Restriction map for
pF1KB3717
(Circular) (Six-base) MAPSORT of: pF1KB3717.seq Check: 6853 from: 1 to: 4547
pF1KB3717 4547 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 206 289 1044 1377 1585 206
Size: 83 755 333 208 3168
Fragments arranged by size:
3168 755 333 208 83
Acc65I G'GTAC_C
Cuts at: 1563 1563
Size: 4547
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2372 2804 3157 3819 1402
Size: 970 432 353 662 2130
Fragments arranged by size:
2130 970 662 432 353
AflIII A'CryG_T
Cuts at: 1899 3699 3980 1899
Size: 1800 281 2466
Fragments arranged by size:
2466 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1373 1373
Size: 4547
AlwNI CAG_nnn'CTG
Cuts at: 975 1091 1852 3290 975
Size: 116 761 1438 2232
Fragments arranged by size:
2232 1438 761 116
ApaLI G'TGCA_C
Cuts at: 553 1103 3385 553
Size: 550 2282 1715
Fragments arranged by size:
2282 1715 550
ApoI r'AATT_y
Cuts at: 1551 3900 4156 1551
Size: 2349 256 1942
Fragments arranged by size:
2349 1942 256
AseI AT'TA_AT
Cuts at: 20 851 1214 20
Size: 831 363 3353
Fragments arranged by size:
3353 831 363
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 185 350 1567 185
Size: 165 1217 3165
Fragments arranged by size:
3165 1217 165
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 198 231 198
Size: 33 4514
BamHI G'GATC_C
Cuts at: 1492 1572 1492
Size: 80 4467
BanI G'GyrC_C
Cuts at: 1563 1977 2225 2260 1563
Size: 414 248 35 3850
Fragments arranged by size:
3850 414 248 35
BanII G_rGCy'C
Cuts at: 1069 1561 2591 1069
Size: 492 1030 3025
Fragments arranged by size:
3025 1030 492
BbeI G_GCGC'C
Cuts at: 1981 2229 1981
Size: 248 4299
BbsI GAAGACnn'nnnn_
Cuts at: 183 770 903 1196 183
Size: 587 133 293 3534
Fragments arranged by size:
3534 587 293 133
BciVI GTATCCnnnnn_n'
Cuts at: 2445 3501 4547 2445
Size: 1056 1046 2445
Fragments arranged by size:
2445 1056 1046
BclI T'GATC_A
Cuts at: 1406 2067 1406
Size: 661 3886
BglII A'GATC_T
Cuts at: 2062 4099 2062
Size: 2037 2510
BlpI GC'TnA_GC
Cuts at: 1659 1659
Size: 4547
Bme1580I G_kGCm'C
Cuts at: 557 1107 2172 2265 3389 557
Size: 550 1065 93 1124 1715
Fragments arranged by size:
1715 1124 1065 550 93
BmrI ACTGGGnnnn_n'
Cuts at: 1321 1759 1840 2175 4319 1321
Size: 438 81 335 2144 1549
Fragments arranged by size:
2144 1549 438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1546 2914 1546
Size: 1368 3179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 779
Size: 4547
Bpu10I CC'TnA_GC
Cuts at: 1068 1068
Size: 4547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1723 2602 3316 3614 1723
Size: 879 714 298 2656
Fragments arranged by size:
2656 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 337 464 479 4251 337
Size: 127 15 3772 633
Fragments arranged by size:
3772 633 127 15
BsaAI yAC'GTr
Cuts at: 1900 1900
Size: 4547
BsaBI GATnn'nnATC
Cuts at: 6 2061 4098 6
Size: 2055 2037 455
Fragments arranged by size:
2055 2037 455
BsaHI Gr'CG_yC
Cuts at: 1978 2226 2928 4424 1978
Size: 248 702 1496 2101
Fragments arranged by size:
2101 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1736 2257 2935 3346 3493 3767 1736
Size: 521 678 411 147 274 2516
Fragments arranged by size:
2516 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 731 761 731
Size: 30 4517
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 340
Size: 4547
BseYI C'CCAG_C
Cuts at: 1440 1972 2730 3395 3803 1440
Size: 532 758 665 408 2184
Fragments arranged by size:
2184 758 665 532 408
BsiEI CG_ry'CG
Cuts at: 104 2089 2135 3365 104
Size: 1985 46 1230 1286
Fragments arranged by size:
1985 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 557 1107 1527 1561 2339 2529 3389 557
Size: 550 420 34 778 190 860 1715
Fragments arranged by size:
1715 860 778 550 420 190 34
BsmBI CGTCTCn'nnnn_
Cuts at: 1360 1360
Size: 4547
Bsp1286I G_dGCh'C
Cuts at: 557 1069 1107 1527 1561 2172 2265 2339 2529
Size: 512 38 420 34 611 93 74 190
Cuts at: 2529 2591 3389 557
Size: 62 798 1715
Fragments arranged by size:
1715 798 611 512 420 190 93 74
62 38 34
BspHI T'CATG_A
Cuts at: 2979 2979
Size: 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 920 1597 1811 2113 2494 920
Size: 677 214 302 381 2973
Fragments arranged by size:
2973 677 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2839 2893 4377 4542 50
Size: 2789 54 1484 165 55
Fragments arranged by size:
2789 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2459 2459
Size: 4547
BsrFI r'CCGG_y
Cuts at: 1033 2545 2726 1033
Size: 1512 181 2854
Fragments arranged by size:
2854 1512 181
BsrGI T'GTAC_A
Cuts at: 966 966
Size: 4547
BssSI C'ACGA_G
Cuts at: 2818 3526 2818
Size: 708 3839
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 1924
Size: 4547
BstBI TT'CG_AA
Cuts at: 1536 2908 1536
Size: 1372 3175
BstEII G'GTnAC_C
Cuts at: 159 159
Size: 4547
BstXI CCAn_nnnn'nTGG
Cuts at: 481 481
Size: 4547
BstYI r'GATC_y
Cuts at: 1 329 1492 1572 2039 2062 2397 2643 2949
Size: 328 1163 80 467 23 335 246 306
Cuts at: 2949 2961 3047 4099 1
Size: 12 86 1052 449
Fragments arranged by size:
1163 1052 467 449 335 328 306 246
86 80 23 12
BstZ17I GTA'TAC
Cuts at: 290 1045 290
Size: 755 3792
Bsu36I CC'TnA_GG
Cuts at: 562 1049 562
Size: 487 4060
BtgI C'CryG_G
Cuts at: 107 1316 1496 107
Size: 1209 180 3158
Fragments arranged by size:
3158 1209 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2497 2678 2841 2497
Size: 181 163 4203
Fragments arranged by size:
4203 181 163
BtsI GCAGTG_nn'
Cuts at: 1790 1790
Size: 4547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 773 808 773
Size: 35 4512
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 2253 3597 2253
Size: 1344 3203
EaeI y'GGCC_r
Cuts at: 2132 2306 2697 2724 4069 2132
Size: 174 391 27 1345 2610
Fragments arranged by size:
2610 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2132 2132
Size: 4547
EarI CTCTTCn'nnn_
Cuts at: 2570 2780 2570
Size: 210 4337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2755 3497 3643 4357 2755
Size: 742 146 714 2945
Fragments arranged by size:
2945 742 714 146
EcoICRI GAG'CTC
Cuts at: 1559 1559
Size: 4547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 1402 2372 2804 3157 3819 779
Size: 623 970 432 353 662 1507
Fragments arranged by size:
1507 970 662 623 432 353
EcoO109I rG'GnC_Cy
Cuts at: 900 1686 900
Size: 786 3761
EcoRI G'AATT_C
Cuts at: 1551 1551
Size: 4547
EcoRV GAT'ATC
Cuts at: 690 690
Size: 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4547
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 1441 1981 2229 3459 4230 1441
Size: 540 248 1230 771 1758
Fragments arranged by size:
1758 1230 771 540 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1359 1391 2389 2419 2421 2451 1359
Size: 32 998 30 2 30 3455
Fragments arranged by size:
3455 998 32 30 30 2
HincII GTy'rAC
Cuts at: 627 1378 1586 627
Size: 751 208 3588
Fragments arranged by size:
3588 751 208
KasI G'GCGC_C
Cuts at: 1977 2225 1977
Size: 248 4299
KpnI G_GTAC'C
Cuts at: 1567 1567
Size: 4547
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 462 3306 3490 462
Size: 2844 184 1519
Fragments arranged by size:
2844 1519 184
MscI TGG'CCA
Cuts at: 2308 2308
Size: 4547
MslI CAynn'nnrTG
Cuts at: 1114 1432 2536 2663 1114
Size: 318 1104 127 2998
Fragments arranged by size:
2998 1104 318 127
MspA1I CmG'CkG
Cuts at: 1513 1658 1972 2332 3116 3361 1513
Size: 145 314 360 784 245 2699
Fragments arranged by size:
2699 784 360 314 245 145
NaeI GCC'GGC
Cuts at: 1035 2728 1035
Size: 1693 2854
NarI GG'CG_CC
Cuts at: 1978 2226 1978
Size: 248 4299
NcoI C'CATG_G
Cuts at: 107 1316 1496 107
Size: 1209 180 3158
Fragments arranged by size:
3158 1209 180
NgoMIV G'CCGG_C
Cuts at: 1033 2726 1033
Size: 1693 2854
NspI r_CATG'y
Cuts at: 1176 1481 1600 2100 3703 1176
Size: 305 119 500 1603 2020
Fragments arranged by size:
2020 1603 500 305 119
PciI A'CATG_T
Cuts at: 3699 3699
Size: 4547
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 3025 2993
Size: 32 4515
PpuMI rG'GwC_Cy
Cuts at: 900 900
Size: 4547
PshAI GACnn'nnGTC
Cuts at: 166 1383 166
Size: 1217 3330
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 280 312 280
Size: 32 4515
PstI C_TGCA'G
Cuts at: 1005 1594 2279 1005
Size: 589 685 3273
Fragments arranged by size:
3273 685 589
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 1972 2332 1972
Size: 360 4187
RsrII CG'GwC_CG
Cuts at: 2742 2742
Size: 4547
SacI G_AGCT'C
Cuts at: 1561 1561
Size: 4547
SalI G'TCGA_C
Cuts at: 1376 1584 1376
Size: 208 4339
SapI GCTCTTCn'nnn_
Cuts at: 2570 2780 2570
Size: 210 4337
SbfI CC_TGCA'GG
Cuts at: 1594 1594
Size: 4547
ScaI AGT'ACT
Cuts at: 4064 4064
Size: 4547
SfcI C'TryA_G
Cuts at: 33 203 378 1001 1590 2275 3243 3434 33
Size: 170 175 623 589 685 968 191 1146
Fragments arranged by size:
1146 968 685 623 589 191 175 170
SfoI GGC'GCC
Cuts at: 1979 2227 1979
Size: 248 4299
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SmaI CCC'GGG
Cuts at: 187 1569 187
Size: 1382 3165
SmlI C'TyrA_G
Cuts at: 1702 2617 3331 3593 1702
Size: 915 714 262 2656
Fragments arranged by size:
2656 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 1176 1481 1600 2100 1176
Size: 305 119 500 3623
Fragments arranged by size:
3623 500 305 119
SspI AAT'ATT
Cuts at: 405 519 1010 405
Size: 114 491 3942
Fragments arranged by size:
3942 491 114
StuI AGG'CCT
Cuts at: 454 454
Size: 4547
StyI C'CwwG_G
Cuts at: 107 1316 1496 1681 2035 107
Size: 1209 180 185 354 2619
Fragments arranged by size:
2619 1209 354 185 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 893 1122 1193 1432 2130 2263 2722 2931 893
Size: 229 71 239 698 133 459 209 2509
Fragments arranged by size:
2509 698 459 239 229 209 133 71
TatI w'GTAC_w
Cuts at: 966 4062 966
Size: 3096 1451
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 4515
Tth111I GACn'n_nGTC
Cuts at: 141 2344 141
Size: 2203 2344
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2042 2042
Size: 4547
XmaI C'CCGG_G
Cuts at: 185 1567 185
Size: 1382 3165
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BspEI BssHII ClaI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI