Restriction map for
pF1KB3738
(Circular) (Six-base) MAPSORT of: pF1KB3738.seq Check: 6162 from: 1 to: 4184
pF1KB3738 4184 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2009 2441 3188 3456 2009
Size: 432 747 268 2737
Fragments arranged by size:
2737 747 432 268
AflIII A'CryG_T
Cuts at: 1013 1536 2640 3617 1013
Size: 523 1104 977 1580
Fragments arranged by size:
1580 1104 977 523
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AhdI GACnn_n'nnGTC
Cuts at: 631 631
Size: 4184
AlwNI CAG_nnn'CTG
Cuts at: 1489 3056 1489
Size: 1567 2617
ApaLI G'TGCA_C
Cuts at: 148 855 2954 148
Size: 707 2099 1378
Fragments arranged by size:
2099 1378 707
ApoI r'AATT_y
Cuts at: 282 418 1188 3537 3793 282
Size: 136 770 2349 256 673
Fragments arranged by size:
2349 770 673 256 136
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 404 1204 404
Size: 800 3384
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 935 968 935
Size: 33 4151
BamHI G'GATC_C
Cuts at: 590 1209 590
Size: 619 3565
BanI G'GyrC_C
Cuts at: 562 1200 1614 1862 1897 562
Size: 638 414 248 35 2849
Fragments arranged by size:
2849 638 414 248 35
BanII G_rGCy'C
Cuts at: 793 937 1198 2228 793
Size: 144 261 1030 2749
Fragments arranged by size:
2749 1030 261 144
BbeI G_GCGC'C
Cuts at: 566 1618 1866 566
Size: 1052 248 2884
Fragments arranged by size:
2884 1052 248
BbvCI CC'TCA_GC
Cuts at: 217 217
Size: 4184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 379 413 1017 1051 379
Size: 34 604 34 3512
Fragments arranged by size:
3512 604 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2082 2843 4184 2082
Size: 761 1341 2082
Fragments arranged by size:
2082 1341 761
BclI T'GATC_A
Cuts at: 1704 1704
Size: 4184
BfrBI ATG'CAT
Cuts at: 225 225
Size: 4184
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4184
BglII A'GATC_T
Cuts at: 776 1699 3736 776
Size: 923 2037 1224
Fragments arranged by size:
2037 1224 923
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 152 859 1809 1902 2958 152
Size: 707 950 93 1056 1378
Fragments arranged by size:
1378 1056 950 707 93
BmrI ACTGGGnnnn_n'
Cuts at: 270 1157 1396 1477 1812 3956 270
Size: 887 239 81 335 2144 498
Fragments arranged by size:
2144 887 498 335 239 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 757 1111 1123 193
Size: 564 354 12 3254
Fragments arranged by size:
3254 564 354 12
Bpu10I CC'TnA_GC
Cuts at: 217 217
Size: 4184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 1135 1360 2239 2731 3029 686
Size: 449 225 879 492 298 1841
Fragments arranged by size:
1841 879 492 449 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 3888 3888
Size: 4184
BsaAI yAC'GTr
Cuts at: 1016 1537 1016
Size: 521 3663
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 563 1615 1863 2565 4061 563
Size: 1052 248 702 1496 686
Fragments arranged by size:
1496 1052 702 686 248
BsaWI w'CCGG_w
Cuts at: 1373 1894 2572 2846 2993 3404 1373
Size: 521 678 274 147 411 2153
Fragments arranged by size:
2153 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 309 649 825 930 1609 2367 2944 3440 309
Size: 340 176 105 679 758 577 496 1053
Fragments arranged by size:
1053 758 679 577 496 340 176 105
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 152 402 859 1198 1976 2166 2958 152
Size: 250 457 339 778 190 792 1378
Fragments arranged by size:
1378 792 778 457 339 250 190
BsmI GAATG_Cn'
Cuts at: 225 225
Size: 4184
Bsp1286I G_dGCh'C
Cuts at: 152 402 793 859 937 1198 1809 1902 1976
Size: 250 391 66 78 261 611 93 74
Cuts at: 1976 2166 2228 2958 152
Size: 190 62 730 1378
Fragments arranged by size:
1378 730 611 391 261 250 190 93
78 74 66 62
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 3360 3360
Size: 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1234 1448 1750 2131 1234
Size: 214 302 381 3287
Fragments arranged by size:
3287 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2476 2530 4014 4179 50
Size: 2426 54 1484 165 55
Fragments arranged by size:
2426 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 695 2096 695
Size: 1401 2783
BsrFI r'CCGG_y
Cuts at: 497 2182 2363 2572 497
Size: 1685 181 209 2109
Fragments arranged by size:
2109 1685 209 181
BssSI C'ACGA_G
Cuts at: 676 916 2455 2813 676
Size: 240 1539 358 2047
Fragments arranged by size:
2047 1539 358 240
BstAPI GCAn_nnn'nTGC
Cuts at: 1561 1561
Size: 4184
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 309 690 309
Size: 381 3803
BstYI r'GATC_y
Cuts at: 1 590 776 1209 1676 1699 2034 2280 3292
Size: 589 186 433 467 23 335 246 1012
Cuts at: 3292 3378 3390 3736 1
Size: 86 12 346 449
Fragments arranged by size:
1012 589 467 449 433 346 335 246
186 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 794 794
Size: 4184
BtgI C'CryG_G
Cuts at: 107 302 107
Size: 195 3989
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 1427 1427
Size: 4184
DraI TTT'AAA
Cuts at: 1122 1183 1122
Size: 61 4123
DrdI GACnn_nn'nnGTC
Cuts at: 720 1890 2748 720
Size: 1170 858 2156
Fragments arranged by size:
2156 1170 858
EaeI y'GGCC_r
Cuts at: 119 680 1018 1769 1943 2334 2361 3706 119
Size: 561 338 751 174 391 27 1345 597
Fragments arranged by size:
1345 751 597 561 391 338 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 626 2392 2702 2848 3994 626
Size: 1766 310 146 1146 816
Fragments arranged by size:
1766 1146 816 310 146
EcoICRI GAG'CTC
Cuts at: 1196 1196
Size: 4184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 757 1111 1123 2009 2441 3188 3456 193
Size: 564 354 12 886 432 747 268 921
Fragments arranged by size:
921 886 747 564 432 354 268 12
EcoNI CCTnn'n_nnAGG
Cuts at: 194 194
Size: 4184
EcoO109I rG'GnC_Cy
Cuts at: 633 911 1323 633
Size: 278 412 3494
Fragments arranged by size:
3494 412 278
EcoRI G'AATT_C
Cuts at: 418 1188 418
Size: 770 3414
EcoRV GAT'ATC
Cuts at: 987 987
Size: 4184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 4184
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 566 1618 1866 2888 3867 566
Size: 1052 248 1022 979 883
Fragments arranged by size:
1052 1022 979 883 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 617 649 2026 2056 2058 2088 617
Size: 32 1377 30 2 30 2713
Fragments arranged by size:
2713 1377 32 30 30 2
HincII GTy'rAC
Cuts at: 1223 1223
Size: 4184
HindIII A'AGCT_T
Cuts at: 320 532 320
Size: 212 3972
KasI G'GCGC_C
Cuts at: 562 1614 1862 562
Size: 1052 248 2884
Fragments arranged by size:
2884 1052 248
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 213 2855 3039 213
Size: 2642 184 1358
Fragments arranged by size:
2642 1358 184
MscI TGG'CCA
Cuts at: 682 1020 1945 682
Size: 338 925 2921
Fragments arranged by size:
2921 925 338
MslI CAynn'nnrTG
Cuts at: 2173 2300 2173
Size: 127 4057
MspA1I CmG'CkG
Cuts at: 825 1295 1609 1969 2982 3227 825
Size: 470 314 360 1013 245 1782
Fragments arranged by size:
1782 1013 470 360 314 245
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 563 1615 1863 563
Size: 1052 248 2884
Fragments arranged by size:
2884 1052 248
NcoI C'CATG_G
Cuts at: 107 302 107
Size: 195 3989
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NsiI A_TGCA'T
Cuts at: 227 227
Size: 4184
NspI r_CATG'y
Cuts at: 967 1237 1737 2644 967
Size: 270 500 907 2507
Fragments arranged by size:
2507 907 500 270
PasI CC'CwG_GG
Cuts at: 621 621
Size: 4184
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PfoI T'CCnGG_A
Cuts at: 974 974
Size: 4184
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PmlI CAC'GTG
Cuts at: 1016 1016
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4152
PpuMI rG'GwC_Cy
Cuts at: 633 911 633
Size: 278 3906
PstI C_TGCA'G
Cuts at: 604 1231 1916 604
Size: 627 685 2872
Fragments arranged by size:
2872 685 627
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 825 1609 1969 825
Size: 784 360 3040
Fragments arranged by size:
3040 784 360
RsrII CG'GwC_CG
Cuts at: 154 2379 154
Size: 2225 1959
SacI G_AGCT'C
Cuts at: 1198 1198
Size: 4184
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SanDI GG'GwC_CC
Cuts at: 911 911
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
SbfI CC_TGCA'GG
Cuts at: 604 1231 604
Size: 627 3557
ScaI AGT'ACT
Cuts at: 3701 3701
Size: 4184
SexAI A'CCwGG_T
Cuts at: 369 369
Size: 4184
SfcI C'TryA_G
Cuts at: 33 600 1227 1912 2905 3096 33
Size: 567 627 685 993 191 1121
Fragments arranged by size:
1121 993 685 627 567 191
SfoI GGC'GCC
Cuts at: 564 1616 1864 564
Size: 1052 248 2884
Fragments arranged by size:
2884 1052 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 701 1150 1339 2254 2746 3008 701
Size: 449 189 915 492 262 1877
Fragments arranged by size:
1877 915 492 449 262 189
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 967 1237 1737 967
Size: 270 500 3414
Fragments arranged by size:
3414 500 270
StuI AGG'CCT
Cuts at: 871 1108 871
Size: 237 3947
StyI C'CwwG_G
Cuts at: 107 302 746 782 1318 1672 107
Size: 195 444 36 536 354 2619
Fragments arranged by size:
2619 536 444 354 195 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1090 1767 1900 2359 2568 1090
Size: 677 133 459 209 2706
Fragments arranged by size:
2706 677 459 209 133
TatI w'GTAC_w
Cuts at: 3699 3699
Size: 4184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 4152
Tth111I GACn'n_nGTC
Cuts at: 544 1981 544
Size: 1437 2747
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 978 1679 978
Size: 701 3483
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BbsI BmgBI BmtI BsaXI BseRI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PflMI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI