Restriction map for
pF1KB3705
(Circular) (Six-base) MAPSORT of: pF1KB3705.seq Check: 4 from: 1 to: 6323
pF1KB3705 6323 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 2474 3361 2474
Size: 887 5436
Acc65I G'GTAC_C
Cuts at: 243 3339 243
Size: 3096 3227
AclI AA'CG_TT
Cuts at: 798 6163 798
Size: 5365 958
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 557 1864 1876 4148 4580 4933 5595 99
Size: 458 1307 12 2272 432 353 662 827
Fragments arranged by size:
2272 1307 827 662 458 432 353 12
AflII C'TTAA_G
Cuts at: 3178 3178
Size: 6323
AflIII A'CryG_T
Cuts at: 731 3675 5475 5756 731
Size: 2944 1800 281 1298
Fragments arranged by size:
2944 1800 1298 281
AhdI GACnn_n'nnGTC
Cuts at: 2382 2382
Size: 6323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2177 2177
Size: 6323
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 641 673 641
Size: 32 6291
AlwNI CAG_nnn'CTG
Cuts at: 1887 3628 5066 1887
Size: 1741 1438 3144
Fragments arranged by size:
3144 1741 1438
ApaLI G'TGCA_C
Cuts at: 2152 5161 2152
Size: 3009 3314
ApoI r'AATT_y
Cuts at: 514 547 568 1026 1264 1588 2806 2937 3238
Size: 33 21 458 238 324 1218 131 301
Cuts at: 3238 3327 5676 5932 514
Size: 89 2349 256 905
Fragments arranged by size:
2349 1218 905 458 324 301 256 238
131 89 33 21
AseI AT'TA_AT
Cuts at: 20 1274 1367 20
Size: 1254 93 4976
Fragments arranged by size:
4976 1254 93
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6323
AvaI C'yCGr_G
Cuts at: 248 1916 3343 248
Size: 1668 1427 3228
Fragments arranged by size:
3228 1668 1427
BamHI G'GATC_C
Cuts at: 531 1740 3348 531
Size: 1209 1608 3506
Fragments arranged by size:
3506 1608 1209
BanI G'GyrC_C
Cuts at: 243 3339 3753 4001 4036 243
Size: 3096 414 248 35 2530
Fragments arranged by size:
3096 2530 414 248 35
BanII G_rGCy'C
Cuts at: 939 1196 3337 4367 939
Size: 257 2141 1030 2895
Fragments arranged by size:
2895 2141 1030 257
BbeI G_GCGC'C
Cuts at: 3757 4005 3757
Size: 248 6075
BbsI GAAGACnn'nnnn_
Cuts at: 405 1487 405
Size: 1082 5241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1781 1815 1781
Size: 34 6289
BciVI GTATCCnnnnn_n'
Cuts at: 4221 5277 6323 4221
Size: 1056 1046 4221
Fragments arranged by size:
4221 1056 1046
BclI T'GATC_A
Cuts at: 885 1239 3843 885
Size: 354 2604 3365
Fragments arranged by size:
3365 2604 354
BfrBI ATG'CAT
Cuts at: 2334 3073 2334
Size: 739 5584
BglI GCCn_nnn'nGGC
Cuts at: 1009 2466 1009
Size: 1457 4866
BglII A'GATC_T
Cuts at: 3313 3838 5875 3313
Size: 525 2037 3761
Fragments arranged by size:
3761 2037 525
BlpI GC'TnA_GC
Cuts at: 1874 3435 1874
Size: 1561 4762
Bme1580I G_kGCm'C
Cuts at: 2073 2156 2222 3948 4041 5165 2073
Size: 83 66 1726 93 1124 3231
Fragments arranged by size:
3231 1726 1124 93 83 66
BmgBI CAC'GTC
Cuts at: 732 732
Size: 6323
BmrI ACTGGGnnnn_n'
Cuts at: 230 3535 3616 3951 6095 230
Size: 3305 81 335 2144 458
Fragments arranged by size:
3305 2144 458 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4690 4690
Size: 6323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1490 1490
Size: 6323
Bpu10I CC'TnA_GC
Cuts at: 3253 3253
Size: 6323
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1882 3499 4378 5092 5390 1882
Size: 1617 879 714 298 2815
Fragments arranged by size:
2815 1617 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 6027 6027
Size: 6323
BsaAI yAC'GTr
Cuts at: 3676 3676
Size: 6323
BsaBI GATnn'nnATC
Cuts at: 6 229 3074 3837 5874 6
Size: 223 2845 763 2037 455
Fragments arranged by size:
2845 2037 763 455 223
BsaHI Gr'CG_yC
Cuts at: 3754 4002 4704 6200 3754
Size: 248 702 1496 3877
Fragments arranged by size:
3877 1496 702 248
BsaWI w'CCGG_w
Cuts at: 914 3512 4033 4711 5122 5269 5543 914
Size: 2598 521 678 411 147 274 1694
Fragments arranged by size:
2598 1694 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 391
Size: 6323
BseYI C'CCAG_C
Cuts at: 3748 4506 5171 5579 3748
Size: 758 665 408 4492
Fragments arranged by size:
4492 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3865 3911 5141 104
Size: 3761 46 1230 1286
Fragments arranged by size:
3761 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1196 2100 2156 2674 3337 4115 4305 5165 1196
Size: 904 56 518 663 778 190 860 2354
Fragments arranged by size:
2354 904 860 778 663 518 190 56
BsmI GAATG_Cn'
Cuts at: 967 2298 967
Size: 1331 4992
Bsp1286I G_dGCh'C
Cuts at: 939 1196 2073 2100 2156 2222 2674 3337 3948
Size: 257 877 27 56 66 452 663 611
Cuts at: 3948 4041 4115 4305 4367 5165 939
Size: 93 74 190 62 798 2097
Fragments arranged by size:
2097 877 798 663 611 452 257 190
93 74 66 62 56 27
BspEI T'CCGG_A
Cuts at: 914 914
Size: 6323
BspHI T'CATG_A
Cuts at: 4755 4755
Size: 6323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2232 2387 3373 3587 3889 4270 2232
Size: 155 986 214 302 381 4285
Fragments arranged by size:
4285 986 381 302 214 155
BsrBI CCG'CTC
Cuts at: 50 4615 4669 6153 6318 50
Size: 4565 54 1484 165 55
Fragments arranged by size:
4565 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2718 4235 2718
Size: 1517 4806
BsrFI r'CCGG_y
Cuts at: 3047 4321 4502 3047
Size: 1274 181 4868
Fragments arranged by size:
4868 1274 181
BssSI C'ACGA_G
Cuts at: 4594 5302 4594
Size: 708 5615
BstAPI GCAn_nnn'nTGC
Cuts at: 3700 3700
Size: 6323
BstBI TT'CG_AA
Cuts at: 552 4684 552
Size: 4132 2191
BstXI CCAn_nnnn'nTGG
Cuts at: 201 201
Size: 6323
BstYI r'GATC_y
Cuts at: 1 531 1740 1773 2604 3313 3348 3815 3838
Size: 530 1209 33 831 709 35 467 23
Cuts at: 3838 4173 4419 4725 4737 4823 5875 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1209 1052 831 709 530 467 449 335
306 246 86 35 33 23 12
BstZ17I GTA'TAC
Cuts at: 2475 2475
Size: 6323
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4273 4454 4617 4273
Size: 181 163 5979
Fragments arranged by size:
5979 181 163
BtsI GCAGTG_nn'
Cuts at: 1674 1822 3566 1674
Size: 148 1744 4431
Fragments arranged by size:
4431 1744 148
DraI TTT'AAA
Cuts at: 1452 2273 2345 2520 3322 1452
Size: 821 72 175 802 4453
Fragments arranged by size:
4453 821 802 175 72
DrdI GACnn_nn'nnGTC
Cuts at: 4029 5373 4029
Size: 1344 4979
EaeI y'GGCC_r
Cuts at: 2898 3045 3908 4082 4473 4500 5845 2898
Size: 147 863 174 391 27 1345 3376
Fragments arranged by size:
3376 1345 863 391 174 147 27
EagI C'GGCC_G
Cuts at: 3908 3908
Size: 6323
EarI CTCTTCn'nnn_
Cuts at: 1049 2911 3109 3196 4346 4556 1049
Size: 1862 198 87 1150 210 2816
Fragments arranged by size:
2816 1862 1150 210 198 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3043 4531 5273 5419 6133 3043
Size: 1488 742 146 714 3233
Fragments arranged by size:
3233 1488 742 714 146
EcoICRI GAG'CTC
Cuts at: 1194 3335 1194
Size: 2141 4182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 557 1490 1864 1876 4148 4580 4933 5595
Size: 458 933 374 12 2272 432 353 662
Cuts at: 5595 99
Size: 827
Fragments arranged by size:
2272 933 827 662 458 432 374 353
12
EcoO109I rG'GnC_Cy
Cuts at: 1923 3462 1923
Size: 1539 4784
EcoRI G'AATT_C
Cuts at: 3327 3327
Size: 6323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 697 2764 4342 697
Size: 2067 1578 2678
Fragments arranged by size:
2678 2067 1578
FspI TGC'GCA
Cuts at: 565 4104 565
Size: 3539 2784
HaeII r_GCGC'y
Cuts at: 1888 3253 3757 4005 5235 6006 1888
Size: 1365 504 248 1230 771 2205
Fragments arranged by size:
2205 1365 1230 771 504 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 319 351 1732 1764 2368 2400 3298 3330 4165
Size: 32 1381 32 604 32 898 32 835
Cuts at: 4165 4195 4197 4227 319
Size: 30 2 30 2415
Fragments arranged by size:
2415 1381 898 835 604 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2552 3131 3362 2552
Size: 579 231 5513
Fragments arranged by size:
5513 579 231
KasI G'GCGC_C
Cuts at: 3753 4001 3753
Size: 248 6075
KpnI G_GTAC'C
Cuts at: 247 3343 247
Size: 3096 3227
MfeI C'AATT_G
Cuts at: 1459 2684 1459
Size: 1225 5098
MluI A'CGCG_T
Cuts at: 5756 5756
Size: 6323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 218 2487 5082 5266 218
Size: 2269 2595 184 1275
Fragments arranged by size:
2595 2269 1275 184
MscI TGG'CCA
Cuts at: 2900 4084 2900
Size: 1184 5139
MslI CAynn'nnrTG
Cuts at: 199 2227 4312 4439 199
Size: 2028 2085 127 2083
Fragments arranged by size:
2085 2083 2028 127
MspA1I CmG'CkG
Cuts at: 860 1490 1654 2700 3434 3748 4108 4892 5137
Size: 630 164 1046 734 314 360 784 245
Cuts at: 5137 860
Size: 2046
Fragments arranged by size:
2046 1046 784 734 630 360 314 245
164
NaeI GCC'GGC
Cuts at: 4504 4504
Size: 6323
NarI GG'CG_CC
Cuts at: 3754 4002 3754
Size: 248 6075
NgoMIV G'CCGG_C
Cuts at: 4502 4502
Size: 6323
NsiI A_TGCA'T
Cuts at: 2336 3075 2336
Size: 739 5584
NspI r_CATG'y
Cuts at: 353 1585 3376 3876 5479 353
Size: 1232 1791 500 1603 1197
Fragments arranged by size:
1791 1603 1232 1197 500
PasI CC'CwG_GG
Cuts at: 460 460
Size: 6323
PciI A'CATG_T
Cuts at: 5475 5475
Size: 6323
PmeI GTTT'AAAC
Cuts at: 3322 3322
Size: 6323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4769 4801 4769
Size: 32 6291
PstI C_TGCA'G
Cuts at: 2384 2727 3138 3370 4055 2384
Size: 343 411 232 685 4652
Fragments arranged by size:
4652 685 411 343 232
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6323
PvuII CAG'CTG
Cuts at: 1490 1654 2700 3748 4108 1490
Size: 164 1046 1048 360 3705
Fragments arranged by size:
3705 1048 1046 360 164
RsrII CG'GwC_CG
Cuts at: 4518 4518
Size: 6323
SacI G_AGCT'C
Cuts at: 1196 3337 1196
Size: 2141 4182
SalI G'TCGA_C
Cuts at: 3360 3360
Size: 6323
SapI GCTCTTCn'nnn_
Cuts at: 2911 4346 4556 2911
Size: 1435 210 4678
Fragments arranged by size:
4678 1435 210
SbfI CC_TGCA'GG
Cuts at: 3370 3370
Size: 6323
ScaI AGT'ACT
Cuts at: 5840 5840
Size: 6323
SfcI C'TryA_G
Cuts at: 33 1418 1976 2380 2723 3134 3309 3366 4051
Size: 1385 558 404 343 411 175 57 685
Cuts at: 4051 5019 5210 33
Size: 968 191 1146
Fragments arranged by size:
1385 1146 968 685 558 411 404 343
191 175 57
SfoI GGC'GCC
Cuts at: 3755 4003 3755
Size: 248 6075
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6323
SmaI CCC'GGG
Cuts at: 1918 3345 1918
Size: 1427 4896
SmlI C'TyrA_G
Cuts at: 1861 3178 3478 4393 5107 5369 1861
Size: 1317 300 915 714 262 2815
Fragments arranged by size:
2815 1317 915 714 300 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6323
SphI G_CATG'C
Cuts at: 3376 3876 3376
Size: 500 5823
SspI AAT'ATT
Cuts at: 1278 1481 1278
Size: 203 6120
StyI C'CwwG_G
Cuts at: 2850 3457 3811 2850
Size: 607 354 5362
Fragments arranged by size:
5362 607 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2807 3906 4039 4498 4707 2807
Size: 1099 133 459 209 4423
Fragments arranged by size:
4423 1099 459 209 133
TatI w'GTAC_w
Cuts at: 2172 5838 2172
Size: 3666 2657
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5840 5872 5840
Size: 32 6291
Tth111I GACn'n_nGTC
Cuts at: 4120 4120
Size: 6323
XbaI T'CTAG_A
Cuts at: 2077 3354 2077
Size: 1277 5046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2917 3818 2917
Size: 901 5422
XmaI C'CCGG_G
Cuts at: 1916 3343 1916
Size: 1427 4896
XmnI GAAnn'nnTTC
Cuts at: 1074 3103 1074
Size: 2029 4294
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI ApaI AscI AvrII
BaeI BbvCI BmtI BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstEII Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI