Restriction map for
pF1KB3699
(Circular) (Six-base) MAPSORT of: pF1KB3699.seq Check: 1328 from: 1 to: 5666
pF1KB3699 5666 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 2704 2704
Size: 5666
Acc65I G'GTAC_C
Cuts at: 2682 2682
Size: 5666
AclI AA'CG_TT
Cuts at: 1332 5506 1332
Size: 4174 1492
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 964 1453 1493 1528 1831 1896 1897 2350
Size: 9 489 40 35 303 65 1 453
Cuts at: 2350 2508 3491 3923 4276 4938 955
Size: 158 983 432 353 662 1683
Fragments arranged by size:
1683 983 662 489 453 432 353 303
158 65 40 35 9 1
AflII C'TTAA_G
Cuts at: 2242 2242
Size: 5666
AflIII A'CryG_T
Cuts at: 3018 4818 5099 3018
Size: 1800 281 3585
Fragments arranged by size:
3585 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 342 342
Size: 5666
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 263 263
Size: 5666
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 488 520 874 906 488
Size: 32 354 32 5248
Fragments arranged by size:
5248 354 32 32
AlwNI CAG_nnn'CTG
Cuts at: 933 1038 1431 1443 2531 2971 4409 933
Size: 105 393 12 1088 440 1438 2190
Fragments arranged by size:
2190 1438 1088 440 393 105 12
ApaI G_GGCC'C
Cuts at: 483 1600 483
Size: 1117 4549
ApaLI G'TGCA_C
Cuts at: 4504 4504
Size: 5666
ApoI r'AATT_y
Cuts at: 373 458 1784 2670 5019 5275 373
Size: 85 1326 886 2349 256 764
Fragments arranged by size:
2349 1326 886 764 256 85
AseI AT'TA_AT
Cuts at: 20 20
Size: 5666
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5666
AvaI C'yCGr_G
Cuts at: 1270 2114 2686 1270
Size: 844 572 4250
Fragments arranged by size:
4250 844 572
BamHI G'GATC_C
Cuts at: 405 551 1704 2691 405
Size: 146 1153 987 3380
Fragments arranged by size:
3380 1153 987 146
BanI G'GyrC_C
Cuts at: 1894 2289 2682 3096 3344 3379 1894
Size: 395 393 414 248 35 4181
Fragments arranged by size:
4181 414 395 393 248 35
BanII G_rGCy'C
Cuts at: 483 855 1600 1747 2306 2402 2680 3710 483
Size: 372 745 147 559 96 278 1030 2439
Fragments arranged by size:
2439 1030 745 559 372 278 147 96
BbeI G_GCGC'C
Cuts at: 3100 3348 3100
Size: 248 5418
BbsI GAAGACnn'nnnn_
Cuts at: 233 615 664 1888 233
Size: 382 49 1224 4011
Fragments arranged by size:
4011 1224 382 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1992 2026 1992
Size: 34 5632
BciVI GTATCCnnnnn_n'
Cuts at: 3564 4620 5666 3564
Size: 1056 1046 3564
Fragments arranged by size:
3564 1056 1046
BclI T'GATC_A
Cuts at: 3186 3186
Size: 5666
BfrBI ATG'CAT
Cuts at: 2411 2411
Size: 5666
BglI GCCn_nnn'nGGC
Cuts at: 1751 1751
Size: 5666
BglII A'GATC_T
Cuts at: 3181 5218 3181
Size: 2037 3629
BlpI GC'TnA_GC
Cuts at: 1531 1657 2305 2778 1531
Size: 126 648 473 4419
Fragments arranged by size:
4419 648 473 126
Bme1580I G_kGCm'C
Cuts at: 483 1600 3291 3384 4508 483
Size: 1117 1691 93 1124 1641
Fragments arranged by size:
1691 1641 1124 1117 93
BmrI ACTGGGnnnn_n'
Cuts at: 408 2878 2959 3294 5438 408
Size: 2470 81 335 2144 636
Fragments arranged by size:
2470 2144 636 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4033 4033
Size: 5666
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 920 1141 1605 1810 2143 2257 2281 2392
Size: 19 221 464 205 333 114 24 111
Cuts at: 2392 2404 901
Size: 12 4163
Fragments arranged by size:
4163 464 333 221 205 114 111 24
19 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 2842 3721 4435 4733 1093
Size: 1749 879 714 298 2026
Fragments arranged by size:
2026 1749 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2205 5370 2205
Size: 3165 2501
BsaAI yAC'GTr
Cuts at: 3019 3019
Size: 5666
BsaBI GATnn'nnATC
Cuts at: 6 3180 5217 6
Size: 3174 2037 455
Fragments arranged by size:
3174 2037 455
BsaHI Gr'CG_yC
Cuts at: 3097 3345 4047 5543 3097
Size: 248 702 1496 3220
Fragments arranged by size:
3220 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1226 2855 3376 4054 4465 4612 4886 1226
Size: 1629 521 678 411 147 274 2006
Fragments arranged by size:
2006 1629 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 488 518 874 904 488
Size: 30 356 30 5250
Fragments arranged by size:
5250 356 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 829 1199 1611 2080 829
Size: 370 412 469 4415
Fragments arranged by size:
4415 469 412 370
BseYI C'CCAG_C
Cuts at: 827 1067 1593 2617 3091 3849 4514 4922 827
Size: 240 526 1024 474 758 665 408 1571
Fragments arranged by size:
1571 1024 758 665 526 474 408 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 233 1375 145
Size: 88 1142 4436
Fragments arranged by size:
4436 1142 88
BsiEI CG_ry'CG
Cuts at: 104 3208 3254 4484 104
Size: 3104 46 1230 1286
Fragments arranged by size:
3104 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 855 1958 1979 2306 2680 3458 3648 4508 855
Size: 1103 21 327 374 778 190 860 2013
Fragments arranged by size:
2013 1103 860 778 374 327 190 21
BsmBI CGTCTCn'nnnn_
Cuts at: 2613 2613
Size: 5666
Bsp1286I G_dGCh'C
Cuts at: 483 855 1600 1747 1958 1979 2306 2402 2680
Size: 372 745 147 211 21 327 96 278
Cuts at: 2680 3291 3384 3458 3648 3710 4508 483
Size: 611 93 74 190 62 798 1641
Fragments arranged by size:
1641 798 745 611 372 327 278 211
190 147 96 93 74 62 21
BspEI T'CCGG_A
Cuts at: 1226 1226
Size: 5666
BspHI T'CATG_A
Cuts at: 4098 4098
Size: 5666
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1948 2716 2930 3232 3613 1948
Size: 768 214 302 381 4001
Fragments arranged by size:
4001 768 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3958 4012 5496 5661 50
Size: 3908 54 1484 165 55
Fragments arranged by size:
3908 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1173 3578 1173
Size: 2405 3261
BsrFI r'CCGG_y
Cuts at: 1868 3664 3845 1868
Size: 1796 181 3689
Fragments arranged by size:
3689 1796 181
BssSI C'ACGA_G
Cuts at: 3937 4645 3937
Size: 708 4958
BstAPI GCAn_nnn'nTGC
Cuts at: 366 1940 3043 366
Size: 1574 1103 2989
Fragments arranged by size:
2989 1574 1103
BstBI TT'CG_AA
Cuts at: 4027 4027
Size: 5666
BstEII G'GTnAC_C
Cuts at: 1309 2563 1309
Size: 1254 4412
BstXI CCAn_nnnn'nTGG
Cuts at: 996 996
Size: 5666
BstYI r'GATC_y
Cuts at: 1 405 551 641 1704 1815 2343 2691 3158
Size: 404 146 90 1063 111 528 348 467
Cuts at: 3158 3181 3516 3762 4068 4080 4166 5218 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1063 1052 528 467 449 404 348 335
306 246 146 111 90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 922 922
Size: 5666
BtgI C'CryG_G
Cuts at: 107 864 2591 107
Size: 757 1727 3182
Fragments arranged by size:
3182 1727 757
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3616 3797 3960 3616
Size: 181 163 5322
Fragments arranged by size:
5322 181 163
BtsI GCAGTG_nn'
Cuts at: 121 763 2086 2323 2909 121
Size: 642 1323 237 586 2878
Fragments arranged by size:
2878 1323 642 586 237
DraI TTT'AAA
Cuts at: 2665 2665
Size: 5666
DraIII CAC_nnn'GTG
Cuts at: 1375 1375
Size: 5666
DrdI GACnn_nn'nnGTC
Cuts at: 3372 4716 3372
Size: 1344 4322
EaeI y'GGCC_r
Cuts at: 321 1632 2580 3251 3425 3816 3843 5188 321
Size: 1311 948 671 174 391 27 1345 799
Fragments arranged by size:
1345 1311 948 799 671 391 174 27
EagI C'GGCC_G
Cuts at: 3251 3251
Size: 5666
EarI CTCTTCn'nnn_
Cuts at: 3689 3899 3689
Size: 210 5456
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2032 3874 4616 4762 5476 2032
Size: 1842 742 146 714 2222
Fragments arranged by size:
2222 1842 742 714 146
EcoICRI GAG'CTC
Cuts at: 853 2304 2678 853
Size: 1451 374 3841
Fragments arranged by size:
3841 1451 374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 920 955 964 1141 1453 1493 1528 1605
Size: 19 35 9 177 312 40 35 77
Cuts at: 1605 1810 1831 1896 1897 2143 2257 2281 2350
Size: 205 21 65 1 246 114 24 69
Cuts at: 2350 2392 2404 2508 3491 3923 4276 4938 901
Size: 42 12 104 983 432 353 662 1629
Fragments arranged by size:
1629 983 662 432 353 312 246 205
177 114 104 77 69 65 42 40
35 35 24 21 19 12 9 1
EcoO109I rG'GnC_Cy
Cuts at: 336 479 1578 1597 2446 2805 336
Size: 143 1099 19 849 359 3197
Fragments arranged by size:
3197 1099 849 359 143 19
EcoRI G'AATT_C
Cuts at: 2670 2670
Size: 5666
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 268 3685 268
Size: 3417 2249
FspI TGC'GCA
Cuts at: 3447 3447
Size: 5666
HaeII r_GCGC'y
Cuts at: 1869 3100 3348 4578 5349 1869
Size: 1231 248 1230 771 2186
Fragments arranged by size:
2186 1231 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 456 488 529 561 626 651 658
Size: 32 96 32 41 32 65 25 7
Cuts at: 658 683 1088 1120 1689 1721 2248 2280 2347
Size: 25 405 32 569 32 527 32 67
Cuts at: 2347 2379 3508 3538 3540 3570 328
Size: 32 1129 30 2 30 2424
Fragments arranged by size:
2424 1129 569 527 405 96 67 65
41 32 32 32 32 32 32 32
30 30 25 25 7 2
HincII GTy'rAC
Cuts at: 2705 2705
Size: 5666
HindIII A'AGCT_T
Cuts at: 2123 2123
Size: 5666
KasI G'GCGC_C
Cuts at: 3096 3344 3096
Size: 248 5418
KpnI G_GTAC'C
Cuts at: 2686 2686
Size: 5666
MfeI C'AATT_G
Cuts at: 484 1496 484
Size: 1012 4654
MluI A'CGCG_T
Cuts at: 5099 5099
Size: 5666
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 791 851 1502 1589 1834 4425 4609 791
Size: 60 651 87 245 2591 184 1848
Fragments arranged by size:
2591 1848 651 245 184 87 60
MscI TGG'CCA
Cuts at: 2582 3427 2582
Size: 845 4821
MslI CAynn'nnrTG
Cuts at: 1961 3655 3782 1961
Size: 1694 127 3845
Fragments arranged by size:
3845 1694 127
MspA1I CmG'CkG
Cuts at: 159 306 879 898 930 1656 2028 2235 2777
Size: 147 573 19 32 726 372 207 542
Cuts at: 2777 3091 3451 4235 4480 159
Size: 314 360 784 245 1345
Fragments arranged by size:
1345 784 726 573 542 372 360 314
245 207 147 32 19
NaeI GCC'GGC
Cuts at: 1870 3847 1870
Size: 1977 3689
NarI GG'CG_CC
Cuts at: 3097 3345 3097
Size: 248 5418
NcoI C'CATG_G
Cuts at: 107 107
Size: 5666
NgoMIV G'CCGG_C
Cuts at: 1868 3845 1868
Size: 1977 3689
NsiI A_TGCA'T
Cuts at: 2413 2413
Size: 5666
NspI r_CATG'y
Cuts at: 1080 1665 2719 3219 4822 1080
Size: 585 1054 500 1603 1924
Fragments arranged by size:
1924 1603 1054 585 500
PciI A'CATG_T
Cuts at: 4818 4818
Size: 5666
PflMI CCAn_nnn'nTGG
Cuts at: 297 870 297
Size: 573 5093
PfoI T'CCnGG_A
Cuts at: 547 554 1157 2159 547
Size: 7 603 1002 4054
Fragments arranged by size:
4054 1002 603 7
PmeI GTTT'AAAC
Cuts at: 2665 2665
Size: 5666
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 488 520 874 906 1477 1509 4112 4144 488
Size: 32 354 32 571 32 2603 32 2010
Fragments arranged by size:
2603 2010 571 354 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 336 2446 336
Size: 2110 3556
PspOMI G'GGCC_C
Cuts at: 479 1596 479
Size: 1117 4549
PstI C_TGCA'G
Cuts at: 131 359 707 2018 2713 3398 131
Size: 228 348 1311 695 685 2399
Fragments arranged by size:
2399 1311 695 685 348 228
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5666
PvuII CAG'CTG
Cuts at: 159 306 879 898 930 1656 3091 3451 159
Size: 147 573 19 32 726 1435 360 2374
Fragments arranged by size:
2374 1435 726 573 360 147 32 19
RsrII CG'GwC_CG
Cuts at: 3861 3861
Size: 5666
SacI G_AGCT'C
Cuts at: 855 2306 2680 855
Size: 1451 374 3841
Fragments arranged by size:
3841 1451 374
SalI G'TCGA_C
Cuts at: 2703 2703
Size: 5666
SapI GCTCTTCn'nnn_
Cuts at: 3689 3899 3689
Size: 210 5456
SbfI CC_TGCA'GG
Cuts at: 2713 2713
Size: 5666
ScaI AGT'ACT
Cuts at: 208 5183 208
Size: 4975 691
SfcI C'TryA_G
Cuts at: 33 127 355 703 1030 1692 2014 2709 3394
Size: 94 228 348 327 662 322 695 685
Cuts at: 3394 4362 4553 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 695 685 662 348 327 322
228 191 94
SfoI GGC'GCC
Cuts at: 3098 3346 3098
Size: 248 5418
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5666
SmaI CCC'GGG
Cuts at: 2116 2688 2116
Size: 572 5094
SmlI C'TyrA_G
Cuts at: 1108 2242 2821 3736 4450 4712 1108
Size: 1134 579 915 714 262 2062
Fragments arranged by size:
2062 1134 915 714 579 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5666
SphI G_CATG'C
Cuts at: 1080 1665 2719 3219 1080
Size: 585 1054 500 3527
Fragments arranged by size:
3527 1054 585 500
StuI AGG'CCT
Cuts at: 1640 1640
Size: 5666
StyI C'CwwG_G
Cuts at: 107 269 1574 2442 2800 3154 107
Size: 162 1305 868 358 354 2619
Fragments arranged by size:
2619 1305 868 358 354 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1938 2120 3249 3382 3841 4050 1938
Size: 182 1129 133 459 209 3554
Fragments arranged by size:
3554 1129 459 209 182 133
TatI w'GTAC_w
Cuts at: 206 5181 206
Size: 4975 691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 5183 5215 2078
Size: 32 3073 32 2529
Fragments arranged by size:
3073 2529 32 32
Tth111I GACn'n_nGTC
Cuts at: 1887 3463 1887
Size: 1576 4090
XbaI T'CTAG_A
Cuts at: 2697 2697
Size: 5666
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1715 1769 2409 3161 1715
Size: 54 640 752 4220
Fragments arranged by size:
4220 752 640 54
XmaI C'CCGG_G
Cuts at: 2114 2686 2114
Size: 572 5094
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AscI AvrII BaeI
BbvCI BmgBI BmtI Bpu10I BsiWI BsmI BsrGI BssHII
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI PacI PasI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI