Restriction map for
pF1KB3715
(Circular) (Six-base) MAPSORT of: pF1KB3715.seq Check: 4066 from: 1 to: 3737
pF1KB3715 3737 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 190 440 190
Size: 250 3487
AccI GT'mk_AC
Cuts at: 775 775
Size: 3737
Acc65I G'GTAC_C
Cuts at: 753 753
Size: 3737
AclI AA'CG_TT
Cuts at: 3577 3577
Size: 3737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1994 2741 3009 1562
Size: 432 747 268 2290
Fragments arranged by size:
2290 747 432 268
AflIII A'CryG_T
Cuts at: 612 1089 2193 3170 612
Size: 477 1104 977 1179
Fragments arranged by size:
1179 1104 977 477
AgeI A'CCGG_T
Cuts at: 2125 2125
Size: 3737
AhdI GACnn_n'nnGTC
Cuts at: 258 258
Size: 3737
AlwNI CAG_nnn'CTG
Cuts at: 1042 2609 1042
Size: 1567 2170
ApaLI G'TGCA_C
Cuts at: 2507 2507
Size: 3737
ApoI r'AATT_y
Cuts at: 145 741 3090 3346 145
Size: 596 2349 256 536
Fragments arranged by size:
2349 596 536 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3737
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
AvaI C'yCGr_G
Cuts at: 757 757
Size: 3737
BamHI G'GATC_C
Cuts at: 762 762
Size: 3737
BanI G'GyrC_C
Cuts at: 753 1167 1415 1450 753
Size: 414 248 35 3040
Fragments arranged by size:
3040 414 248 35
BanII G_rGCy'C
Cuts at: 704 751 1781 704
Size: 47 1030 2660
Fragments arranged by size:
2660 1030 47
BbeI G_GCGC'C
Cuts at: 1171 1419 1171
Size: 248 3489
BbsI GAAGACnn'nnnn_
Cuts at: 190 586 190
Size: 396 3341
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 589 623 589
Size: 34 3703
BciVI GTATCCnnnnn_n'
Cuts at: 1635 2396 3737 1635
Size: 761 1341 1635
Fragments arranged by size:
1635 1341 761
BclI T'GATC_A
Cuts at: 1257 1257
Size: 3737
BglII A'GATC_T
Cuts at: 1252 3289 1252
Size: 2037 1700
BlpI GC'TnA_GC
Cuts at: 849 849
Size: 3737
Bme1580I G_kGCm'C
Cuts at: 1362 1455 2511 1362
Size: 93 1056 2588
Fragments arranged by size:
2588 1056 93
BmgBI CAC'GTC
Cuts at: 613 613
Size: 3737
BmrI ACTGGGnnnn_n'
Cuts at: 949 1030 1365 3509 949
Size: 81 335 2144 1177
Fragments arranged by size:
2144 1177 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 3737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 1792 2284 2582 913
Size: 879 492 298 2068
Fragments arranged by size:
2068 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 562 3441 562
Size: 2879 858
BsaAI yAC'GTr
Cuts at: 1090 1090
Size: 3737
BsaBI GATnn'nnATC
Cuts at: 6 517 676 1251 3288 6
Size: 511 159 575 2037 455
Fragments arranged by size:
2037 575 511 455 159
BsaHI Gr'CG_yC
Cuts at: 187 437 619 1168 1416 2118 3614 187
Size: 250 182 549 248 702 1496 310
Fragments arranged by size:
1496 702 549 310 250 248 182
BsaWI w'CCGG_w
Cuts at: 926 1447 2125 2399 2546 2957 926
Size: 521 678 274 147 411 1706
Fragments arranged by size:
1706 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 538
Size: 3737
BseYI C'CCAG_C
Cuts at: 346 1162 1920 2497 2993 346
Size: 816 758 577 496 1090
Fragments arranged by size:
1090 816 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1279 1325 2533 104
Size: 1175 46 1208 1308
Fragments arranged by size:
1308 1208 1175 46
BsiHKAI G_wGCw'C
Cuts at: 751 1529 1719 2511 751
Size: 778 190 792 1977
Fragments arranged by size:
1977 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 194 194
Size: 3737
Bsp1286I G_dGCh'C
Cuts at: 704 751 1362 1455 1529 1719 1781 2511 704
Size: 47 611 93 74 190 62 730 1930
Fragments arranged by size:
1930 730 611 190 93 74 62 47
BspEI T'CCGG_A
Cuts at: 2957 2957
Size: 3737
BspHI T'CATG_A
Cuts at: 2913 2913
Size: 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 787 1001 1303 1684 787
Size: 214 302 381 2840
Fragments arranged by size:
2840 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2029 2083 3567 3732 50
Size: 1979 54 1484 165 55
Fragments arranged by size:
1979 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1649 1649
Size: 3737
BsrFI r'CCGG_y
Cuts at: 1735 1916 2125 1735
Size: 181 209 3347
Fragments arranged by size:
3347 209 181
BssHII G'CGCG_C
Cuts at: 537 537
Size: 3737
BssSI C'ACGA_G
Cuts at: 2008 2366 2008
Size: 358 3379
BstAPI GCAn_nnn'nTGC
Cuts at: 1114 1114
Size: 3737
BstBI TT'CG_AA
Cuts at: 2098 2098
Size: 3737
BstXI CCAn_nnnn'nTGG
Cuts at: 276 276
Size: 3737
BstYI r'GATC_y
Cuts at: 1 762 1229 1252 1587 1833 2845 2931 2943
Size: 761 467 23 335 246 1012 86 12
Cuts at: 2943 3289 1
Size: 346 449
Fragments arranged by size:
1012 761 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 365 365
Size: 3737
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1687 1868 2031 1687
Size: 181 163 3393
Fragments arranged by size:
3393 181 163
BtsI GCAGTG_nn'
Cuts at: 462 980 462
Size: 518 3219
DraI TTT'AAA
Cuts at: 736 736
Size: 3737
DrdI GACnn_nn'nnGTC
Cuts at: 1443 2301 1443
Size: 858 2879
EaeI y'GGCC_r
Cuts at: 707 1322 1496 1887 1914 3259 707
Size: 615 174 391 27 1345 1185
Fragments arranged by size:
1345 1185 615 391 174 27
EagI C'GGCC_G
Cuts at: 1322 1322
Size: 3737
EarI CTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1945 2255 2401 3547 1945
Size: 310 146 1146 2135
Fragments arranged by size:
2135 1146 310 146
EcoICRI GAG'CTC
Cuts at: 749 749
Size: 3737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1994 2741 3009 1562
Size: 432 747 268 2290
Fragments arranged by size:
2290 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 876 876
Size: 3737
EcoRI G'AATT_C
Cuts at: 741 741
Size: 3737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1756 1756
Size: 3737
FspI TGC'GCA
Cuts at: 1518 1518
Size: 3737
HaeII r_GCGC'y
Cuts at: 1171 1419 2441 3420 1171
Size: 248 1022 979 1488
Fragments arranged by size:
1488 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 287 319 1579 1609 1611 1641 244
Size: 32 11 32 1260 30 2 30 2340
Fragments arranged by size:
2340 1260 32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 776 776
Size: 3737
KasI G'GCGC_C
Cuts at: 1167 1415 1167
Size: 248 3489
KpnI G_GTAC'C
Cuts at: 757 757
Size: 3737
MluI A'CGCG_T
Cuts at: 3170 3170
Size: 3737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 435 2408 2592 435
Size: 1973 184 1580
Fragments arranged by size:
1973 1580 184
MscI TGG'CCA
Cuts at: 1498 1498
Size: 3737
MslI CAynn'nnrTG
Cuts at: 475 1726 1853 475
Size: 1251 127 2359
Fragments arranged by size:
2359 1251 127
MspA1I CmG'CkG
Cuts at: 316 848 1162 1522 2535 2780 316
Size: 532 314 360 1013 245 1273
Fragments arranged by size:
1273 1013 532 360 314 245
NaeI GCC'GGC
Cuts at: 1918 1918
Size: 3737
NarI GG'CG_CC
Cuts at: 1168 1416 1168
Size: 248 3489
NgoMIV G'CCGG_C
Cuts at: 1916 1916
Size: 3737
NspI r_CATG'y
Cuts at: 790 1290 2197 790
Size: 500 907 2330
Fragments arranged by size:
2330 907 500
PciI A'CATG_T
Cuts at: 2193 2193
Size: 3737
PfoI T'CCnGG_A
Cuts at: 356 647 356
Size: 291 3446
PmeI GTTT'AAAC
Cuts at: 736 736
Size: 3737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2876 2908 2876
Size: 32 3705
PstI C_TGCA'G
Cuts at: 302 784 1469 302
Size: 482 685 2570
Fragments arranged by size:
2570 685 482
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3737
PvuII CAG'CTG
Cuts at: 316 1162 1522 316
Size: 846 360 2531
Fragments arranged by size:
2531 846 360
RsrII CG'GwC_CG
Cuts at: 1932 1932
Size: 3737
SacI G_AGCT'C
Cuts at: 751 751
Size: 3737
SalI G'TCGA_C
Cuts at: 774 774
Size: 3737
SapI GCTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
SbfI CC_TGCA'GG
Cuts at: 784 784
Size: 3737
ScaI AGT'ACT
Cuts at: 3254 3254
Size: 3737
SfcI C'TryA_G
Cuts at: 33 298 780 1465 2458 2649 33
Size: 265 482 685 993 191 1121
Fragments arranged by size:
1121 993 685 482 265 191
SfoI GGC'GCC
Cuts at: 1169 1417 1169
Size: 248 3489
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
SmaI CCC'GGG
Cuts at: 759 759
Size: 3737
SmlI C'TyrA_G
Cuts at: 892 1807 2299 2561 892
Size: 915 492 262 2068
Fragments arranged by size:
2068 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3737
SphI G_CATG'C
Cuts at: 790 1290 790
Size: 500 3237
StyI C'CwwG_G
Cuts at: 871 1225 871
Size: 354 3383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1320 1453 1912 2121 1320
Size: 133 459 209 2936
Fragments arranged by size:
2936 459 209 133
TatI w'GTAC_w
Cuts at: 272 3252 272
Size: 2980 757
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 3705
Tth111I GACn'n_nGTC
Cuts at: 436 1534 436
Size: 1098 2639
XbaI T'CTAG_A
Cuts at: 768 768
Size: 3737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 241 372 1232 241
Size: 131 860 2746
Fragments arranged by size:
2746 860 131
XmaI C'CCGG_G
Cuts at: 757 757
Size: 3737
ZraI GAC'GTC
Cuts at: 188 438 188
Size: 250 3487
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI BpmI Bpu10I
BsaXI BsgI BsiWI BsmI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI