size:
1434 730 611 544 216 190 120 93
74 62 41 24
BspEI T'CCGG_A
Cuts at: 3359 3359
Size: 4139
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1189 1403 1705 2086 1189
Size: 214 302 381 3242
Fragments arranged by size:
3242 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2431 2485 3969 4134 50
Size: 2381 54 1484 165 55
Fragments arranged by size:
2381 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2051 2051
Size: 4139
BsrFI r'CCGG_y
Cuts at: 2137 2318 2527 2137
Size: 181 209 3749
Fragments arranged by size:
3749 209 181
BssSI C'ACGA_G
Cuts at: 2410 2768 2410
Size: 358 3781
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 1516
Size: 4139
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstEII G'GTnAC_C
Cuts at: 225 225
Size: 4139
BstXI CCAn_nnnn'nTGG
Cuts at: 557 649 557
Size: 92 4047
BstYI r'GATC_y
Cuts at: 1 1164 1631 1654 1989 2235 3247 3333 3345
Size: 1163 467 23 335 246 1012 86 12
Cuts at: 3345 3691 1
Size: 346 449
Fragments arranged by size:
1163 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 867 867
Size: 4139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2089 2270 2433 2089
Size: 181 163 3795
Fragments arranged by size:
3795 181 163
BtsI GCAGTG_nn'
Cuts at: 953 1382 953
Size: 429 3710
DraI TTT'AAA
Cuts at: 804 1138 804
Size: 334 3805
DraIII CAC_nnn'GTG
Cuts at: 1087 1087
Size: 4139
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 1724 1898 2289 2316 3661 1724
Size: 174 391 27 1345 2202
Fragments arranged by size:
2202 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 236 2162 2372 236
Size: 1926 210 2003
Fragments arranged by size:
2003 1926 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 535 2347 2657 2803 3949 535
Size: 1812 310 146 1146 725
Fragments arranged by size:
1812 1146 725 310 146
EcoICRI GAG'CTC
Cuts at: 1151 1151
Size: 4139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 727 968 1964 2396 3143 3411 653
Size: 74 241 996 432 747 268 1381
Fragments arranged by size:
1381 996 747 432 268 241 74
EcoNI CCTnn'n_nnAGG
Cuts at: 919 919
Size: 4139
EcoO109I rG'GnC_Cy
Cuts at: 285 390 1065 1278 285
Size: 105 675 213 3146
Fragments arranged by size:
3146 675 213 105
EcoRI G'AATT_C
Cuts at: 1143 1143
Size: 4139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 496 2158 496
Size: 1662 2477
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 1573 1821 2843 3822 1573
Size: 248 1022 979 1890
Fragments arranged by size:
1890 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 848 868 880 900 1981 2011 2013 2043 848
Size: 20 12 20 1081 30 2 30 2944
Fragments arranged by size:
2944 1081 30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 408 1178 408
Size: 770 3369
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 1159 1159
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2810 2994 2810
Size: 184 3955
MscI TGG'CCA
Cuts at: 1900 1900
Size: 4139
MslI CAynn'nnrTG
Cuts at: 2128 2255 2128
Size: 127 4012
MspA1I CmG'CkG
Cuts at: 419 1119 1250 1564 1924 2937 3182 419
Size: 700 131 314 360 1013 245 1376
Fragments arranged by size:
1376 1013 700 360 314 245 131
NaeI GCC'GGC
Cuts at: 2320 2320
Size: 4139
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NcoI C'CATG_G
Cuts at: 867 867
Size: 4139
NgoMIV G'CCGG_C
Cuts at: 2318 2318
Size: 4139
NspI r_CATG'y
Cuts at: 1192 1692 2599 1192
Size: 500 907 2732
Fragments arranged by size:
2732 907 500
PasI CC'CwG_GG
Cuts at: 521 521
Size: 4139
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PfoI T'CCnGG_A
Cuts at: 743 743
Size: 4139
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PspOMI G'GGCC_C
Cuts at: 389 389
Size: 4139
PstI C_TGCA'G
Cuts at: 251 398 1186 1871 251
Size: 147 788 685 2519
Fragments arranged by size:
2519 788 685 147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 419 1119 1564 1924 419
Size: 700 445 360 2634
Fragments arranged by size:
2634 700 445 360
RsrII CG'GwC_CG
Cuts at: 2334 2334
Size: 4139
SacI G_AGCT'C
Cuts at: 1153 1153
Size: 4139
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 236 2162 2372 236
Size: 1926 210 2003
Fragments arranged by size:
2003 1926 210
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SexAI A'CCwGG_T
Cuts at: 221 221
Size: 4139
SfcI C'TryA_G
Cuts at: 33 247 394 420 1182 1867 2860 3051 33
Size: 214 147 26 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 214 191 147 26
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 238 1161 238
Size: 923 3216
SmlI C'TyrA_G
Cuts at: 1294 2209 2701 2963 1294
Size: 915 492 262 2470
Fragments arranged by size:
2470 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 1192 1692 1192
Size: 500 3639
SspI AAT'ATT
Cuts at: 800 800
Size: 4139
StyI C'CwwG_G
Cuts at: 867 920 1273 1627 867
Size: 53 353 354 3379
Fragments arranged by size:
3379 354 353 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1722 1855 2314 2523 1722
Size: 133 459 209 3338
Fragments arranged by size:
3338 459 209 133
TatI w'GTAC_w
Cuts at: 1121 3654 1121
Size: 2533 1606
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 885 917 3656 3688 885
Size: 32 2739 32 1336
Fragments arranged by size:
2739 1336 32 32
Tth111I GACn'n_nGTC
Cuts at: 223 1936 223
Size: 1713 2426
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 144 1634 144
Size: 1490 2649
XmaI C'CCGG_G
Cuts at: 236 1159 236
Size: 923 3216
XmnI GAAnn'nnTTC
Cuts at: 1006 1006
Size: 4139
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI