Restriction map for
pF1KB3683
(Circular) (Six-base) MAPSORT of: pF1KB3683.seq Check: 8634 from: 1 to: 6635
pF1KB3683 6635 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1559 1559
Size: 6635
AatII G_ACGT'C
Cuts at: 1884 1884
Size: 6635
AccI GT'mk_AC
Cuts at: 1537 1974 2510 2919 3673 1537
Size: 437 536 409 754 4499
Fragments arranged by size:
4499 754 536 437 409
Acc65I G'GTAC_C
Cuts at: 3651 3651
Size: 6635
AclI AA'CG_TT
Cuts at: 6475 6475
Size: 6635
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 385 511 1925 2508 4460 4892 5639 5907
Size: 50 126 1414 583 1952 432 747 268
Cuts at: 5907 335
Size: 1063
Fragments arranged by size:
1952 1414 1063 747 583 432 268 126
50
AflIII A'CryG_T
Cuts at: 2605 3987 5091 6068 2605
Size: 1382 1104 977 3172
Fragments arranged by size:
3172 1382 1104 977
AgeI A'CCGG_T
Cuts at: 5023 5023
Size: 6635
AhdI GACnn_n'nnGTC
Cuts at: 3041 3041
Size: 6635
AleI CACnn'nnGTG
Cuts at: 3290 3290
Size: 6635
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1196 1228 1196
Size: 32 6603
AlwNI CAG_nnn'CTG
Cuts at: 3524 3940 5507 3524
Size: 416 1567 4652
Fragments arranged by size:
4652 1567 416
ApaLI G'TGCA_C
Cuts at: 5405 5405
Size: 6635
ApoI r'AATT_y
Cuts at: 377 560 786 1062 1335 1369 1563 1680 1735
Size: 183 226 276 273 34 194 117 55
Cuts at: 1735 1761 1772 2857 2908 3639 5988 6244 377
Size: 26 11 1085 51 731 2349 256 768
Fragments arranged by size:
2349 1085 768 731 276 273 256 226
194 183 117 55 51 34 26 11
AseI AT'TA_AT
Cuts at: 20 668 20
Size: 648 5987
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6635
AvaI C'yCGr_G
Cuts at: 3655 3655
Size: 6635
BamHI G'GATC_C
Cuts at: 645 3660 645
Size: 3015 3620
BanI G'GyrC_C
Cuts at: 844 3651 4065 4313 4348 844
Size: 2807 414 248 35 3131
Fragments arranged by size:
3131 2807 414 248 35
BanII G_rGCy'C
Cuts at: 3649 4679 3649
Size: 1030 5605
BbeI G_GCGC'C
Cuts at: 4069 4317 4069
Size: 248 6387
BbsI GAAGACnn'nnnn_
Cuts at: 293 2486 293
Size: 2193 4442
BciVI GTATCCnnnnn_n'
Cuts at: 1198 4533 5294 6635 1198
Size: 3335 761 1341 1198
Fragments arranged by size:
3335 1341 1198 761
BclI T'GATC_A
Cuts at: 1470 1916 2250 2577 4155 1470
Size: 446 334 327 1578 3950
Fragments arranged by size:
3950 1578 446 334 327
BglI GCCn_nnn'nGGC
Cuts at: 1693 1693
Size: 6635
BglII A'GATC_T
Cuts at: 3340 4150 6187 3340
Size: 810 2037 3788
Fragments arranged by size:
3788 2037 810
BlpI GC'TnA_GC
Cuts at: 320 3747 320
Size: 3427 3208
Bme1580I G_kGCm'C
Cuts at: 4260 4353 5409 4260
Size: 93 1056 5486
Fragments arranged by size:
5486 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1473 3402 3847 3928 4263 6407 1473
Size: 1929 445 81 335 2144 1701
Fragments arranged by size:
2144 1929 1701 445 335 81
BmtI G_CTAG'C
Cuts at: 1594 1594
Size: 6635
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5002 5002
Size: 6635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 2126 2657 1520
Size: 606 531 5498
Fragments arranged by size:
5498 606 531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 3811 4690 5182 5480 802
Size: 3009 879 492 298 1957
Fragments arranged by size:
3009 1957 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1174 1639 6339 1174
Size: 465 4700 1470
Fragments arranged by size:
4700 1470 465
BsaAI yAC'GTr
Cuts at: 464 2608 3988 464
Size: 2144 1380 3111
Fragments arranged by size:
3111 2144 1380
BsaBI GATnn'nnATC
Cuts at: 6 193 614 1891 1945 3446 4149 6186 6
Size: 187 421 1277 54 1501 703 2037 455
Fragments arranged by size:
2037 1501 1277 703 455 421 187 54
BsaHI Gr'CG_yC
Cuts at: 1881 4066 4314 5016 6512 1881
Size: 2185 248 702 1496 2004
Fragments arranged by size:
2185 2004 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3824 4345 5023 5297 5444 5855 3824
Size: 521 678 274 147 411 4604
Fragments arranged by size:
4604 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2684 2714 2684
Size: 30 6605
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 284 848 1094 284
Size: 564 246 5825
Fragments arranged by size:
5825 564 246
BseYI C'CCAG_C
Cuts at: 1294 1823 4060 4818 5395 5891 1294
Size: 529 2237 758 577 496 2038
Fragments arranged by size:
2237 2038 758 577 529 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3151 3151
Size: 6635
BsiEI CG_ry'CG
Cuts at: 104 4177 4223 5431 104
Size: 4073 46 1208 1308
Fragments arranged by size:
4073 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 420 2755 3649 4427 4617 5409 420
Size: 2335 894 778 190 792 1646
Fragments arranged by size:
2335 1646 894 792 778 190
BsmI GAATG_Cn'
Cuts at: 640 1309 3061 3520 3563 640
Size: 669 1752 459 43 3712
Fragments arranged by size:
3712 1752 669 459 43
BsmBI CGTCTCn'nnnn_
Cuts at: 1574 1872 2800 1574
Size: 298 928 5409
Fragments arranged by size:
5409 928 298
Bsp1286I G_dGCh'C
Cuts at: 420 2755 3649 4260 4353 4427 4617 4679 5409
Size: 2335 894 611 93 74 190 62 730
Cuts at: 5409 420
Size: 1646
Fragments arranged by size:
2335 1646 894 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 5855 5855
Size: 6635
BspHI T'CATG_A
Cuts at: 2492 5811 2492
Size: 3319 3316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1559 3685 3899 4201 4582 1559
Size: 2126 214 302 381 3612
Fragments arranged by size:
3612 2126 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4927 4981 6465 6630 50
Size: 4877 54 1484 165 55
Fragments arranged by size:
4877 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 2450 4547 2141
Size: 309 2097 4229
Fragments arranged by size:
4229 2097 309
BsrFI r'CCGG_y
Cuts at: 4633 4814 5023 4633
Size: 181 209 6245
Fragments arranged by size:
6245 209 181
BsrGI T'GTAC_A
Cuts at: 3191 3191
Size: 6635
BssSI C'ACGA_G
Cuts at: 3290 3344 4906 5264 3290
Size: 54 1562 358 4661
Fragments arranged by size:
4661 1562 358 54
BstAPI GCAn_nnn'nTGC
Cuts at: 1789 3524 4012 1789
Size: 1735 488 4412
Fragments arranged by size:
4412 1735 488
BstBI TT'CG_AA
Cuts at: 4996 4996
Size: 6635
BstYI r'GATC_y
Cuts at: 1 645 987 1287 1355 1711 1842 1946 2070
Size: 644 342 300 68 356 131 104 124
Cuts at: 2070 3311 3340 3441 3660 4127 4150 4485 4731
Size: 1241 29 101 219 467 23 335 246
Cuts at: 4731 5743 5829 5841 6187 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1241 1012 644 467 449 356 346 342
335 300 246 219 131 124 104 101
86 68 29 23 12
Bsu36I CC'TnA_GG
Cuts at: 1867 1867
Size: 6635
BtgI C'CryG_G
Cuts at: 2514 3329 3396 2514
Size: 815 67 5753
Fragments arranged by size:
5753 815 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4585 4766 4929 4585
Size: 181 163 6291
Fragments arranged by size:
6291 181 163
BtsI GCAGTG_nn'
Cuts at: 3878 3878
Size: 6635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 341 376 3453 3488 341
Size: 35 3077 35 3488
Fragments arranged by size:
3488 3077 35 35
DraI TTT'AAA
Cuts at: 1562 3634 1562
Size: 2072 4563
DraIII CAC_nnn'GTG
Cuts at: 2194 2194
Size: 6635
DrdI GACnn_nn'nnGTC
Cuts at: 1533 2391 4341 5199 1533
Size: 858 1950 858 2969
Fragments arranged by size:
2969 1950 858 858
EaeI y'GGCC_r
Cuts at: 2078 2447 4220 4394 4785 4812 6157 2078
Size: 369 1773 174 391 27 1345 2556
Fragments arranged by size:
2556 1773 1345 391 369 174 27
EagI C'GGCC_G
Cuts at: 4220 4220
Size: 6635
EarI CTCTTCn'nnn_
Cuts at: 847 1239 2011 4658 4868 847
Size: 392 772 2647 210 2614
Fragments arranged by size:
2647 2614 772 392 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4843 5153 5299 6445 4843
Size: 310 146 1146 5033
Fragments arranged by size:
5033 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3647 3647
Size: 6635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 385 511 1520 1925 2126 2508 2657 4460
Size: 50 126 1009 405 201 382 149 1803
Cuts at: 4460 4892 5639 5907 335
Size: 432 747 268 1063
Fragments arranged by size:
1803 1063 1009 747 432 405 382 268
201 149 126 50
EcoO109I rG'GnC_Cy
Cuts at: 3186 3301 3500 3774 3186
Size: 115 199 274 6047
Fragments arranged by size:
6047 274 199 115
EcoRI G'AATT_C
Cuts at: 786 3639 786
Size: 2853 3782
EcoRV GAT'ATC
Cuts at: 3000 3000
Size: 6635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 541 4654 541
Size: 4113 2522
FspI TGC'GCA
Cuts at: 4416 4416
Size: 6635
HaeII r_GCGC'y
Cuts at: 4069 4317 5339 6318 4069
Size: 248 1022 979 4386
Fragments arranged by size:
4386 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 522 554 757 789 1272 1304 2300 2332 3027
Size: 32 203 32 483 32 996 32 695
Cuts at: 3027 3059 3129 3161 3273 3305 3325 3357 4477
Size: 32 70 32 112 32 20 32 1120
Cuts at: 4477 4507 4509 4539 522
Size: 30 2 30 2618
Fragments arranged by size:
2618 1120 996 695 483 203 112 70
32 32 32 32 32 32 32 32
30 30 20 2
HincII GTy'rAC
Cuts at: 581 2920 3126 3674 581
Size: 2339 206 548 3542
Fragments arranged by size:
3542 2339 548 206
HindIII A'AGCT_T
Cuts at: 1028 1028
Size: 6635
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 6635
KasI G'GCGC_C
Cuts at: 4065 4313 4065
Size: 248 6387
KpnI G_GTAC'C
Cuts at: 3655 3655
Size: 6635
MfeI C'AATT_G
Cuts at: 2032 2441 2032
Size: 409 6226
MluI A'CGCG_T
Cuts at: 6068 6068
Size: 6635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 971 1689 1809 5306 5490 971
Size: 718 120 3497 184 2116
Fragments arranged by size:
3497 2116 718 184 120
MscI TGG'CCA
Cuts at: 2080 2449 4396 2080
Size: 369 1947 4319
Fragments arranged by size:
4319 1947 369
MslI CAynn'nnrTG
Cuts at: 329 919 1160 1897 3290 3509 3513 4624 4751
Size: 590 241 737 1393 219 4 1111 127
Cuts at: 4751 329
Size: 2213
Fragments arranged by size:
2213 1393 1111 737 590 241 219 127
4
MspA1I CmG'CkG
Cuts at: 237 1302 2506 2516 3746 4060 4420 5433 5678
Size: 1065 1204 10 1230 314 360 1013 245
Cuts at: 5678 237
Size: 1194
Fragments arranged by size:
1230 1204 1194 1065 1013 360 314 245
10
NaeI GCC'GGC
Cuts at: 4816 4816
Size: 6635
NarI GG'CG_CC
Cuts at: 4066 4314 4066
Size: 248 6387
NcoI C'CATG_G
Cuts at: 3329 3396 3329
Size: 67 6568
NdeI CA'TA_TG
Cuts at: 3266 3510 3266
Size: 244 6391
NgoMIV G'CCGG_C
Cuts at: 4814 4814
Size: 6635
NheI G'CTAG_C
Cuts at: 1590 1590
Size: 6635
NspI r_CATG'y
Cuts at: 185 328 825 2618 2635 3572 3688 4188 5095
Size: 143 497 1793 17 937 116 500 907
Cuts at: 5095 185
Size: 1725
Fragments arranged by size:
1793 1725 937 907 500 497 143 116
17
PciI A'CATG_T
Cuts at: 5091 5091
Size: 6635
PflMI CCAn_nnn'nTGG
Cuts at: 2899 2899
Size: 6635
PmeI GTTT'AAAC
Cuts at: 3634 3634
Size: 6635
PmlI CAC'GTG
Cuts at: 2608 2608
Size: 6635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5774 5806 5774
Size: 32 6603
PpuMI rG'GwC_Cy
Cuts at: 3186 3301 3500 3186
Size: 115 199 6321
Fragments arranged by size:
6321 199 115
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3184 3216 3184
Size: 32 6603
PstI C_TGCA'G
Cuts at: 1785 3682 4367 1785
Size: 1897 685 4053
Fragments arranged by size:
4053 1897 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6635
PvuII CAG'CTG
Cuts at: 237 2506 4060 4420 237
Size: 2269 1554 360 2452
Fragments arranged by size:
2452 2269 1554 360
RsrII CG'GwC_CG
Cuts at: 4830 4830
Size: 6635
SacI G_AGCT'C
Cuts at: 3649 3649
Size: 6635
SacII CC_GC'GG
Cuts at: 2517 2517
Size: 6635
SalI G'TCGA_C
Cuts at: 2918 3672 2918
Size: 754 5881
SapI GCTCTTCn'nnn_
Cuts at: 4658 4868 4658
Size: 210 6425
SbfI CC_TGCA'GG
Cuts at: 3682 3682
Size: 6635
ScaI AGT'ACT
Cuts at: 807 6152 807
Size: 5345 1290
SfcI C'TryA_G
Cuts at: 33 299 307 1664 1781 2058 2507 2781 3678
Size: 266 8 1357 117 277 449 274 897
Cuts at: 3678 4363 5356 5547 33
Size: 685 993 191 1121
Fragments arranged by size:
1357 1121 993 897 685 449 277 274
266 191 117 8
SfoI GGC'GCC
Cuts at: 4067 4315 4067
Size: 248 6387
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6635
SmaI CCC'GGG
Cuts at: 3657 3657
Size: 6635
SmlI C'TyrA_G
Cuts at: 781 3790 4705 5197 5459 781
Size: 3009 915 492 262 1957
Fragments arranged by size:
3009 1957 915 492 262
SpeI A'CTAG_T
Cuts at: 67 2800 67
Size: 2733 3902
SphI G_CATG'C
Cuts at: 185 328 825 2635 3572 3688 4188 185
Size: 143 497 1810 937 116 500 2632
Fragments arranged by size:
2632 1810 937 500 497 143 116
SspI AAT'ATT
Cuts at: 3230 3230
Size: 6635
StyI C'CwwG_G
Cuts at: 2217 3329 3396 3769 4123 2217
Size: 1112 67 373 354 4729
Fragments arranged by size:
4729 1112 373 354 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4218 4351 4810 5019 4218
Size: 133 459 209 5834
Fragments arranged by size:
5834 459 209 133
TatI w'GTAC_w
Cuts at: 805 3191 6150 805
Size: 2386 2959 1290
Fragments arranged by size:
2959 2386 1290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6152 6184 6152
Size: 32 6603
Tth111I GACn'n_nGTC
Cuts at: 2924 4432 2924
Size: 1508 5127
XbaI T'CTAG_A
Cuts at: 3666 3666
Size: 6635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2088 4130 2088
Size: 2042 4593
XmaI C'CCGG_G
Cuts at: 3655 3655
Size: 6635
XmnI GAAnn'nnTTC
Cuts at: 524 683 3059 524
Size: 159 2376 4100
Fragments arranged by size:
4100 2376 159
ZraI GAC'GTC
Cuts at: 1882 1882
Size: 6635
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PciI PflMI PmeI PmlI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AlfI ApaI AscI AvrII BaeI BbvCI
BcgI BfrBI BmgBI Bpu10I BsiWI BssHII BstEII BstXI
BstZ17I ClaI EcoNI FseI FspAI NotI NruI NsiI
PacI PasI PfoI PshAI PsiI PspOMI PspXI SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI